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2000[X]
21Author    Concepción Pérez-Guerrero, Marí, Á. Lvarez De Sotomayor, Maria Dolores, H. Errera, ElisaM. ArhuendaRequires cookie*
 Title    Endothelium Modulates Contractile Response to Simvastatin in Rat Aorta  
  Reference    Z. Naturforsch. 55c, 121 (2000) 
  Published    2000 
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 TEI-XML for    default:Reihe_C/55/ZNC-2000-55c-0121.pdf 
 Identifier    ZNC-2000-55c-0121 
 Volume    55 
22Author    FriedhelmA. Hlers3, HenningB. Ubertb, Stefan Steuernagef, RolfW. Ierm Ann3Requires cookie*
 Title    The Nature of Oxygen in Sporopollenin from the Pollen of Typha angustifolia L  
  Reference    Z. Naturforsch. 55c, 129 (2000) 
  Published    2000 
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 TEI-XML for    default:Reihe_C/55/ZNC-2000-55c-0129.pdf 
 Identifier    ZNC-2000-55c-0129 
 Volume    55 
23Author    Kalina Iv, SorenR. Alipieva3, Henrik Jensenb, NedjalkaV. Franzykb, Handjieva3, LjubaN. EvstatievacRequires cookie*
 Title    Iridoid Glucosides from Phlomis tuberosa L. and Phlomis herba-ventis L  
 Abstract    A new iridoid glucoside, 5-desoxysesamoside, was isolated from Phlom is tuberosa L. (Lam iaceae) together with three known iridoid glucosides sesamoside, shanziside methyl ester and lamalbid. Lamiide was found in P. herba-ventis ssp. pungens in high concentrations. 
  Reference    Z. Naturforsch. 55c, 137 (2000); received September 21/Novem ber 4 1999 
  Published    2000 
  Keywords    Phlom is, Lamiaceae, Iridoid Glycosides 
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 TEI-XML for    default:Reihe_C/55/ZNC-2000-55c-0137.pdf 
 Identifier    ZNC-2000-55c-0137 
 Volume    55 
24Author    OrlandoM. Uñoz3, Carla Delporte, Nadine Backhouse, Silvia Erazo, Rosa Negrete, SergioM. Aldonadob, JoséL. López-Pérez, A. Rturo, San Feliciano0Requires cookie*
 Title    A New Cucurbitacin Glycoside from Kageneckia oblonga (Rosaceae)  
 Abstract    56(2) 2713888. E-mail: omunoz@abello.die.uehile.el A novel cucurbitacin glycoside has been isolated from aerial parts of Kageneckia oblonga R. et P. and shown to be 3ß-(ß-D-glucosyloxy)-16a,23a-epoxycucurbita-5,24-dien-ll-one. The structure was established by usual spectroscopic and two-dimensional (2D) NM R techniques. This compound has found to be nontoxic when tested in-vivo cell culture assays. In previous investigations we reported 23,24-dihydrocucurbitacin F and prunasine. This was the first re­ port on cucurbitacins from the genus Kageneckia (R osaceae). 
  Reference    Z. Naturforsch. 55c, 141 (2000); received October 13/November 19 1999 
  Published    2000 
  Keywords    Kageneckia oblonga, Rosaceae, Cucurbitacin 
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 TEI-XML for    default:Reihe_C/55/ZNC-2000-55c-0141.pdf 
 Identifier    ZNC-2000-55c-0141 
 Volume    55 
25Author    Christof Lenz, Cordula Amann, G. Em Ot Briskot, Kambiz Taraz, H. Erbert BudzikiewiczRequires cookie*
 Title    Succinopyoverdins -a New Variety of the Pyoverdin Chromophore  
 Abstract    + 4 9 -2 2 1 -4 7 0 -5 0 5 7 . E-mail: h.budzikiewicz@uni-koeln.de Pseudomoncis spp., Pyoverdin, Succinopyoverdin Pseudomonas spp. of the fluorescent group produce siderophores (so-called pyoverdins) consisting of a peptide chain attached to a pyrimidoquinoline ring system which is derived from a condensation product of L-Dab and D-Tyr. Commonly several related compounds are found to accompany the pyoverdins having the same peptide chain, but differing in the heterocyclic part. The structure elucidation of a new variety (succinopyoverdin) is de­ scribed here. 
