| | 1 | Author
| A. Krauß, A. Hager | Requires cookie* | | | Title
| Wachstums-"burst" nach Anaerobiose: ein "stored grow th"-Effekt beim Streckungswachstum von Koleoptilen Growth Burst after Anaerobiosis: a " Stored-Growth" Effect in Avena Coleoptiles  | | | | Abstract
| Extension growth of 1-cm segments of Avena coleoptiles (studied in liquid medium by means of a flow chamber) was anomalously increased after short anaerobic treatment. The normal growth rate in air-saturated medium without indole-3-acetic acid (IA A) was about 5/«m/min; the rate decreased under anaerobiosis to about 1 ,Mm/min, followed by an increase up to 30 itm/min when aerobic conditions were restored. This "growth burst" continued for about 10 min; then the rate decreased to the initial value of 5 /tm/min. In the same segment growth enhancement could be induced repeatedly by anaerobic treatments. The phenomenon of growth burst after anaerobiosis may be named "anaerobiosis-aerobiosis-transition effect" (ANA-effect). The ANA-effect was also observed under conditions of acid-induced growth at pH 4. Further more it appeared in coleoptile segments whose growth rate had already been enhanced by IA A . Anaerobic pre-treatment not only stimulated extension growth but when applied before IA A addition also overcame the lag phase of IAA-induced growth enhancement. It is supposed that a compound essential for coleoptile growth is accumulated during anaero biosis; such compound together with oxygen may cause the observed growth burst. Possible candi dates for this compound are discussed. | | | |
Reference
| (Z. Naturforsch. 31c, 312 [1976]; eingegangen am 25. Februar 1976) | | | |
Published
| 1976 | | | |
Keywords
| Coleoptiles, Growth Enhancement, Auxin Effect, Anaerobiosis | | | |
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| default:Reihe_C/31/ZNC-1976-31c-0312.pdf | | | | Identifier
| ZNC-1976-31c-0312 | | | | Volume
| 31 | |
| 2 | Author
| Reiner Merz, Friedh Schneider | Requires cookie* | | | Title
| Growth Characteristics of in vitro Cultured Ehrlich Ascites Tumor Cells under Anaerobic Conditions and after Reaeration | | | | Abstract
| The effects of deprivation of oxygen on proliferation kinetics of in vitro grown Ehrlich ascites tumor cells were studied by means of flow cytometry. The flow cytometric results obtained by applying the BUdR-H 33 258 technique showed that 6 -8 hours after establishment of anaerobiosis cells lose their capacity for proliferation and accumulate in the late G1-phase; during this time late S cells enter the G2 compartment but do not divide while cells, which are in G2+M at the beginning of anaerobiosis divide and enter G l. Cell cycle distribution remained constant up to about 20 hours, thereafter Gl-cells enter the S-phase. Up to 24 hours of anaerobiosis cell number does not change and viability of the cells was not severely affected. Recultivation under aerobic conditions of 12 hours anaerobically treated cells revealed, that the cytokinetic properties of Gl cells were not impaired, while S-phase cells after reaeration did not enter G2 but began again to synthesize DNA forming cells with a DNA content greater than 4c. S-phase cells are most sensitive to deprivation of oxygen. | | | |
Reference
| Z. Naturforsch. 37c, 326—334 (1982); received December 71981 | | | |
Published
| 1982 | | | |
Keywords
| Ehrlich Ascites Tumor Cells, Anaerobiosis, Growth Characteristics | | | |
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| default:Reihe_C/37/ZNC-1982-37c-0326.pdf | | | | Identifier
| ZNC-1982-37c-0326 | | | | Volume
| 37 | |
| 5 | Author
| Rekha Chaturvedi, M. Singh, P. V. Sane | Requires cookie* | | | Title
| Photoinactivation of Photosynthetic Electron Transport under Anaerobic and Aerobic Conditions in Isolated Thylakoids of Spinach | | | | Abstract
| The effect o f exposure to strong white light on photosynthetic electron transport reactions o f PS I and PS II were investigated in spinach thylakoids in the absence or presence o f oxygen. Irrespective o f the conditions used for photoinactivation, the damage to PS II was always much more than to PS I. Photoinactivation was severe under anaerobic conditions compared to that in air for the same duration. This shows that the presence o f oxygen is required for prevention o f photoinactivation o f thylakoids. The susceptibility o f water-splitting com plex in photoinactivation is indicated by our data from experiments with chloride-deficient chloro-plast membranes wherein it was observed that the whole chain electron transport from DPC to MV was much less photoinhibited than that from water. The data from the photoinactivation experiments with the Tris-treated thylakoids indicate another photodam age site at or near reaction centre o f PS II. D CM U -protected PS II and oxygen-evolving com plex from p h oto inactivation. D C M U protection can also be interpreted in terms o f the stability o f the PS II complex when it is in S2 state. | | | |
Reference
| Z. Naturforsch. 47c, 63—6 (1992); received August 16 1991 | | | |
Published
| 1992 | | | |
Keywords
| Photoinactivation, Anaerobiosis, Inhibitors, Photosynthetic Electron Transport, Trypsinization | | | |
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| default:Reihe_C/47/ZNC-1992-47c-0063.pdf | | | | Identifier
| ZNC-1992-47c-0063 | | | | Volume
| 47 | |
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