| | 1 | Author
| Christoph Giersch | Requires cookie* | | | Title
| Photophosphorylation by Chloroplasts: Effects o f Low Concentrations o f Ammonia and Methylamine  | | | | Abstract
| Intact chloroplasts exposed to hypotonic assay conditions are capable o f photophosphorylating exogenous ADP. The rate o f phosphorylation by these unbroken plastids is increased by 10—50% upon the addition o f low concentrations (< mM) o f N H 3 or C H 3 N H 2. Stimulation o f phosphory lation is abolished by washing chloroplasts with MgCl2. Evidence is presented that washing re moves a factor responsible for amine-induced increase o f A T P production and that this factor is associated with the thylakoid membrane. Addition o f CH3 N H 2 increased the proton permeability of the thylakoid membrane o f unbroken and washed chloroplasts during the light/dark transition. Hence, differences o f the membrane permeability for protons between the two preparations seem not to be responsible for an increase o f A T P production upon the addition o f amines. Stimulation of photophosphorylation by methylamine is observed even at light itensities which do not saturate the proton motive force, which in turn is reduced upon the addition o f the uncoupler. Apparently, phosphorylation can be stimulated, although the limiting driving force is diminished. It is con cluded that phosphorylation by unbroken chloroplasts under low light illumination is limited kinetically, not energetically. Consequences o f these findings for observation made with intact chloroplasts are discussed. | | | |
Reference
| Z. Naturforsch. 37c, 242—250 (1982); received December 23 1981 | | | |
Published
| 1982 | | | |
Keywords
| Photophosphorylation, Uncoupling, Amines, Proton Motive Force, Chloroplasts, Chemiosmotic Theory | | | |
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| default:Reihe_C/37/ZNC-1982-37c-0242.pdf | | | | Identifier
| ZNC-1982-37c-0242 | | | | Volume
| 37 | |
| 2 | Author
| G. Lyn, R. Banks, A. Lan, J. Buglass, S. W. Aterhouse | Requires cookie* | | | Title
| Amines in the Marking Fluid and Anal Sac Secretion of the Tiger, Panthera tigris | | | | Abstract
| Analysis of the marking fluid of two tigers (one Bengal and one Sumatran) by GC using an amine-specific column and a nitrogen-specific detector has shown the presence of the follow ing amines: ammonia, methylamine, dimethylamine, trimethylamine, triethylamine, propyl amine, and butane-1,4-diamine (putrescine). In contrast to previously published reports, we were unable to detect 2-phenylethylamine. The anal sac secretion was found to have a similar amine content. | | | |
Reference
| Z. Naturforsch. 47c, 618—620 (1992); received March 12 1992 | | | |
Published
| 1992 | | | |
Keywords
| Amines, Anal Sac Secretion, Marking Fluid, Scent-Marking, Tiger | | | |
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| default:Reihe_C/47/ZNC-1992-47c-0618.pdf | | | | Identifier
| ZNC-1992-47c-0618 | | | | Volume
| 47 | |
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