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1980[X]
81Author    K. H. Grumbach, M. DrollingerRequires cookie*
 Title    The Effect of Phytochrome and Proteinsynthesis-Inhibitors on the Formation of Chlorophylls and Carotenoids in Radish Seedlings Treated with Photosystem II and Bleaching Herbicides  
 Abstract    Etioplasts of radish seedlings treated with photosystem II (DCM U, bentazon) and chlorotic herbicides (amitrole, SAN 6706) were tested on their ability to perform the phytochrome mediated chlorophyll and carotenoid biosynthesis. The cytoplasmic influence on the chloroplastic action o f herbicides was also investigated by inhibition of protein synthesis either in the chloro­ plast with chloramphenicol or in the cytoplasm with actidion. In all herbicide treated radish seedlings a phytochrome mediated chlorophyll and carotenoid biosynthesis was obtained as found in control plants. In plants treated with DCM U the bio­ synthesis o f carotenoids is enhanced compared to the control plants, while SAN 6706 significantly suppresses the carotenoid formation. It is concluded, that photosystem II and chlorotic herbicides do not interfere with the primary action o f phytochrome but rather do develope their effects on the terpenoid metabolism through phytochrome. Chloramphenicol applied at the time o f sowing very strongly suppresses the formation of chlorophylls and carotenoids in control plants. The sensitivity towards actidion is, however, very much lower. If chloramphenicol and actidion treated plants were also supplied with herbicides, the pigment pattern is completely different In SAN 6706-treated plants chloramphenicol acts synergistic, resulting in an even lower chlorophyll and carotenoid content than in plants supplied only with chloramphenicol. On the other hand SAN 6706 in combination with actidion enhances the formation of pigments, leading to a much higher chlorophyll and carotenoid content as in plants treated only with actidion. A stimulatory effect on the formation o f chlorophylls and carotenoids was also found for DCMU-treated plants in combination with actidion. The observation, that in plants treated with SAN 6706 together with actidion, which inhibits protein synthesis in the cytoplasm, the herbicidal response in the chloroplast is completely abolished, gives evidence that the photooxidative action of SAN 6706 in the chloroplast is developed by the cytoplasm. 
  Reference    Z. Naturforsch. 35c, 445 (1980); received February 8 1980 
  Published    1980 
  Keywords    Photosystem II Herbicides, Chlorotic Herbicides, Chlorophylls, Carotenoids, Phytochrome, Inhibitors 
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 Identifier    ZNC-1980-35c-0445 
 Volume    35 
82Author    Gerhard VierkeRequires cookie*
 Title    The Effect on Ammonium Chloride on the Kinetics of the Back Reaction of Photosystem II in Chlorella fusca and in Chloroplasts in the Presence of 3-(3,4-Dichlorophenyl)-l ,1 -dimethylurea  
 Abstract    Kinetics of the Back Reaction, Photosystem II, Ammonium Chloride The effect of N H 4C1 on the kinetics o f the back reaction of photosystem II as derived from luminescence measurements was investigated in dark adapted Chlorella in the presence o f 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCM U) at different temperatures. The kinetics o f the back reaction which, under these conditions, leads to the reduction o f the S2 state by the primary electron acceptor Q~ o f photosystem II was observed to be considerably slowed down in the presence of NH4C1. Analysis of the kinetic results in the light of the theory of the back reaction developed by Mar and Roy (J. Theor. Biol. 48, 257-281 (1974)) revealed two opposite effects of N H 4C1 to be present simultaneously: 1) The enthalpy o f activation of the back reaction was lowered (catalyzing effect o f N H 4C1) 2) The frequenca factor which indicates the number of collisions of the reacting molecules in the membrane per second is largely decreased (inhibitory effect of N H 4C1). This reduction o f the mobility o f the recombining species o f the back reaction is the predominant effect o f N H 4C1. It is suggested that this effect is due to a change o f the conforma­ tional state of the membrane induced by dissolution o f relative large amounts of N H ? within the lipid phase of the thylakoid membrane. This hypothesis is supported by the observation that the value of the exciton yield o f the back reaction changes upon addition of N H 4C1. 
