| | 1 | Author
| Virginia Massheimer, LuisM. Fernandez, AnaR. De Boland | Requires cookie* | | | Title
| Stimulation of Calmodulin Binding to Skeletal Muscle Membrane Proteins by 1,25-Dihydroxy-Vitamin D 3  | | | | Abstract
| Previous work has shown that 1,25-dihydroxy-vitamin D 3 rapidly increases calmodulin lev els of skeletal muscle membranes without altering the muscle cell calmodulin content. There fore, the effects of the sterol on the binding of calmodulin to specific muscle membrane pro teins were investigated. Soleus muscles from vitamin D-deficient chicks were treated in vitro for short intervals (5-15 min) with physiological concentrations of 1,25-dihydroxy-vitamin D 3. Proteins of mitochondria and microsomes isolated by differential centrifugation were sep arated on sodium dodecyl sulfate polyacrylamide gels. Calmodulin-binding proteins were identified by a [125I]calmodulin gel overlay procedure followed by autoradiography. 1,25-Di-hydroxy-vitamin D 3 increased the binding of labelled calmodulin to a major, calcium-inde pendent, calmodulin-binding protein of 28 Kda localized in microsomes, and to minor calmo dulin-binding proteins of 78 and 130 Kda proteins localized in mitochondria. The binding of [125I]calmodulin to these proteins was abolished by flufenazine or excess non-radioactive cal modulin. 1,25-Dihydroxy-vitamin D 3 rapidly increased muscle tissue Ca uptake and cyclic AM P levels and stimulated the phosphorylation of several membrane proteins including those whose calmodulin-binding capacity potentiates. Analogously to the sterol, forskolin increased membrane calmodulin content, calmodulin binding to the 28 Kda microsomal protein and 45Ca uptake by soleus muscle preparations. Forskolin also induced a similar profile of changes in muscle membrane protein phosphorylation as the hormone. These results suggest that 1,25-dihydroxy-vitamin D 3 affects calmodulin distribution in muscle cells through cyclic AMP-de-pendent phosphorylation of membrane calmodulin-binding proteins. These changes may play a role in the stimulation of muscle Ca uptake by the sterol. | | | |
Reference
| Z. Naturforsch. 45c, 663—670 (1990); received October 2/November 3 1989 | | | |
Published
| 1990 | | | |
Keywords
| 1, 25-Dihydroxy-Vitamin D 3, Skeletal Muscle, Muscle Membranes, Calmodulin Binding, Protein Phosphorylation | | | |
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| default:Reihe_C/45/ZNC-1990-45c-0663.pdf | | | | Identifier
| ZNC-1990-45c-0663 | | | | Volume
| 45 | |
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