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1978[X]
61Author    WimJ. Baas, CarlG. FigdorRequires cookie*
 Title    Isolation of a New seco-nor-Triterpenol from Hoya australis Leaf Wax  
 Abstract    From the alcohol fraction of the epicuticular leaf wax of Hoya australis R. Br. ex Traill, a new triterpenol was isolated which, according to mass spectral and NMR data, is assumed to be A-seco-A-nor-Zl 12-oleanenol. 
  Reference    Z. Naturforsch. 33c, 337 (1978); received December 19 1977/March 20 1978 
  Published    1978 
  Keywords    Hoya australis, Asclepiadaceae, Leaf Cuticular Wax, seco-Triterpenoid 
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 TEI-XML for    default:Reihe_C/33/ZNC-1978-33c-0337.pdf 
 Identifier    ZNC-1978-33c-0337 
 Volume    33 
62Author    Bernd Renner, Ernst GerstnerRequires cookie*
 Title    Dünnschichtchromatographische Isolierung von Flechtenstoffen und deren Identifizierung Identification of Lichen Substances by Isolation from Thin Layer Chromatogramms  
 Abstract    The use of Eluchrom (Camag, Berlin) for quantitative isolation of liehen substances separated by thin layer chromatography is described. For this method only small quantities of a lichen thallus are needed (less than 0.5 g) to yield sufficient amounts of pure compounds submittable to chemical and spectroscopical identification. In the case of Nephroma cellulosum 0.15 g of the lichen thallus proved to be sufficient to identify 3 main compounds of lichen substances. Microhydrolysis, EI-masspectroscopy, FD-masspectroscopy and UV/VIS-spectroscopy of the isolated compounds confirm­ ed the presence of perlatolic acid, stenosporic acid, glomelliferic acid and zeorin. 
  Reference    Z. Naturforsch. 33c, 340—345 (1978); eingegangen am 6. Dezember 1977 
  Published    1978 
  Keywords    Nephroma cellulosum, Lichen Acids, Eluchrom, Identification 
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 TEI-XML for    default:Reihe_C/33/ZNC-1978-33c-0340.pdf 
 Identifier    ZNC-1978-33c-0340 
 Volume    33 
63Author    H. Wesch, R. Jonak, H. Nemetschek-Gansler, H. Riedl, Th NemetschekRequires cookie*
 Title    Der Einfluß von D-Penicillamin auf den Gehalt einiger Organe an Spurenelementen sowie auf die mechanischen Eigenschaften von Kollagen Trace Elements in Several Organs and Mechanical Properties of Collagen under the Influence of D-Penicillamin  
 Abstract    The content of trace elements in several organs of rats under the influence of D-penicillamine (D-PA) was investigated by the neutronactivation-analysis. It could be shown an diminution of Cu, and Co under D-PA-treatment, the content of Fe, Mn, Rb and Zn was not influenced. The investigat­ ed organs didn't show any submicroscopic alterations under D-PA. On isolated collagen fibrils of tail tendon was seen a significantly diminuition of E-moduls. In accordance with Siegel the principal effect of D-PA is thought to block the synthesis of functional groups from Schiff-base crosslink precursors but not to inhibit lysyloxidase by loss of Cu-ions of connective tissue. The thermostability of D-PA influenced fibrils is changed in stretched state only and will be due to the lack of crosslink Schiff-bases; where as the shrinking point of not stretched fibrils shows only aging dependent changes. 
  Reference    Z. Naturforsch. 33c, 346 (1978); eingegangen am 17. März 1978 
  Published    1978 
  Keywords    D-Penicillamin, Trace Elements, Electron Microscopy, Biosynthesis, Cross-Links, Mechanical Properties of Collagen 
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 TEI-XML for    default:Reihe_C/33/ZNC-1978-33c-0346.pdf 
 Identifier    ZNC-1978-33c-0346 
 Volume    33 
64Author    Jörg PfistererRequires cookie*
 Title    Messenger Ribonucleoprotein Particles from Plant Cell Cultures  
 Abstract    Plant Cell Cultures, Polysomes, mRNP Particles, mRNA/poly(A) Messenger ribonucleoprotein particles were isolated from the polysomes of logarithmically growing plant cell cultures, pulse-labeled with [3H] adenosine for 30 min. More than 80% of the labeled RNP was present in particles sedimenting between 80 S and 30 S on sucrose density gradients, but was not associated with ribosomal subunits. The size distribution differs from those reported for polysomal mRNP particles to date. After fixation with glutaraldehyde the labeled RNP particles had a buoyant density of 1.38 g/cm3 in CsCl gradients. Radioactively labeled RNA extracted from the RNP particles showed a heterodisperse size distribution and contained poly (A) stretches as determined by affinity chromatography and ribonuclease digestion experiments. 
