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Facet section ZfN Section C:Volume 035 [X]

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101	Author	KlausJ N Eurohr, HenryH. Mantsch	Requires cookie*
	Title	Complex Formation of Carnosine with Purine Nucleotides in Aqueous Solution
	Abstract	This ^ -N M R study provides experimental evidence for an intermolecular interaction between the dipeptide carnosine (yS-alanyl-L-histidine) and the purine nucleoside 5'-m onophosphates 5'-AM P, 5'-IMP and 5'-GM P. From the observed upfield shifts o f the purine nucleotide and im idazole proton resonances it is concluded that the interaction is o f the stacking type and that it involves the purine base o f the nucleotide and the histidine m oiety o f carnosine. Apparent microscopic equilibrium constants and com plex shifts are obtained with a m icroscopic m odel, which considers the formation o f both 1:1 and 1 :2 complexes. The stacking pattern for com plex formation between the histidine m oiety o f carnosine and the adenine m oiety o f 5'-AM P is con structed by fitting the experimental 5'-AM P com plex for histidine.
	Reference	Z. Naturforsch. 35c, 557 (1980); received D ecem ber 10 1979
	Published	1980
	Keywords	Carnosine, Purine N ucleotides, Proton N M R, Base Stacking, Equilibrium Constants
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	TEI-XML for	default:Reihe_C/35/ZNC-1980-35c-0557.pdf
	Identifier	ZNC-1980-35c-0557
	Volume	35

102	Author	W. G. Pohl, R. K. Reikenbohm, K. Seuwen	Requires cookie*
	Title	The Specificity of Ionophore A 23187 in Cation Transport across Lipid Membranes Studies with Lecithin Vesicles
	Abstract	Lecithin vesicles containing different cations were prepared by sonication and characterized. When the concentration o f the divalent cations in the buffer was increased from 1 m M to about 100 m M the cation-concentration inside o f the vesicles did not increase proportional to the outside concentration, but showed a saturation behaviour. The efflux o f various cations m ediated by ionophore A 23187 was m easured and relative transport-rates were determined. The following sequence was obtained: Zn2+ > Mn2+ > Ca2+ > M g2+ > Sr2+ > Ba2+ % L i+ > N a +. The efflux o f Ca2+ increased proportional to the square o f the A 2 3 187-concentration. To lecithin-vesicles containing ethylenediam ine tetraacetate (ED TA) inside different cations were added on the outside. In the presence o f A 23187 divalent cations are transported into the vesicles and bound there by EDTA. During the influx o f M e2+-ions into vesicles a H +-efflux was observed. The resulting pH-decrease was measured. The rate o f the pH -change depended on the M e2+-ion used. The sequence was: Cd2+ > Zn2+ > Ca2+ > M n2+ > M g2+ > Sr2+, Ba2+.
	Reference	Z. Naturforsch. 35c, 562 (1980); received February 11/M arch 6 1980
	Published	1980
	Keywords	Ionophore A 23187, Lecithin Vesicles, Cation Specificity, Proton Antiport
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	TEI-XML for	default:Reihe_C/35/ZNC-1980-35c-0562.pdf
	Identifier	ZNC-1980-35c-0562
	Volume	35

103	Author	Helm Ut Sapper, Wolfgang Gohl, Wolfgang Lohmann	Requires cookie*
	Title	31P-und ^-NMR-Untersuchungen über die Wechselwirkung von ATP mit Mg2+, Ca2+ und Monoaminen 31P-and 'H-NM R-Investigations about the Interaction of ATP with Mg2+, C a2+, and Monoamines
	Abstract	The interaction between aromatic amines and ATP is based upon an electrostatic attraction o f the protonated am ino groups to the negatively charged phosphate groups o f ATP com bined with a stacking association between the ring systems. The com plexes formed are relatively weak (as sociation constants 4 -4 8 M_1), shortlived (life tim e smaller than 0.02 s), and m ainly 1:1 in stoichiometry preferring the terminal phosphate o f ATP as the site o f association. The divalent metal ions M g2+ and Ca2+ bond stronger than the am ines (association constants 1500 and 100 M_1, resp.) prefer the /?-phosphate group changing thereby the geom etry o f the ATP phosphate chain. Thus, they reduce the am ine-ATP association in ternary mixtures.
	Reference	Z. Naturforsch. 35c, 569—577 (1980); eingegangen am 27. März 1980
	Published	1980
	Keywords	ATP, Phosphate Interaction, Stacking Association, Phosphorus and Proton M agnetic Resonance
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	TEI-XML for	default:Reihe_C/35/ZNC-1980-35c-0569.pdf
	Identifier	ZNC-1980-35c-0569
	Volume	35

