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1988[X]
1Author    Bernhard HuchzermeyerRequires cookie*
 Title    Phosphate Binding to Isolated Chloroplast Coupling Factor (CF^  
 Abstract    A single binding site for phosphate was found on isolated chloroplast coupling factor in the absence of nucleotides. In our experiments the phosphate binding site showed a K d of 170 JIM. We did not observe any differences whether the ATPase activity of CF] had been activated or not. If the enzyme was incubated with [y-32 P]ATP the amount of 32 P bound per CF, depended on the pretreatment of the enzyme: In the presence of ADP no ATP or phosphate was bound to CF,. After activation of ATPase activity one mol of ATP per mol CF, was rapidly bound and hydro-lyzed while there was a slowly occurring binding of another phosphate without concomitant nucleotide binding. We conclude that there are two different types of phosphate binding observed in our experi-ments: 1) Inorganic phosphate can be bound by one catalytic site per mol of CTv 2) The y-phosphate of ATP is able to bind to an ATP binding domain of the enzyme if this domain can exchange substrates with the incubation medium. This ATP binding domain appears to differ from the site binding inorganic phosphate, because at least a portion of the coupling factor contains more than one labelled phosphate during our ATPase tests. 
  Reference    Z. Naturforsch. 43c, 213—218 (1988); received July 27/November 19 1987 
  Published    1988 
  Keywords    Chloroplast, Coupling Factor, Binding Sites, ATPase, Phosphorylation 
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 TEI-XML for    default:Reihe_C/43/ZNC-1988-43c-0213.pdf 
 Identifier    ZNC-1988-43c-0213 
 Volume    43