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'Atrazine' in keywords Facet   Publication Year 1981  [X]
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1981[X]
1Author    KatherineE. Steinback, Klaus Pfister, CharlesJ. AmtzenRequires cookie*
 Title    Trypsin-Mediated Removal of Herbicide Binding Sites within the Photosystem II Complex  
 Abstract    Trypsin treatment of isolated chloroplast thylakoids resulted in a step-wise modification o f surface exposed membrane polypeptides. Early effects o f the protease action resulted in a decrease in inhibitory activity of atrazine, diuron, pyrazon, and bromacil, but an initial increase in the activity of bromnitrothymol and dinoseb. Direct measurements o f atrazine binding demonstrated that decreased inhibitory activity corresponded to a decreased binding affinity in the treated membranes. Longer term effects o f trypsin caused removal of atrazine binding sites and a concomitant block o f electron transport chains. The data are consistent with a concept that the traizine receptor protein is a component o f the electron transport chain which is successively degraded in two or more steps by protease attack. Polyacrylamide gel electrophoresis of trypsin-treated membranes and sub-membrane fragments derived from these membranes revealed that several polypeptides are membrane surface exposed. The involvement o f a 32000 dalton polypeptide in creating the atrazine binding site is discussed. 
  Reference    Z. Naturforsch. 36c, 98 (1981); received September 221980 
  Published    1981 
  Keywords    Photosynthesis, Atrazine, Diuron, Chloroplast Membranes, Receptor Protein 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0098.pdf 
 Identifier    ZNC-1981-36c-0098 
 Volume    36