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1983[X]
41Author    H. W. Eißhaar, P. BögerRequires cookie*
 Title    Sulfide Stimulation of Light-Induced Hydrogen Evolution by the Cyanobacterium Nostoc muscorum  
 Abstract    Light-induced hydrogen evolution by the blue-green alga N ostoc muscorum is stim ulated by sulfide with a rate of about 20 (imol H 2 per mg chlorophyll and h our stable for about one day at low light intensities (7 W /m 2). Oxygen evolution does not im pair sulfide-stim ulated hydrogen formation. Increased hydrogen evolution is catalyzed by nitrogenase, its activity is not influenced by sulfide. Apparently, sulfide inhibits the hydrogen-uptake pathw ay(s), thereby increasing net hydrogen evolution. — Furtherm ore, photosynthetic electron flow is inhibited by sulfide, essen­ tially at the oxidizing side o f photosystem II, as was dem onstrated by fluorescence-signal changes. This leads to decreased electron supply o f nitrogenase. Hydrogen production apparently is at optim um when both the effect o f sulfide on photo­ synthesis is moderate and when uptake-hydrogenase activity is abolished. 
  Reference    Z. Naturforsch. 38c, 237 (1983); received D ecem ber 8 1982 
  Published    1983 
  Keywords    Hydrogen Production, Photobiology, Sulfide, Blue-Greens 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0237.pdf 
 Identifier    ZNC-1983-38c-0237 
 Volume    38 
42Author    P. Braun, J. Bode, A. WildRequires cookie*
 Title    Ribulose-1,5-bisphosphate Carboxylase-Oxygenase: New Aspects Respective the pH-Dependance of the Carboxylation Reaction  
 Abstract    The investigation was directed towards the effects o f reaction conditions, substrates and pH on the carboxylation reaction of ribulose-1 ,5-bisphosphate carboxylase-oxygenase in the crude enzyme extracts from several plants. O ptim al substrate concentrations (H C O j and RubP) were determined. The highest carboxylase activity was attained with Tris/H Cl buffer. The pH activity profile was quite sharp with an optim um at pH 7.8. Purified and crystallized carboxylase yielded a broad optimum curve under the same reaction conditions. 
  Reference    Z. Naturforsch. 38c, 243—246 (1983); received D ecem ber 6 1982 
  Published    1983 
  Keywords    Ribulose-1, 5-bisphosphate Carboxylase-Oxygenase, C rude Enzyme Extracts, Reaction Conditions, pH Activity Profile 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0243.pdf 
 Identifier    ZNC-1983-38c-0243 
 Volume    38 
43Author    Marie-Jose DelrieuRequires cookie*
 Title    Evidence for Unequal M isses in Oxygen Flash Yield Sequence in Photosynthesis  
 Abstract    The numerical analysis of the oxygen flash yield Yn sequences, alone, does not allow to choose between two models: equal S state misses with non negligible double hits or unequal misses with nearly no double hits. Nevertheless, the com parison o f the sequences in different conditions shows that the equal miss model is unrealistic: in very different experimental conditions (non saturating flash, different batch o f Chlorella or chloroplasts), a parallel variation of the hom o­ geneous miss and double hit factors is observed. This correlation seems strange within the equal miss model: misses come from incomplete reaction (i.e. for exemple insufficient light) and double hits i.e. double advancem ent come, in principle, from excessive light or too long flash; for these reasons, opposite variation of misses and double hits as a function of light intensity are ex­ pected. W ithin the equal miss model the inverse is exactly observed: at low flash light intensity (11%) which increases the misses, 16% of double hits are needed, which is quite unrealistic. In contrast, the unequal miss model explains such result quite naturally by a m athem atical property: any theoretical sequence with only a unique S state miss and no double hit can be fitted with homogeneous misses and double hits, which increase in parallel as a function o f the damping. Evidence for unequal misses in oxygen flash yield sequence is provided by the heterogeneous properties of the light saturation curves (M. J. D elrieu, Biochim. Biophys. Acta 592, 4 7 8 -4 9 4 (1980)). At high flash intensity, all, excepted the transition S ' 2 -*■ S3 , are saturated; the transition £ 2 -* S3 is far from saturation and its very large saturating light intensity is actually not known. A comparative study, in the same chloroplast batch, o f the oxygen yield patterns with attenuated flashes and of the experimental saturation curves of S states shows that only photo­ chemical misses (due to non saturation) exist. At high intensity, there is only a unique miss for the transition S 2 S 3 i.e. the probability for this transition is low. A model involving a second acceptor could explain the slow increase of transition probability o f S ' 2 -* S3 at high flash intensity. 
