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'Biosynthesis' in keywords Facet   Publication Year 1981  [X]
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Publication Year
1981[X]
1Author    Rainer Sütfeld, Rolf WiermannRequires cookie*
 Title    Purification of Chalcone Synthase from Tulip Anthers and Comparison with the Synthase from Cosmos Petals  
 Abstract    Chalcone synthase was isolated from both anthers of Tulipa cv. "A peldoorn" and petals of Cosmos sulphureus Cav. After certain prepurification steps, the enzymes were further purified using gel chrom atography on Sephadex G-200 followed by repeated hydroxylapatite absorption chromatography. Both the enzymes showed the same chrom atographic properties. After gel chrom atography as well as after the first hydroxylapatite fractionation, the reaction products appeared as flavanones. However, after the second hydroxylapatite step, production of chalcones was observed. Like the enzyme from tulip anthers, the synthase from Cosmos petals produced the correspondingly substituted chalcones when p-coumaroyl-CoA, caffeoyl-CoA and feruloyl-CoA, respectively, were used as substractes. In both the cases, the ratios o f the different chalcones produced were found to be about the same. The appearance o f chalcone synthesis in this in vitro assay is caused by the complete elim ination of chalcone isomerase in the purification procedure. The importance of the isomerase for flavonoid biosynthesis, particularly in plant systems which are accumulating chalcones, is discussed. 
  Reference    Z. Naturforsch. 36c, 30—3 (1981); received N ovem ber 12 1980 
  Published    1981 
  Keywords    Tulipa, Cosmos, Biosynthesis, Flavonoids, Chalcone Synthase 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0030.pdf 
 Identifier    ZNC-1981-36c-0030 
 Volume    36 
2Author    W.-U Mutert, H. Lütfring, W. Barz, D. StrackRequires cookie*
 Title    of the Genus Fusarium  
 Abstract    Among various Fusarium strains tested Gibberella fuji-kuroi (SAW) WR was shown to be a high producer o f the phytotoxin fusaric acid. 
  Reference    Z. Naturforsch. 36c, 338 (1981); received January 19 1981 
  Published    1981 
  Keywords    Fusaric Acid, Fusarium, Gibberella, Biosynthesis, High Performance Liquid Chromatography 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0338_n.pdf 
 Identifier    ZNC-1981-36c-0338_n 
 Volume    36 
3Author    Jan Van Brederode, RiaK. Am, Ps-H EinsbroekRequires cookie*
 Title    Structure and Biosynthesis o f Vitexin 2"-0-Xyloside in Silene alba  
 Abstract    In petals o f Silene alba plants o f Armenian populations, vitexin 2"-0-xyloside was demonstrated to be present. The enzyme, catalyzing the transfer of the xylose moiety of UDP-xylose to vitexin, has its optimal activity at pH 7.8. Mg2+ and Mn2+ stimulated the reaction. The K m values were 2 x 10-5 M for vitexin and 3 x 10-5 M for UDP-xylose. 
  Reference    Z. Naturforsch. 36c, 484 (1981); received February 20 1981 
  Published    1981 
  Keywords    Silene, Caryophyllaceae, Biosynthesis, Vitexin 2"-0-Xyloside, Xylosyltransferase 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0484_n.pdf 
 Identifier    ZNC-1981-36c-0484_n 
 Volume    36 
4Author    Jan Van Brederode, Ria Kamps-HeinsbroekRequires cookie*
 Title    Methylation of 3',4' Di-O H C-Glycosyl- flavones in Silene  
 Abstract    In green parts of Silene plants of the genotype g lR two methylated flavonoids were found: isoscoparin and isosco-parin 2"-0-rhamnoside. An enzyme has been demonstrated to catalyze the transfer of the methyl moiety of S-adenosyl methionine to iso-orientin and iso-orientin 2"-0-rhamnosi-de. Maximal activity takes place at pH 8.0 -8 .2 . Of the me­ tal ions Mn2+, Mg2+, Ca2+, Co2+, Zn2+ and Hg2+, only Co2+ stimulated the reaction at conc. > 2 m M . For the methyla­ tion o f isoorientin the K m values were 4 x 10-6 M for S-ade-nosyl methionine and 0.32 x 10~3 m for iso-orientin. When isoorientin 2"-0-rhamnoside was used as substrate the K m values were 5 x 10"6 m for S-adenosylmethionine and 7 x 10-6 M for iso-orientin 2"-0-rhamnoside. 
