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1991 (171)
61Author    H. Schäfer, K. Taraz, H. BudzikiewiczRequires cookie*
 Title    Zur Genese der amidisch an den Chromophor von Pyoverdinen gebundenen Dicarbonsäuren |1] On the Genesis of the Dicarboxylic Acids Bound Amidically to the C hrom ophor of the Pyoverdins [1]  
 Abstract    Pseudomonas strains o f the so-called fluorescent group usually produce several pyoverdins which differ only in the nature o f a dicarboxylic acid bound amidically to the chromophor. For the pyoverdins isolated from the culture medium o f Pseudomonas fluorescens 12 it is shown that succinic acid is an artefact formed by hydrolysis o f succinic amide, and that a-ketoglutaric acid is transformed enzymatically to glutamic acid. This process is reversed after the phase o f exponential growth o f the bacteria. The ratio C4-vs. C 5-acids changes with the culture time and with increasing Fe3+ content o f the medium in favor o f the latter. 
  Reference    Z. Naturforsch. 46c, 398 (1991); received December 21 1990/March 13 1991 
  Published    1991 
  Keywords    *Pyoverdins, Biogenesis, Transformations, Citric Acid Cycle, Pseudomonas fluorescens 
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 TEI-XML for    default:Reihe_C/46/ZNC-1991-46c-0398.pdf 
 Identifier    ZNC-1991-46c-0398 
 Volume    46 
62Author    SuvendraN. Bagchi, Anneliese Ernst, Peter BögerRequires cookie*
 Title    The Effect of Activated Oxygen Species on Nitrogenase of Anabaena variabilis  
 Abstract    Hydrogen Peroxide, Superoxide, M ethylviologen, Nitrogenase M odification and Destruction The heterocyst-forming cyanobacterium Anabaena variabilis (ATCC 29413) is deficient in catalase activity, but especially heterocysts are rich in superoxide dismutase and peroxidases. It exhibits a light-dependent methylviologen-catalyzed oxygen uptake which is accompanied by a massive release o f hydrogen peroxide. M ethylviologen caused a transient concentration-dependent inhibition o f nitrogenase ending in a complete loss o f activity. Suppression o f hy­ drogen peroxide accumulation by the photosynthesis inhibitor D C M U (diuron, 3-[3,4-dichlo-rophenyl]-l,l-dim ethylurea), or by addition o f catalase prevented nitrogenase inhibition indi­ cating that methylviologen affects nitrogenase activity via hydrogen peroxide produced by photosynthetic electron transport. Immunospecific Western blotting o f cell extracts separated on SDS polyacrylamide gel electrophoresis showed that the transient inhibition o f nitrogenase is accompanied by rapid modification o f its Fe-protein followed by enzyme destruction. In a cell-free system the enzyme exhibited only moderate sensitivity towards hydrogen peroxide but was found susceptible against activated oxygen species generated by the xanthine/xanthine oxidase system and free iron. The results indicate that H-,0: itself has little direct effect on nitro­ genase, but endogenous peroxide produced by methylviologen in the light may initiate oxygen radical formation leading to nitrogenase m odification and subsequently its destruction. 
  Reference    Z. Naturforsch. 46c, 407 (1991); received January 18 1991 
  Published    1991 
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 TEI-XML for    default:Reihe_C/46/ZNC-1991-46c-0407.pdf 
 Identifier    ZNC-1991-46c-0407 
 Volume    46 
63Author    G. Ruyters, N. G. Rotjohann, W. KowallikRequires cookie*
 Title    Oligomeric Forms of Pyruvate Kinase from Chlorella with Different Kinetic Properties  
 Abstract    Chlorella kessleri, Enzyme R egulation, FPLC Superose 6 Separation, Oligomerization. Pyru­ vate Kinase Fast protein liquid chromatography on Superose 6 o f crude extracts from the chlorophyll-free mutant no. 20 o f the unicellular green alga Chlorella kessleri reveals two possibly o lig o ­ meric forms o f pyruvate kinase (2.7.1.40). Their occurrence is markedly altered in the course o f heterotrophic growth with changing levels o f exogenous glucose as carbon source with only one enzyme species with a MW o f 400 kDa existing in growing cells, two forms o f 400 and 580 kD a in resting cells. Substrate affinity towards PEP o f the 400 kDa form is better than that o f the 580 kD a species; responses to the effector A M P are different as well. In vitro, addition o f PEP or o f A M P leads to the formation o f higher MW enzyme species with MW o f 730, 1050 and 1400 kD A without affecting the total activity. In vivo alterations in the levels o f several m etabolites including PEP upon addition o f glucose have been shown to occur. Therefore, it is discussed, whether changes in the concentration o f intermediates and effectors may provide the mechanism for the increased rate o f carbohydrate degradation by affecting the occurrence and/or ratio o f the various PK forms with different kinetic and regulatory properties. U pon blue light irradiation, which also stimulates carbohydrate breakdown o f the Chlorella mutant cells, the distribution o f PK is shifted towards the species with higher substrate affinity, a re­ sult being in accordance with the above conception. 