104	Author	Yoriko Taketo, Akira Taketo	Requires cookie*
	Title	Effects of Polynucleotides on Production and Activity of Streptolysin S
	Abstract	Activity o f various natural and synthetic polynucleotides as a carrier for streptolysin S was tested in a resting cell system. As the carrier, intact m olecules o f M S2 R N A , E. coli tR N A or rat liver R N A were almost inactive, whereas R N ase I core o f these R N A s, especially the core frac tions eluted from DEA E-cellulose colum n at higher NaC l concentrations, effectively induced pro duction o f the extracellular hem olysin. The carrier activity o f yeast R N A was significantly en hanced by sim ultaneous addition o f R N ase I, to the streptococcal suspension. Preincubation o f yeast R N A with growing streptococci or a protein fraction from the bacterial culture supernatant increased its carrier activity. Evidences were obtained suggesting involvem ent o f streptococcal nuclease in the enhancement o f the carrier effect. Production o f the streptococcal hem olysin was markedly prom oted by polyguanylic acid but neither by polyadenylic acid, polycytidylic acid nor by polyuridylic acid. Like trypan blue, polyguanylic acid exerted potent inhibitory effect on hem olytic activity o f streptolysin S complex. Similar but less marked effect was observed with certain R N ase I core fractions o f rat liver R N A. Som e data concerning effect o f polynucleotides on stability o f the hem olysin were also presented.
	Reference	Z. Naturforsch. 35c, 578 (1980); received March 10 1980
	Published	1980
	Keywords	Streptolysin S, R N A, Polyguanylic Acid, Streptococcus pyogenes
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	TEI-XML for	default:Reihe_C/35/ZNC-1980-35c-0578.pdf
	Identifier	ZNC-1980-35c-0578
	Volume	35

105	Author	Jürgen Pauluhn, Annem Arie, N. Aujok, H. Erbert, W. Zim, Erm Ann	Requires cookie*
	Title	Über die quantitative fluorometrische Bestimmung von DNA mit Ethidiumbromid in der Zelle und die Konstanz der Quantenausbeute des Ethidium-DNA-Komplexes im biologischen Milieu On the Q uantitative Fluorom etric D eterm ination of Cellular DNA by Ethidium Bromide and the Constancy o f the Quantum Yield of the Ethidium -D N A -Com plex in the Biological Environment
	Abstract	The fluorescent staining o f fixed A 9 cells (m ouse fibroblasts) with ethidium brom ide E has been investigated qualitatively and quantitatively. E is bound to D N A , R N A and protein. After enzymatic R N A digestion and staining at pH = 4.0 the dyestuff is bound to D N A specifically. The formed E -D N A -com plex is stoichiom etric if D N A will be saturated by high dye concentra tion, CF = 1.0 x 10~2 M. The stoichiom etric factor n = 0.21 was determined from Scatchard bind ing isotherms and by spectrophotometric titration. The binding o f E to D N A and R N A has been investigated by binding isotherms. E is bound to D N A by intercalation and by ionic interaction. It is bound to R N A by ionic interaction only. The ionically bound dyestuff can be replaced by salts like LiCl from D N A and RNA. Only inter calated E remains. The com petitive salt effect can be used to avoid the enzym atic R N A digestion in the specific staining o f D N A . W ithin the cell the E-D N A -com plex fluoresces strongly. U sing a microspectrophotom eter we determined the fluorescence intensity J and the extinction E in cell sections o f q = 1 \|a2 area. The fluorescence was excited in the m inimum o f the absorbance o f the E -D N A -com plex at = 365 nm. The extinction E 2 was measured in the short wavelength band at X2 = 260 nm. Under these conditions we received a linear relation J = J (E ?) up to high extinctions E 2. The quantum yield Q o f the E -D N A -com plex is nearly constant and independent o f the biological environment. Q is also unaltered by LiCl. W ithin the lim its o f error intercalated and ionic bound dye have the same quantum yield. Under the conditions o f stoichiom etric staining the am ount o f E -D N A -complex and therefore D N A in the cell can be determined by J. The extinction coefficient e2 = 45200 M-1 cm -1 o f the E -D N A -com plex at 260 nm was calcu lated from the concentration dependence o f the absorbance spectra using the law o f mass action. By e2 the am ount o f D N A in the cell is accessible from J. According to our measurements with A 9 cells the superficial density o f D N A has the order o f m agnitude £>n = m ^ /q = 101 nmol cm -2, the amount raN o f D N A per section q = 1 jj.2, mN = 10_l fmol, and the D N A concentration in the cell nucleus CN = 10-1 M. The ray pass and diagram o f electronics o f the used m icrospectrophotom eter are described.
	Reference	Z. Naturforsch. 35c, 585—5 (1980); eingegangen am 14. Januar/21. April 1980
	Published	1980
	Keywords	D N A -determ ination, Ethidium Bromide, M ouse Fibroblasts, Specific Fluorescent Staining, Quantum Y ield, M icrospectrophotometer
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	TEI-XML for	default:Reihe_C/35/ZNC-1980-35c-0585.pdf
	Identifier	ZNC-1980-35c-0585
	Volume	35

