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'3 O G lucosyltransferase' in keywords
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1984 (1)
1Author    L.M V Jonsson, M.E G Aarsman, J. Bastiaannet, W.E D Onker-K, A.G M Oopm An, A. W. Geräts, SchramRequires cookie*
 Title    Common Identity of UDP-Glucose: Anthocyanidin 3-O-Glucosyltransferase and UDP-Glucose: Flavonol 3-O-Glucosyltransferase in Flowers of Petunia hybrida  
 Abstract    In an attempt to distinguish between the U D P -glucose: flavonol 3-O -glucosyltransferase (3G T) and the UD P-glucose:anthocyanidin 3-O-glucosyltransferase in flower buds o f Petunia hybrida, several properties o f these activities were determ ined. The 3-glucosylation o f anthocyanidin had a pH-activity optimum o f 7.2, that o f flavonol pH 9.2 to 9.5. A nthocyanidin 3 G T activity was lowered in the presence o f EDTA or /?-mercaptoethanol, but this was due to an effect on the anthocyanidin substrate. The two 3-glucosylating activities were to a sim ilar extent inhibited by an increasing ionic strength in the enzyme assay and showed an identical iso-electric point (5.2) as determined by chromatofocusing. M olecular weights were identical: 26000, 52000 or 78000 daltons as determined by gel-filtration. Antiserum raised against partially purified 3 G T gave identical immunoprecipitation curves with flavonol 3 G T and anthocyanidin 3G T . Special attention was given to the 3-O-glucosyltransferase in mutants with low levels o f 3 G T activity. These mutants are unable to form significant am ounts o f anthocyanins but contain wild-type amounts o f flavonols. The enzyme o f such mutants had the sam e iso-electric point and identical titration-curves with antiserum as the enzym e from w ildtype plants. 3 G T from w ildtype or mutant plants glucosylated flavonols at higher rates than anthocyanidins. 
  Reference    Z. Naturforsch. 39c, 559 (1984); received February 21 1984 
  Published    1984 
  Keywords    Petunia hybrida, Anthocyanins Flavonoid Biosynthesis, Flavonols, 3-O -G lucosyltransferase 
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 TEI-XML for    default:Reihe_C/39/ZNC-1984-39c-0559.pdf 
 Identifier    ZNC-1984-39c-0559 
 Volume    39