  Reference    Z. Naturforsch. 38c, 247—258 (1983); received N ovem ber 16 1982 
  Published    1983 
  Keywords    Oxygen Evolution, Photosystem II, M atrix Analysis, N on Saturating Flash Sequence, Electron Acceptor 
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 Identifier    ZNC-1983-38c-0247 
 Volume    38 
44Author    H. P. Leiseifer, G. H. SchleserRequires cookie*
 Title    Calorimeter-Fermentor Combination for Investigations on Microbial Steady State Cultures  
 Abstract    Flow Microcalorimetry, E. coli K12, Aerobic Chem ostat C ulture The com bination of a 3-liter fermentor with a flow-through calorim eter is described. M inim um time-lags are less than 10 s corresponding to m axim um therm al peak disturbances of ~ 4 jiW. The set up shows an excellent long time stability. A erobic growth o f Escherichia coli K12 in batch cultures was used to test the equipm ent. Precision is ± 2.1% with regard to the total heat release of 4.94 kJ • I-1 and ± 2.5% with respect to the m axim um heat flow o f 609 mW • l-1. The accuracy is comparable with calorimeters using commercial flow mixing cells. 
  Reference    Z. Naturforsch. 38c, 259—267 (1983); received July 2/D ecem ber 8 1982 
  Published    1983 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0259.pdf 
 Identifier    ZNC-1983-38c-0259 
 Volume    38 
45Author    AdrianusC. Borstlap, PaulG. DoucetRequires cookie*
 Title    The 'Double' Michaelis-Menten Equation: Estimation of Parameters  
 Abstract    Sum of Two Michaelis-Menten Terms, Integrated Rate Equation, Param eter Estimation When the concentration dependence o f an enzymic reaction or a transport process can be described by the sum of two M ichaelis-M enten terms, reliable data that relate reaction rate and substrate concentration can be obtained even when as much as 70 per cent of substrate was consumed during the assay. Each data pair consists of the average reaction rate during an assay and the concentration where the instantaneous reaction rate was equal to the average rate. Although that concentration cannot be com puted exactly (as it depends on the four kinetic parameters), it may be com puted in a good approxim ation as if the reaction followed the simple Michaelis-Menten relationship. The relative error in the approxim ated concentration for 1 ^ K2/K \ ^ 105 and 10~ 2 ^ V2/V \ ^ 102 did not exceed 5 per cent up to 50 per cent of substrate consumption, and did not exceed 10 per cent up to 70 per cent o f substrate consumption. 
  Reference    Z. Naturforsch. 38c, 268—272 (1983); received Septem ber 9 1982/January 5 1983 
  Published    1983 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0268.pdf 
 Identifier    ZNC-1983-38c-0268 
 Volume    38 
46Author    B. Cifuentes, I. G. Arcía, C. VicenteRequires cookie*
 Title    L-Usnate-Urease Interactions: Binding Sites for Polymerization  
 Abstract    5/N ovem ber 22, 1982 Urease, L-Usnic Acid L-usnic acid inactivates urease through a process which im plicates the blockade o f — SH groups in parallel to the form ation o f inactive polymers. Both L-alanine and L-proline partially reverses the inactivation and effectively dim inishes the amount o f highly polym erized protein. The am ino acids also prevent the linkage o f L-usnic acid on the sites o f low affinity for the ligand, being then related to the sites o f polym erization. 
