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'Avena sativa L' in keywords Facet   section ZfN Section C  [X]
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1Author    Peter Eilfeld, Wolfhart RüdigerRequires cookie*
 Title    On the Reactivity of Native Phytochrome  
 Abstract    Anilinonaphthalenesulfonate Previous phytochrome models were based on experimental results obtained only with proteo-lytically degraded phytochrome. The reactivity of native phytochrome, in both the Pr and the Pfr form, towards 8-anilinonaphthalene-l-sulfonate (ANS), urea, and ethylene glycol is described here. Whereas native phytochrome in the Pfr form is only very slowly bleached by ANS, immediate bleaching occurs with ANS treated Pr during red irradiation. The bleached form, P£f°, can be converted into Pr660 by prolonged far-red irradiation. A tentative model for native phytochrome is proposed which explains the effects of phototransformatiön, ANS binding and partial proteolysis. 
  Reference    Z. Naturforsch. 39c, 742—745 (1984); received April 10 1984 
  Published    1984 
  Keywords    Avena sativa L, Visible Absorption Spectra, Bleached Phytochrome 
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 TEI-XML for    default:Reihe_C/39/ZNC-1984-39c-0742.pdf 
 Identifier    ZNC-1984-39c-0742 
 Volume    39 
2Author    Fritz Thüm, Thom Ler, W. As Brandlmeier, Olfhart RüdigerRequires cookie*
 Title    Preparation and Properties of Chromopeptides from the Pfr Form of Phytochrome  
 Abstract    Chromopeptides were prepared from the Pfr form o f phytochrome by pepsin digestion. After separation from colorless peptides and Pr peptides by column chromatography, spectral characteristics of this Pfr peptide were determined (A^ax = 610nm, A£ax = 380nm in acid methanol). Irradiation of Pfr peptide produces Pr peptide without liberation of a detectable compound. The Pfr peptide is more sensitive to oxidation and reduction than the Pr peptide. Oxidation with iodine and reduction with dithionite leads to partial chemoconversion o f the Pfr peptide to the Pr peptide. The results favor the model o f cis-trans isomerization for the Pr ^ Pfr transformation. 
  Reference    Z. Naturforsch. 36c, 440 (1981); received February 201981 
  Published    1981 
  Keywords    Avena sativa L, Phytochrome, Pepsin Digestion, Phytochromobilin Peptide, Pfr Peptide 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0440.pdf 
 Identifier    ZNC-1981-36c-0440 
 Volume    36 
3Author    Z. NaturforschRequires cookie*
 Title    A Photoreversible 39 kDalton Fragment from the Pfr Form of 124 kDalton Oat Phytochrome  
 Abstract    Limited proteolysis of 124 kdalton oat phytochrome in the Pfr form is described which leads to a photoreversible 39 kdalton fragment. Whereas the absorption maximum of the Pr form is only slightly shifted (from 667 to 660 nm) no long-wavelength band is observed in the Pfr form. A "bleached" (i.e. low absorbing) form appears instead with a broad absorption maximum at 640 nm. This property corresponds with that of a 40.3 kdalton fragment obtained from pea phytochrome by limited proteolysis (Yamamoto and Furuya, Plant and Cell Physiol. 24, 713, 1983). Dark reversion of the bleached form to the Pr form is faster (f1/2 — 101 min) than dark reversion of 118 kdalton or 60 kdalton Pfr. Low temperature spectroscopy of the 39 kdalton fragment showed that the intermediates lumi-R and meta-Ra are formed like in intact phyto­ chrome or 60 kdalton or 114/118 kdalton fragments. It is discussed that limited proteolysis removed that part of the peptide chain which is responsible for the interaction with the Pfr chromophore but that the site for interaction with the chromophore of Pr and the intermediates lumi-R and meta-Ra is still intact. 
