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'Adenosine Deaminase' in keywords
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1983 (1)
1979 (1)
1Author    Jacek Wierzchowski, David ShugarRequires cookie*
 Title    Sensitive Fluorimetric Assay for Adenosine Deaminase with Formycin as Substrate; and Substrate and Inhibitor Properties of some Pyrazolopyrimidine and Related Analogues  
 Abstract    The nucleoside antibiotic formycin, 7-amino-3-(/?-D-ribofuranosyl)pyrazolo(4,3-d)pyrimidine, a structural analogue of adenosine, is deaminated about 10-fold faster by adenosine deaminase than adenosine itself, and is therefore a superior substrate for both routine assays and kinetic studies with the purified enzyme. The luminescence properties o f formycin have been profited from to develop a fluorimetric assay for adenosine deaminase which is considerably more sensi­ tive than the spectrophotometric procedure widely employed with adenosine as substrate. Exam­ ples are presented of its application to routine assays of adenosine deaminase levels in cellular extracts, as well as to kinetic studies with the purified enzyme, including the properties o f some pyrazolopyrimidine and purine substrates and inhibitors. 
  Reference    Z. Naturforsch. 38c, 67—7 (1983); received August 23 1982 
  Published    1983 
  Keywords    Formycin, Pyrazolopyrimidines, Adenosine Deaminase, Fluorimetric Assay, Inhibitors 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0067.pdf 
 Identifier    ZNC-1983-38c-0067 
 Volume    38 
2Author    Frank Seela, Johann Ott, Helmut RosemeyerRequires cookie*
 Title    Immobilisierung von Adenosinacetalen mit variablem Alkylidenrest — die Funktion des Spacers bei enzymatischer Desaminierung Immobilization of Adenosine-Acetals with Variable Alkylidene Residues — the Function of the Spacer during Enzymatic Deamination —  
 Abstract    Acetalation of the cis-diol moiety of adenosine or inosine with aliphatic ketoesters of different chain lengths leads to alkylidene derivatives of the nucleosides, which differ in the number of methylene groups in the hydrocarbon chain. By alkaline hydrolysis of the ester group in l a — c or 3 a —c the corresponding acids 2 a —c and 4 a —c have been prepared. The configuration of the new chiral centres has been determined as R. Enzymatic deamination of the alkylidene derivatives of adenosine leads to the inosine compounds. The rate of deamination reaction is raised by an increasing number of methylene groups in the alkylidene residues or by use of the esters instead of the acids. The alkylidene derivatives of adenosine were coupled with 6-aminohexylagarose yielding polymers with adenosine as ligands. No enzymatic deamination of the polymer with the shortest spacer was observed. The polymers with the longer spacers were converted to the corresponding inosine derivatives. The velocity of the deamination reaction was raised by an increasing spacer length. 
  Reference    Z. Naturforsch. 34c, 350—358 (1979); eingegangen am 30. 12. 1978/12. 2. 1979 
  Published    1979 
  Keywords    Cyclic 0-2', 3'-Adenosine-acetals, Affinity Resins, Spacer, Adenosine Deaminase 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0350.pdf 
 Identifier    ZNC-1979-34c-0350 
 Volume    34