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1980[X]
101Author    KlausJ N Eurohr, HenryH. MantschRequires cookie*
 Title    Complex Formation of Carnosine with Purine Nucleotides in Aqueous Solution  
 Abstract    This ^ -N M R study provides experimental evidence for an intermolecular interaction between the dipeptide carnosine (yS-alanyl-L-histidine) and the purine nucleoside 5'-m onophosphates 5'-AM P, 5'-IMP and 5'-GM P. From the observed upfield shifts o f the purine nucleotide and im idazole proton resonances it is concluded that the interaction is o f the stacking type and that it involves the purine base o f the nucleotide and the histidine m oiety o f carnosine. Apparent microscopic equilibrium constants and com plex shifts are obtained with a m icroscopic m odel, which considers the formation o f both 1:1 and 1 :2 complexes. The stacking pattern for com plex formation between the histidine m oiety o f carnosine and the adenine m oiety o f 5'-AM P is con­ structed by fitting the experimental 5'-AM P com plex for histidine. 
  Reference    Z. Naturforsch. 35c, 557 (1980); received D ecem ber 10 1979 
  Published    1980 
  Keywords    Carnosine, Purine N ucleotides, Proton N M R, Base Stacking, Equilibrium Constants 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0557.pdf 
 Identifier    ZNC-1980-35c-0557 
 Volume    35 
102Author    W. G. Pohl, R. K. Reikenbohm, K. SeuwenRequires cookie*
 Title    The Specificity of Ionophore A 23187 in Cation Transport across Lipid Membranes Studies with Lecithin Vesicles  
 Abstract    Lecithin vesicles containing different cations were prepared by sonication and characterized. When the concentration o f the divalent cations in the buffer was increased from 1 m M to about 100 m M the cation-concentration inside o f the vesicles did not increase proportional to the outside concentration, but showed a saturation behaviour. The efflux o f various cations m ediated by ionophore A 23187 was m easured and relative transport-rates were determined. The following sequence was obtained: Zn2+ > Mn2+ > Ca2+ > M g2+ > Sr2+ > Ba2+ % L i+ > N a +. The efflux o f Ca2+ increased proportional to the square o f the A 2 3 187-concentration. To lecithin-vesicles containing ethylenediam ine tetraacetate (ED TA) inside different cations were added on the outside. In the presence o f A 23187 divalent cations are transported into the vesicles and bound there by EDTA. During the influx o f M e2+-ions into vesicles a H +-efflux was observed. The resulting pH-decrease was measured. The rate o f the pH -change depended on the M e2+-ion used. The sequence was: Cd2+ > Zn2+ > Ca2+ > M n2+ > M g2+ > Sr2+, Ba2+. 
  Reference    Z. Naturforsch. 35c, 562 (1980); received February 11/M arch 6 1980 
  Published    1980 
  Keywords    Ionophore A 23187, Lecithin Vesicles, Cation Specificity, Proton Antiport 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0562.pdf 
 Identifier    ZNC-1980-35c-0562 
 Volume    35 
103Author    Helm Ut Sapper, Wolfgang Gohl, Wolfgang LohmannRequires cookie*
 Title    31P-und ^-NMR-Untersuchungen über die Wechselwirkung von ATP mit Mg2+, Ca2+ und Monoaminen 31P-and 'H-NM R-Investigations about the Interaction of ATP with Mg2+, C a2+, and Monoamines  
 Abstract    The interaction between aromatic amines and ATP is based upon an electrostatic attraction o f the protonated am ino groups to the negatively charged phosphate groups o f ATP com bined with a stacking association between the ring systems. The com plexes formed are relatively weak (as­ sociation constants 4 -4 8 M_1), shortlived (life tim e smaller than 0.02 s), and m ainly 1:1 in stoichiometry preferring the terminal phosphate o f ATP as the site o f association. The divalent metal ions M g2+ and Ca2+ bond stronger than the am ines (association constants 1500 and 100 M_1, resp.) prefer the /?-phosphate group changing thereby the geom etry o f the ATP phosphate chain. Thus, they reduce the am ine-ATP association in ternary mixtures. 
