| | 1 | Author
| Anders Ljungqvist, Karl Folkers+ | Requires cookie* | | | Title
| Synthesis of a Combined Fragment of Interferon, AeHuIFN a A(30-43)-(123-137)-NH2 Interferon May not be a Sychnologieal Peptide * AeHuIFN a A( 30-43)-( 123-137)-NH2, Ac-Leu-Lys-Asp-Arg-His-Asp-Phe-Gly-Phe- Pro-Gln-Glu-Glu-Phe-Phe-Gln-Arg-Ile-Thr-Leu-Tyr-Leu-Lys-Glu-Lys-Lys-Tyr-Ser- Pro-NH2 was  | | | | Abstract
| synthesized toward encompassing the "active site" of the interferons. The design of this 29-amino acid peptide was based on considerations of homology between the different interferons and on combining two regions of interferons, and on known and predicted structural features. The peptide was characterized by amino acid analysis, thin layer chromatography in several systems, and by HPLC. As tested, the peptide neither showed antiviral activity nor blocked antiviral activity of interferon, indicating that the active site was not encompassed or that interferon may not be a sychnologieal peptide. | | | |
Reference
| Z. Naturforsch. 38b, 1249—1252 (1983); received June 24 1983 | | | |
Published
| 1983 | | | |
Keywords
| Interferon, Peptide Synthesis, Antiviral Activity, Synthetic Fragment, Active Site | | | |
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| default:Reihe_B/38/ZNB-1983-38b-1249.pdf | | | | Identifier
| ZNB-1983-38b-1249 | | | | Volume
| 38 | |
| 3 | Author
| DhirendraL. Nandi | Requires cookie* | | | Title
| | | | | Abstract
| The 14C labelled inactive protein obtained by sodium borohydride reduction of the enzyme, porphobilinogen syn thase of R h od op se u d om on a s sp h eroid es, in the presence of [4 -u C ] 5-aminolevulinic acid, gave on acid hydrolysis and subsequent electrophoresis or two-dimensional chromato graphy a m ajor radioactive spot which was confirmed to be N -f-[4-(-5am inovaleric acid) ] lysine (A L A -ly s in e) by comparing its co-chromatographic and electrophoretic be haviour with the chemically synthesized A L A -lysin e. A n e -N H , group of lysine residue of porphobilinogen synthase, is thus the binding site of the substrate, 5-aminolevulinic acid. | | | |
Reference
| Z. Naturforsch. 33c, 799 (1978); received A p ril 25/June 6 1978 | | | |
Published
| 1978 | | | |
Keywords
| Borohydride Reduction, L abelled Protein, Hydrolysis Lysine, Active Site | | | |
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| default:Reihe_C/33/ZNC-1978-33c-0799_n.pdf | | | | Identifier
| ZNC-1978-33c-0799_n | | | | Volume
| 33 | |
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