  Reference    Z. Naturforsch. 55c, 146—152 (2000); received N ovem ber 12 1999 
  Published    2000 
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 TEI-XML for    default:Reihe_C/55/ZNC-2000-55c-0146.pdf 
 Identifier    ZNC-2000-55c-0146 
 Volume    55 
26Author    W. Erner Voßen, Regine Fuchs, Kambiz Taraz, H. Erbert BudzikiewiczRequires cookie*
 Title    Can the Peptide Chain of a Pyoverdin be Bound by an Ester Bond to the Chromophore? -The Old Problem of Pseudobactin 7SR1  
 Abstract    + 4 9 -2 2 1 -4 7 0 -5 0 5 7 . E-mail: h.budzikiewicz@uni-koeln.de Pseudom onas fluorescens, Pyoverdin, Siderophore The structure which had been proposed for the pyoverdin named pseudobactin 7SR1 (Yang and Leong, 1984) differed from those of all other pyoverdins investigated sofar: its peptide chain was supposedly linked to the chromophore not by an amide bond originating from its N-terminal amino acid, but rather by an ester bond involving one of the three Ser. It will be shown that the peptide chain of pseudobactin 7SR1 is actually bound to the chromophore amidically by its N-terminal Ser and that it comprises a cyc/odepsipeptidic substructure with an ester bond between the C-terminal Thr and the OH-group of the second Ser in the chain. 
  Reference    Z. Naturforsch. 55c, 153 (2000); received November 11 1999 
  Published    2000 
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 TEI-XML for    default:Reihe_C/55/ZNC-2000-55c-0153.pdf 
 Identifier    ZNC-2000-55c-0153 
 Volume    55 
27Author    Mitsuyoshi Sakasai3, Hiroki Fukui3, Hideyuki Yamane3, Naing Aung, Satoshi Kyaw3, Tahara3bRequires cookie*
 Title    A New Class of Biflavonoids: 2'-Hydroxy genistein Dimers from the Roots of White Lupin  
 Abstract    b C REST of Japan Science and Technology Corporation. Fax: + 8 1 -1 1 -7 0 6 -4 1 8 2 . E-mail: tahara@abs.agr.hokudai.ac.jp Two novel isoflavonoid dimers presumably originating from 2'-hydroxygenistein, 5,7,4'-trihydroxycoumaranochroman-4-one-(3-*5'")-5",7",2'",4'"-tetrahydroxyisoflav-one (1, lupinal-bisone A) and 5,7,4'-trihydroxycoumaranochro-man-4-one-(3—>-6")-5",7",2"',4"'-tetrahydroxy-isoflavone (2, lupinalbisone B) were isolated from the roots of Lupinus albus L., and their structures involving relative stereochemistry were elucidated by spectroscopic methods. U sing horse radish peroxidase and 2'-hydroxygenistein (3) as the substrate revealed the for­ mation o f these dimers together with 5,7,4'-trihydroxycoumaronochromone (4, lupinalbin A). Dimerization of 3 caused a remarkable increase of antifungal activity. 
  Reference    Z. Naturforsch. 55c, 165 (2000); received November 8/Decem ber 21 1999 
  Published    2000 
  Keywords    Lupinus albus, Biflavonoids, 2'-Hydroxygenistein 
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 TEI-XML for    default:Reihe_C/55/ZNC-2000-55c-0165.pdf 
 Identifier    ZNC-2000-55c-0165 
 Volume    55 
28Author    Denilson Soares De Siqueira3, A. Lberto Dos, Santos Pereira3, JoseAugusto Cabralb, Carlos Alberto, Cid Ferreirab, Francisco Radler De, AquinoN. EtoaRequires cookie*
 Title    Systematic Study of High Molecular Weight Compounds in Amazonian Plants by High Temperature Gas Chromatography-Mass Spectrometry  
 Abstract    High Temperature Gas Chromatography/MS, Simaruba amara, Bertholletia excelsa The fractions of hexane and dichloromethane extraction from marupä (Simaruba amara) and (Bertholletia excelsa) leaves were analyzed by HT-HRGC (high temperature high resolu­ tion gas chromatography) and HT-HRGC coupled to mass spectrometry (HT-HRGC-M S). Several compounds can be characterized including unusual high molecular weight com ­ pounds. 