  Reference    Z. Naturforsch. 35c, 569—577 (1980); eingegangen am 27. März 1980 
  Published    1980 
  Keywords    ATP, Phosphate Interaction, Stacking Association, Phosphorus and Proton M agnetic Resonance 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0569.pdf 
 Identifier    ZNC-1980-35c-0569 
 Volume    35 
104Author    Yoriko Taketo, Akira TaketoRequires cookie*
 Title    Effects of Polynucleotides on Production and Activity of Streptolysin S  
 Abstract    Activity o f various natural and synthetic polynucleotides as a carrier for streptolysin S was tested in a resting cell system. As the carrier, intact m olecules o f M S2 R N A , E. coli tR N A or rat liver R N A were almost inactive, whereas R N ase I core o f these R N A s, especially the core frac­ tions eluted from DEA E-cellulose colum n at higher NaC l concentrations, effectively induced pro­ duction o f the extracellular hem olysin. The carrier activity o f yeast R N A was significantly en­ hanced by sim ultaneous addition o f R N ase I, to the streptococcal suspension. Preincubation o f yeast R N A with growing streptococci or a protein fraction from the bacterial culture supernatant increased its carrier activity. Evidences were obtained suggesting involvem ent o f streptococcal nuclease in the enhancement o f the carrier effect. Production o f the streptococcal hem olysin was markedly prom oted by polyguanylic acid but neither by polyadenylic acid, polycytidylic acid nor by polyuridylic acid. Like trypan blue, polyguanylic acid exerted potent inhibitory effect on hem olytic activity o f streptolysin S complex. Similar but less marked effect was observed with certain R N ase I core fractions o f rat liver R N A. Som e data concerning effect o f polynucleotides on stability o f the hem olysin were also presented. 
  Reference    Z. Naturforsch. 35c, 578 (1980); received March 10 1980 
  Published    1980 
  Keywords    Streptolysin S, R N A, Polyguanylic Acid, Streptococcus pyogenes 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0578.pdf 
 Identifier    ZNC-1980-35c-0578 
 Volume    35 
105Author    Jürgen Pauluhn, Annem Arie, N. Aujok, H. Erbert, W. Zim, Erm AnnRequires cookie*
 Title    Über die quantitative fluorometrische Bestimmung von DNA mit Ethidiumbromid in der Zelle und die Konstanz der Quantenausbeute des Ethidium-DNA-Komplexes im biologischen Milieu On the Q uantitative Fluorom etric D eterm ination of Cellular DNA by Ethidium Bromide and the Constancy o f the Quantum Yield of the Ethidium -D N A -Com plex in the Biological Environment  
 Abstract    The fluorescent staining o f fixed A 9 cells (m ouse fibroblasts) with ethidium brom ide E has been investigated qualitatively and quantitatively. E is bound to D N A , R N A and protein. After enzymatic R N A digestion and staining at pH = 4.0 the dyestuff is bound to D N A specifically. The formed E -D N A -com plex is stoichiom etric if D N A will be saturated by high dye concentra­ tion, CF = 1.0 x 10~2 M. The stoichiom etric factor n = 0.21 was determined from Scatchard bind­ ing isotherms and by spectrophotometric titration. The binding o f E to D N A and R N A has been investigated by binding isotherms. E is bound to D N A by intercalation and by ionic interaction. It is bound to R N A by ionic interaction only. The ionically bound dyestuff can be replaced by salts like LiCl from D N A and RNA. Only inter­ calated E remains. The com petitive salt effect can be used to avoid the enzym atic R N A digestion in the specific staining o f D N A . W ithin the cell the E-D N A -com plex fluoresces strongly. U sing a microspectrophotom eter we determined the fluorescence intensity J and the extinction E in cell sections o f q = 1 |a2 area. The fluorescence was excited in the m inimum o f the absorbance o f the E -D N A -com plex at = 365 nm. The extinction E 2 was measured in the short wavelength band at X2 = 260 nm. Under these conditions we received a linear relation J = J (E ?) up to high extinctions E 2. The quantum yield Q o f the E -D N A -com plex is nearly constant and independent o f the biological environment. Q is also unaltered by LiCl. W ithin the lim its o f error intercalated and ionic bound dye have the same quantum yield. Under the conditions o f stoichiom etric staining the am ount o f E -D N A -complex and therefore D N A in the cell can be determined by J. The extinction coefficient e2 = 45200 M-1 cm -1 o f the E -D N A -com plex at 260 nm was calcu­ lated from the concentration dependence o f the absorbance spectra using the law o f mass action. By e2 the am ount o f D N A in the cell is accessible from J. According to our measurements with A 9 cells the superficial density o f D N A has the order o f m agnitude £>n = m ^ /q = 101 nmol cm -2, the amount raN o f D N A per section q = 1 jj.2, mN = 10_l fmol, and the D N A concentration in the cell nucleus CN = 10-1 M. The ray pass and diagram o f electronics o f the used m icrospectrophotom eter are described. 