  Reference    Z. Naturforsch. 38c, 273—276 (1983); received April 
  Published    1983 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0273.pdf 
 Identifier    ZNC-1983-38c-0273 
 Volume    38 
47Author    M. E. John, W. KnöchelRequires cookie*
 Title    Enhancement of in-vitro Translation of Eukaryotic RNAs by Cyclic AM P  
 Abstract    Septem ber 22/N ovem ber 23, 1982 RNA, in-vitro Translation, Cyclic AMP, D ouble Stranded RNA, Protein Products Addition of cyclic AMP to normal rabbit reticulocyte lysate brings about substantial increase in protein synthesis program m ed by both nuclear and polysomal eukaryotic RNAs. The increase in synthesis is largely nonspecific and the in-vitro translation system is rendered viable for longer periods of time than in the absence o f cyclic AMP. This stim ulation of translation could be due to the inhibition of protein kinases that are initially activated by secondary structure o f RNAs. However, the ability of cyclic AMP to further stim ulate methyl mercury or heat denatured RNA indicates that additional mechanisms may be involved in the enhancem ent o f translation. 
  Reference    Z. Naturforsch. 38c, 277—281 (1983); received 
  Published    1983 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0277.pdf 
 Identifier    ZNC-1983-38c-0277 
 Volume    38 
48Author    W. Boll, M. M. AkinoseRequires cookie*
 Title    Characterisation of the Enzyme Intermediates of the Sarcoplasmic Transport ATPase by the Use of Inhibitors  
 Abstract    Sarcoplasmic Transport ATPase, Enzymes Intermediates 1. Based on a detailed reaction scheme of the phosphorylation process of the sarcoplasmic transport ATPase the inhibition mechanisms o f benzoctamine, DIO 9, AMP-PNP and of Ca2+-ions at relatively high concentrations (1 ~ 100 |iM) were determined. 2. The inhibition mechanisms were analyzed by measuring the y-phosphate exchange between ATP and ADP and evaluated by applying conventional and an extended Dixon plot procedures. 3. The kinetic patterns o f the inhibition were shown to be com patible with the assumed reaction scheme. 4. Each inhibitor combines with definite interm ediates: Benzoctamine with the interm ediate species m *"2E and £fg22E-ATP; AM P-PNP with ~ P; DIO 9 with E and MgE and Ca2+ at rela­ tively hign concentrations with E. 5. The central interm ediate blocked by benzoctamine can partially exist as Mg2 2E pDP-benzoct-amine which is detected as phosphoprotein after acid denaturation. 
  Reference    Z. Naturforsch. 38c, 282 (1983); received D ecem ber 3 1982 
  Published    1983 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0282.pdf 
 Identifier    ZNC-1983-38c-0282 
 Volume    38 
49Author    M. Maiti, Sujata Ghosh, Amala Chatterjee, S. N. ChatterjeeRequires cookie*
 Title    Thermal Stability of DNA Interacting with Furazolidone and Cu(II) Ions  
 Abstract    Thermal Stability of DNA, Furazolidone, Cu(II) Ions Furazolidone, on complexing with DNA, led to its therm al stabilization. The increase in transi­ tion tem perature of DNA (A Tm) increased linearly with % A — T content. Increasing concentration of Cu(II) ions progressively lowered the transition tem perature o f D NA , but Cu(II) ions were not equally effective in lowering the transition tem perature of furazolidone-D N A complex. W hen equim olar amounts o f Cu(II) ions and furazolidone were used, the stabilisation effects of furazoli­ done prevailed over the destabilisation effect o f Cu(II) ions. 
  Reference    Z. Naturforsch. 38c, 290 (1983); received October 18/N ovem ber 26 1982 
  Published    1983 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0290.pdf 
 Identifier    ZNC-1983-38c-0290 
 Volume    38 
50Author    G. F. Grossi, M.F M Acchiato, G. G. IalanellaRequires cookie*
 Title    Circular Permutation Analysis of Phage T4 DNA by Electron Microscopy  
 Abstract    T4 DNA, Perm utation, Electron Microscopy, D enaturation and Self-Reannealing Phage T4 is known to have a linear duplex chrom osom e that is circularly perm uted and ter­ minally repeated. We found, by denaturation and self-reannealing experiments, that circular perm utation in T4 native DNA is not random. T heir m ultim odal distribution of perm utation is compatible with the "headful packaging" model with the additional specifications that the encapsulation of D NA starts at several sites and these are not random distributed. 
