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1980 (1)
1Author    W. Ilhelm Uecker, D.Iethard Kubsch, D. Ieter Lutz, Hans KrögerRequires cookie*
 Title    Einbau von |32P|Orthophosphat in DNA der Mäuse-Milz unter Antigen-Einfluß Incorporation of [32P]O rthophosphate into DN A o f the Mouse Spleen under Influence o f Antigen  
 Abstract    Antigens cause an increase o f the D NA synthesis in the spleen o f mice as shown by the in­ corporation of [32P]orthophosphate and [3H]thym idine. In the present paper, the incorporation of [32P]orthophosphate into the single deoxymononucleotides has been studied under influence of the antigen bovine serum albumin. For this purpose, the labelled D NA was decomposed to the deoxymononucleotides and their specific activities determ ined. Using [32P] orthophosphate, it was found that the activity o f the D NA increased continuously during an observation period of 8 h. While after one hour the relative specific activity o f dTM P was highest and that o f dAMP lowest, the relative specific activities o f the deoxymononucleotides had become equal after 8 h. Under the influence of bovine serum album in, the incorporation o f [32P]orthophosphate increased in the main band DNA as well as in the satellite DNA. The antigen had no effect on the distribution of the specific activities o f the deoxymononucleotides. 
  Reference    Z. Naturforsch. 35c, 599—604 (1980); eingegangen am 24. F ebruar/25. April 1980 
  Published    1980 
  Keywords    Orthophosphate, Mouse Spleen, DNA, Antigen, Desoxymononucleotides 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0599.pdf 
 Identifier    ZNC-1980-35c-0599 
 Volume    35