  Reference    Z. Naturforsch. 35c, 585—5 (1980); eingegangen am 14. Januar/21. April 1980 
  Published    1980 
  Keywords    D N A -determ ination, Ethidium Bromide, M ouse Fibroblasts, Specific Fluorescent Staining, Quantum Y ield, M icrospectrophotometer 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0585.pdf 
 Identifier    ZNC-1980-35c-0585 
 Volume    35 
106Author    W. Ilhelm Uecker, D.Iethard Kubsch, D. Ieter Lutz, Hans KrögerRequires cookie*
 Title    Einbau von |32P|Orthophosphat in DNA der Mäuse-Milz unter Antigen-Einfluß Incorporation of [32P]O rthophosphate into DN A o f the Mouse Spleen under Influence o f Antigen  
 Abstract    Antigens cause an increase o f the D NA synthesis in the spleen o f mice as shown by the in­ corporation of [32P]orthophosphate and [3H]thym idine. In the present paper, the incorporation of [32P]orthophosphate into the single deoxymononucleotides has been studied under influence of the antigen bovine serum albumin. For this purpose, the labelled D NA was decomposed to the deoxymononucleotides and their specific activities determ ined. Using [32P] orthophosphate, it was found that the activity o f the D NA increased continuously during an observation period of 8 h. While after one hour the relative specific activity o f dTM P was highest and that o f dAMP lowest, the relative specific activities o f the deoxymononucleotides had become equal after 8 h. Under the influence of bovine serum album in, the incorporation o f [32P]orthophosphate increased in the main band DNA as well as in the satellite DNA. The antigen had no effect on the distribution of the specific activities o f the deoxymononucleotides. 
  Reference    Z. Naturforsch. 35c, 599—604 (1980); eingegangen am 24. F ebruar/25. April 1980 
  Published    1980 
  Keywords    Orthophosphate, Mouse Spleen, DNA, Antigen, Desoxymononucleotides 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0599.pdf 
 Identifier    ZNC-1980-35c-0599 
 Volume    35 
107Author    LaurenG. Otsuki, D. Ieter, W. GruenwedelRequires cookie*
 Title    Methylmercury-Chromosome Interactions. I. Thermal Denaturation of Calf Thymus Chromatin in Presence of CH3HgOH  
  Reference    Z. Naturforsch. 35c, 605 (1980) 
  Published    1980 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0605.pdf 
 Identifier    ZNC-1980-35c-0605 
 Volume    35 
108Author    ThomasL J Boehm, Dusan DrahovskyRequires cookie*
 Title    Die Beziehung der enzymatischen Methylierung invers repetitiver DNA Sequenzen zur Transkription in Maus P815 Mastocytoma Zellen The Relation o f Enzymatic M ethylation of Inverted DNA Repeats to Transcription in Mouse P815 M astocytoma Cells  
 Abstract    The isolation of transcribed D NA sequences of P815 cells and the partial characterization with respect to their sequence composition and relative rates o f enzymatic D NA m ethylation are reported in this paper. Transcribed regions were purified by affinity chrom atography using im ­ mobilized heterogenous nuclear RNA of P815 cells. About 10% of total genome was found in this fraction. Reassociation analyses showed differences in sequence composition o f transcribed versus non-transcribed D N A fractions. The relative proportion of inverted repeats was doubled in the transcribed fraction whereas ordinary highly repetitive sequences comprising mainly o f satellite D NA were found alm ost exclusively in the non-transcribed regions o f the P815 genome. A bout 70% of transcribed portions corresponds to unique and interm ediary D NA sequences. A fter labelling of cells with L-[Methyl-3H]methionine and [14C]deoxycytidine relative rates o f enzymatic DNA m ethylation were computed for different kinetic components o f transcribed and non-transcribed portions of P815 genome. No difference was found except in inverted repeats. In transcribed D NA the relative rate o f enzymatic DNA methylation was only about 40% o f that of the non-transcribed ones. We have quantitated this hypomethylation and found that there is, in average, about one 5-methylcytosine residue in 100 nucleotides o f transcribed inverted repeats, com pared to about 2.5 5-methylcytosines in non-transcribed fractions. In view of these data we propose that the enzymatic methylation o f inverted DNA repeats negatively controls the tran ­ scriptional process in a given genomic region. 
