| | 1 | Author
| Manfred Wiegand, Karl Eisele | Requires cookie* | | | Title
| Isolation and Characterization of the Androgen Receptor of Murine Preputial Gland  | | | | Abstract
| The androgen receptor of murine preputial gland showed in binding experiments a biphasic saturation curve and a biphasic Scatchard plot. The receptor converted from an 8.5-9 S form to a 4.5—5 S form in high ionic strength buffer. The apparent dissociation constant was K D 0.5 ± 0.2 nM for the 8.5—9 S receptor form. A 6.5—7 S receptor form could be detected in some experiments. The ligand specificity was evaluated by competition experiments: testosterone > androstene-dione > dihydrotestosterone > androstanediol > estradiol > progesterone > dexamethasone. The receptor of murine preputial gland was less stable than the androgen receptor of skeletal muscle of the same mice. | | | |
Reference
| Z. Naturforsch. 43c, 117—125 (1988); received September 25 1987 | | | |
Published
| 1988 | | | |
Keywords
| Murine Preputial Gland, Androgen Receptor, Isolation, Characterization | | | |
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| default:Reihe_C/43/ZNC-1988-43c-0117.pdf | | | | Identifier
| ZNC-1988-43c-0117 | | | | Volume
| 43 | |
| 2 | Author
| Karl Eisele, Fernando Dias Costa, Carlos Pascual, BeatusO. Fenloch-Hähnle | Requires cookie* | | | Title
| A Simple Method to Prepare Affinity Resins on Cellulose Basis | | | | Abstract
| Affinity resins with different spacer arms were synthesized analogues to the solid phase peptide synthesis using aminoethyl cellulose or carboxym ethyl cellulose as matrix. The spacer arms could be varied in length and rigidity. Especially spacer arms consisting o f poly am ino acids can be synthesized with a defined amount o f am ino acid residues specifically in the low molecular weight ranges. The method is also applicable to other matrices w hich are not susceptible to 1 N HC1 in glacial acetic acid, trethylamine, m ethylene chloride and dim ethylform am ide. The synthesis o f affinity resins with different spacer arms for the purification o f androgen receptors is described as an exam ple o f the method. | | | |
Reference
| Z. Naturforsch. 39c, 1048—1051 (1984); received August 8 1984 | | | |
Published
| 1984 | | | |
Keywords
| Affinity Resins, Solid Phase Peptide Synthesis, C ellulose, Androgen Receptor | | | |
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| default:Reihe_C/39/ZNC-1984-39c-1048.pdf | | | | Identifier
| ZNC-1984-39c-1048 | | | | Volume
| 39 | |
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