  Reference    Z. Naturforsch. 55c, 175—1 (2000); received Novem ber 5/D ecem ber 6 1999 
  Published    2000 
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 TEI-XML for    default:Reihe_C/55/ZNC-2000-55c-0175.pdf 
 Identifier    ZNC-2000-55c-0175 
 Volume    55 
29Author    Jenny Fäldt3, Mikael Erikssonb, Irena Valterovác, Anna-Karin Borg-Karlson3, Czech Republic, FlemingovoNarnRequires cookie*
 Title    Comparison of Headspace Techniques for Sampling Volatile Natural Products in a Dynamic System c Institute o f Organic Chemistry and Biochemistry, Academy of Sciences of the  
 Abstract    Commonly used dynamic sorption techniques for collecting biologically active volatile compounds have been compared. Solid phase microextraction (SPM E) using two types of fibers (polydimethylsiloxane, PDMS, 100 j.im, and carbowax/divinylbenzene, CW /DVB, 65 fj.m) was compared to purge and trap methods (Porapak Q, Tenax TA and charcoal) and a technique based on absorption in methanol in a cooling bath. Sampling was done in a stream of purified air (20 ml/min) in a closed and temperature-regulated (27 °C) glass tube, passing over a capillary tube containing a hexane solution of tridecane, heptadecane, l-octen-3-ol, 1-hexadecanol, ethyl tetradecanoate, a-pinene, linalool, terpinen-4-ol, ds-verbenol, verbenone, ß-caryophyllene, Z^E-farnesol, and geranylgeraniol. With all of the methods, the sampling was performed for a period o f 30 min before extraction and analysis was done on a GC-FID system. In general, SPME gave a higher response for all compounds except for a-pinene, which was only extracted by the CW /DVB fiber. Purge and trap methods and methanol absorption gave the same response for all substances extracted. None of the methods ex­ tracted hexadecanol and geranylgeraniol under the conditions used. However, the SPME equipped with the PDM S coating extracted heptadecane, i^Zs-farnesol and ethyl tetradeca­ noate. Our results show that SPME, when selecting the fibers to fit the polarity and volatility of the compounds, is an outstanding extraction method compared to purge and trap and methanol absorption, especially for a qualitative analysis. The best conditions for storing fibers exposed to compounds of high volatility were at low temperatures (6 °C) in sealed vials, while the worst way was to leave the exposed fiber unprotected at room temperature (22 °C). The dynamic sampling system was effectively tested on a fruiting body of a polypore fungus (G anoderm a applanatum) emitting l-octen-3-ol, and again SPME showed to be the most sensitive technique. 
  Reference    Z. Naturforsch. 55c, 180—188 (2000); 16610 Prague 6 Czech Republic received November 25 1999/February 10 2000 
  Published    2000 
  Keywords    Volatile Compounds, Dynamic Headspace, Sampling Technique 
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 Identifier    ZNC-2000-55c-0180 
 Volume    55 
30Author    Yi Huang3, PingH. Eb, KlausP B Aderc, Alfons Radunzc, GeorgH. SchmidcRequires cookie*
 Title    Seeds of Trichosanthes kirilowii, an Energy-Rich Diet  
  Reference    Z. Naturforsch. 55c, 189 (2000) 
  Published    2000 
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 TEI-XML for    default:Reihe_C/55/ZNC-2000-55c-0189.pdf 
 Identifier    ZNC-2000-55c-0189 
 Volume    55 
31Author    Riikka Räisänen3, Heikki Björkb, PaavoH H Ynninen3, Paavo Hynninen@helsinki, FiRequires cookie*
 Title    Two-Dimensional TLC Separation and Mass Spectrometric Identification of Anthraquinones Isolated from the Fungus Dermocybe sanguinea  
 Abstract    Two-Dimensional TLC, Anthraquinone, D erm ocybe sanguinea A new two-dimensional TLC technique was developed to separate substituted anthraqui­ nones on silica plates using n-pentanol-pyridine-methanol (6:4:3, v/v/v) and toluene-ethyl acetate-ethanol-formic acid (10:8:1:2, v/v/v/v) as eluents. The good separation power of the new technique was demonstrated by applying it to the analysis of complex anthraquinone mixtures isolated from the Scandinavian D erm ocybe sanguinea. Emodin, physcion, en-docrocin, dermolutein, dermorubin, 5-chlorodermorubin, emodin-1-ß-D-glucopyranoside, dermocybin-1-ß-D-glucopyranoside and dermocybin, and five new, earlier in D. sanguinea unidentified compounds, 7-chloroemodin, 5,7-dichloroemodin, 5,7-dichloroendocrocin, 4-hy-droxyaustrocorticone and austrocorticone, were separated and identified on the basis of Rr values, UV/Vis spectra and mass spectra. 