  Reference    Z. Naturforsch. 36c, 486 (1981); received February 20 1981 
  Published    1981 
  Keywords    Silene, Caryophyllaceae, Methylation, Biosynthesis, Iso-scoparin 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0486_n.pdf 
 Identifier    ZNC-1981-36c-0486_n 
 Volume    36 
5Author    L. Britsch, W. Heller, H. GrisebachRequires cookie*
 Title    Conversion of Flavanone to Flavone, Dihydroflavonol and Flavonol with an Enzyme System from Cell Cultures of Parsley  
 Abstract    Soluble enzyme preparations from irradiated cell suspension cultures of parsley (Petroselinum hortense Hoffm.) catalyse the conversion o f flavanone to flavone, dihydroflavonol and flavonol. These reactions require 2-oxoglutarate, Fe2+ and ascorbate as cofactors. In the presence o f these cofactors conversion of dihydroflavonol to flavonol was also observed. With this system in vitro biosynthesis of radioactive flavone, dihydroflavonol and flavonol from [2-14C]malonyl-CoA and 4-coumaroyl-CoA in good yield and with high specific activity is possible. We postulate that synthesis of flavone and flavonol from flavanone proceeds via 2-hydroxy-and 2,3-dihydroxyflavanone, respectively, with subsequent dehydration. The microsomal fraction of the parsley cells contains an NADPH-dependent flavanone 3'-hydroxylase. 
  Reference    Z. Naturforsch. 36c, 742—750 (1981); received June 3 1981 
  Published    1981 
  Keywords    Flavonoids, Biosynthesis, Dioxygenases, Petroselinum hortense, Cell Cultures 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0742.pdf 
 Identifier    ZNC-1981-36c-0742 
 Volume    36 
6Author    Gary Kuroki, JonathanE. PoultonRequires cookie*
 Title    The Para-O-Methylation of Apigenin to Acacetin by Cell-Free Extracts of Robinia pseudoacacia L  
 Abstract    Crude extracts from young Robinia pseudoacacia seedlings, shoots, and callus tissue catalyze the />ara-0-methylation of apigenin to acacetin using S-adenosyl-L-methionine as methyl donor. Optimum activity was exhibited at pH 9.0, and Mg2+ was not required for maximum activity. EDTA (10 m M) did not affect the reaction rate, but 47% inhibition was observed with SAH (100 u m). /?-Mercaptoethanol (5 m M) was required in the homogenization medium for optimum O-methyltransferase activity. Apigenin (K m, 50 hm) was the best substrate, but significant activity was shown towards caffeic acid, 5-hydroxyferulic acid, naringenin, and quercetin. Para-coumaric, ferulic, and sinapic acids were not methylated. The Km for S-adenosyl-L-methionine was 31 hm. Our demonstration o f a /wra-O-methyltransferase activity methylating apigenin, but not /rara-coumaric acid, strongly supports the conclusion that the B-ring methylation pattern of acacetin is determined at the C15-level in Robinia pseudoacacia. 
  Reference    Z. Naturforsch. 36c, 916 (1981); received July 6 1981 
  Published    1981 
  Keywords    Robinia pseudoacacia, Biosynthesis, Flavonoids, Para-O-Methyltransferase, Acacetin 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0916.pdf 
 Identifier    ZNC-1981-36c-0916 
 Volume    36 
7Author    H.-R SchultenRequires cookie*
 Title      
 Abstract    A combined 13 C nuclear magnetic resonance and field desorption mass spectrometric investigation of algae grown on 13 C02 has shown that the isotopic enrichment of amino acids extracted therefrom is neither uniform nor statistical. The use of these two indepen-dent techniques allows a new, detailed and accurate insight into the label distribution resulting from biosynthesis. The observed deviations from the statistical abundances are systematic. A system for classifying each member of the complex ensemble of isotopic species has been devised, so that the isotopomers may be ordered according to their relative probability of occurrence. 
  Reference    Z. Naturforsch. 36b, 1289—1296 (1981); received May 20 1981 
  Published    1981 
  Keywords    13 C Enriched Amino Acids, Biosynthesis, Label Distribution, NMR Spectra, Field Desorption Mass Spectrometry 
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 TEI-XML for    default:Reihe_B/36/ZNB-1981-36b-1289.pdf 
 Identifier    ZNB-1981-36b-1289 
 Volume    36 
8Author    Hamako Obata-Sasamoto, Atsushi Komamine, Koshi SaitoRequires cookie*
 Title    Biosynthesis of 3-Carboxy-6,7-dihydroxy-l, 2,3,4-tetra- hydroisoquinoline and l-Methyl-3-carboxy-6,7-dihydroxy-l, 2,3,4- tetrahydroisoquinoline in a Callus Culture of Stizolobium hassjoo  
 Abstract    dl-[/?-14C] 3,4-Dihydroxyphenylalanine (DOPA), [2-14C]pyruvate and [2-14C]acetate were ad­ ministered to a callus culture o f S. hassjoo and incorporation of the radioactivity into 3-carboxy-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline (I) and l-methyl-3-carboxy-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline (II) was examined. Incorporation of radioactivity from labelled DOPA into I and II, and from acetate into I were observed, while that from pyruvate into I and II and from acetate into II were hardly detected. The biosynthetic pathways o f I and D were discussed. 
  Reference    Z. Naturforsch. 36c, 921 (1981); received August 21 1981 
  Published    1981 
  Keywords    Stizolobium hassjoo, Biosynthesis, 3-Carboxy-6, 7-dihydroxy-1, 2, 3, 4-tetrahydroisoquinoline, 1 -Methyl-3-carboxy-6, 7-dihydroxy-1, 2, 3, 4-tetrahydroisoquinoline, Callus Culture 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0921.pdf 
 Identifier    ZNC-1981-36c-0921 
 Volume    36