  Reference    Z. Naturforsch. 35c, 611—6 (1980); eingegangen am 30. Januar 1980 
  Published    1980 
  Keywords    Enzymatic Methylation, Inverted D NA Repeats, Transcriptional Control 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0611.pdf 
 Identifier    ZNC-1980-35c-0611 
 Volume    35 
109Author    H. SchnablRequires cookie*
 Title    Anion Metabolism as Correlated with the Volume Changes of Guard Cell Protoplasts  
 Abstract    Isolated and purified guard cell protoplasts o f starch-containing (Vicia faba) and starch-lacking (Allium cepa) stom ata were used to study the m etabolism o f m alate and C l-anions as correlated with the K +-induced swelling o f protoplasts. The correlation between swelling and malate synthesis in starch-containing and the inhibition of this correlation in starch-lacking guard cell protoplasts were demonstrated. A model system was proposed involving the role o f m alate and its place of synthesis in these two contrasting stomatal types. 
  Reference    Z. Naturforsch. 35c, 621 (1980); received March 27 1980 
  Published    1980 
  Keywords    Allium cepa, G uard Cell Protoplasts, Malate, Vicia faba 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0621.pdf 
 Identifier    ZNC-1980-35c-0621 
 Volume    35 
110Author    Aloysius Wild, Barbara UrschelRequires cookie*
 Title    Chlorophyll-Protein Complexes of Chlorella fusca  
 Abstract    Chlorophyll-protein complexes from thylakoids of the normal type and two mutants o f Chlorella fusca were separated using sodium dodecyl sulfate acrylamide gel electrophoresis (PAGE). The properties o f the chlorophyll-protein complexes o f the three strains of Chlorella were compared. Standard curves were set up for the characterization of the chlorophyll-proteins. In every electrophoretic separation of chlorophyll-protein complexes, a certain am ount o f pigm ent is separated from the protein. We tried to keep that am ount as low as possible by mild solubiliza­ tion and by working in low tem perature. U nder these conditions, we obtained several new chlorophyll-proteins in addition to the P-700-chlorophyll a-protein (CP I) and the light-harvesting chlorophyll a/b-protein (CP II). Thus, a small band (CP I a) was located between the top of the gel and the CP I after elec­ trophoresis. A lthough it shows spectral qualities sim ilar to CP I, it possesses a much lower chloro­ phyll a/chlorophyll b ratio. It may be an aggregate of photosystem I and light-harvesting chloro­ phyll. We found and analyzed three other chlorophyll-proteins with electrophoretic m obilities inter­ mediate between that o f the P-700-chlorophyll a-protein and that of the light-harvesting chloro­ phyll a/b-protein complex. Two o f these chlorophyll-proteins, the LHCP1 and the L H C P2, have a low chlorophyll a/chlorophyll b ratio and spectral properties similar to that o f the light-harvesting chlorophyll a/b-protein (LH CP3). They obviously represent dimers or oligomers o f the latter com ­ plex. A third, new complex (CPa) m igrated between LHCP3 and its dimer. W ith the chlorophyll b deficient m utant G 36 of Chlorella fusca, this complex could be obtained in high purity and great enrichment (15% of total chlorophyll). It could be proved that this complex only contains chloro­ phyll a. Its red absorption m axim um is at 671 nm. Some indirect evidences suggest that it may be a good candidate for the PS II reaction center complex. 