  Reference    Z. Naturforsch. 38c, 294—296 (1983); received July 7 1982 
  Published    1983 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0294.pdf 
 Identifier    ZNC-1983-38c-0294 
 Volume    38 
51Author    A. RadunzRequires cookie*
 Title    On a Quantitative Determination of Antibodies to Lipids and Proteins  
 Abstract    The amount of precipitating antibodies in monospecific lipid-and protein antisera was deter­ mined by quantitative precipitation reactions according to the methods o f H eidelberger and Kendall. Antisera were obtained by im munization o f rabbits. Fragm ents of the thylakoid membrane from Antirrhinum chloroplasts were used as antigens for the binding of antibodies. These fragments had a diam eter of 100 A. They were composed of 51% proteins and 39% lipids. 
  Reference    Z. Naturforsch. 38c, 297—301 (1983); received Decem ber 28 1982 
  Published    1983 
  Keywords    Lipid Antisera, Protein Antisera, Chloroplast Antisera, Antibody Concentration, Thylakoid Membrane 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0297.pdf 
 Identifier    ZNC-1983-38c-0297 
 Volume    38 
52Author    Srabani Banerjee, S. N. ChatterjeeRequires cookie*
 Title    Effect of Sodium Cholate on the Phase Transition Temperature of Dipalmitoyl Phosphatidylcholine  
 Abstract    On treatm ent with sodium cholate, the phase transition tem perature (Tc) of m ultilam ellar liposomes derived from dipalm itoyl phosphatidylcholine (DPPC) progressively decreased with increasing cholate: lipid-P m olar ratio. A molar ratio of 4.5 caused a one degree depression in Tc. The unilamellar cholate vesicles o f DPPC exhibited the same Tc as that o f the m ultilam ellar ones. The formation of unilam ellar vesicles from the m ultilam ellar ones by cholate treatm ent was in­ vestigated by electron microscopy. 
  Reference    Z. Naturforsch. 38c, 302—306 (1983); received Septem ber 20/N ovem ber 15 1982 
  Published    1983 
  Keywords    Liposomes, DPPC, Sodium Cholate, Phase Transition, Electron Microscopy 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0302.pdf 
 Identifier    ZNC-1983-38c-0302 
 Volume    38 
53Author    Thomas Pigor, Rüdiger LawaczeckRequires cookie*
 Title    On the Interaction of Phospholipid Vesicles with Chaotropic Ions  
 Abstract    Lipid Vesicles, C haotropic Ions The addition o f a threshold am ount of C10;-ions induces a precipitation of dipalmitoylphos-phatidylcholine vesicles predom inantly in the crystalline state. O ther anions show a sim ilar but less pronounced behaviour. The effect follows the chaotropic order o f the regarded anions. The influence of alkaline cations is described but difficult to characterize. M easurements on the mechanism of this precipitation reveal that the vesicles stay intact and that vesicle-vesicle fusion can almost be excluded in the tim e-dom ain studied (seconds to minutes). The flocculation o f vesicles induced by the addition o f chaotropic ions precipitations. 
  Reference    Z. Naturforsch. 38c, 307—312 (1983); received O ctober 25 1982 
  Published    1983 
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 Identifier    ZNC-1983-38c-0307 
 Volume    38 
54Author    Rainer Merz, Friedhelm SchneiderRequires cookie*
 Title    Growth Characteristics of Anaerobically Treated Early and Late S-Period of Ehrlich Ascites Tumor Cells after Reaeration  
 Abstract    Proliferation Kinetics, Ehrlich Ascites Cells, Anaerobiosis Utilizing centrifugal elutriation, early and late S-phase cells were separated from 4, 8 and 12 h anaerobically cultured Ehrlich Ascites tum or cells strain Karzel. The cytokinetic properties o f these fractions after reaeration were studied by flow cytometry and the BrdU-H 33258-technique of flow cytometry. After a 4 h period of anaerobiosis, growth of early S-phase cells is not changed, 8 h deprivation of oxygen causes a delay of cell cycle progression, while the main fraction of 12 h anaerobically treated early S-populations did not divide after reaeration w ithin 24 h. In comparison to early S-phase cells the cell cycle progression of the main fraction of late S-period is accelerated after a 4 h exclusion of oxygen. A fraction o f 8 h anaerobically pretreated late S-cells continues to cycle, but a considerable num ber reinitiates D NA synthesis w ithout preceeding division. Cells with DNA content up to 8 c are detected by flow cytometry. 12 h anaerobically cultured late S-cells do not divide after reaeration, a large num ber o f these cells starts again to synthesize DNA. A considerable part o f tetraploid cells retain viability, divide and enter a new cell cycle, another part o f the cells disintegrates. 