  Reference    Z. Naturforsch. 55c, 195—202 (2000); received D ecem ber 20 1999 
  Published    2000 
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 TEI-XML for    default:Reihe_C/55/ZNC-2000-55c-0195.pdf 
 Identifier    ZNC-2000-55c-0195 
 Volume    55 
32Author    NorbertG. Rotjohann3, Wolfgang Kowallik3, Yi Huangb, Andrea Schulte In Den Bäum, W. Kowallik@, Biologie Uni-Bielefeld, DeRequires cookie*
 Title    Investigations into Enzymes of Nitrogen Metabolism of the Ectomycorrhizal Basidiomycete, Suillus bovinus Fax: 0 5 2 1 -1 0 6 -6 0 3 9 . E-mail  
  Reference    Z. Naturforsch. 55c, 203 (2000) 
  Published    2000 
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 TEI-XML for    default:Reihe_C/55/ZNC-2000-55c-0203.pdf 
 Identifier    ZNC-2000-55c-0203 
 Volume    55 
33Author    Walter De Carvalho3, Silvio Silvério Da Silva3, Michele Vitolob, Ismael Maciel De, M. Ancilha3Requires cookie*
 Title    Use of Immobilized Candida Cells on Xylitol Production from Sugarcane Bagasse  
 Abstract    Immobilization, Candida guillierm ondii, Xylitol, Sugarcane Bagasse In this study we used the yeast Candida guilliermondii FTI 20037 immobilized by entrap­ ment in Ca-alginate beads (2 .5 -3 mm diameter) for xylitol production from concentrated sugarcane bagasse hemicellulosic hydrolysate in a repeated batch system. The fermentation runs were carried out in 125-and 250-ml Erlenmeyer flasks placed in an orbital shaker at 30 °C and 200 rpm during 72 h, keeping constant the proportion between work volume and flask total volume. According to the results, cell viability was substantially high (98%) in all fermentative cycles. The values of parameters xylitol yield and volumetric productivity increased significantly with the reutilization of the immobilized biocatalysts. The highest val­ ues of xylitol final concentration (11.05 g/1), yield factor (0.47 gig) and volumetric producti­ vity (0.22 g/lh) were obtained in 250-ml Erlenmeyer flasks containing 80 ml of medium plus 20 mi of immobilized biocatalysts. The support used in this study (Ca-alginate) presented stability in the experimental conditions used. The results show that the use of immobilized cells is a promising approach for increasing the xylitol production rates. 
  Reference    Z. Naturforsch. 55c, 213 (2000); received September 10/December 6 1999 
  Published    2000 
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 Identifier    ZNC-2000-55c-0213 
 Volume    55 
34Author    Ru Angelie, E. Drada3, Victor Wrayb, Albrecht Bergc, G. Udo, Räfec, G. Sudarsonod, Peter Ernot Brauers3, Proksch3Requires cookie*
 Title    Novel Spiciferone Derivatives from the Fungus Drechslera hawaiiensis Isolated from the Marine Sponge Callyspongia aerizusa  
 Abstract    Sponge-A ssociated Fungi, Drechslera hawaiiensis, Spiciferone From the marine sponge Callyspongia aerizusa collected from the Sea of Bali, Indonesia, fungal isolates of Drechslera hawaiiensis were obtained. Culture filtrates of the fungus yielded four spiciferone derivatives which include spiciferone A (1) and B (2), and two other novel derivatives including spiciferol A (3) which is an alcohol congener of spiciferone A (1) and compound 4 which is an monocyclic spiciferone congener featuring a butoxyl side chain. The structures of the novel compounds were established on the basis o f NM R spectroscopic ('H , 13C, COSY) and mass spectrometric (EIMS) data. 
