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1979 (2)
1Author    Ruth Seeger, Tjakko StijveRequires cookie*
 Title    Amanitin Content and Toxicity of Amanita verna Bull  
 Abstract    The < x-, /?-, and ^-amanitin content of 11 samples of Amanita verna Bull., collected during 1975 — 1978 in Germany and Switzerland, has been determined by high performance thin-layer chromatography (HPTLC) of crude methanolic extracts. The toxicity (i. v. LD50 for mice of defatted, lyophilized, methanolic extracts) of 3 samples has been compared with that of A. phal­ loides from the same site of collection. The amanitin content of A. verna ranged from 2250 to 4570 mg/kg dry weight; the fungi con­ tained almost as much ß-as a-amanitin, whereas the y-amanitin content amounted to about 12% of the total amanitin. A. verna contained less amanitin (65% on the average) than A. phalloides from the same collection site, but it was not significantly less toxic, since the phallotoxins contributed to the toxicity of either species in our tests. 
  Reference    Z. Naturforsch. 34c, 330 (1979); received February 5 1979 
  Published    1979 
  Keywords    Amanitins, Amanita verna, Amanita phalloides, Mushroom Poisoning, Chromatography 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0330.pdf 
 Identifier    ZNC-1979-34c-0330 
 Volume    34 
2Author    Tjakko Stijve, Ruth SeegerRequires cookie*
 Title    Determination of a-, ß and y-Amanitin by High Performance Thin-Layer Chromatography in Amanita phalloides (Vaill. ex Fr.) Seer, from Various Origin  
 Abstract    A fast, sensitive high performance thin-layer chrom atographic method for the determ ination of a-, ß-, and y-amanitin in crude, m ethanolic extracts o f Amanita phalloides is described. The limit o f detection is 50 ng of each amanitin. W ith this method am anitin was determ ined in 24 pooled samples of Amanita phalloides, collect­ ed between 1970 and 1977 in G erm any and Switzerland. The total am anitin content varied be­ tween 2010 and 7300 mg/kg dry weight and the average value was 4430 m g/kg of which 43% was a-am anitin, 49% /?-amanitin and 8% y-amanitin. The origin of the fungi hardly influenced their am anitin content: in samples collected during the same year at different sites it fluctuated within a factor o f 1.7. The am anitin content of samples from the same site, but collected in different years, maximally varied within a factor o f 3.7. The partial decom position of am anitins during prolonged storage of the lyophilized samples undoubtedly contributed to this variation. Phalloidin, which was determined by conventional thin-layer-chrom atography, could not be de­ tected in a sample from 1970, whereas its concentration in m aterial collected during 1977 am ount­ ed to 2400 mg/kg dry weight. The toxicity of the samples (LD 50 o f lyophilized defatted methanolic extracts intravenously for mice) varied within a factor of 2.5. 
  Reference    Z. Naturforsch. 34c, 1133—1138 (1979); received July 9 1979 
  Published    1979 
  Keywords    Amanita phalloides, Amanitins, Phalloidin, M ushroom Poisoning, Chrom atography 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-1133.pdf 
 Identifier    ZNC-1979-34c-1133 
 Volume    34