  Reference    Z. Naturforsch. 40c, 693 (1985); received July 5 1985 
  Published    1985 
  Keywords    Avena sativa L, Low Temperature Spectroscopy, Bleached Phytochrome, Dark Reversion 
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 TEI-XML for    default:Reihe_C/40/ZNC-1985-40c-0693.pdf 
 Identifier    ZNC-1985-40c-0693 
 Volume    40 
4Author    PetraH. Eilfeld, Gabriele Widerer, Heike Malinowski, Wolfhart Rüdiger, PeterG. EilfeldRequires cookie*
 Title    Topography of the Phytochrome Molecule as Determined from Chemical Modification of SH-Groups  
 Abstract    124 kDa phytochrome has been isolated from etiolated oat seedlings. Specific chemical modifi-cation of SH-groups has been performed using several agents, especially mercurials. These proce-dures allowed covalent fluorescence labelling of phytochrome. Bleaching of the tetrapyrrole chromophore and reduced photoreversibility has been investigated and analyzed in terms of steric effects, i.e. size of the reagents and number of modified residues. The Pr form was found to exhibit two highly reactive SH-groups, the Pfr form three, however. Two highly reactive groups have been localized on the 55 kDa C-terminal half of the protein. The 3rd group predominantly reacting in the Pfr form has been localized on a 39 kDa chromopeptide, i.e. on the N-terminal half. The covalently attached fluoresceine chromophores were used as reporter groups. The envi-ronment of the fluoresceine chromophore is more hydrophobic in the Pfr form than in the Pr form, indicating that conformational changes take place during photoconversion. 
  Reference    Z. Naturforsch. 43c, 63—73 (1988); received August 31 1987 
  Published    1988 
  Keywords    Oat, Avena sativa L, Mercurials, 124 kDa Phytochrome, Reporter Group 
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 TEI-XML for    default:Reihe_C/43/ZNC-1988-43c-0063.pdf 
 Identifier    ZNC-1988-43c-0063 
 Volume    43 
5Author    Peter Eilfeld, W. Olfhart RüdigerRequires cookie*
 Title    Absorption Spectra of Phytochrome Intermediates  
 Abstract    Native phytochrome (124kdalton) was isolated from etiolated seedlings o f Avena sativa L. cv. Pirol (Baywa, Munich). From low temperature spectra (0 ° C to — 1 6 5 °C) o f phytochrom e samples irradiated either with red or far-red light, spectra o f interm ediates were calculated. Both absorption bands o f the phytochrome chromophore were reported. On the pathway P r -i V r , native phytochrome does not form a bleached interm ediate (m eta-R b) in contrast to degraded phytochrome. An additional intermediate, meta-Rc (/ max = 725 nm), is form ed instead. On the reverse path­ way only two intermediates were found, lum i-F and m eta-F. O scillator strengths' values were calculated and interpreted in terms o f chromophore conform ation. A schem e o f interm ediates for native phytochrome is presented. 
  Reference    Z. Naturforsch. 40c, 109 (1985); received October 8 1984 
  Published    1985 
  Keywords    Avena sativa L, Native Phytochrome, Low Temperature Spectra, Chrom ophore Absorption Bands, Oscillator Strength 
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 TEI-XML for    default:Reihe_C/40/ZNC-1985-40c-0109.pdf 
 Identifier    ZNC-1985-40c-0109 
 Volume    40 
6Author    Verena Scheumann, Michael Helfrich, Siegrid Schoch, Wolfhart RüdigerRequires cookie*
 Title    Reduction of the Formyl Group of Zinc Pheophorbide b in vitro and in vivo: a Model for the Chlorophyll b to a Transformation  
 Abstract    The chemical reduction of the formyl group of pheophorbide b with sodium cyanoborohy­ dride in methanol leads to 7 '-methoxy-and 7 1-hydroxy-pheophorbide a. The same reaction with zinc pheophorbide b yields in addition zinc pheophorbide a. This was characterized by mass and 'H -NM R spectroscopy. Infiltration of zinc pheophorbides a and b and of zinc 7 1-hydroxy-pheophorbide a into etiolated oat leaves yielded phytylated products. The best yield in the esterification was obtained with 7 1-hydroxy-pheophorbide a. Analysis of the products revealed the formation of zinc pheophytin a from all infiltrated compounds. The significance for the transformation of chlorophyll b into chlorophyll a is discussed. 
  Reference    Z. Naturforsch. 51c, 185—1 (1996); received Novem ber 17/D ecem ber 18 1995 
  Published    1996 
  Keywords    Avena sativa L, Infiltration into Etiolated Leaves, Chemical Reduction, Cyanoborohydride, Phytylation 
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 TEI-XML for    default:Reihe_C/51/ZNC-1996-51c-0185.pdf 
 Identifier    ZNC-1996-51c-0185 
 Volume    51