  Reference    Z. Naturforsch. 55c, 218—221 (2000); received November 5/D ecem ber 20 1999 
  Published    2000 
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 TEI-XML for    default:Reihe_C/55/ZNC-2000-55c-0218.pdf 
 Identifier    ZNC-2000-55c-0218 
 Volume    55 
35Author    Heike Neumann, Dietrich WernerRequires cookie*
 Title    Gene Expression of Medicago sativa Inoculated with Sinorhizobium meliloti as Modulated by the Xenobiotics Cadmium and Fluoranthene  
 Abstract    M edicago sativa (Alfalfa), Sinorhizobium m eliloti, X enobiotics A lfalfa plants (M edicago sativa cv. Europe) inoculated with Sinorhizobium m eliloti 2011 (formerly Rhizobium meliloti, de Lajudie et al., 1994) were cultivated for 14 days under standardized growth conditions in mineral medium with addition of the heavy metal cad­ mium or the polycyclic aromatic hydrocarbon fluoranthene. These xenobiotics significantly reduced the numbers of root nodules before any visible damage to the plant could be d e­ tected. EC10. EC50, and EC90 (effective concentrations reducing nodulation, shoot and root fresh weight by 10, 50, or 90% compared to the control without pollutant) were calculated. EC50 for cadmium ranged from 5.8 jam (nodulation) to more than 20 |j,m (root fresh weight). 
  Reference    Z. Naturforsch. 55c, 222 (2000); received October 14/November 22 1999 
  Published    2000 
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 Identifier    ZNC-2000-55c-0222 
 Volume    55 
36Author    Jürgen Schmidt3, Klaus Richter6, Brunhilde Voigt3, Günter Adam3Requires cookie*
 Title    Metabolic Transformation of the Brassinosteroid 24-£/?i-castasterone by the Cockroach Periplaneta americana  
 Abstract    A fter feeding of 24-ep/-castasterone to the cockroach Periplaneta americana an organ-specific epimerization of the brassinosteroid to 2,24-d/ep/-castasterone could be detected in fem ale insects. The m etabolite being observed only in the ovaries and not in the testes of the insect was identified by GC/M S in comparison with a synthesized authentic sample. Con­ trary, 24-ep/-brassinolide is not m etabolized in the sexual organs of Periplaneta americana. This is the first evidence of a m etabolic transformation of a brassinosteroid in insects. 
  Reference    Z. Naturforsch. 55c, 233 (2000); received November 2/November 29 1999 
  Published    2000 
  Keywords    Periplaneta americana, Brassinosteroids, M etabolic Transformation 
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 Identifier    ZNC-2000-55c-0233 
 Volume    55 
37Author    Bożenna Różycka-Roszak, Hanna PruchnikRequires cookie*
 Title    Effect of Counterions on the Influence of Dodecyltrimethylammonium Halides on Thermotropic Phase Behaviour of Phosphatidylcholine Bilayers  
 Abstract    Effects of dodecyltrimethylammonium chloride (DTAC), dodecyltrimethylammonium bro­ mide (D T A B) and dodecyltrimethylammonium iodide (D T A I) on thermotropic phase behav­ iour of phosphatidylcholine bilayers as well as on !H NM R spectra were studied. In order to enhance the effect of counterions on water structure two series of experiments were per­ formed. In the first one the surfactants were added to the water phase and in the other one directly to lipid phase (a mixed film was formed). The effects o f particular surfactants on the main phase-transition temperature were more pronounced when they were added to the water phase (1st method) instead of the lipid phase (2nd method). Furthermore, in the case of the first method the transitions were found asymmetrical while in the second method nearly symmetrical. It is suggested that surfactant-poor and surfactant-rich domains are formed when surfactants are added to the water phase. 