  Reference    Z. Naturforsch. 35c, 451 (1980); received February 29 1980 
  Published    1980 
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 Identifier    ZNC-1980-35c-0451 
 Volume    35 
83Author    Wilhelm Menke, GeorgH. SchmidRequires cookie*
 Title    Fluorescence and Photochemical Properties of Plants with Defective Photosystem II  
 Abstract    The mykotrophic orchid Neottia nidus-avis does not evolve oxygen in the light but is able to perform photophosphorylation. The low temperature fluorescence emission spectrum lacks the 680 and 690 nm bands. Hence, the spectroscopic chlorophyll a forms which are attributed to photosystem II do not occur in plastids of this orchid. The low temperature excitation spectrum of photosystem I fluorescence exhibits a maximum at 666 nm. The position o f this maximum ap­ pears not to be influenced by energy transfer and corresponds to the absorption maximum of the chlorophyll form which emits the photosystem I fluorescence. Energy migration, however, occurs from carotenoids whose absorption spectrum is shifted to longer wavelengths and which cause the yellow-brown color of the Neottia plastids. Room temperature fluorescence emission shows after the onset of light no variable part. Despite the fact that plastids of the tobacco mutant N C 95 at most evolve only traces o f oxygen the low temperature emission spectrum shows the three bands which are usually observed with fully functioning chloroplasts. However, the two bands at 680 and 690 nm are distinctly lower than with the wild type. The variable portion o f room temperature fluorescence is barely detectable. In line with the very low capacity for oxygen evolution, rates of electron transport partial reactions in the region of photosystem II are extremely low. In agreement with this observation no 690 nm absorption change signal is detected. However, a normal P7 + 00 signal is seen. In the presence of electron donors like reduced phenazine methosulfate the decay time o f the P7 ^0 signal is faster than with the wild type. The yellow tobacco mutant Su/su var. aurea which exhibits at high light intensities higher rates of photosynthesis than the wild type shows at low temperature an emission spectrum with stronger photosystem II bands than the wild type. 
  Reference    Z. Naturforsch. 35c, 461 (1980); received March 14 1980 
  Published    1980 
  Keywords    Photosystem II, Low Temperature Fluorescence, Tobacco, Neottia 
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 Identifier    ZNC-1980-35c-0461 
 Volume    35 
84Author    Wemer Jahn, Heinz Faulstich, Axel Deboben, Theodor WielandRequires cookie*
 Title    Formation of Actin Clusters in Rat Liver Parenchymal Cells on Phalloidin Poisoning as Visualized by a Fluorescent Phallotoxin  
 Abstract    By staining of cryo-sections o f rat liver with a fluorescent phallotoxin, the distribution of filamentous actin in liver cells could be demonstrated by fluorescence microscopy. While in untreated livers filamentous actin forms an almost continous layer at the cell periphery, the poisoning by phalloidin leads to the formation of actin clusters, preferentially located near the cell membrane. 
  Reference    Z. Naturforsch. 35c, 467 (1980); received December 28 1979/February 22 1980 
  Published    1980 
  Keywords    Fluorescent Phallotoxin, Actin, Rat Liver 
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 Identifier    ZNC-1980-35c-0467 
 Volume    35 
85Author    Günter LazarRequires cookie*
 Title    Light-Induced Valence Change of a Manganese-Containing Chlorophyll Derivative  
 Abstract    K3(Mn-(III)-chlorin e6) acetate, a water-soluble chlorophyll derivative with manganese as central atom was prepared. The influence o f reducing agents, light, and bicarbonate on the oxida­ tion states of manganese in the chlorin complex was investigated. In alkaline solutions the central Mn3+ was reducible to Mn2+. The illumination of the partially reduced complex first accelerated the reduction (light reduction: Mn3+ -> Mn2+), which was then followed by a strong reoxidation (light oxidation: Mn2+ -* Mn3+) continuing after switching off the light. This redox effect o f light was obtained only in presence o f hydroxylamine as reducing agent. Illumination time, intensity, and frequency had an influence on this effect. In the light/dark change the process was reversible. In regard to the requirement of manganese for the photosynthetic oxygen evolution the observed valence change o f manganese in the chlorin complex was compared to the supposed reaction mechanism on the donor side o f photosystem II. 
