| | 1 | Author
| St Blech, H. Budzikiewicz, D. Dill | Requires cookie* | | | Title
| /?-Carboline Alkaloids from R ibes nigrum L  | | | | Abstract
| From Ribes nigrum L. two ß-carboline alkaloids (deshydroxymethyl flazin (1) and flazin methyl ether (2)) could be isolated in minute amounts. The structure elucidation is described. The two compounds are present in the freshly pressed juice, but they are also formed during storage of the juice by condensation of tryptophane with ascorbic acid or its degradation products. This could be shown by adding 14C-labelled ascorbic acid to the juice. A fter four weeks' incubation radioactive 1 and 2 could be isolated. | | | |
Reference
| Z. Naturforsch. 49c, 540 (1994); received May 20 1994 | | | |
Published
| 1994 | | | |
Keywords
| Ribes nigrum L, ß-Carboline Alkaloids, Flazin Derivatives, Biogenesis | | | |
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| default:Reihe_C/49/ZNC-1994-49c-0540.pdf | | | | Identifier
| ZNC-1994-49c-0540 | | | | Volume
| 49 | |
| 2 | Author
| J. Berlinab, I. N. Kuzovkinac, C. Rügenhagenab, L. Fecker3, U. C. Om, M. Andeur3, V. W. Rayb | Requires cookie* | | | Title
| Hairy Root Cultures of Peganum harmala II. Characterization of Cell Lines and Effect of Culture Conditions on the Accumulation of ß-Carboline Alkaloids and Serotonin | | | | Abstract
| Hairy root cultures o f Peganum harmala established by genetic transformation with A gro bacterium rhizogenes wild type strains A 4, 15834 or carrying the binary vector pLTCgus 1, re spectively, were analyzed with respect to their tryptophan-derived m etabolites. Beside the pre viously detected ß-carboline alkaloids harmine, harmol, harmaline and harmalol all hairy root cultures were found to contain the ß-carboline glucoside ruine and serotonin. The appearance o f the root cultures and the levels o f ß-carboline alkaloids were greatly affected by the nitrogen source o f the medium. In the presence o f higher levels o f ammonium ions (as in MS medium) the roots were short and thick, while in B50 medium (low ammonium) they were thin and long. Ammonium ions had a negative effect on the levels o f ß-carbolines. The best root cul tures accumulated on B50 1 .5 -2 % ß-carbolines on a dry mass basis and in the presence o f 6% sucrose ca. 10 mg ß-carbolines/L x d were formed. In the presence o f phytohorm ones root cul tures were converted into low producing suspension cultures, from which the highly produc tive roots were reinduced on phytohormone-free medium. | | | |
Reference
| Z. Naturforsch. 47c, 222 (1992); received July 29/Novem ber 11 1991 | | | |
Published
| 1992 | | | |
Keywords
| Peganum harmala, R oot Cultures, Transformation, ß-Carboline Alkaloids, Serotonin | | | |
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| default:Reihe_C/47/ZNC-1992-47c-0222.pdf | | | | Identifier
| ZNC-1992-47c-0222 | | | | Volume
| 47 | |
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