  Reference    Z. Naturforsch. 38c, 313 (1983); received Novem ber 23 1982 
  Published    1983 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0313.pdf 
 Identifier    ZNC-1983-38c-0313 
 Volume    38 
55Author    Konrad DettnerRequires cookie*
 Title    Vergleichende Untersuchungen zur Wehrchemie und Drüsenmorphologie abdominaler Abwehrdrüsen von Kurzflüglern aus dem Subtribus Philonthina (Coleoptera, Staphylinidae) Comparative Investigations on Defensive Chem istry and G land Morphology of Abdominal Defensive G lands from Rove Beetles of the Subtribe Philonthina (Coleoptera, Staphylinidae)  
 Abstract    Within the rove beetle subtribe Philonthina (Staphylinidae) there are found paired abdom inal defensive glands which show a bipartition and are characterized by an unique, evaginable evapo­ ration tissue which is described for the first time. The gland chemistry is found to be character­ ized by at least 27 compounds. The m ain com ponent is represented by the alkaloid actinidine, m i­ nor components are iridodial, three possible m onoterpenoid iridodial precursors, an octadecenole and two series o f hydrocarbons and acetates which are partly branched and unsaturated. It could be shown, that the separated gland o f the evaporation tissue, whose evagination was studied, has no different synthetic abilities as com pared with the main gland system. Chemotaxonomic results according to Hennig's principle reveal an isolated position of the rove beetle tribe Q uediini within the subfamilies X antholininae and Staphylininae. The morphologically unique defensive gland of the X antholininae may be homologized with other abdom inal glands o f the Staphylinina and Philonthina, based on the comm on presence of iridoid defensive components. 
  Reference    Z. Naturforsch. 38c, 319 (1983); received July 27 1982 
  Published    1983 
  Keywords    Chemical Defence, Glands, Chemotaxonomy, Staphylinidae, Coleoptera 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0319.pdf 
 Identifier    ZNC-1983-38c-0319 
 Volume    38 
56Author    Klaus VogtRequires cookie*
 Title    Is the Fly Visual Pigment a Rhodopsin?  
 Abstract    Rhodopsin, Insect Visual Pigment, Retinal, Thin Layer C hrom atography, X anthophyll Thin layer chrom atography o f retina extracts from Calliphora yielded, instead of retinal (or ret-inaloximes after N H 2OH treatm ent) a considerably more polar aldehyde or its corresponding oximes. That these com pounds originate from the chrom ophoric group o f the visual pigm ent was shown by the occurrence o f different stereoisomers in the extracts depending on the colour with which the intact visual pigm ent complex was previously illum inated. The absorbance m axim um of the oxime found after blue illum ination is the same as that o f all-trans-retinal-oxim e (356 nm). This spectral accordance means that previous spectral evidence for retinal as the chrom ophoric group of fly visual pigm ent is not conclusive. Flies raised on a carotenoid deficient diet enriched with the hydroxy-xanthophyll lutein possess a high visual pigm ent content. This points to a hy-droxy-xanthophyll as a possible precursor of the chrom ophoric group. 