  Reference    Z. Naturforsch. 35c, 470—476 (1980); received December 17 1979/March 19 1980 
  Published    1980 
  Keywords    Mn-(III)-chlorin, Photosynthesis, Photoreduction, Photooxidation, Hydroxylamine 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0470.pdf 
 Identifier    ZNC-1980-35c-0470 
 Volume    35 
86Author    T. Fujim, F. Yoshii, I. Kaetsu, Y. Inoue, K. ShibataRequires cookie*
 Title    Effect of Irradiation and Immobilization on Spinach Chloroplast Activities  
 Abstract    The effect of y-ray irradiation and immobilization by means of radiation polymerization on PS II activity (0 2 evolution) o f isolated chloroplasts from spinach was investigated. Reduction o f 0 2 evolution activity by irradiation was small at lower temperatures below — 24 °C, but the activity decreased slightly by freezing at extremely low temperature below —78 °C. So the optimum low temperature range for the treatment was observed. The immobilized chloroplast in a hydrophilic polymer matrix showed the stable duration o f 0 2 evolution activity more than 700 h at 4 °C . Thermo-stability o f chloroplast was also improved greatly by immobilization. The active center o f PS II in immobilized chloroplasts was retained even after 60 min standing at 50 °C. 
  Reference    Z. Naturforsch. 35c, 477—481 (1980); received February 12 1980 
  Published    1980 
  Keywords    Radiation Effect, Chloroplast, 0 2 Evolution, Immobilization, Radiation Polymerization, Low Temperatures 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0477.pdf 
 Identifier    ZNC-1980-35c-0477 
 Volume    35 
87Author    Dieter Vogel, G. Uy, D. De Marcillac, LeonH. Irth, Kazuyuki AkasakaRequires cookie*
 Title    The Mechanism of Aggregation of the Coat Protein of Tobacco Mosaic Virus. A Comparative Study on Vulgare and Mutant Proteins  
 Abstract    TMV vulgare, A 14, Ni 725; Two-and Three-Layer Aggregates, Structural and Mechanistic D if­ ferences, Inter-Subunit Interactions, Non-Specific Aggregations The aggregation behaviour o f tobacco mosaic virus (TMV) protein vulgare was compared to that of two mutants, A 14 and Ni725, with amino acid exchanges localized in the coat protein at posi­ tions 107 (Thr -* • Met, in N i725) and 129 (lie -> Thr, in both mutants). This behaviour, as meas­ ured by sedimentation, hydrogen ion titration, light-scattering, and near-UV absorption difference and circular dichroism (CD) spectroscopy, differs characteristically both in the range of the A-protein (pH 8) and near neutrality, whereas nuclear magnetic resonance (NMR) and far-UV CD point at only subtle, or no structural differences between the three strains. Near pH 8, the A-proteins o f both mutants sediment nearly exclusively as 8 S aggregates, under conditions where vulgare protein forms a 4 S /8 S mixture (two-layer and three-layer aggregates, Vogel etal. in conditions where vulgare 4S aggregates dominate, both mutants sediment as a 4 S /8 S mixture. The average molecular weights of the 8S proteins corre­ spond to 12 (vulgare) to 15 (mutants) subunits. -Near neutrality both mutants titrate and polyme­ rize more cooperatively than vulgare protein; additionally, the pK(app.) o f Ni 725 is shifted up­ wards, due to the higher a-helix forming potential o f Met against Thr (pos. 107). Both mutants form large aggregates (> 200 S) o f obviously helical conformation, by the uptake of one proton per subunit, whereas 20 S-disks constituting, under the same conditions, the stable entities in vul­ gare protein, are made only in minor amounts. These large mutant aggregates are remarkably more stable than the vulgare "overshoot" aggregates which transiently, too, may approach s-values and turbidities similar to the mutant aggregates; conformational changes, observed prior or in parallel to the formation of vulgare overshoot and disk aggregates, are significantly retarded in the large mutant aggregates. — Raising the ionic strength seems the only way to form mutant disks and stacks of disks (2 0 -3 0 S) comparable to vulgare, pointing to the different pathways o f disk formation, either at neutral pH or high ionic strength. — Evidence is given that the 8S aggregates of both mutant and vulgare proteins may behave similar in aggregation, the differences mainly being inserted by the 4S (two-layer) aggregates present in vulgare protein, which near neutrality seem responsible for the direct