  Reference    Z. Naturforsch. 33c, 359 (1978); received March 20 1978 
  Published    1978 
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 TEI-XML for    default:Reihe_C/33/ZNC-1978-33c-0359.pdf 
 Identifier    ZNC-1978-33c-0359 
 Volume    33 
65Author    Wolfgang Barz, Rita Schlepphorst, Peter Wilhelm, Westfalen, Karl Kratzl, Erich TenglerRequires cookie*
 Title    Metabolism of Benzoic Acids and Phenols in Cell Suspension Cultures of Soybean and Mung Bean  
 Abstract    Uptake of benzoic and salicylic acid by soybean cell suspension cultures is diffusion-mediated and exclusively leads to glucose-esters. — Veratric acid is para-demethylated to vanillic acid which is conjugated to a monoglucoside. — Nitrobenzoic acids are scarcely taken up by cell cultures with glucose-esters as sole products. — Ring-labelled derivatives of vanillylalcohol and creosol are mainly polymerized to insoluble structures, ring cleavage reactions could not be observed. 
  Reference    Z. Naturforsch. 33c, 363 (1978); received April 12 1978 
  Published    1978 
  Keywords    Benzoic Acids, Phenols, Ring Fission, Peroxidases, Polymerisation, Plant Cell Suspension Cultures 
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 TEI-XML for    default:Reihe_C/33/ZNC-1978-33c-0363.pdf 
 Identifier    ZNC-1978-33c-0363 
 Volume    33 
66Author    Johannes Kösler, Monika Ohm, Wolfgang BarzRequires cookie*
 Title    Metabolism of Anthranilic Acid in Plant Cell Suspension Cultures  
 Abstract    Cell suspension cultures of some 12 plants were investigated for anthranilic acid metabolism. Rapid uptake of substrate is accompanied by partial excretion of anthranilic acid-N-glucoside and followed by predominant conversion into tryptophan. Ring cleavage reactions of anthranilate could not be observed but peroxidatic polymerisation occurred to a high percentage. Anthranilic acid-N-glucoside is not permanently stored by the cell cultures. 
  Reference    Z. Naturforsch. 33c, 368 (1978); received April 19 1978 
  Published    1978 
  Keywords    Anthranilic Acid, Plant Cell Suspension Cultures, Tryptophan, Glucoside, Degradation, Peroxidases 
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 TEI-XML for    default:Reihe_C/33/ZNC-1978-33c-0368.pdf 
 Identifier    ZNC-1978-33c-0368 
 Volume    33 
67Author    R. Stripf, D. WernerRequires cookie*
 Title    Differentiation of Rhizobium japonicum. II. Enzymatic Activities in Bacteroids and Plant Cytoplasm during the Development of Nodules of Glycine max  
 Abstract    Phytotron grown plants of Glycine max var. Caloria infected with Rhizobium japonicum 61-A-101 under controlled conditions as 14 d old seedlings develop a sharp maximum of nitrogenase activity 
  Reference    Z. Naturforsch. 33c, 373—381 (1978); received March 16 1978 
  Published    1978 
  Keywords    Rhizobium japonicum, Glycine max, Bacteroids, Nodules, Nitrogenase 
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 TEI-XML for    default:Reihe_C/33/ZNC-1978-33c-0373.pdf 
 Identifier    ZNC-1978-33c-0373 
 Volume    33 
68Author    H. Heithier, H.-J Galla, H. MöhwaldRequires cookie*
 Title    Fluorescence Spectroscopic and Thermodynamic Studies of Chlorophyll Containing Monolayers and Vesicles. Part I: Mixed Monolayers of Pheophytin A and Lecithin  
 Abstract    Mixed monolayers of pheophytin a and a-L-dimyristoyl lecithin (DML) are investigated on the water surface. The studies gain their special value from the simultaneous measurement of surface pressure and fluorescence intensity as a function of the covered area per molecule. A phase separation in the liquid state of the monolayer is established. Phase 1 exists almost exclusively of pheophytin molecules. Phase 2 exists essentially of D M L domains solubilizing pheo­ phytin in a concentration of 15 mol%. During the D M L main transition the pheophytin solubility decreases to about 2 mol%, the excess pheophytin being precipitated within the surface layer. During the pheophytin main transition an ordered structure below the surface layer is formed. A stabilizing interaction between the pheophytin domains and their environment is observed and discussed. A sharp fluorescence change at a pressure below 0.5 dyn/cm indicates another phase transition. It very probably involves an unwrapping of pheophytin from water molecules. 
