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1985 (1)
1Author    D. Ieter, S. Chubert, K. Arin BossRequires cookie*
 Title    Analytical Ultracentrifugation o f Proteins in Solutions o f Triton X-100 Using Absorption Optics  
 Abstract    Analytical ultracentrifugation studies on proteins in solu­ tions of the nonionic detergent Triton X-100, by means of a UV scanning system, can be conveniently performed by the following procedure: (1) Scanning of the cells is done at a wavelength of 246 nm. (2) The sedimentation of the deter­ gent is prevented by appropriate adjustment of the solvent density. (3) The reference cell is filled with water. Reliable molecular weight determinations can be performed at de­ tergent concentrations up to 0.4% (w/w) in cells of path-length d = 12 mm and at correspondingly higher concen­ trations for d — 3 mm. Approx. 10 ng of protein are suffi­ cient for a determination. The method should be useful for studies of membrane proteins. 
  Reference    Z. Naturforsch. 40c, 908—911 (1985); received June 20/ August 5 1985 
  Published    1985 
  Keywords    Analytical Ultracentrifugation, UV Scanning System Proteins, Triton X-100 
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 TEI-XML for    default:Reihe_C/40/ZNC-1985-40c-0908_n.pdf 
 Identifier    ZNC-1985-40c-0908_n 
 Volume    40