| | 1 | Author
| D. W. Erner, R. Stripf | Requires cookie* | | | Title
| Differentiation of Rhizobium japonicum, I. Enzymatic Comparison of Nitrogenase Repressed and Derepressed Free Living Cells and of Bacteroids  | | | | Abstract
| Derepressed free living cells of Rhizobium japonicum strain 61-A-101 with leucine as single nitrogen source develop a maximum nitrogenase activity of 180 nmol C2H4-mg protein-1 -h _1 in liquid culture under 2% 0 2 in the gas phase. Only \0% of this activity is found with no oxygen in the gas phase during a 90 min incubation period. The maximum activity under 2% oxygen in the gas phase is unaffected by addition of 1 —100 m M NH4+ and by addition of low concentrations of glutamine (0.36 —1.44 m M) . Specific activities of alanine dehydrogenase (E.C. 1.4.1.1.) aspartate aminotransferase (E.C. 2.6.1.1.) and, with much lower activities, of GOGAT (E.C. 1.4.1.13) in nitrogenase active free living cells are more similar to bacteroids than to nitrogenase repressed free living cells from liquid culture. The activities in nitrogenase repressed cells were about 50% lower. Glutamine synthetase (E.C. 6.3.1.2.) activity in bacteroids and in nitrogenase active cells were also similar, but only about 25 — 30% of that found in nitrogenase repressed Rhizobium japonicum cells. | | | |
Reference
| Z. Naturforsch. 33c, 245 (1978); received February 9 1978 | | | |
Published
| 1978 | | | |
Keywords
| Rhizobium japonicum, Bacteroids, Derepressed Nitrogenase, Differentiation, N-Metabolism | | | |
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| default:Reihe_C/33/ZNC-1978-33c-0245.pdf | | | | Identifier
| ZNC-1978-33c-0245 | | | | Volume
| 33 | |
| 3 | Author
| John Wilcockson, Dietrich Werner | Requires cookie* | | | Title
| On the DNA Content of the Bacteroids of Rhizobium japonicum | | | | Abstract
| The DNA content of bacteroids from large nodules of soybean plants infected with Rhizobium japonicum strain 61 -A -101 was found to be 1.20 x 10~14 g per cell. Bacteroids from smaller nodules had slightly less DNA as did the stationary phase, free-living cells; both giving a value of 0.92 x 10-14 g per cell. In comparing these data with those of other workers it was found that there is little evidence to support suggestions that bacteroids possess anything less than a full genetic complement of DNA and that some misinterpretation of older published data has occurred. | | | |
Reference
| Z. Naturforsch. 34c, 793 (1979); received June 15 1979 | | | |
Published
| 1979 | | | |
Keywords
| DNA Content, Nodules, Bacteroids, Rhizobium, Soybean | | | |
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| default:Reihe_C/34/ZNC-1979-34c-0793.pdf | | | | Identifier
| ZNC-1979-34c-0793 | | | | Volume
| 34 | |
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