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1988 (1)
1984 (1)
1Author    Manfred Wiegand, Karl EiseleRequires cookie*
 Title    Isolation and Characterization of the Androgen Receptor of Murine Preputial Gland  
 Abstract    The androgen receptor of murine preputial gland showed in binding experiments a biphasic saturation curve and a biphasic Scatchard plot. The receptor converted from an 8.5-9 S form to a 4.5—5 S form in high ionic strength buffer. The apparent dissociation constant was K D 0.5 ± 0.2 nM for the 8.5—9 S receptor form. A 6.5—7 S receptor form could be detected in some experiments. The ligand specificity was evaluated by competition experiments: testosterone > androstene-dione > dihydrotestosterone > androstanediol > estradiol > progesterone > dexamethasone. The receptor of murine preputial gland was less stable than the androgen receptor of skeletal muscle of the same mice. 
  Reference    Z. Naturforsch. 43c, 117—125 (1988); received September 25 1987 
  Published    1988 
  Keywords    Murine Preputial Gland, Androgen Receptor, Isolation, Characterization 
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 TEI-XML for    default:Reihe_C/43/ZNC-1988-43c-0117.pdf 
 Identifier    ZNC-1988-43c-0117 
 Volume    43 
2Author    Karl Eisele, Fernando Dias Costa, Carlos Pascual, BeatusO. Fenloch-HähnleRequires cookie*
 Title    A Simple Method to Prepare Affinity Resins on Cellulose Basis  
 Abstract    Affinity resins with different spacer arms were synthesized analogues to the solid phase peptide synthesis using aminoethyl cellulose or carboxym ethyl cellulose as matrix. The spacer arms could be varied in length and rigidity. Especially spacer arms consisting o f poly am ino acids can be synthesized with a defined amount o f am ino acid residues specifically in the low molecular weight ranges. The method is also applicable to other matrices w hich are not susceptible to 1 N HC1 in glacial acetic acid, trethylamine, m ethylene chloride and dim ethylform am ide. The synthesis o f affinity resins with different spacer arms for the purification o f androgen receptors is described as an exam ple o f the method. 
  Reference    Z. Naturforsch. 39c, 1048—1051 (1984); received August 8 1984 
  Published    1984 
  Keywords    Affinity Resins, Solid Phase Peptide Synthesis, C ellulose, Androgen Receptor 
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 TEI-XML for    default:Reihe_C/39/ZNC-1984-39c-1048.pdf 
 Identifier    ZNC-1984-39c-1048 
 Volume    39