Go toArchive
Browse byFacets
Bookbag ( 0 )
'A m ino Acids' in keywords
Results  2 Items
Sorted by   
Section
Publication Year
1996 (1)
1979 (1)
1Author    Florenz Sasse, Dietlinde Backs-Hüsemann, Wolfgang BarzRequires cookie*
 Title    Isolation and Characterization of Vacuoles from Cell Suspension Cultures of Daucus carota  
 Abstract    A reliable procedure for the isolation o f vacuoles from anthocyanin-containing cells o f Daucus carota cell suspension cultures has been developed. From cells o f the late linear growth phase, protoplasts were prepared and purified in a sucrose/sorbitol gradient. Vacuoles were liberated from these protoplasts by osm otic shock and purified in a M etrizamide step gradient. The vacuole-containing fractions were analysed for their anthocyanin content as a measure for the yield o f vac­ uoles. The purity o f vacuoles was examined by assaying various marker enzym es in both proto­ plasts and vacuoles. The purest vacuolar fraction had 8% o f the total activity o f glucose-6-phos-phate dehydrogenase (marker for cytosol), 8% o f cytochrom e oxidase (m itochondria) and 10% o f N A DPH -cytochrom e c reductase (ER). 55% o f the acid phosphatase activity o f the protoplasts and 35% o f the total malate were recovered in the vacuoles. The vacuolar pool o f am ino acids is quite large. D ata for 15 am ino acids show that 44 to 73% are being located in the vacuole. 
  Reference    Z. Naturforsch. 34c, 848—853 (1979); received M ay 15 1979 
  Published    1979 
  Keywords    Daucus carota, Protoplasts, Vacuoles, Lysosomes, A m ino Acids, Anthocyanin 
  Similar Items    Find
 DEBUG INFO      
 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0848.pdf 
 Identifier    ZNC-1979-34c-0848 
 Volume    34 
2Author    M.C M Arcucci, F. A. De, C. Am Argo, C.M A LopesRequires cookie*
 Title    Identification of Amino Acids in Brazilian Propolis  
 Abstract    A m ino acids o f propolis samples from different origins were analysed and quantified. Seven of them presented almost the same percentage in all samples, indicating that bees may have obtained amino acids from three sources: plants, pollen contamination and from their own metabolism. The regeneration properties of propolis in relation to the presence of som e amino acids are discussed. 
  Reference    Z. Naturforsch. 51c, 11 (1996); received March 27/O ctober 12 1995 
  Published    1996 
  Keywords    Propolis, A m ino Acids, Pollen, Plant Sources, Regenerative Properties 
  Similar Items    Find
 DEBUG INFO      
 TEI-XML for    default:Reihe_C/51/ZNC-1996-51c-0011.pdf 
 Identifier    ZNC-1996-51c-0011 
 Volume    51