| | 1 | Author
| PetraH. Eilfeld, Gabriele Widerer, Heike Malinowski, Wolfhart Rüdiger, PeterG. Eilfeld | Requires cookie* | | | Title
| Topography of the Phytochrome Molecule as Determined from Chemical Modification of SH-Groups  | | | | Abstract
| 124 kDa phytochrome has been isolated from etiolated oat seedlings. Specific chemical modifi-cation of SH-groups has been performed using several agents, especially mercurials. These proce-dures allowed covalent fluorescence labelling of phytochrome. Bleaching of the tetrapyrrole chromophore and reduced photoreversibility has been investigated and analyzed in terms of steric effects, i.e. size of the reagents and number of modified residues. The Pr form was found to exhibit two highly reactive SH-groups, the Pfr form three, however. Two highly reactive groups have been localized on the 55 kDa C-terminal half of the protein. The 3rd group predominantly reacting in the Pfr form has been localized on a 39 kDa chromopeptide, i.e. on the N-terminal half. The covalently attached fluoresceine chromophores were used as reporter groups. The envi-ronment of the fluoresceine chromophore is more hydrophobic in the Pfr form than in the Pr form, indicating that conformational changes take place during photoconversion. | | | |
Reference
| Z. Naturforsch. 43c, 63—73 (1988); received August 31 1987 | | | |
Published
| 1988 | | | |
Keywords
| Oat, Avena sativa L, Mercurials, 124 kDa Phytochrome, Reporter Group | | | |
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| default:Reihe_C/43/ZNC-1988-43c-0063.pdf | | | | Identifier
| ZNC-1988-43c-0063 | | | | Volume
| 43 | |
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