106	Author	W. Ilhelm Uecker, D.Iethard Kubsch, D. Ieter Lutz, Hans Kröger	Requires cookie*
	Title	Einbau von \|32P\|Orthophosphat in DNA der Mäuse-Milz unter Antigen-Einfluß Incorporation of [32P]O rthophosphate into DN A o f the Mouse Spleen under Influence o f Antigen
	Abstract	Antigens cause an increase o f the D NA synthesis in the spleen o f mice as shown by the in corporation of [32P]orthophosphate and [3H]thym idine. In the present paper, the incorporation of [32P]orthophosphate into the single deoxymononucleotides has been studied under influence of the antigen bovine serum albumin. For this purpose, the labelled D NA was decomposed to the deoxymononucleotides and their specific activities determ ined. Using [32P] orthophosphate, it was found that the activity o f the D NA increased continuously during an observation period of 8 h. While after one hour the relative specific activity o f dTM P was highest and that o f dAMP lowest, the relative specific activities o f the deoxymononucleotides had become equal after 8 h. Under the influence of bovine serum album in, the incorporation o f [32P]orthophosphate increased in the main band DNA as well as in the satellite DNA. The antigen had no effect on the distribution of the specific activities o f the deoxymononucleotides.
	Reference	Z. Naturforsch. 35c, 599—604 (1980); eingegangen am 24. F ebruar/25. April 1980
	Published	1980
	Keywords	Orthophosphate, Mouse Spleen, DNA, Antigen, Desoxymononucleotides
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	TEI-XML for	default:Reihe_C/35/ZNC-1980-35c-0599.pdf
	Identifier	ZNC-1980-35c-0599
	Volume	35

107	Author	LaurenG. Otsuki, D. Ieter, W. Gruenwedel	Requires cookie*
	Title	Methylmercury-Chromosome Interactions. I. Thermal Denaturation of Calf Thymus Chromatin in Presence of CH3HgOH
	Reference	Z. Naturforsch. 35c, 605 (1980)
	Published	1980
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	Identifier	ZNC-1980-35c-0605
	Volume	35

