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'Ammonium Assimilation' in keywords Facet   Publication Year 1981  [X]
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1Author    Kassem Alef, Hans-Joachim Burkardt, Hans-Joachim Horstmann, WalterG. ZumftRequires cookie*
 Title    Molecular Characterization of Glutamine Synthetase from the Nitrogen-Fixing Phototrophic Bacterium Rhodopseudomonas palustris1  
 Abstract    The phototrophic bacterium Rhodopseudomonas palustris assimilated ammonium via glutamine synthetase and glutamate synthase. Diazotrophic and ammonium-grown cells had high levels of both enzymes, whereas enzymes o f alternative assimilatory pathways were absent or had only low activities. Glutamine synthetase was purified to electrophoretic homogeneity within three steps by dye-ligand and ion exchange chromatography. Electron microscopy revealed a dodecameric molecular entity which was in accordance with parameters derived from electrophoretic techniques. The molecular weight of the enzyme monomer was 55800; that o f the dodecamer 670000. The amino acid composition o f R. palustris glutamine synthetase was determined and compared by a statistical method with other known enzyme compositions from prokaryotic and eukaryotic origins. 
  Reference    Z. Naturforsch. 36c, 246—254 (1981); received December 231980 
  Published    1981 
  Keywords    Rhodopseudomonas palustris, Glutamine Synthetase, Dye-Ligand Chromatography, Nitrogen Fixation, Ammonium Assimilation 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0246.pdf 
 Identifier    ZNC-1981-36c-0246 
 Volume    36