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1983 (1)
1Author    Enrico Boccù, Roberta Largajolli, FrancescoM. VeroneseRequires cookie*
 Title    Coupling of Monomethoxypolyethyleneglycols to Proteins via Active Esters  
 Abstract    Two alternative methods for the attachment of monomethoxypolyethyleneglycols (PEG) to proteins are proposed; they are based upon the replacement of the hydroxy terminal function of PEG to carboxylate followed by its activation with dicyclohexylcarbodiimide and N-hydroxy-succinimide. The methods, which give more homogeneous product than that employing trichloro-s-triazine as coupling reagent, may also be used for the modification of essential — SH containing enzymes. The attachment of PEG activated via esters was tested with several model proteins and the influence of the extent o f modification i. on the biological activity o f various enzymes, ii. on the binding capacity for albumin and iii. on the clearance time in rats using superoxide dismutase as model tracer was evaluated. It was also demonstrated that the extent of PEG attachment varies greatly according to the different proteins used. 
  Reference    Z. Naturforsch. 38c, 94 (1983); received July 7/September 29 1982 
  Published    1983 
  Keywords    Protein Surface Modification, Monomethoxypolyethyleneglycols Activation, PEG, Enzyme Therapy, Active Esters 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0094.pdf 
 Identifier    ZNC-1983-38c-0094 
 Volume    38