formation o f (two-layer) disks. -The non-conservative exchange in po­ sition 129, altering the environment o f Trp residues (52+17?), should weaken the "extended salt-bridge system" ("pairing") observed between the two layers o f the disk (Bloomer et al., Nature, 1978). A competition is suggested between the strength o f this pairing, and the binding of a third layer, regulating the mode o f aggregation to two-layer, to three-layer, and to higher aggregates; this is corroborated by comparison with published results on temperature-sensitive (ts I) mutants and chemically modified proteins. — To explain the effects of residue 129 on the titration o f the protein we suggest a mechanical analogy, made up o f a balance between the charge and state of the "carboxyle cage" (Stubbs et al., Nature, 1977), as regulatory site, and the strength o f the 
  Reference    Z. Naturforsch. 35c, 482 (1980); received November 14 1979 
  Published    1980 
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 Identifier    ZNC-1980-35c-0482 
 Volume    35 
88Author    H. WawraRequires cookie*
 Title    Die Mureinstruktur. I. Ermittlung der Mureinschichtdicke an festen Präparaten von Spirillum serpens Zellwänden mit Röntgenkleinwinkelmethoden Estimation o f the M ureinlayerthickness of Spirillum serpens Using Small-Angle X-Ray Methods  
 Abstract    The mureinlayerthickness o f Spirillum serpens was estimated by means o f small-angle X-ray scattering methods using solid preparates o f broken cell walls. The interpretation o f the scattering curves was possible by application o f G. Porod's scattering theory of packings of lamellae. 
  Reference    Z. Naturforsch. 35c, 495 (1980); eingegangen am 14. Dezember 1979/5. März 1980 
  Published    1980 
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 Identifier    ZNC-1980-35c-0495 
 Volume    35 
89Author    Anders Johnsson, Wolfgang Engelmann, Burkhard Pflug, W. Aldem, Ar KlemkeRequires cookie*
 Title    Influence of Lithium Ions on Human Circadian Rhythms  
 Abstract    Lithium carbonate lengthens the circadian period in humans under temporal isolation (arctic summer four groups. L i+ ions are known to change the period length of several circadian rhythm s in plants and animals [1], In all cases reported so far the effect is a lengthening o f the free running period. An experiment has been performed on a single person in an isolation unit to test whether L i+ acts on hum an circadian rhythms and the results provided have not been conclusive [2]. We have conducted an experiment to determine the influence o f L i+ on the hum an circadian system under arctic sum m er conditions. The continuous light and abscence o f other 24 h tim e cues allows free run o f hum an circadian rhythms [3]. In this brief report, we present some salient results of the effects of Li+ on the tem perature-, sleep-wakefulness-, and activity rhythm in some humans. More details of this investigation will be published elsewhere. 
  Reference    Z. Naturforsch. 35c, 503—507 (1980); received January 14 1980 
  Published    1980 
  Keywords    Dedicated to Prof C H Hertz, Lund University, Sweden, on His 60th Birthday Circadian Rhythms, Lithium, Body Temperature, Activity, Svalbard-Spitsbergen 
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 Identifier    ZNC-1980-35c-0503 
 Volume    35 
90Author    N-LRequires cookie*
 Title    ssu =  
 Abstract    1/2 where N = Num ber of corresponding common amino acids in the two proteins at a specific alignment, L' = Length of overlapping portions of the two proteins, B = Total num ber of m inim um base changes required to change protein i to protein j, and 
  Reference    Z. Naturforsch. 35c, 508 (1980) 
  Published    1980 
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 Identifier    ZNC-1980-35c-0508 
 Volume    35 
91Author    O., L. Gmelinii Flavonoids, G. Erard, J. N. Iem AnnRequires cookie*
 Title    P henolics from Larix N eedles. XV. H igh-Perform ance Liquid Chromatography  
 Abstract    From needles of Larix gmelinii sixteen flavonoids were isolated and identified. The main flavonoids had been analyzed before, eight minor ones were identified as quercetin-3-glucoside, isorhamnetin-3-glucoside and -3-ara-binoside, a somewhat lipophilic kaempferol-3-glucoside derivative, apigenin-7-glucoside, laricitrin-3-rutinoside and laricitrin-and syringetin-3-(/?-coumarylglucoside). 