108	Author	ThomasL J Boehm, Dusan Drahovsky	Requires cookie*
	Title	Die Beziehung der enzymatischen Methylierung invers repetitiver DNA Sequenzen zur Transkription in Maus P815 Mastocytoma Zellen The Relation o f Enzymatic M ethylation of Inverted DNA Repeats to Transcription in Mouse P815 M astocytoma Cells
	Abstract	The isolation of transcribed D NA sequences of P815 cells and the partial characterization with respect to their sequence composition and relative rates o f enzymatic D NA m ethylation are reported in this paper. Transcribed regions were purified by affinity chrom atography using im mobilized heterogenous nuclear RNA of P815 cells. About 10% of total genome was found in this fraction. Reassociation analyses showed differences in sequence composition o f transcribed versus non-transcribed D N A fractions. The relative proportion of inverted repeats was doubled in the transcribed fraction whereas ordinary highly repetitive sequences comprising mainly o f satellite D NA were found alm ost exclusively in the non-transcribed regions o f the P815 genome. A bout 70% of transcribed portions corresponds to unique and interm ediary D NA sequences. A fter labelling of cells with L-[Methyl-3H]methionine and [14C]deoxycytidine relative rates o f enzymatic DNA m ethylation were computed for different kinetic components o f transcribed and non-transcribed portions of P815 genome. No difference was found except in inverted repeats. In transcribed D NA the relative rate o f enzymatic DNA methylation was only about 40% o f that of the non-transcribed ones. We have quantitated this hypomethylation and found that there is, in average, about one 5-methylcytosine residue in 100 nucleotides o f transcribed inverted repeats, com pared to about 2.5 5-methylcytosines in non-transcribed fractions. In view of these data we propose that the enzymatic methylation o f inverted DNA repeats negatively controls the tran scriptional process in a given genomic region.
	Reference	Z. Naturforsch. 35c, 611—6 (1980); eingegangen am 30. Januar 1980
	Published	1980
	Keywords	Enzymatic Methylation, Inverted D NA Repeats, Transcriptional Control
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	TEI-XML for	default:Reihe_C/35/ZNC-1980-35c-0611.pdf
	Identifier	ZNC-1980-35c-0611
	Volume	35

109	Author	H. Schnabl	Requires cookie*
	Title	Anion Metabolism as Correlated with the Volume Changes of Guard Cell Protoplasts
	Abstract	Isolated and purified guard cell protoplasts o f starch-containing (Vicia faba) and starch-lacking (Allium cepa) stom ata were used to study the m etabolism o f m alate and C l-anions as correlated with the K +-induced swelling o f protoplasts. The correlation between swelling and malate synthesis in starch-containing and the inhibition of this correlation in starch-lacking guard cell protoplasts were demonstrated. A model system was proposed involving the role o f m alate and its place of synthesis in these two contrasting stomatal types.
	Reference	Z. Naturforsch. 35c, 621 (1980); received March 27 1980
	Published	1980
	Keywords	Allium cepa, G uard Cell Protoplasts, Malate, Vicia faba
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	TEI-XML for	default:Reihe_C/35/ZNC-1980-35c-0621.pdf
	Identifier	ZNC-1980-35c-0621
	Volume	35

110	Author	Aloysius Wild, Barbara Urschel	Requires cookie*
	Title	Chlorophyll-Protein Complexes of Chlorella fusca
	Abstract	Chlorophyll-protein complexes from thylakoids of the normal type and two mutants o f Chlorella fusca were separated using sodium dodecyl sulfate acrylamide gel electrophoresis (PAGE). The properties o f the chlorophyll-protein complexes o f the three strains of Chlorella were compared. Standard curves were set up for the characterization of the chlorophyll-proteins. In every electrophoretic separation of chlorophyll-protein complexes, a certain am ount o f pigm ent is separated from the protein. We tried to keep that am ount as low as possible by mild solubiliza tion and by working in low tem perature. U nder these conditions, we obtained several new chlorophyll-proteins in addition to the P-700-chlorophyll a-protein (CP I) and the light-harvesting chlorophyll a/b-protein (CP II). Thus, a small band (CP I a) was located between the top of the gel and the CP I after elec trophoresis. A lthough it shows spectral qualities sim ilar to CP I, it possesses a much lower chloro phyll a/chlorophyll b ratio. It may be an aggregate of photosystem I and light-harvesting chloro phyll. We found and analyzed three other chlorophyll-proteins with electrophoretic m obilities inter mediate between that o f the P-700-chlorophyll a-protein and that of the light-harvesting chloro phyll a/b-protein complex. Two o f these chlorophyll-proteins, the LHCP1 and the L H C P2, have a low chlorophyll a/chlorophyll b ratio and spectral properties similar to that o f the light-harvesting chlorophyll a/b-protein (LH CP3). They obviously represent dimers or oligomers o f the latter com plex. A third, new complex (CPa) m igrated between LHCP3 and its dimer. W ith the chlorophyll b deficient m utant G 36 of Chlorella fusca, this complex could be obtained in high purity and great enrichment (15% of total chlorophyll). It could be proved that this complex only contains chloro phyll a. Its red absorption m axim um is at 671 nm. Some indirect evidences suggest that it may be a good candidate for the PS II reaction center complex.
	Reference	Z. Naturforsch. 35c, 627—6 (1980); received March 19 1980
	Published	1980
	Keywords	Chlorella, C hlorophyll-Protein Complexes, Polyacrylam ide-Gel Electrophoresis, Standard Curves for Characterization o f Chlorophyll Proteins
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	Identifier	ZNC-1980-35c-0627
	Volume	35

