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1991 (1)
1Author    GabrieleG. Ünther, Henrik LaaschRequires cookie*
 Title    Local Anesthetic Binding to Thylakoid Membranes. Relation to Inhibition of Light-Induced Membrane Energization and Photophosphorylation  
 Abstract    The association o f the lipophilic tertiary amine and local anesthetic dibucaine with osm oti-cally shocked chloroplasts o f Spinacia oleracea L. cv. M onatol was investigated. Dibucaine, known as an effective inhibitor o f thylakoid membrane energization and A TP synthesis, ex­ hibited three distinct binding classes with chloroplasts: partitioning in the lipid phase o f the membranes, electrostatic screening o f negative electrical charges on the thylakoid surface and light-induced association o f an as yet unknown nature. Evidence is presented that the mecha­ nism o f inhibition o f the transthylakoid pH gradient, ApH, by dibucaine is distinct from 'clas­ sical' amine-type uncoupling: The inhibitory effect o f dibucaine on ApH was independent o f the initial strength o f ApH. Light-induced dibucaine binding was independent o f the volum e o f the intrathylakoid space and o f the strength o f ApH as varied by medium pH. Judged from a comparison o f the data on dibucaine binding and on inhibition o f ApH and photophosphory­ lation, dibucaine bound via partitioning in the membrane lipid phase is responsible for the un-coupler-like effects o f the local anesthetic. A mechanism for the inhibition o f thylakoid energi­ zation by local anesthetic amines is discussed. 
  Reference    Z. Naturforsch. 46c, 79—8 (1991); received September 21 1990 
  Published    1991 
  Keywords    Chloroplast, Local Anesthetic, Dibucaine, Uncoupling, Photophosphorylation, Amine Bind­ ing 
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 TEI-XML for    default:Reihe_C/46/ZNC-1991-46c-0079.pdf 
 Identifier    ZNC-1991-46c-0079 
 Volume    46