| | 1 | Author
| Wolfgang Knogge, Gottfried Weissenböck, Dieter Strack | Requires cookie* | | | Title
| Application of Liquid Chromatography to a Study on 4-Coumarate: Coenzyme A Ligase Activity  | | | | Abstract
| This report describes the separation of components from a 4-coumarate: CoA ligase assay by means of liquid chromatography. With the aid o f polyamide column chromatography it is possible to enrich and isolate chromatographically and UV spectroscopically pure /»-coumaroyl-CoA using as a solvent 0.01% N H 4OH in methanol subsequent to water and methanol alone. High performance liquid chromatography on octadecylsilane-bonded silica stationary phase allows a discontinuous determination o f ligase activity. All components — ATP, Coenzyme A, p-coumaric acid, and the products AMP and p-coumaroyl-CoA — can be separated and accurately quantified within 20 min using a water-acetonitrile gradient, containing 1% phosphoric acid. The presented HPLC method may be used to affirm the accuracy of optical tests. | | | |
Reference
| Z. Naturforsch. 36c, 197—199 (1981); received December 91980 | | | |
Published
| 1981 | | | |
Keywords
| Avena sativa, Poaceae, Ligase, /?-Coumaroyl-CoA, Polyamide Column Chromatography, High Performance Liquid Chromatography | | | |
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| | | | | TEI-XML for
| default:Reihe_C/36/ZNC-1981-36c-0197.pdf | | | | Identifier
| ZNC-1981-36c-0197 | | | | Volume
| 36 | |
| 2 | Author
| Margareta Proksch, Dieter Strack, Gottfried Weissenböck | Requires cookie* | | | Title
| Incorporation of [I4C]Phenylalanine and [14C|Cinnamic Acid into Leaf Pieces and Mesophyll Protoplasts from Oat Primary Leaves for Studies on Flavonoid Metabolism at the Tissue and Cell Level | | | | Abstract
| When the abaxial epidermis was peeled from 5 to 6 day old oat primary leaves, and 3 cm segments were floated on radioactive phenylalanine or cinnamic acid solutions, more than 90 per cent of the radioactivity was incorporated within 3 to 7 h depending on the developmental stage of the leaf. C-glycosylflavones were labelled within 15 min and radioactivity in these compounds increased for several hours. Pulse labelling and pulse chase experiments with either phenylalanine or cinnamic acid, unequivocally demonstrate that oat flavones are stable end products o f metabolism. However, this procedure does not distinguish between sequential biosynthesis of various flavones and their interconversion. Cinnamic acid was more efficiently (ca. 20 x) converted into oat leaf flavones than was phenylalanine, when the precursor was fed to leaf pieces, and flavones recovered from mesophyll protoplasts. Different labelling patterns were obtained with whole leaf segments and protoplasts which apparently reflect differences in tissue specific flavone biosynthesis o f mesophyll and epidermis. Isolated mesophyll protoplasts incubated with [14C]cinnamic acid synthesize 14C-labelled flavones characteristic o f the mesophyll, as well as several unidentified phenylpropanoid derivatives not found in the intact tissue. Data suggest that photosynthetically active mesophyll cells are a main site o f tissue specific flavone biosynthesis. | | | |
Reference
| Z. Naturforsch. 36c, 222 (1981); received January 141981 | | | |
Published
| 1981 | | | |
Keywords
| Avena sativa, Primary Leaf Tissues, Protoplasts, [14C]Phenylpropanoid Incorporation, C-Glycosyl-flavones | | | |
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| default:Reihe_C/36/ZNC-1981-36c-0222.pdf | | | | Identifier
| ZNC-1981-36c-0222 | | | | Volume
| 36 | |
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