  Reference    Z. Naturforsch. 33c, 382 (1978); received March 15/April 3 1978 
  Published    1978 
  Keywords    Monolayers, Chlorophyll, Energy Transfer, Fluorescence Spectroscopy, Phase Transitions 
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 TEI-XML for    default:Reihe_C/33/ZNC-1978-33c-0382.pdf 
 Identifier    ZNC-1978-33c-0382 
 Volume    33 
69Author    Wolfgang Haehnel, Adelheid Heupel, Dorothea HengstermannRequires cookie*
 Title    Investigations on a Galvanic Cell Driven by Photosynthetic Electron Transport  
 Abstract    A light-driven galvanic cell was constructed making use of the photosynthetic activity of isolated chloroplasts. Artificial mediators managed the transfer of electrons from the endogenous electron carriers to the platinum electrodes in each of the joined half-cells. In one the mediators were reduc­ ed by electrons originating from water. In the other the mediators were oxidized by photosystem I in the presence of an autoxidizable electron acceptor. The redox potential in the single half-cells has been studied as a function of the lipophilicity of the mediators and their concentration. Further­ more different autoxidizable acceptors and different treatments of the chloroplasts were investigated. The combined half-cells were separated by an ultrafiltration membrane. Upon illumination the system gave rise to an open circuit potential of up to 220 mV. This battery was charged with rates as high as photosynthetic electron transport rates. The results are discussed with respect to the arrangement of the cell and the properties of the components for high effectiveness and maximal potential differences. 
  Reference    Z. Naturforsch. 33c, 392 (1978); received March 23 1978 
  Published    1978 
  Keywords    Photosynthetic Electron Transport, Artificial Mediators, Galvanic Cell, Solar Energy Conversion 
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 TEI-XML for    default:Reihe_C/33/ZNC-1978-33c-0392.pdf 
 Identifier    ZNC-1978-33c-0392 
 Volume    33 
70Author    Christian HolzapfelRequires cookie*
 Title    Analysis of the Prompt Fluorescence Induction by Means of Computer Simulation of the Primary Photosynthetic Reactions  
 Abstract    The induction phenomenon of prompt fluorescence of the photosynthetic system in green plants reflects the adaptation of the electron transport system from a dark adapted state to a steady state under light condition. The appearance of the P-peak in the prompt fluorescence induction curve is explained by the decay of the electrochemical gradient across the thylakoid membrane in the dark. The decay of the electrochemical gradient in the dark is calculated using measured fluorescence induction curves with different times of dark adaptation. The influence of photophosphorylation inhibitors is discussed and the threshold effect of ATP-formation depending on the electrochemical gradient is explained by the coupling of the ATP-formation with the electron transport in the thylakoid membrane. 
  Reference    Z. Naturforsch. 33c, 402—2 (1978); received April 17 1978 
  Published    1978 
  Keywords    Primary Photosynthetic Electron Transport, Computer Simulation, Fluorescence Induction, ATP-Formation 
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 TEI-XML for    default:Reihe_C/33/ZNC-1978-33c-0402.pdf 
 Identifier    ZNC-1978-33c-0402 
 Volume    33 
71Author    Siegrid Schoch, Wolfram SchäferRequires cookie*
 Title    Tetrahydrogeranylgeraniol, a Precursor of Phytol in the Biosynthesis of Chlorophyll a — Localization of the Double Bonds  
 Abstract    Pheophytins esterified with phytol and tetrahydrogeranylgeraniol are isolated from etiolated oat seedlings after short (1 min) exposure to light and a subsequent dark period of 15 to 20 min. After saponification of the pheophytins, a mixture of the alcohols was isolated. The structure of tetra­ hydrogeranylgeraniol was established as 3,7,11,15-tetramethyl-zl2'14 hexadecadiene-l-ol (3a). The implications for chlorophyll biosynthesis are discussed. 