  Reference    Z. Naturforsch. 35c, 514—515 (1980); received January 24 1980 
  Published    1980 
  Keywords    Larix gmelinii, Pinaceae, Flavonoids, High-Performance Liquid Chromatography 
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 Identifier    ZNC-1980-35c-0514_n 
 Volume    35 
92Author    Y. Plancke, J.P H Énichart, J. L. BernierRequires cookie*
 Title    M echanism Proposal for a Fluorescent Am anitin Derivative Form ation  
 Abstract    A quantitative fluorimetric detection o f a-amanitin treat­ ed by H 3PO" is proposed. Spectra for both amanitin and tryptophan suggest an extended aromatic structure clearly confirmed by JH NMR spectrography. 
  Reference    Z. Naturforsch. 35c, 516—518 (1980); received October 22 1979/January 21 1980 
  Published    1980 
  Keywords    a-Amanitin, Tryptophan, Fluorimetric Detection, *H NMR 
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 Identifier    ZNC-1980-35c-0516_n 
 Volume    35 
93Author    Cornelius LützRequires cookie*
 Title    The Determ ination o f Prolam ellar Bodies and Saponins in E tioplasts and Leaves o f A vena sativa L  
 Abstract    Prolamellar body tubules in etioplasts are composed mainly of two saponins. The determination o f these sapo-nins in leaves, etioplasts or subfractions of etioplasts is re­ commended to evaluate the concentration of these tubules in a given fraction. Using a densitometer after TLC-separa-tion of the saponins measurements are carried out in a short time with high accurancy. Also the absolute concen­ tration of these saponins can be determined. Problems in the quantification o f the saponins in leaf samples can be overcame with filters of different wavelengths. 
  Reference    Z. Naturforsch. 35c, 519—521 (1980); received January 21 1980 
  Published    1980 
  Keywords    Etioplasts, Prolamellar Bodies, Saponins, Chromatography 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0519_n.pdf 
 Identifier    ZNC-1980-35c-0519_n 
 Volume    35 
94Author    NobuoO. Kabe, Friedrich CramRequires cookie*
 Title    On the Reactivity o f PyridoxaI-5'-phosphate with Y east tR N A Phe and tR N A Tyr  
 Abstract    Yeast tRNAPhe, Yeast tRNATyr, Pyridoxal-5'-phosphate Yeast tRNAPhe and tRNATyr were reacted with the fluo­ rescent reagent pyridoxal-5'-phosphate and the modified tRNAs were analysed with respect to the number and posi­ tion of modified nucleosides and with respect to amino-acylation. a) Following the intrinsic fluorescence o f pyridoxal-5'-phosphate, the treatment of tRNATyr with increasing amounts of pyridoxal-5'-phosphate revealed about 50 mol o f reagent or a even higher number bound per one mol of tRNATyr. After borohydride reduction (in order to stabilize the linkage) of this modified tRNATyr and purifi­ cation with reverse phase chromatography a modified tRNATyr was obtained carrying about 2 mol of the reagent. b) Both tRNATyr and tRNAPhe treated with pyridoxal-5'-phosphate and reduced exhibited almost unchanged aminoacylation as compared to the unmodified tRNAs. c) Pyridoxal-5'-phosphate treated and reduced tR NA Phe and tRNATyr were digested with ribonuclease Tj and the resulting oligonucleotides were separated. However, no flu­ orescent oligonucleotide and no difference to an oligo­ nucleotide pattern obtained from unmodified tRNA were observed. Thus, pyridoxal-5'-phosphate might have been bound to the highly purified yeast tRNAPhe and tRNA1*1 ' samples either via an unstable linkage or not covalently. This result is controversial with respect to the specific reaction of pyridoxal-5'-phosphate with unfractionated tRNAs from colon carcinoma and tRNAs from E. coli as reported in the literature. 