111	Author	G.Erhard Vierke	Requires cookie*
	Title	Kinetics of the Back Reaction of Photosystem II as Derived from Delayed Light Emission in the Presence of DCMU and the Conformational State of the Thylakoid Membrane
	Abstract	of the Thylakoid Membrane It is shown that the kinetics of the back reaction o f photosystem II in the seconds time range as derived from the luminescence decay curve in the presence of DCM U is controlled by the internal pH of the thylakoids. M odifications o f the conformational state of the photosynthetic m em brane while leaving the internal pH unchanged, however, reaction.
	Reference	Z. Naturforsch. 35c, 638 (1980); received January 28/M ay 5 1980
	Published	1980
	Keywords	Photosynthesis, Photosystem II, Delayed Light Emission, Conform ational State
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	Identifier	ZNC-1980-35c-0638
	Volume	35

112	Author	K.H G Rum Bach, B. Fom	Requires cookie*
	Title	Chloroplast Autonomy in Acetyl-Coenzyme-A-Formation and Terpenoid Biosynthesis
	Abstract	Isolated intact spinach chloroplasts were supplied with 14C-labeled C 0 2, phosphoglycerate, phos-phoenolpyruvate, acetate and mevalonate and the incorporation o f radioactivity into /?-carotene and plastoquinone-9 assayed. All applied substrates were capable of crossing the chloroplast envelope. Except phosphoenolpyruvate all radioactive precursors were incorporated into ß-carotene and also into plastoquinone-9. It is concluded that spinach chloroplasts are autonom in acetyl-coenzyme-A-formation and terpenoid biosynthesis. There is a direct carbon flow from intermediates of the Calvin-cycle via acetyl-coenzyme-A and mevalonic acid existing in the chlo roplast leading to the carotenoids and isoprenoid side-chains o f chlorophylls and quinones.
	Reference	Z. Naturforsch. 35c, 645—648 (1980); received March 25 1980
	Published	1980
	Keywords	Biosynthesis, /?-Carotene, Chloroplast, Plastoquinone-9, Terpenoids
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	TEI-XML for	default:Reihe_C/35/ZNC-1980-35c-0645.pdf
	Identifier	ZNC-1980-35c-0645
	Volume	35

113	Author	GeoffreyA. Codd, G. Eorg, H. Schmid	Requires cookie*
	Title	Effects of S-^^-DichlorophenyO-N-N'-Dimethylurea on Oxygen Evolution and Fluorescence by Whole Filaments and Isolated Thylakoids of the Cyanobacterium Anabaena cylindrica
	Abstract	The site of inhibition of DCM U against photosystem II in the cyanobacterium (Blue-green alga) Anabaena cylindrica was examined in electron transport and fluorescence studies. Isolated thylakoids catalyzed silicomolybdate photoreduction using H zO as electron donor; the steady-state reaction was completely inhibited by DCM U. This reaction is insensitive to DCM U in chloro plasts, since silicomolybdate accepts electrons from the prim ary photosystem II acceptor, and thus before the site of action o f D CM U in higher plants. DCM U did not increase the steady-state level of fluorescence by inact A. cylindrica, nor affect the monophasic fluorescence induction, with
	Reference	Z. Naturforsch. 35c, 649—655 (1980); received April 18 1980
	Published	1980
	Keywords	Oxygen-Evolving Side, Cyanobacteria, D CM U-Sensitivity, Fluorescence
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	Identifier	ZNC-1980-35c-0649
	Volume	35

