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'Basic Polyam inoacids Periodate Treatment' in keywords
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1978 (1)
1Author    G. Uhlenbruck, B. P. Chatterjee, U. SchuldesRequires cookie*
 Title    Schiff Base Form ation: Dem onstrated by a Precipitin Reaction in A g a r  
 Abstract    High-m olecular weight polyaldehydes, which can be o b ­ tained by periodate oxidation of glycosubstances, especially polysaccharides, do precipitate in a specific way by Schiff base formation, when diffusing in agar gel against high-m olecular weight, naturally occurring polyamines. album (m istletoe), R icinus com m unis and from the bivalve clam Tridacna m axim a (see Fig. l a) . Also the plant lectins from Ononis spinosa and Abrus precatorius do give such a precipitin reaction. This precipitation phenomenon however is abolished, when the terminal D-galactose (/?-l-6 linked) has been oxidized by sodium periodate (F ig . l b) , whereas the precipitin reaction due to Schiff base form ation does not occur with the genuine, un­ treated arabinogalactan (F ig . 1 a) . N egative reac­ tions are also seen after borohydride reduction or by blocking the carbonyl groups (o r the amino groups) by other methods [ 1 1 ] *. Oxidation o f lymphocytes from a variety of spe­ cies with either sodium periodate, galactose-oxidase or neuraminidase and galactose-oxidase induces blastogenic transformation [ 1 — 5 ], or lymphocyte cytotoxicity [ 6]. In addition, tumor cell killing occurs, when by the same procedures artificial con­ tacts are established between normal macrophages and tumor cells [ 7 ]. Although these intriguing phenomena are not yet completely elucidated, it is assumed that cross-linkages via Schiff bases play an important role [6 — 8]. The only evidence for this hypothesis is given by the fact, that sodium b oro­ hydride treatment abolishes the effect. It was there­ fore desirable, to have an in v itro model for the form ation o f Schiff bases in order to study the inter­ action o f different membrane glycosubstances and proteins. A s standard materials for developing a representative precipitin experiment we have first chosen periodate-treated arabinogalactan from plant origin [9 ] and polylysine as amino group donator [10]. In Fig. 1 it is demonstrated, that Schiff base fo r ­ mation between these two components indeed can be made visible in form o f a precipitation line. In addition, b iological substances with free amino groups, like histone [ 10], lysozyme [ 10] and haem oglobin [1 1 ] react in a similar way (F ig . 1 b), while a synthetic basic polym er without any amino groups, namely polybrene [ 10], does not pre­ cipitate as has been found by us. On the other hand, genuine arabinogalactan precipitates with some anti-galactosyl specific lectins like the one from Viscum 
  Reference    Z. Naturforsch. 33c, 442 (1978); received February 2 1978 
  Published    1978 
  Keywords    Basic Polyam inoacids Periodate-Treatment, Arabino-galactan, Lectins, Cell Aggregation 
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 TEI-XML for    default:Reihe_C/33/ZNC-1978-33c-0442_n.pdf 
 Identifier    ZNC-1978-33c-0442_n 
 Volume    33