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1980 (1)
1Author    Regina Teschner, Stefan Postius, M. Onika Löffler, Friedhelm SchneiderRequires cookie*
 Title    Cell Proliferation and Metabolism of Ehrlich Ascites Tumor Cells Grown in Chemically Defined Albumin Medium for Several Passages  
 Abstract    Cell proliferation, cell cycle distribution, viability, uptake o f ar-aminoisobutytic acid, cyclo leucine and 2-deoxyglucose as well as DNA, RNA and protein synthesis of Ehrlich ascites tumor cells grown in suspension culture over three passages in serum free nutrient medium supplemented with 1 -2 % bovine serum albumin (Cohn fraction V, Serva) were measured and compared to cells grown in medium supplemented with 15% horse serum. At the end of the fourth passage in vitro, that is the third passage in serum free albumin medium, the growth o f the cells is reduced to about 1 0 % of the controls, accumulation of the cells in G 2 is delayed and the number o f dead cells rises to 30%. The transport rates o f a-aminoisobutyric acid and o f 2-deoxyglucose slowly decrease while the up­ take of cycloleucine shows a more complex behaviour in albumin medium. Incorporation o f thy­ midine and leucine into the cells corresponds to the growth rate, while the incorporation o f uridi­ ne in normal as well as in albumin medium exhibits different pattern. The present experiments demonstrate, that it may be possible to preserve this cells in a fairly "physiological" state during two passages in albumin medium. 
  Reference    Z. Naturforsch. 35c, 117 (1980); received October 29 1979 
  Published    1980 
  Keywords    Albumin Medium, Cell Growth, Ehrlich Ascites Tumor Cells, Metabolism 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0117.pdf 
 Identifier    ZNC-1980-35c-0117 
 Volume    35