114	Author	D. Meier, H. K. Lichtenthaler, G. Burkard	Requires cookie*
	Title	Change of Chloroplast Ultrastructure in Radish Seedlings under the Influence of the Photosystem II-Herbicide Bentazon
	Abstract	The influence of the photosystem II-herbicide bentazon on the ultrastructure o f chloroplasts o f radish seedlings (Raphanus sativus L.) was investigated with special emphasis on thylakoid developm ent and grana formation. Bentazon application (10-3 m) induces the formation of broader and higher grana stacks (grana width: 0 .5 -0 .6 n m ; greatest frequency: 3 -8 thylakoids per granum) than in the control plants (grana width: 0.3 \|im; greatest frequency: 2 -4 thylakoids per granum). Furtherm ore, the am ount of chloroplast lamellae is enhanced, as are the stacking degree of thylakoids and the grana area. The chloroplasts of bentazon-treated plants appear to be shorter and thicker than in the controls and show all signs of a shade-type adaptation of the photosynthetic apparatus.
	Reference	Z. Naturforsch. 35c, 656 (1980); received March 17 1980
	Published	1980
	Keywords	Bentazon, Chloroplast-Ultrastructure, G rana Form ation, Photosystem II-Herbicide, Shade-type Chloroplasts
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	Identifier	ZNC-1980-35c-0656
	Volume	35

115	Author	PedroE H Aeser, G. Ottfried, W. Erner	Requires cookie*
	Title	Verminderter Sauerstoffverbrauch und reduzierte Atemfrequenz verursacht durch "Schweres Wasser" (D zO) bei Fischen Reduced Oxygen Consumption and Reduced O perculare Movements in Fishes by Heavy W ater (D 20 )
	Abstract	Operculare M ovements in Fishes, Oxygen Consumption, Heavy Water (D 20) Fishes (Scardinius erythrophthalmus) have a almost 40% reduced oxygen consum ption and a 18% reduced operculare movements in normal water containing 20% "heavy water" (D 20) .
	Reference	Z. Naturforsch. 35c, 665 (1980); eingegangen am 24. Februar/10. April 1980
	Published	1980
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	Identifier	ZNC-1980-35c-0665
	Volume	35

116	Author	Helga Sittertz-Bhatkar	Requires cookie*
	Title	Oral Cleansing in Spiders is Gland Mediated!
	Abstract	Histochemical and micromorphological investigations of the gnathocoxal gland in spiders brought out its uniquely adaptive cleansing function. The prey particulates, caught in the oral mi crostructures, are bound with mucopolysaccharides or glyco-muco-proteins from the glandular se cretions in Araneus and Theridion species into a bolus, and are expelled following each food up take.
	Reference	Z. Naturforsch. 35c, 669—673 (1980); received October 20 1979
	Published	1980
	Keywords	Oral Cleansing, Spiders, Gnathocoxal Gland, Histochemistry, Micromorphological Investigations
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	Identifier	ZNC-1980-35c-0669
	Volume	35

117	Author	Dieter Strack, Peter Proksch, Paul-G Erhard Gülz	Requires cookie*
	Title	Reversed Phase High Performance Liquid Chromatography of Essential Oils
	Abstract	A reversed phase high performance liquid chromatographic procedure for the separation of essential oils is described. Applying water-acetonitrile elution systems on octyl and octadecyl-silane-bonded silica, complex mixtures o f sequiterpenes and oxygenated volatile constituents can be resolved, comparably to the quality o f gas chromatographic analysis. Photometrical detection at different wavelengths can be an important parameter in optimizing the separation of essential oil constituents. The application o f the described method is demonstrated with naturally occurring mixtures of terpenes from leaves of Cistus ladanifer.
	Reference	Z. Naturforsch. 35c, 675—681 (1980); received June 20 1980
	Published	1980
	Keywords	High Performance Liquid Chromatography (HPLC), Essential Oils, Terpenes, Terpenoids, Cistus, Cistaceae
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	Identifier	ZNC-1980-35c-0675
	Volume	35