  Reference    Z. Naturforsch. 35c, 627—6 (1980); received March 19 1980 
  Published    1980 
  Keywords    Chlorella, C hlorophyll-Protein Complexes, Polyacrylam ide-Gel Electrophoresis, Standard Curves for Characterization o f Chlorophyll Proteins 
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 Identifier    ZNC-1980-35c-0627 
 Volume    35 
111Author    G.Erhard VierkeRequires cookie*
 Title    Kinetics of the Back Reaction of Photosystem II as Derived from Delayed Light Emission in the Presence of DCMU and the Conformational State of the Thylakoid Membrane  
 Abstract    of the Thylakoid Membrane It is shown that the kinetics of the back reaction o f photosystem II in the seconds time range as derived from the luminescence decay curve in the presence of DCM U is controlled by the internal pH of the thylakoids. M odifications o f the conformational state of the photosynthetic m em brane while leaving the internal pH unchanged, however, reaction. 
  Reference    Z. Naturforsch. 35c, 638 (1980); received January 28/M ay 5 1980 
  Published    1980 
  Keywords    Photosynthesis, Photosystem II, Delayed Light Emission, Conform ational State 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0638.pdf 
 Identifier    ZNC-1980-35c-0638 
 Volume    35 
112Author    K.H G Rum Bach, B. FomRequires cookie*
 Title    Chloroplast Autonomy in Acetyl-Coenzyme-A-Formation and Terpenoid Biosynthesis  
 Abstract    Isolated intact spinach chloroplasts were supplied with 14C-labeled C 0 2, phosphoglycerate, phos-phoenolpyruvate, acetate and mevalonate and the incorporation o f radioactivity into /?-carotene and plastoquinone-9 assayed. All applied substrates were capable of crossing the chloroplast envelope. Except phosphoenolpyruvate all radioactive precursors were incorporated into ß-carotene and also into plastoquinone-9. It is concluded that spinach chloroplasts are autonom in acetyl-coenzyme-A-formation and terpenoid biosynthesis. There is a direct carbon flow from intermediates of the Calvin-cycle via acetyl-coenzyme-A and mevalonic acid existing in the chlo­ roplast leading to the carotenoids and isoprenoid side-chains o f chlorophylls and quinones. 
  Reference    Z. Naturforsch. 35c, 645—648 (1980); received March 25 1980 
  Published    1980 
  Keywords    Biosynthesis, /?-Carotene, Chloroplast, Plastoquinone-9, Terpenoids 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0645.pdf 
 Identifier    ZNC-1980-35c-0645 
 Volume    35 
113Author    GeoffreyA. Codd, G. Eorg, H. SchmidRequires cookie*
 Title    Effects of S-^^-DichlorophenyO-N-N'-Dimethylurea on Oxygen Evolution and Fluorescence by Whole Filaments and Isolated Thylakoids of the Cyanobacterium Anabaena cylindrica  
 Abstract    The site of inhibition of DCM U against photosystem II in the cyanobacterium (Blue-green alga) Anabaena cylindrica was examined in electron transport and fluorescence studies. Isolated thylakoids catalyzed silicomolybdate photoreduction using H zO as electron donor; the steady-state reaction was completely inhibited by DCM U. This reaction is insensitive to DCM U in chloro­ plasts, since silicomolybdate accepts electrons from the prim ary photosystem II acceptor, and thus before the site of action o f D CM U in higher plants. DCM U did not increase the steady-state level of fluorescence by inact A. cylindrica, nor affect the monophasic fluorescence induction, with 
  Reference    Z. Naturforsch. 35c, 649—655 (1980); received April 18 1980 
  Published    1980 
  Keywords    Oxygen-Evolving Side, Cyanobacteria, D CM U-Sensitivity, Fluorescence 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0649.pdf 
 Identifier    ZNC-1980-35c-0649 
 Volume    35 
114Author    D. Meier, H. K. Lichtenthaler, G. BurkardRequires cookie*
 Title    Change of Chloroplast Ultrastructure in Radish Seedlings under the Influence of the Photosystem II-Herbicide Bentazon  
 Abstract    The influence of the photosystem II-herbicide bentazon on the ultrastructure o f chloroplasts o f radish seedlings (Raphanus sativus L.) was investigated with special emphasis on thylakoid developm ent and grana formation. Bentazon application (10-3 m) induces the formation of broader and higher grana stacks (grana width: 0 .5 -0 .6 n m ; greatest frequency: 3 -8 thylakoids per granum) than in the control plants (grana width: 0.3 |im; greatest frequency: 2 -4 thylakoids per granum). Furtherm ore, the am ount of chloroplast lamellae is enhanced, as are the stacking degree of thylakoids and the grana area. The chloroplasts of bentazon-treated plants appear to be shorter and thicker than in the controls and show all signs of a shade-type adaptation of the photosynthetic apparatus. 