  Reference    Z. Naturforsch. 33c, 408—412 (1978); received March 23 1978 
  Published    1978 
  Keywords    Birthday Avena sativa, Gramineae, Oats, Chlorophyll Biosynthesis, Phytol 
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 TEI-XML for    default:Reihe_C/33/ZNC-1978-33c-0408.pdf 
 Identifier    ZNC-1978-33c-0408 
 Volume    33 
72Author    J.J S Van Rensen, D. Wong, GovindjeeRequires cookie*
 Title    Characterization of the Inhibition of Photosynthetic Electron Transport in Pea Chloroplasts by the Herbicide 4,6-Dinitro- o-cresol by Comparative Studies with 3-(3,4-Dichlorophenyl)-l,l- dimethylurea  
 Abstract    An attempt to characterize the mechanism of inhibition of photosynthetic electron transport in isolated pea chloroplasts by the herbicide 4,6-dinitro-o-cresol (DNOC) by a comparison with the effects of 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) revealed the following: 
  Reference    Z. Naturforsch. 33c, 413 (1978); received April 12 1978 
  Published    1978 
  Keywords    Photosynthesis, Electron Transport, Fluorescence, Herbicides 
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 TEI-XML for    default:Reihe_C/33/ZNC-1978-33c-0413.pdf 
 Identifier    ZNC-1978-33c-0413 
 Volume    33 
73Author    Peter Gräber, Sathamm SaphonRequires cookie*
 Title    Conformational Changes of the Membrane-Bound ATPase of Bacterial Chromatophores Revealed by Fluorescence Changes of Fluorescamine-Labelled Coupling Factors  
 Abstract    The solubilized coupling factor (Ft) of Rps. sphaeroides chromatophores was allowed to react with fiuorescamine which led to a fluorescence labelled F t . After reconstitution with the depleted membranes the fluorescence-labelled Fj was shown to restore photophosphorylation in continuous light in a similar way to the non-labelled F t . In parallel, a decrease of the fluorescence emission of the labelled and reconstituted coupling factor was observed. The solubilized and labelled F x showed also a fluorescence decrease as the polarity of the medium was increased. In single turnover flashes the fluorescence change was found to be inhibited by an uncoupling agent such as FCCP. The kinetics of the change were sensitive to phosphorylating agents and to an "energy transfer inhibitor" such as venturicidin. It is suggested that the observed fluorescence changes reflect conformational changes of the ATPase enzyme complex. 
  Reference    Z. Naturforsch. 33c, 421—427 (1978); received March 28 1978 
  Published    1978 
  Keywords    Chromatophores Coupling Factors, Fluorescence-Labelling, Conformational Changes 
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 TEI-XML for    default:Reihe_C/33/ZNC-1978-33c-0421.pdf 
 Identifier    ZNC-1978-33c-0421 
 Volume    33 
74Author    B. Agostini, M. Stromer, W. HasselbadiRequires cookie*
 Title    Effect of Lipid Modification on Fusion of Sarcoplasmic Reticulum Vesicles  
 Abstract    Freeze fracture technique ascertains that sarcoplasmic reticulum vesicles fuse during aniso-diametric dehydration which leads to the formation of sheetlike structure o f a mean extension of 5000 A . M odification of the membrane lipids by phospholipase A 2 digestion or the incorporation of deoxycholate facilitates the coalescence of the vesicles, while it is com pletely prevented by lipid removal. Membrane fusion during anisodiametric dehydration is considered as resulting from the close contact of highly curved edges from which the 
  Reference    Z. Naturforsch. 33c, 428 (1978); received A p ril 11 1978 
  Published    1978 
  Keywords    Membrane Fusion, Sarcoplasmic Reticulum, Freeze Fracturing, Anisometric Dehydration, L ipid Modification 
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 TEI-XML for    default:Reihe_C/33/ZNC-1978-33c-0428.pdf 
 Identifier    ZNC-1978-33c-0428 
 Volume    33 
75Author    Paul Kuppert, M. Argit Büchler, Klaus-Dieter SpindlerRequires cookie*
 Title    Distribution and Transport of Molting Hormones in the Crayfish, Orconectes limosus  
 Abstract    W hen [ aH ]ecdysone is injected in intermolt crayfishes, about 60 — 70% of the total radioactivity are excreted within one hour as the unchanged hormone, but nearly no [ 3H]ecdysterone is ex­ creted. The remaining [ 3H]ecdysone in the body is effectively converted to [ 3H ] ecdysterone. In addition polar and apolar metabolites of both m olting hormones can be extracted in an organ specific pattern. On the basis of radioactivity per g fresh weight hypodermis and male gonads and to a lesser extent hindgut and midgut gland contain most of the injected radioactivity. It can be demonstrated from in vitro experiments using the charcoal adsorption test and equilibrium dialysis that the cytosol of target tissues is able to bind both hormones with the highest capacity for hypo­ dermis and male gonads. Both molting hormones do not appear to be bound to carrier proteins in the hemolymph. 