  Reference    Z. Naturforsch. 38c, 329 (1983); received January 20 1983 
  Published    1983 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0329.pdf 
 Identifier    ZNC-1983-38c-0329 
 Volume    38 
57Author    DavidJ. Robeson, JeffreyB. HarborneRequires cookie*
 Title    G rass Pea  
 Abstract    Leguminosae, Lathyrus sativus, Phytoalexin, 6 a-Hydroxy-pterocarpan, Pisatin Lathyrus sativus was investigated for the nature of its phytoalexin response with the result that leaflets, im ­ m ature pods, and seeds were induced to produce 6 a-hy-droxy-3-methoxy-8,9-methylenedioxypterocarpan (pisatin). Evidence is presented for the metabolism of pisatin, by the wound pathogen Botrytis cinerea, to a phenolic congener, 3,6a-hydroxy-8,9-methylenedioxypterocarpan. 
  Reference    Z. Naturforsch. 38c, 334—336 (1983); received N ovember 3 1982 
  Published    1983 
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 Identifier    ZNC-1983-38c-0334_n 
 Volume    38 
58Author    S. K. Bhattacharya, D. BhattacharyaRequires cookie*
 Title    Prostaglandin S ynthesis Inhibitors Reduce Cannabis and R estraint Stress Induced Increase in Rat Brain Serotonin C oncentrations  
 Abstract    Cannabis resin (Cl) produced a dose-related increase in rat brain serotonin concentrations, whereas restraint stress produced maximal rise of the neurotransm itter concentra­ tions at 1 h, followed by a tendency to norm alise by 4 h. The prostaglandin (PG) synthesis inhibitors, diclofenac and paracetamol, antagonized Cl and restraint stress in­ duced rise in serotonin concentrations. The findings lend credence to earlier reports that PG synthesis inhibitors antagonize serotonin-mediated neuropharmacological ac­ tions of Cl and restraint stress in rats. 
  Reference    Z. Naturforsch. 38c, 337 (1983); received September 15/N ovem ber 23 1982 
  Published    1983 
  Keywords    Prostaglandin Synthesis Inhibitors, Rat Brain Serotonin, Cannabis, Restraint Stress 
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 Identifier    ZNC-1983-38c-0337_n 
 Volume    38 
59Author    The Stereochemistry, F. Silybin, Hermann Lotter, H. Ildebert, W. AgnerRequires cookie*
 Title    Zur Stereochemie von Silybin  
 Abstract    The X-ray Structure of Silybin from Silybum marianum G aertn. was determined. The com ­ pound was found to be a diastereom eric m ixture (1 : 1) in the benzodioxane part of the molecule explaining several tentative aspects about the structure known from NM R-m easurem ents and HPLC-separations. Einführung 
  Reference    Z. Naturforsch. 38c, 339—341 (1983); received February 22 1983 
  Published    1983 
  Keywords    Silybin, Silybum marianum, X-Ray Structure, Absolute Configuration 
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 Identifier    ZNC-1983-38c-0339 
 Volume    38 
60Author    A. W. Schram, L.M V Jonsson, P. De VlamingRequires cookie*
 Title    Identification of Anthocyanins and Intermediates of Anthocyanin Biosynthesis from Petunia hybrida Using High Performance Liquid Chromatography  
 Abstract    A procedure to identify dihydroflavonol (glucosides), cinnam ic acids and anthocyanins in flowers of Petunia hybrida is presented. Nearly all known interm ediates and anthocyanins ocur-ring in Petunia are separated on one column using different elution systems. Hydrolysis of extracts before HPLC and thin layer chrom atography is in some cases necessary to m ake positive identification of flavonoids. Anthocyanins in flowers o f Petunia hybrida can occur, depending on genotype, as acylated anthocyanins. Two types o f acylated anthocyanins have been identified differing in polarity and thus in retention times on HPLC and TLC. The difference in acylation is genetically deter­ mined and gives rise to m inor colour differences. 
  Reference    Z. Naturforsch. 38c, 342—345 (1983); received February 21 1983 
  Published    1983 
  Keywords    High Performance Liquid Chrom atography, Anthocyanins, Flavonoids, Cinnam ic Acids, Petunia hybrida 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0342.pdf 
 Identifier    ZNC-1983-38c-0342 
 Volume    38 
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