  Reference    Z. Naturforsch. 35c, 522—525 (1980); received February 11/March 17 1980 
  Published    1980 
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 Identifier    ZNC-1980-35c-0522_n 
 Volume    35 
95Author    A. Rthur HollRequires cookie*
 Title    des Zw ergw elses Ictalurus nebulosus mit Procionfarbstoffen Selective Staining by Procion Dyes o f Olfactory Sensory Neurons in the Catfish Ictalurus nebulosus  
 Abstract    Procion Brilliant Yellow M 4R AN and Procion Yellow M 4R applied to the catfish olfactory mucosa stain intra-vitally and enter the olfactory receptors by their dendrites. The dye invades the receptor pericaryon and subsequently migrates within the axon in distal direction. Fluorescence microscopy of aldehyd fixed and paraffin sectioned prepa­ rations reveals clear selective staining o f the sensory neu­ rons in yellow colour. 
  Reference    Z. Naturforsch. 35c, 526—528 (1980); eingegangen am 11. Dezember 1979/31. Januar 1980 
  Published    1980 
  Keywords    Selective Fluorescence, Procion, Olfactory Receptors, Fishes 
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 Identifier    ZNC-1980-35c-0526_n 
 Volume    35 
96Author    Peter Proksch, Paul-G Erhard Gülz, H. Erbert BudzikiewiczRequires cookie*
 Title    Further Oxygenated Compounds in the Essential Oil of Cistus ladanifer L. (Cistaceae)  
 Abstract    Benzyl benzoate, cis-ocimenone and a new acetophenone derivative, 2-hydroxy-6-methyl aceto-phone, could be isolated by chromatographic methods from the essential oil o f Cistus ladanifer. Structural elucidation by NMR and MS are described. In addition pinocarvone, campholene alde­ hyde and tagetone were identified by their mass spectra. 
  Reference    Z. Naturforsch. 35c, 529—532 (1980); received April 21 1980 
  Published    1980 
  Keywords    Essential Oils, Cistaceae, Cistus ladanifer, Oxygenated Compounds, NMR-and MS-Data 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0529.pdf 
 Identifier    ZNC-1980-35c-0529 
 Volume    35 
97Author    D. Ieter, S. Track3, N. Aam, A. Kaviab, HansR. EznikaRequires cookie*
 Title    High Performance Liquid Chromatographic Identification of Anthocyanins  
 Abstract    A high performance liquid chromatographic procedure for a rapid identification o f antho­ cyanins is described. Besides their characteristic retention times, anthocyanins can easily be identified by an HPLC examination o f the kinetics of appearance and disappearance o f their partial hydrolysis products. This method is also applicable to 3-deoxyanthocyanins which is demonstrated with an extract from the fern Blechnum brasiliense. The described method allows optimal resolution of complex anthocyanin mixtures in the fol­ lowing sequence o f elution: 3-sophoroside-5-glucoside; 3-rutinoside-5-glucoside; 3,5-diglucoside; 3-sophoroside; 3-galactoside; 3-glucoside; 3-rutinoside; 5-glucoside; aglycone, and finally the forms acylated with /?-coumaric acid. Substitution in the B-ring o f the aglycone structure is the key factor for the range o f the retention time. Thus cyanidin 3,5-diglucoside elutes earlier (—38 sec) than pelargonidin 3-sophoroside-5-glucoside. 
  Reference    Z. Naturforsch. 35c, 533—538 (1980); received March 20 1980 
  Published    1980 
  Keywords    Anthocyanins, 3-Deoxyanthocyanins, Partial Hydrolysis, High Performance Liquid Chromato­ graphy 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0533.pdf 
 Identifier    ZNC-1980-35c-0533 
 Volume    35 
98Author    M. Artin Bopp, W. Erner LüdickeRequires cookie*
 Title    Synthesis of Sinapine during Seed Development of Sinapis alba  
 Abstract    Sinapis alba, Sinapine, Phenylalanine, Seed D evelopm ent, Substance A ccum ulation Sinapine (sinapoyl-choline) is accumulated in the cotyledones o f Sinapis alba during the main growth phase o f the developing embryos. It is degraded during germination o f older embryos w hich have not reached full maturation and o f mature seeds, but rem ains stable in young embryos. The main product o f degradation is sinapoyl-glucose. [14C]phenylalanine is not only in­ corporated into sinapine during the accum ulation phase in situ but just so into isolated embryos which do not accumulate the substance further. Synthesis takes place in the cotyledones. Their synthesizing capacity (incorporation o f [14C]phenylalanine) is correlated with the growth rate o f the cotyledones. 