  Reference    Z. Naturforsch. 33c, 437 (1978); received A p ril 24 1978 
  Published    1978 
  Keywords    M olting Hormones, Crayfish, Ecdysteroid Tissue Distribution, Ecdysteroid Hemolymph Transport 
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 TEI-XML for    default:Reihe_C/33/ZNC-1978-33c-0437.pdf 
 Identifier    ZNC-1978-33c-0437 
 Volume    33 
76Author    G. Uhlenbruck, B. P. Chatterjee, U. SchuldesRequires cookie*
 Title    Schiff Base Form ation: Dem onstrated by a Precipitin Reaction in A g a r  
 Abstract    High-m olecular weight polyaldehydes, which can be o b ­ tained by periodate oxidation of glycosubstances, especially polysaccharides, do precipitate in a specific way by Schiff base formation, when diffusing in agar gel against high-m olecular weight, naturally occurring polyamines. album (m istletoe), R icinus com m unis and from the bivalve clam Tridacna m axim a (see Fig. l a) . Also the plant lectins from Ononis spinosa and Abrus precatorius do give such a precipitin reaction. This precipitation phenomenon however is abolished, when the terminal D-galactose (/?-l-6 linked) has been oxidized by sodium periodate (F ig . l b) , whereas the precipitin reaction due to Schiff base form ation does not occur with the genuine, un­ treated arabinogalactan (F ig . 1 a) . N egative reac­ tions are also seen after borohydride reduction or by blocking the carbonyl groups (o r the amino groups) by other methods [ 1 1 ] *. Oxidation o f lymphocytes from a variety of spe­ cies with either sodium periodate, galactose-oxidase or neuraminidase and galactose-oxidase induces blastogenic transformation [ 1 — 5 ], or lymphocyte cytotoxicity [ 6]. In addition, tumor cell killing occurs, when by the same procedures artificial con­ tacts are established between normal macrophages and tumor cells [ 7 ]. Although these intriguing phenomena are not yet completely elucidated, it is assumed that cross-linkages via Schiff bases play an important role [6 — 8]. The only evidence for this hypothesis is given by the fact, that sodium b oro­ hydride treatment abolishes the effect. It was there­ fore desirable, to have an in v itro model for the form ation o f Schiff bases in order to study the inter­ action o f different membrane glycosubstances and proteins. A s standard materials for developing a representative precipitin experiment we have first chosen periodate-treated arabinogalactan from plant origin [9 ] and polylysine as amino group donator [10]. In Fig. 1 it is demonstrated, that Schiff base fo r ­ mation between these two components indeed can be made visible in form o f a precipitation line. In addition, b iological substances with free amino groups, like histone [ 10], lysozyme [ 10] and haem oglobin [1 1 ] react in a similar way (F ig . 1 b), while a synthetic basic polym er without any amino groups, namely polybrene [ 10], does not pre­ cipitate as has been found by us. On the other hand, genuine arabinogalactan precipitates with some anti-galactosyl specific lectins like the one from Viscum 
  Reference    Z. Naturforsch. 33c, 442 (1978); received February 2 1978 
  Published    1978 
  Keywords    Basic Polyam inoacids Periodate-Treatment, Arabino-galactan, Lectins, Cell Aggregation 
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 TEI-XML for    default:Reihe_C/33/ZNC-1978-33c-0442_n.pdf 
 Identifier    ZNC-1978-33c-0442_n 
 Volume    33 
77Author    Läuchli, A. SieversRequires cookie*
 Title     
 Abstract    A p ril 5, 1978 X -R a y M icroanalysis, Barium and Calcium Determination, Statolith Composition, Characean C ell W a ll 
  Reference    Z. Naturforsch. 33c, 444 (1978); received 
  Published    1978 
