| | 1 | Author
| RobleyJ. Light, Klaus Hahlbrock | Requires cookie* | | | Title
| Randomization of the Flavonoid A Ring during Biosynthesis of Kaempferol from |l,2 -,3C2j Acetate in Cell Suspension Cultures of Parsley  | | | | Abstract
| Cell suspension cultures o f parsley (Petroselinum hortense) were incubated with [l,2-13C2]ace-tate during a period o f active flavonoid production. The flavonoid glycosides were extracted with ethanol, hydrolyzed in dilute acid, and the aglycones purified by chromatography. Apigenin, a flavone, and kaempferol, a flavonol, were analyzed at 67.9 MHz by 13C FT NMR. The 13C enrich ment confirmed that acetate contributes primarily to the flavonoid A ring. The coupling patterns between adjacent 13C atoms o f the A ring indicate that the cyclization direction of the A ring is random in both compounds. While randomization o f apigenin could have occurred chemically through opening of the pyrone ring under the acid conditions used for glycoside hydrolysis, ran domization o f the more stable flavonol must have occurred biosynthetically. The latter result supports the conclusion that a chalcone is an intermediate in flavonoid biosynthesis. | | | |
Reference
| Z. Naturforsch. 35c, 717—721 (1980); received May 9 1980 | | | |
Published
| 1980 | | | |
Keywords
| 13C-NMR, Apigenin, Kaempferol, Flavonoid, Biosynthesis | | | |
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| default:Reihe_C/35/ZNC-1980-35c-0717.pdf | | | | Identifier
| ZNC-1980-35c-0717 | | | | Volume
| 35 | |
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