  Reference    Z. Naturforsch. 35c, 539 (1980); received March 26 1980 
  Published    1980 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0539.pdf 
 Identifier    ZNC-1980-35c-0539 
 Volume    35 
99Author    Ingrid Gentzen, Hans-G Löffler, Friedh SchneiderRequires cookie*
 Title    Aminoacylase from Aspergillus oryzae. Comparison with the Pig Kidney Enzyme  
 Abstract    Am inoacylase (EC 3.5.1.14) from Aspergillus oryzae was purified from a com m ercially available crude material by heat treatment, precipitation by polyethyleneim ine and amm onium sulfate, gel chromatography and preparative disc-gel-electrophoresis. The purified product was hom ogenous as judged by polyacrylamide gel electrophoresis. SDS-gel electrophoresis, polyacrylam ide-gel-gradient electrophoresis, gel chromatography and amino acid analysis demonstrated the enzyme to be com posed o f two subunits with M r o f 36 600. The kinetic properties o f the enzyme were studied with chloroacetyl derivatives o f alanine, phenylalanine, methionine, leucine, norleucine and tryptophan. The pH optim um o f the acylase activity with chloroacetyl-alanine as substrate is at pH 8.5. Acyl derivatives o f hydrophobic am ino acids are preferred substrates. The enzyme has no dipeptidase activity. Am inoacylase is not inhibited by SH -blocking agents and no SH-groups could be detected with Ellman's reagent in the native and denatured enzyme. The enzyme activity is insensitive to phenylmethylsulfonyl fluoride and N-a-/?-tosyl-L-lysine chlorom ethyl ketone. The microbial acylase is a zinc m etallo enzyme. M etal chelating agents are strong inhibitors; it is further inhibited by Cd2+, Mn2+, N i2+, C u2+ and activated by C o2+. The properties o f pig kidney and Aspergillus acylase are compared. 
  Reference    Z. Naturforsch. 35c, 544 (1980); received February 21 1980 
  Published    1980 
  Keywords    Aspergillus Am inoacylase, Purification, Properties 
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 Identifier    ZNC-1980-35c-0544 
 Volume    35 
100Author    K.-H Knobloch, J. BerlinRequires cookie*
 Title    Influence of Medium Composition on the Formation of Secondary Compounds in Cell Suspension Cultures of Catharanthus roseus (L.) G. Don  
 Abstract    Cell suspension cultures o f Catharanthus roseus have been subjected to various m edia condi­ tions in order to stim ulate the formation o f indole alkaloids. H igh ajmalicine contents (up to 0.5 m g/g cell fresh weight) were achieved by transferring 2-week-old cell suspensions to a 10-fold volum e o f a 8% sucrose solution. The alkaloid accum ulation started two days after the transfer and reached a plateau after ten days. Furthermore an enhanced level o f phenolic com pounds was found, whereas growth o f the culture was low. The accumulation o f both, alkaloids and poly­ phenols was stimulated by high concentrations o f sucrose and low concentrations o f nitrogen con­ taining salts and phosphate. W hen these m inerals were added to the sucrose solution in con­ centrations com m only used for cell culture m edia, the accum ulation o f alkaloids and phenolic compounds was largely suppressed. 
  Reference    Z. Naturforsch. 35c, 551 (1980); received March 31 1980 
  Published    1980 
  Keywords    Catharanthus roseus, Cell Suspension Cultures, A lkaloids, Polyphenols, M edia Effects 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0551.pdf 
 Identifier    ZNC-1980-35c-0551 
 Volume    35 
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