| | 1 | Author
| Dagmar Busmann, RalfG. Ünter Berger | Requires cookie* | | | Title
| Oxyfunctionalization o f a-and ß-Pinene by Selected Basidiomycetes  | | | | Abstract
| Several strains of basidiom ycetes were examined for their ability to transform a-and ß-pinene in agitated submerged cultures. Four major metabolites of a-pinene (verbenol, ver-benone, myrtenol, and rrorcs-pinocarveol) and three main metabolites of ß-pinene (1,4-cineol, myrtenol, and rrarcs-pinocarveol) were isolated from the fermentation broth. The metabolic pathways included allylic oxidation, oxidative cleavage and further regioselective oxidation. G anoderm a applanatum was found to carry out the stereoselective allylic hydroxylation of a-pinene to verbenol, and o f ß-pinene to trans-pinocarveol in trans position to the C -C bridge. The optimal conditions of the bioreaction were established with respect to substrate concentration, incubation time and conversion time. D ue to growth inhibition caused by elevated substrate concentration, the bioconversion of ß-pinene required pre-grown cultures. Generally, mycelial pellet cultures were supplemented with the terpene substrate when a residual glucose content of 50% was reached. Depending on strain this point was reached after about 48 h. A n incubation period of two to three days gave best yields. The transient accumulation of oxygenated products apparently reflected different reaction velocities of the successive catabolic steps. | | | |
Reference
| Z. Naturforsch. 49c, 545 (1994); received May 26/June 20 1994 | | | |
Published
| 1994 | | | |
Keywords
| Pinene, Cineol, Basidiomycetes, Bioconversion, Transformation | | | |
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| default:Reihe_C/49/ZNC-1994-49c-0545.pdf | | | | Identifier
| ZNC-1994-49c-0545 | | | | Volume
| 49 | |
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