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1981 (1)
1Author    Fumio Yoshii, Isao Kaetsu, Akio YamadaRequires cookie*
 Title    Immobilization of Erythrocytes by Radiation Polymerization of Glass-Forming Monomers at Low Temperatures  
 Abstract    The immobilization of erythrocyte as the whole cell without hemolysis was studied. It found that erythrocyte could be treated and immobilized stably by radiation polymerization o f specific monomers having high viscous and long oxyethylene units chain such as methoxypolyethylene-glycol methacrylate (M-23G) and polyethyleneglycol dimethacrylate (14G). Irradiation dose without hemolysis was limited less than lxl05r and a comonomer system consisting o f M -23G-14G, 1:1 and small quantity o f glutaraldehyde (GA) was the optimum carrier composition. The functional properties o f the immobilized erythrocyte was also investigated. It was found that the immobilized cell could be carried out carbon monoxide-oxygen gas exchange effectively and reversibly so as in the intact cell. The immobilized erythrocyte also showed the catalase activity just as in the intact cell. The stability o f erythrocyte increased greatly by the immobilization for standing at low and room temperatures and hardly hemolyzed in non-isotonic medium such as pure water. It was observed in scanning electron microscope that the immobilized erythrocyte had a hollow disk shape same as in intact cell and covered with a thin polymer layer. 
  Reference    Z. Naturforsch. 36c, 1062—1067 (1981); received June 2 1981 
  Published    1981 
  Keywords    Erythrocyte, Radiation Polymerization, Immobilization, Glass-Forming Monomer, Low Temperature, 0 2-Uptake 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-1062.pdf 
 Identifier    ZNC-1981-36c-1062 
 Volume    36