| | 1 | Author
| StefanL. Atza, RalfG B Erger | Requires cookie* | | | Title
| l-0-fra#is-Cinnamoyl-/?-D-glucopyranose: Alcohol Cinnamoyltransferase Activity in Fruits of Cape Gooseberry (Physalis peruviana L.)  | | | | Abstract
| Methyl and ethyl cinnamate are aroma volatiles frequently occurring in fruits. Evidence was obtained that the enzymatic transfer of cinnamic acid to endogenous alcohols present in fruits (methanol, ethanol, 1-propanol) depended on energy-rich 1-O-glycosyl esters of cin namic acid which served as acyl donor molecules. A putative 1-O-fra/w-cinnamoyl-^-D-gluco-pyranose; alcohol cinnamoyltransferase from cape gooseberry (Physalis peruviana L.) was active towards l-0-rra«s-cinnamoyl-/3-D-glucopyranose and l-0-rrans-cinnamoyl-/3-D-gentio-biose. The enzyme was purified 290-fold by a protocol including ammonium sulphate precipi tation, solubilization by Triton X-100, gel permeation and affinity chromatography on conca-navalin A. The acidic glycoprotein {p i = 4.8) most probably is membrane bound. The distribution of alcohol cinnamoyltransferase activity in gel chromatography fractions suggests a native Mr of 75,000. For l-O-mms-cinnamoyl-ß-D-glucopyranose, an apparent K m of 69 ^im was determined. At pH > 6.0, non-enzymatic transesterification superposes the enzymatic transformation. | | | |
Reference
| Z. Naturforsch. 52c, 747—755 (1997); received June 12/July 28 1997 | | | |
Published
| 1997 | | | |
Keywords
| Phenylpropanoid Metabolism, Glycoconjugate, Flavour Precursor, Aroma Ester, Methyl Cinnamate | | | |
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| default:Reihe_C/52/ZNC-1997-52c-0747.pdf | | | | Identifier
| ZNC-1997-52c-0747 | | | | Volume
| 52 | |
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