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'6 Type Cytochrome' in keywords Facet   section ZfN Section C:Volume 037  [X]
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1982 (1)
1Author    Hendrik Hüdig, Gerhart DrewsRequires cookie*
 Title    Isolation of a b-Type Cytochrome Oxidase from Membranes of the Phototrophic Bacterium Rhodopseudomonas capsulata  
 Abstract    A cytochrome oxidase (EC 1.9.3.1) was solubilized from the membrane fraction o f aerobically grown cells of Rhodopseudomonas capsulata by treatment with Triton X-100. The enzyme was purified 160 fold by chromatography on DEAE-Sepharose CL -6B and affinity chromatography on cytochrome c-thiol activated Sepharose 4B. The purified enzyme has a pH-optimum at 8.5 and a temperature optimum at 35 °C. The ap­ parent K m for reduced horse cytochrome c is 24 |iM (at pH 8 and 30 °C). The purified cytochrome oxidase was 50% inhibited by 1.5 |iM KCN and 10 (iM N aN ,. The purified enzyme contained one polypeptide of mT 65,000 and 6-type cytochrome. 
  Reference    Z. Naturforsch. 37c, 193—198 (1982); received November 2 December 1 1981 
  Published    1982 
  Keywords    Rhodopseudomonas capsulata, Purification, Solubilization, Cytochrome c, Cytochrome Oxidase, 6-Type Cytochrome 
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 TEI-XML for    default:Reihe_C/37/ZNC-1982-37c-0193.pdf 
 Identifier    ZNC-1982-37c-0193 
 Volume    37