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 TEI-XML for    default:Reihe_C/33/ZNC-1978-33c-0444_n.pdf 
 Identifier    ZNC-1978-33c-0444_n 
 Volume    33 
78Author    Arindra Singh, Satyavan Sharma, R. N. Iyer, A. B. SenRequires cookie*
 Title     
 Abstract    Cestodicidal Agent, H y m e n o le p is nana 2'-Chloro-l-hydroxy-2-naphthanilide-4'-isothiocyanate (4) has been synthesized as the structural analogue of yomesan (1) and was found to be active against experimental dw arf 
  Reference    Z. Naturforsch. 33c, 447 (1978); received M arch 9 1978 
  Published    1978 
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 TEI-XML for    default:Reihe_C/33/ZNC-1978-33c-0447_n.pdf 
 Identifier    ZNC-1978-33c-0447_n 
 Volume    33 
79Author    Y., E. Solberg, Gabriele RemediosRequires cookie*
 Title    Studies on the Chem istry o f Lichens, X V I I I  
 Abstract    Chem ical Investigation o f the Species Letharia vulpina (L .) H u e and N e w D erivatives o f V u lp in ic A c id Lichen L e th a ria v u lp in a , Vulpinic A c id Derivatives T he crystalline and amorphous constituents of Leth a ria vu lp in a collected at Flensjeen, Sor-Trondelag, N orway, have been identified as atranorin, vulpinic-, pulvinic-, usnic-, benzoic-and hydroxyfatty acids. Vulpinic acid th allate(I) and vulpinic acid isopropylether have been prepared as new derivatives. The identity of the compounds was established by consideration of elemental analyses, infrared, mass and nuclear magnetic resonance spectra, and thin layer chro­ m atography. In tro d u ctio n Th e study o f the lichen species Letharia vulpina (L .) Hue has been carried out by several groups o f investigators, and vulpinic acid was reported to be the principal chemical constituent. Atranorin is for the first time isolated and described from this lichen by Hesse as early as 1898 [ 1 ] . H e established that vulpinic acid was always accompanied by atranorin in Letharia vulpina. In a work by Stahl and Schorn [2 ] on T L C of, among other items, lichen sub­ stances, usnic acid is mentioned as a component o f lichen species. Bachelor and Cheriyan [3 ] investi­ gated the species and reported the occurrence of free benzoic acid. Pulvinic acid or other derivatives o f this compound have not as yet been reported from Letharia vulpina. T h e purpose o f the present work is to report the results o f a chemical re-examination o f the lichen Letharia vulpina subjected at our institute. In agree­ ment with existing literature atranorin, vulpinic-, usnic-and benzoic acid were found as the m ajor constituents. It is o f interest that pulvinic acid, too was found to occur in our N orw egian sample of Letharia vulpina. A n amorphous mass was isolated and established as a mixture o f high molecular hy­ droxyfatty acids. The 2-aminoethanesulfonic acid taurine was detected in a water extract by means o f 
  Reference    Z. Naturforsch. 33c, 449 (1978); received January 23/April 3 1978 
  Published    1978 
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 TEI-XML for    default:Reihe_C/33/ZNC-1978-33c-0449_n.pdf 
 Identifier    ZNC-1978-33c-0449_n 
 Volume    33 
80Author    Hans-Henning SommerRequires cookie*
 Title    on Stimulus Duration under Speedy Light-D ark Cycles  
 Abstract    L/D-Reactions, B a la n u s balanus, Activity per U nit Time, Stimulating/Inhibitory Effects 
  Reference    Z. Naturforsch. 33c, 452 (1978); eingegangen am 22. März/17. A p ril 1978 
  Published    1978 
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 TEI-XML for    default:Reihe_C/33/ZNC-1978-33c-0452_n.pdf 
 Identifier    ZNC-1978-33c-0452_n 
 Volume    33 
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