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'Avena sativa' in keywords Facet   Publication Year 1981  [X]
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1Author    Wolfgang Knogge, Gottfried Weissenböck, Dieter StrackRequires cookie*
 Title    Application of Liquid Chromatography to a Study on 4-Coumarate: Coenzyme A Ligase Activity  
 Abstract    This report describes the separation of components from a 4-coumarate: CoA ligase assay by means of liquid chromatography. With the aid o f polyamide column chromatography it is possible to enrich and isolate chromatographically and UV spectroscopically pure /»-coumaroyl-CoA using as a solvent 0.01% N H 4OH in methanol subsequent to water and methanol alone. High performance liquid chromatography on octadecylsilane-bonded silica stationary phase allows a discontinuous determination o f ligase activity. All components — ATP, Coenzyme A, p-coumaric acid, and the products AMP and p-coumaroyl-CoA — can be separated and accurately quantified within 20 min using a water-acetonitrile gradient, containing 1% phosphoric acid. The presented HPLC method may be used to affirm the accuracy of optical tests. 
  Reference    Z. Naturforsch. 36c, 197—199 (1981); received December 91980 
  Published    1981 
  Keywords    Avena sativa, Poaceae, Ligase, /?-Coumaroyl-CoA, Polyamide Column Chromatography, High Performance Liquid Chromatography 
  Similar Items    Find
 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0197.pdf 
 Identifier    ZNC-1981-36c-0197 
 Volume    36 
2Author    Margareta Proksch, Dieter Strack, Gottfried WeissenböckRequires cookie*
 Title    Incorporation of [I4C]Phenylalanine and [14C|Cinnamic Acid into Leaf Pieces and Mesophyll Protoplasts from Oat Primary Leaves for Studies on Flavonoid Metabolism at the Tissue and Cell Level  
 Abstract    When the abaxial epidermis was peeled from 5 to 6 day old oat primary leaves, and 3 cm segments were floated on radioactive phenylalanine or cinnamic acid solutions, more than 90 per cent of the radioactivity was incorporated within 3 to 7 h depending on the developmental stage of the leaf. C-glycosylflavones were labelled within 15 min and radioactivity in these compounds increased for several hours. Pulse labelling and pulse chase experiments with either phenylalanine or cinnamic acid, unequivocally demonstrate that oat flavones are stable end products o f metabolism. However, this procedure does not distinguish between sequential biosynthesis of various flavones and their interconversion. Cinnamic acid was more efficiently (ca. 20 x) converted into oat leaf flavones than was phenylalanine, when the precursor was fed to leaf pieces, and flavones recovered from mesophyll protoplasts. Different labelling patterns were obtained with whole leaf segments and protoplasts which apparently reflect differences in tissue specific flavone biosynthesis o f mesophyll and epidermis. Isolated mesophyll protoplasts incubated with [14C]cinnamic acid synthesize 14C-labelled flavones characteristic o f the mesophyll, as well as several unidentified phenylpropanoid derivatives not found in the intact tissue. Data suggest that photosynthetically active mesophyll cells are a main site o f tissue specific flavone biosynthesis. 
  Reference    Z. Naturforsch. 36c, 222 (1981); received January 141981 
  Published    1981 
  Keywords    Avena sativa, Primary Leaf Tissues, Protoplasts, [14C]Phenylpropanoid Incorporation, C-Glycosyl-flavones 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0222.pdf 
 Identifier    ZNC-1981-36c-0222 
 Volume    36