  Reference    Z. Naturforsch. 35c, 656 (1980); received March 17 1980 
  Published    1980 
  Keywords    Bentazon, Chloroplast-Ultrastructure, G rana Form ation, Photosystem II-Herbicide, Shade-type Chloroplasts 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0656.pdf 
 Identifier    ZNC-1980-35c-0656 
 Volume    35 
115Author    PedroE H Aeser, G. Ottfried, W. ErnerRequires cookie*
 Title    Verminderter Sauerstoffverbrauch und reduzierte Atemfrequenz verursacht durch "Schweres Wasser" (D zO) bei Fischen Reduced Oxygen Consumption and Reduced O perculare Movements in Fishes by Heavy W ater (D 20 )  
 Abstract    Operculare M ovements in Fishes, Oxygen Consumption, Heavy Water (D 20) Fishes (Scardinius erythrophthalmus) have a almost 40% reduced oxygen consum ption and a 18% reduced operculare movements in normal water containing 20% "heavy water" (D 20) . 
  Reference    Z. Naturforsch. 35c, 665 (1980); eingegangen am 24. Februar/10. April 1980 
  Published    1980 
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 Identifier    ZNC-1980-35c-0665 
 Volume    35 
116Author    Helga Sittertz-BhatkarRequires cookie*
 Title    Oral Cleansing in Spiders is Gland Mediated!  
 Abstract    Histochemical and micromorphological investigations of the gnathocoxal gland in spiders brought out its uniquely adaptive cleansing function. The prey particulates, caught in the oral mi­ crostructures, are bound with mucopolysaccharides or glyco-muco-proteins from the glandular se­ cretions in Araneus and Theridion species into a bolus, and are expelled following each food up­ take. 
  Reference    Z. Naturforsch. 35c, 669—673 (1980); received October 20 1979 
  Published    1980 
  Keywords    Oral Cleansing, Spiders, Gnathocoxal Gland, Histochemistry, Micromorphological Investigations 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0669.pdf 
 Identifier    ZNC-1980-35c-0669 
 Volume    35 
117Author    Dieter Strack, Peter Proksch, Paul-G Erhard GülzRequires cookie*
 Title    Reversed Phase High Performance Liquid Chromatography of Essential Oils  
 Abstract    A reversed phase high performance liquid chromatographic procedure for the separation of essential oils is described. Applying water-acetonitrile elution systems on octyl and octadecyl-silane-bonded silica, complex mixtures o f sequiterpenes and oxygenated volatile constituents can be resolved, comparably to the quality o f gas chromatographic analysis. Photometrical detection at different wavelengths can be an important parameter in optimizing the separation of essential oil constituents. The application o f the described method is demonstrated with naturally occurring mixtures of terpenes from leaves of Cistus ladanifer. 
