| 2 | Author
| G. Forkmann, G. Stotz | Requires cookie* | | Title
| Genetic Control of Flavanone 3-Hydroxylase Activity and Flavonoid 3'-Hydroxylase Activity in Antirrhinum majus (Snapdragon)  | | | Abstract
| In flower extracts o f defined genotypes o f Antirrhinum majus, two different hydroxylases were found catalysing the hydroxylation o f naringenin and eriodictyol in the 3-position and of naringenin in the 3'-position. The 3-hydroxylase is a soluble enzyme and belongs according to its cofactor requirement to the 2-oxoglutarate-dependent dioxygenases. Investigations on different genotypes revealed a clear correlation between block o f the anthocyanin pathway by recessive alleles of the gene inc and a complete lack of 3-hydroxylase activity. Chemogenetic studies on different genotypes suggested that the 3'-hydroxyl group of the B-ring of flavonoids is introduced at the stage of C15 intermediates. The corresponding 3'-hydroxylase was found to be localized in the microsomal fraction and required NADPH as cofactor. In confirmation o f the chemogenetic studies, a strict correlation was found between 3'-hydroxylase activity and the gene eos which is known to control the hydroxylation o f flavones, flavonols and anthocyanins in the 3'-position. These results are similar to those previously obtained with Matthioia incana. | | |
Reference
| Z. Naturforsch. 36c, 411 (1981); received January 20 1981 | | |
Published
| 1981 | | |
Keywords
| Anthocyanin Biosynthesis, Antirrhinum majus, Flavonoids, Flavanone 3-Hydroxylase, Flavonoid 3'-Hydroxylase, Genetic Control | | |
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| default:Reihe_C/36/ZNC-1981-36c-0411.pdf | | | Identifier
| ZNC-1981-36c-0411 | | | Volume
| 36 | |
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