118	Author	Ann Herm, Elisabeth Esterbauer, D. Schwarzl, Ieter Grill	Requires cookie*
	Title	Umbelliferone in Needles of Picea abies
	Abstract	A compound showing blue fluorescence under UV-light was isolated from spruce needles and identified as umbelliferone. Structure analysis was based on UV and mass spectroscopy as well as chromatographic comparison with authentic material. Quantitative evaluation by direct measure ment of the fluorescence of the umbelliferone spot separated by TLC revealed that the umbelliferone content shows great variation within different individual trees ranging from 0 .42-42 (ig/g fresh weight. Only minute amounts are present as free umbelliferone while the abundant part is present as /?-glucoside.
	Reference	Z. Naturforsch. 35c, 682 (1980); received May 12 1980
	Published	1980
	Keywords	Umbelliferone, Quantitative Estimation, Picea abies, Genetic Families
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	Identifier	ZNC-1980-35c-0682
	Volume	35

119	Author	Eckhard Wollenweber, VolkerH. Dietz, D. Etlef Schillo, G.Erhard Schilling	Requires cookie*
	Title	A Series of Novel Flavanones from Fern Exudates
	Abstract	The white farinose exudate on the fronds o f Cheilanthes argentea contains a series of flavanones as minor constituents. These were identified by spectroscopic methods as: 5,4'-diOH,6,7-diOMe flavanone and its 4'-OMe derivative, 5,4'-diOH,7,8-diOMe flavanone and its 4'-OMe derivative, 5,6-diOH,7,8,4'-triOMe flavanone, 5,4'-diOH, 6,7,8-triOMe flavanone and its 4'-OMe derivative. The farina o f Notholaena limitanea var. mexicana contains eriodictyol-7,4'-di-methyl ether and eriodictyol-7,3', 4'-trimethyl ether as minor components. Both compounds were also identified in the exudate of N. fendleri, together with naringenin-7-OMe and naringenin-4'-OMe. In N. lemmonii var. lemmonii trace amounts of 5-OH,7,3',4',5'-tetra-OMe flavanone were detected. Nine of these substances are novel natural flavanones.
	Reference	Z. Naturforsch. 35c, 685—690 (1980); received June 4 1980
	Published	1980
	Keywords	Cheilanthes argentea, Notholaena, Polypodiaceae, Farinose Exudate, Methylated Flavanones
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	Identifier	ZNC-1980-35c-0685
	Volume	35

120	Author	G. Forkm, W. Heller, H. Grisebach	Requires cookie*
	Title	Anthocyanin Biosynthesis in Flowers of Matthiola incana Flavanone 3-and Flavonoid 3'-Hydroxylases
	Abstract	Enzyme preparations from flowers o f defined genotypes o f Matthiola incana contain two dif ferent hydroxylases for hydroxylation of naringenin in the 3-and 3'-position, respectively. The 3-hydroxylase is a soluble enzyme and requires as cofactors 2-oxoglutarate, Fe2+ and ascorbate. Besides naringenin eriodictyol is a substrate for the 3-hydroxylase. The 3'-hydroxylase is localized in the microsomal fraction and requires NADPH as cofactor. Naringenin and dihydro-kaempferol but not 4-coumarate or 4-coumaroyl-CoA are substrates for this enzyme. 3'-Hy-droxylase activity is present only in genetic lines of M. incana with the wild-type allele b +.
	Reference	Z. Naturforsch. 35c, 691—6 (1980); received June 25 1980
	Published	1980
	Keywords	Anthocyanins, Flavonoids, Biosynthesis, Flavanon 3-Hydroxylase, Flavonoid 3'-Hydroxylase, Matthiola incana
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	Identifier	ZNC-1980-35c-0691
	Volume	35

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