  Reference    Z. Naturforsch. 35c, 675—681 (1980); received June 20 1980 
  Published    1980 
  Keywords    High Performance Liquid Chromatography (HPLC), Essential Oils, Terpenes, Terpenoids, Cistus, Cistaceae 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0675.pdf 
 Identifier    ZNC-1980-35c-0675 
 Volume    35 
118Author    Ann Herm, Elisabeth Esterbauer, D. Schwarzl, Ieter GrillRequires cookie*
 Title    Umbelliferone in Needles of Picea abies  
 Abstract    A compound showing blue fluorescence under UV-light was isolated from spruce needles and identified as umbelliferone. Structure analysis was based on UV and mass spectroscopy as well as chromatographic comparison with authentic material. Quantitative evaluation by direct measure­ ment of the fluorescence of the umbelliferone spot separated by TLC revealed that the umbelliferone content shows great variation within different individual trees ranging from 0 .42-42 (ig/g fresh weight. Only minute amounts are present as free umbelliferone while the abundant part is present as /?-glucoside. 
  Reference    Z. Naturforsch. 35c, 682 (1980); received May 12 1980 
  Published    1980 
  Keywords    Umbelliferone, Quantitative Estimation, Picea abies, Genetic Families 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0682.pdf 
 Identifier    ZNC-1980-35c-0682 
 Volume    35 
119Author    Eckhard Wollenweber, VolkerH. Dietz, D. Etlef Schillo, G.Erhard SchillingRequires cookie*
 Title    A Series of Novel Flavanones from Fern Exudates  
 Abstract    The white farinose exudate on the fronds o f Cheilanthes argentea contains a series of flavanones as minor constituents. These were identified by spectroscopic methods as: 5,4'-diOH,6,7-diOMe flavanone and its 4'-OMe derivative, 5,4'-diOH,7,8-diOMe flavanone and its 4'-OMe derivative, 5,6-diOH,7,8,4'-triOMe flavanone, 5,4'-diOH, 6,7,8-triOMe flavanone and its 4'-OMe derivative. The farina o f Notholaena limitanea var. mexicana contains eriodictyol-7,4'-di-methyl ether and eriodictyol-7,3', 4'-trimethyl ether as minor components. Both compounds were also identified in the exudate of N. fendleri, together with naringenin-7-OMe and naringenin-4'-OMe. In N. lemmonii var. lemmonii trace amounts of 5-OH,7,3',4',5'-tetra-OMe flavanone were detected. Nine of these substances are novel natural flavanones. 
  Reference    Z. Naturforsch. 35c, 685—690 (1980); received June 4 1980 
  Published    1980 
  Keywords    Cheilanthes argentea, Notholaena, Polypodiaceae, Farinose Exudate, Methylated Flavanones 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0685.pdf 
 Identifier    ZNC-1980-35c-0685 
 Volume    35 
120Author    G. Forkm, W. Heller, H. GrisebachRequires cookie*
 Title    Anthocyanin Biosynthesis in Flowers of Matthiola incana Flavanone 3-and Flavonoid 3'-Hydroxylases  
 Abstract    Enzyme preparations from flowers o f defined genotypes o f Matthiola incana contain two dif­ ferent hydroxylases for hydroxylation of naringenin in the 3-and 3'-position, respectively. The 3-hydroxylase is a soluble enzyme and requires as cofactors 2-oxoglutarate, Fe2+ and ascorbate. Besides naringenin eriodictyol is a substrate for the 3-hydroxylase. The 3'-hydroxylase is localized in the microsomal fraction and requires NADPH as cofactor. Naringenin and dihydro-kaempferol but not 4-coumarate or 4-coumaroyl-CoA are substrates for this enzyme. 3'-Hy-droxylase activity is present only in genetic lines of M. incana with the wild-type allele b +. 
  Reference    Z. Naturforsch. 35c, 691—6 (1980); received June 25 1980 
  Published    1980 
  Keywords    Anthocyanins, Flavonoids, Biosynthesis, Flavanon 3-Hydroxylase, Flavonoid 3'-Hydroxylase, Matthiola incana 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0691.pdf 
 Identifier    ZNC-1980-35c-0691 
 Volume    35 
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