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'Adriamycin' in keywords Facet   section ZfN Section C:Volume 039  [X]
Facet   Publication Year 1984  [X]
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1984[X]
1Author    EvaP. Aur, RichardJ Y Oungm An, E. Dm, L. En, G. Feld Er, ErichF E LstnerRequires cookie*
 Title    Mechanisms of Adriamycin-Dependent Oxygen Activation Catalyzed by NADPH-Cytochrome c*-(Ferredoxin)-Oxidoreductase  
 Abstract    Under aerobic conditions, 0 2 uptake and production o f 0 2~ and H 20 2 by isolated N A D P H -cytochrome c-(ferredoxin)-oxidoreductase from Euglena gracilis was strongly stim ulated by adriamycin. Further stim ulation was not observed with 0.1 m M F e 3+-EDTA. M ethionine fragmentation (measured as ethylene release), as a reliable indicator for the formation o f O H ' radical-like oxidants under aerobic conditions (1 0 0 |im o l 0 2 in a 10 ml reaction vessel) was strongly stimulated by 0.1 m M F e3+-ED TA or, in the absence o f iron, by partial anaerobiosis (1 (irnol 0 2 per vessel). The highest rate o f m ethionine fragm entation was observed under an­ aerobic conditions in the presence o f both reduced adriam ycin and added H 20 2. Aerobic methionine fragmentation in the presence o f adriam ycin and F e3+-ED TA was inhibited by superoxide dismutase and catalase by m ore than 90%, w hile m ethionine fragmen­ tation under semianaerobiosis in the absence o f F e 3+-E D T A was inhibited by superoxide dismutase to only about 50%, while catalase again inhibited by m ore than 90%. These results indicate that the adriamycin-catalyzed production o f a strong oxidant appears to be governed by different mechanisms depending on oxygen availability; nam ely the production o f a Fenton-type oxidant driven by adriamycin-catalyzed superoxide form ation and also, the formation o f the "crypto-OH' radical" by direct electron donation from the adriam ycin sem iquinone radical to H20 2 under oxygen limiting conditions. 
  Reference    Z. Naturforsch. 39c, 261—267 (1984); received D ecem ber 15 1983 
  Published    1984 
  Keywords    Adriamycin, Oxygen Activation, Oxygen Toxicity, Superoxide, H ydroxyl Radical 
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 Identifier    ZNC-1984-39c-0261 
 Volume    39