| | 2 | Author
| Ulrike Strohmeier, ChristianG. Erdes3, Wolfgang Lockaub | Requires cookie* | | | Title
| Proteolysis in Heterocyst-Forming Cyanobacteria: Characterization of a Further Enzyme with Trypsin-Like Specificity, and of a Prolyl Endopeptidase from Anabaena variabilis  | | | | Abstract
| of the cyanobacterium Anabaena variabilis ATCC 29413 and an en gineered mutant that lacks an intracellular protease cleaving after Lys and Arg (Maldener, Lockau, Cai, and Wolk, Mol. Gen. Genet. 225, 113-120 (1991)) were separated by ion ex change chromatography, and protease profiles determined using azocasein, Na-benzoyl-D,L-arginine-4-nitroanilide and N-carbobenzoxy-glycyl-L-proline-4-nitroanilide as substrates. A second enzyme cleaving at the carboxyl side of lysine and arginine, and a prolyl endopepti dase were detected, enriched and characterized. Both proteolytic enzymes appear to be located in the periplasm. | | | |
Reference
| Z. Naturforsch. 49c, 70—78 (1994); received September 29/December 81993 | | | |
Published
| 1994 | | | |
Keywords
| Anabaena variabilis, Cyanobacterium, Heterocysts, Proteolytic Enzymes, Prolyl Endopeptidase Soluble extracts | | | |
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| default:Reihe_C/49/ZNC-1994-49c-0070.pdf | | | | Identifier
| ZNC-1994-49c-0070 | | | | Volume
| 49 | |
| 3 | Author
| ShigetohM. Iyachi11, JoachimB. Ürger, K. Iriakos, K. Otzabasisb, JensT. Hielm Annc, H. O. Rst Senger | Requires cookie* | | | Title
| Photosynthetic Characteristics of Three Strains of Cyanobacteria Grown under Low-or High-C02 Conditions | | | | Abstract
| Quantum requirements of photosynthetic oxygen evolution at 679 nm, fluorescence em is sion spectra at liquid nitrogen temperature (77 K) and fluorescence induction kinetics in the presence of DCM U, were measured in the cyanobacteria Anabaena variabilis M3, Anabaena variabilis ATCC 29413 and A nacystis nidulans R2, each grown under low-or high-C02 condi tions. L o w -C 0 2 grown cells o f the cyanobacteria showed a higher quantum requirement of photosynthetic oxygen evolution and a higher ratio o f F7U)_740 to F680_700 fluorescence and a lower variable fluorescence in the presence of D CM U than high-C02 grown cells. These findings indicate a change in excitation energy distribution in favour of photosystem I. The result might be an enhancement in ATP formation caused by cyclic electron flow which in turn provokes dissolved inorganic carbon (D IC) accumulation in these low-C02 grown cells. | | | |
Reference
| Z. Naturforsch. 51c, 40—4 (1996); received August 4/October 6 1995 | | | |
Published
| 1996 | | | |
Keywords
| Quantum Requirement, Fluorescence, Dissolved Inorganic Carbon, Anabaena variabilis, Anacystis nidulans | | | |
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| default:Reihe_C/51/ZNC-1996-51c-0040.pdf | | | | Identifier
| ZNC-1996-51c-0040 | | | | Volume
| 51 | |
| 5 | Author
| Gottfried Martin, Peter Böger | Requires cookie* | | | Title
| Two Ways of Hydrogen Peroxide Formation in the Oxidative Inactivation of Cyanobacterial Glutamine Synthetase | | | | Abstract
| Using crude extracts from the cyanobacterium Anabaena variabilis glutamine synthetase (GS) activity was rapidly irreversibly reduced to about 60% during dark incubation ("sponta neous GS inactivation"). An additional decrease was observed by the addition of ammonia in the light ("ammonia-m ediated inactivation"). Both effects were prevented by EDTA, MnCl2 or catalase indicative of the involvement of H 20 2. This is a key interm ediate in oxida tive enzyme inactivation. In both spontaneous and am monia-mediated GS inactivation H 20 2 is produced in different ways. Spontaneous inactivation is prevented by depletion of reduced pyridine nucleotides which apparently donate electrons to produce H 20 2. Fractionation of the crude extract showed that the light-enhanced GS inactivation by ammonia required the presence of thylakoid membranes. The photosynthesis inhibitor DCM U decreased GS inacti vation by ammonia. For the inactivation in the light apparently H 20 2 is produced from super oxide during photosynthetic electron transport. | | | |
Reference
| Z. Naturforsch. 52c, 812—816 (1997); received August 12 1997 | | | |
Published
| 1997 | | | |
Keywords
| Glutamine Synthetase, Protein Oxidation Ammonia Effect, Photosynthetic Hydrogen Peroxide Production, Cyanobacterium, Anabaena variabilis | | | |
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| default:Reihe_C/52/ZNC-1997-52c-0812.pdf | | | | Identifier
| ZNC-1997-52c-0812 | | | | Volume
| 52 | |
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