  Reference    Z. Naturforsch. 55c, 240 (2000); received Novem ber 5/D ecem ber 15 1999 
  Published    2000 
  Keywords    Counterions, Dodecyltrimethylammonium Halides, Phase Behaviour, Phosphatidylcholine Bilayers 
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 TEI-XML for    default:Reihe_C/55/ZNC-2000-55c-0240.pdf 
 Identifier    ZNC-2000-55c-0240 
 Volume    55 
38Author    VassiliaJ. Sinanoglou, Sofia Miniadis-MeimaroglouRequires cookie*
 Title    Phospholipids in Mediterranean Cephalopods  
 Abstract    Polar lipids of the cephalopods Eledone moschata, Sepia officinalis and Todarodes sagittatus mantle, represent 50.5%, 66.1% and 74.2% of wet tissue respectively. On the other hand the polar lipids of these three species of cephalopods constitute o f 80.8%, 94.8% and 93.7% of phospholipids, respectively. The main phospholipids identified were phosphatidylcholine (52.2, 51.3 and 58.4% of total phospholipids respectively in the above m entioned species), phosphatidylethanolamine (18.1, 19.7 and 23.9%), sphingomyelin (10.7, 15.2 and 6.7%), lyso-phosphatidylcholine (3.1, 3.8 and 1.8%) and the unusual lipid ceramide am inoethylphospho-nic acid (15.9, 10 and 9.2%). The 56.8% of phosphatidylcholine in E ledone m oschata, the 46% in Sepia officinalis and the 74.1% in Todarodes sagittatus refer to the structure of 1,2-diacyl-glycerocholine and the remaining percentage refer to the structure of l-oalkyl-2-acyl-glycerocholine or l-o-alk yl-l-enyl-2-acyl-glycerocholine. The 87.2% of phosphatidylethanolamine in Eledone m oschata, the 81% in Sepia officinalis and the 90.7% in Todarodes sagittatus refer to the structure o f 1,2-diacyl-glyceroethanolamine and the remaining percentage refer to the structure of l-o-alkyl-2-acyl-glyceroethanolam ine or l-o a lk y l-l-enyl-2-acyl-glyceroethanolamine. The major saturated fatty acids in phosphatidylcholine and phosphatidylethanolamine were C16:0 (30.3-67.5% and 23.2-54.5%) and C18:0 (3.6 -1 7 % and 1 5.4-28%), respectively, while the major unsaturated fatty acids in these lipids were C18:ln-9, n-7 (1.0-7.3% and 5.3-10.5%), C20:5n-3 (1.5-9.8% and 4 ,5-15.8%) and C22:6n-3 (12.5-42.0% and 7 .0 -11.3%), respectively. 
  Reference    Z. Naturforsch. 55c, 245 (2000); received September 29/Novem ber 2 1999 
  Published    2000 
  Keywords    Cephalopods, Octopus Lipids, Phospholipids 
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 Identifier    ZNC-2000-55c-0245 
 Volume    55 
39Author    Requires cookie*
 Title    Material and Methods  
  Reference    Z. Naturforsch. 55c, 256 (2000) 
  Published    2000 
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 TEI-XML for    default:Reihe_C/55/ZNC-2000-55c-0256.pdf 
 Identifier    ZNC-2000-55c-0256 
 Volume    55 
40Author    Jerka Dumića, Gordan Laue3, Mirko Hadžijab, Mirna Flögel3Requires cookie*
 Title    Transfer to in vitro Conditions Influences Expression and Intracellular Distribution of Galectin-3 in Murine Peritoneal Macrophages  
 Abstract    G alectin-3, Cellular Stress, Peritoneal Macrophages Galectin-3 is a ß-galactoside-binding lectin that has been implicated in numerous physio­ logical processes, including mRNA splicing, cell differentiation, tumor metastasis and the stress response. We have studied effects of transfer of resident murine peritoneal macro­ phages to in vitro conditions on galectin-3 in different cell compartments. Galectin-3 was purified by immunoprecipitation with rat monoclonal antibody M3/38, and analyzed by im-munoblotting using the same antibody. Transfer to in vitro conditions nearly doubled the total amount o f galectin-3 in cells, and caused significant alterations in its intracellular distribution, indicating that galectin-3 is involved in the adaptation o f peritoneal macrophages to in vi­ tro conditions. 
  Reference    Z. Naturforsch. 55c, 261 (2000); received November 25/D ecem ber 27 1999 
  Published    2000 
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 TEI-XML for    default:Reihe_C/55/ZNC-2000-55c-0261.pdf 
 Identifier    ZNC-2000-55c-0261 
 Volume    55 
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