| | 1 | Author
| G. Kulandaivelu, D. 0. Hall | Requires cookie* | | | Title
| Stabilization of the Photosynthetic Activities of Isolated Spinach Chloroplasts during Prolonged Ageing  | | | | Abstract
| Isolated spinach chloroplasts (type A complete) were used to study the changes in the pnoto-chemical activities upon storage in order to establish optimum conditions for prolonged storage. Chloroplasts stored at — 5 °C were found to retain over 70% of their photosynthetic electron transport from H20 even after 5 days. Increases in the level of state 2 electron transport (due to uncoupling) with concomitant loss of state 3 phosphorylation activity was observed during the initial period of ageing. Addition of 1% bovine serum albumin decreased the level of uncoupling and maintained the phosphorylation activity for a longer period. Chloroplasts stored at 77 °K main tained their phosphorylation capacity for a period of 10 days even after repeated freezing and thawing. Photosystem I activity was found to be more stable over the whole ageing period of 15 days. Loss of plastoquinone may be responsible for the decrease in electron transport between photosystems II and I. | | | |
Reference
| (Z. Naturforsch. 31c, 452 [1976]; received August 22 1975/March 11 1976) | | | |
Published
| 1976 | | | |
Keywords
| Ageing, Stabilization, Photosynthetic Activities, Chloroplasts | | | |
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| default:Reihe_C/31/ZNC-1976-31c-0452.pdf | | | | Identifier
| ZNC-1976-31c-0452 | | | | Volume
| 31 | |
| 2 | Author
| G. K. Ulandaivelu An, D.0 H All | Requires cookie* | | | Title
| Ultrastructural Changes in in vitro Ageing Spinach Chloroplasts | | | | Abstract
| Ultrastructural changes in in vitro ageing spinach chloroplasts have been studied in detail. Prolonged storage caused swelling of the chloroplasts due to the increase in the thickness and spacing of the thylakoid membranes. The increase in the thickness of the membrane is partly ac companied by the release of lipids. Addition of crystalline bovine serum albumin was found to stabilize the membrane structures. Storage of the chloroplasts at 77 °K even though it resulted in complete breakage of the whole chloroplasts, maintained the thylakoid structures in a highly intact form. | | | |
Reference
| (Z. Naturforsch. 31c, 82 [1976]; received August 8 1975) | | | |
Published
| 1976 | | | |
Keywords
| Ultrastructure, Thylakoid Membrane, Ageing, Storage Conditions, Spinach | | | |
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| default:Reihe_C/31/ZNC-1976-31c-0082.pdf | | | | Identifier
| ZNC-1976-31c-0082 | | | | Volume
| 31 | |
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