| | 1 | Author
| Jochen Schneider3, Kambiz Taraz3, H. Erbert Budzikiewicz3, MagaliD. Eleu, Philippe Thonartb, Philippe Jacquesb | Requires cookie* | | | Title
| The Structure of Two Fengycins from Bacillus subtilis S499  | | | | Abstract
| Fax: + 4 9 -2 2 1 -4 7 0 -5 0 5 7 . E-mail: h.budzikiewicz@uni-koeln.de The structures of the two fengycins, lipopeptides from Bacillus subtilis, were elucidated by spectroscopic methods and chemical degradation. They show a close structural relationship to the plipastatins from Bacillus cereus differing only in the stereochemistry of the Tyr residues. | | | |
Reference
| Z. Naturforsch. 54c, 859 (1999); received May 6/June 1 1999 | | | |
Published
| 1999 | | | |
Keywords
| Bacillus subtilis, Fengycin, Lipopeptides | | | |
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| default:Reihe_C/54/ZNC-1999-54c-0859.pdf | | | | Identifier
| ZNC-1999-54c-0859 | | | | Volume
| 54 | |
| 2 | Author
| M. H. Ecker, A.-M Dünger, G. Wachlin, F. Mach | Requires cookie* | | | Title
| Untersuchungen über die Regulation der Proteinsynthese auswachsender Sporen von Bacillus subtilis Studies on Protein Synthesis of Outgrowing Spores of Bacillus subtilis | | | | Abstract
| Two-dimensional gel electrophoresis according to O'Farrell [6] was used to study synthesis of membrane bound proteins during outgrowth of Bacillus subtilis spores. Three major classes of membrane bound proteins were distinguished, depending on the time of onset and duration of their synthesis: la — a great amount of proteins typical of vegetative cells made throughout all stages of outgrowth and vegetative growth; lb — vegetative proteins synthesized during middle and later outgrowth stages as well as during vegetative growth (sequential activation); II — outgrowth-specific proteins synthesized during early outgrowth but not in vegetative cells. A similar classification was made for total proteins [9]. In a temperature-sensitive mutant defective in early outgrowth the sequential activation of vegetative genes (group lb) is especially inhibited under restrictive conditions, whereas the syn thesis of group Ia-proteins and outgrowth-specific proteins (II) could be detected during early outgrowth stages. We suggest that the expression of outgrowth genes, whose products might be found among the outgrowth-specific proteins, could be involved in the sequential reactivation of vegetative genes during outgrowth. The vegetative proteins of group la (continuous synthesis) are produced throughout growth and differentiation. On the other hand the synthesis of vegetative proteins, group lb , is switched off during sporulation and switched on during outgrowth. The control mechanisms of the expression of these two groups of vegetative genes during growth and differen tiation are discussed. | | | |
Reference
| Z. Naturforsch. 40c, 421 (1985); eingegangen am 25. Oktober 1984/21. Februar 1985 | | | |
Published
| 1985 | | | |
Keywords
| Bacillus subtilis, Spore Outgrowth, Protein Synthesis, Membrane Proteins | | | |
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| default:Reihe_C/40/ZNC-1985-40c-0421.pdf | | | | Identifier
| ZNC-1985-40c-0421 | | | | Volume
| 40 | |
| 3 | Author
| Alexandru Popescu, Stefan Antohi, Stefan Trasculescu, Adriana Aurescu, NicolaeM. Anolescu, Victor Ciocnitu | Requires cookie* | | | Title
| Changes Induced by Pre-Fixation in Polycation Bacteriolysis and Surface Alterations Matching the Model of Wall Picnosis in Cell Lysis | | | | Abstract
| Pretreatments of B. subtilis and S. aureus cells with lower concentrations of fixative agents, led to modifications in bacteriolytic effect exerted by polyarginine and protamine: Glutaraldehyde blocked polycation bacteriolysis while formaldehyde and osmium tetroxide (0 s0 4) having no influence on polyarginine action, increased constantly the cell sensitivity to protamine in lower doses otherwise nonlytic; the sensitizing action also resulted in the extension of protamine bacteriolytic pattern including several staphylococcal strains; higher bacteriolytic doses of protamine were contrastively unable to lyse OsO? prefixed cells and gave an inconstant lytic value with formaldehyde treated bacteria. With higher concentrations, 0 s 0 4 preserved intactly its sensitizing action while formaldehyde displayed a decrease in its ability to sensitize B. subtilis cells to the lytic effect of protamine. Scaning electron microscopy of polycation treated cells showed prelytic lesions as surface granulations, shape and size modifications and cell splits. The interpretation of the results in terms of intra-and intermolecular adducts accompanied by con formational changes in surface macromolecules is discussed. It is concluded that the results match the model of polycation bacteriolysis by wall multizonal picnosis leading to surface splits and thereby triggering cell-lysis. | | | |
Reference
| Z. Naturforsch. 35c, 805—810 (1980); received July 9 1980 | | | |
Published
| 1980 | | | |
Keywords
| Poly-L-arginine, Protamine, Bacillus subtilis, Staphylococcus aureus, Glutaraldehyde | | | |
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| default:Reihe_C/35/ZNC-1980-35c-0805.pdf | | | | Identifier
| ZNC-1980-35c-0805 | | | | Volume
| 35 | |
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