  Reference    Z. Naturforsch. 46c, 416 (1991); received January 31/March 5 1991 
  Published    1991 
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 TEI-XML for    default:Reihe_C/46/ZNC-1991-46c-0416.pdf 
 Identifier    ZNC-1991-46c-0416 
 Volume    46 
64Author    AndrzejB. HendrichRequires cookie*
 Title    Calorimetric Study on the Interactions of 5-/i-Heptadec(en)yIresolcinols from Cereal Grains with Zwitterionic Phospholipid (D PPC )  
  Reference    Z. Naturforsch. 46c, 423 (1991) 
  Published    1991 
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 TEI-XML for    default:Reihe_C/46/ZNC-1991-46c-0423.pdf 
 Identifier    ZNC-1991-46c-0423 
 Volume    46 
65Author    Tibor Cserhá, ZoltáN. Szigeti1Requires cookie*
 Title    Ions Modify the Strength of Interaction of Diquat and Paraquat with Some Proteins and Cellulose  
 Abstract    entral R esearch In stitu te o f C hem istry. H u ngarian A cadem y o f Sciences. H-1525 B udapest. P.O .B. 17, H ungary 1 D ep artm en t o f Plant Physiology. Eötvös U niversity, H-1445 B udapest. P.O .B. 330. H ungary Z. N aturforsch . 46c, 4 2 8 -4 3 2 (1991); received Jan u ary 18/F ebruary 26, 1991 D iquat, P a ra q u a t. Bipyridyles, G liadin. H um an Serum A lbum in, M ono-and D ivalent C ations The interactio n o f the bipyridylium herbicides d iq u at and p a ra q u a t w ith the p roteins gliadin and hum an serum album in (H SA) as well as with cellulose and the effect o f various ions on the strength o f interactio n were studied by ch arge-transfer ch ro m ato g rap h y . Both com p o u n d show ed very sim ilar behaviour. The ions decreased in each case the stren g th o f interaction, the effect depended nonlinearly on the ion concen tratio n and was o f sa tu ra tio n ch aracter. G liadin show ed the w eakest in teraction. HSA binds the bipyridylium co m p o u n d s in an ion-dependent m anner, the strength o f in teraction is higher than th at o f cellulose at higher ion co n cen tratio n . T his finding indicates an ion-m ediated interaction o f unknow n ch a ra c ter betw een the b ipyri­ dylium herbicides and HSA. 
  Reference    Z. Naturforsch. 46c, 428 (1991) 
  Published    1991 
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 Identifier    ZNC-1991-46c-0428 
 Volume    46 
66Author    Requires cookie*
 Title    Slit Scan Flow Cytometry of Isolated Chromosomes Following Fluorescence Hybridization: an Approach of Online Screening for Specific Chromosomes and Chromosome Translocations  
  Reference    Z. Naturforsch. 46c, 433 (1991) 
  Published    1991 
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 Identifier    ZNC-1991-46c-0433 
 Volume    46 
67Author    G.Erhard Erkel, Timm AnkeRequires cookie*
 Title    Podoscyphic Acid, a New Inhibitor of Avian Myeloblastosis Virus and Moloney Murine Leukemia Virus Reverse Transcriptase from a Podoscypha Species [1]  
  Reference    Z. Naturforsch. 46c, 442 (1991) 
  Published    1991 
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 Identifier    ZNC-1991-46c-0442 
 Volume    46 
68Author    Peter HochstrateRequires cookie*
 Title    Electrogenic N a+-C a 2+ Exchange Contributes to the Light Response of Fly Photoreceptors  
 Abstract    In stitu t für Tierphysiologie, R u h r-U n iv ersität, Postfach 102 148, D-4630 B ochum 1, B undesrepublik D eutschland Z. N aturforsch. 46c, 451 -4 6 0 (1991); received O ctober 25, 1990/F ebruary 15, 1991 V ision, P hotoreceptor, Fly, N a +-C a 2+ E xchange, E lectrophysiology T he response o f fly photo recep to rs to light stim uli o f high intensity consists o f a fast tra n ­ sient depolarization caused by the activation o f light-dependent channels and a delayed a fter­ depolarization. The afterdepolarization was reversibly reduced o r abolished w hen the extracel­ lular N a + had been replaced by L i+, tris o r choline. N a + specificity, the dependence on the con cen tratio n s o f N a + and C a2+ as well as the p o larity o f the effect suggest th a t electrogenic N a +-C a 2+ exchange contrib u tes to the afterd ep o larizatio n . Prolonged in cu b atio n in N a +-free m edia also led to the reversible suppression o f the fast response co m p o n en t, w hich is m ost p ro b ab ly due to an increase in the intracellular C a2+ co n cen tratio n caused by the inhibition o f N a + —C a 2+ exchange. Evidence is presented th a t the N a +-C a 2+ exchange system in fly p h o to ­ receptors also transp o rts Sr2+. 
  Reference    Z. Naturforsch. 46c, 451 (1991) 
  Published    1991 
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 Identifier    ZNC-1991-46c-0451 
 Volume    46 
69Author    H. Stieve, H. Reuß, H. T. Hennig, J. KlomfaßRequires cookie*
 Title    Single Photon-Evoked Events of the Ventral Nerve Photoreceptor Cell of Limulus Facilitation, Adaptation and Dependence of Lowered External Calcium  
  Reference    Z. Naturforsch. 46c, 461 (1991) 
  Published    1991 
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 TEI-XML for    default:Reihe_C/46/ZNC-1991-46c-0461.pdf 
 Identifier    ZNC-1991-46c-0461 
 Volume    46 
70Author    Piotr Pawlowski, Magdalena FikusRequires cookie*
 Title    Shear Deformation of the Spherical Shell Acted on by an External Alternating Electric Field: Possible Applications to Cell Deformation Experiments  
 Abstract    Shape deformation of the thin, area non-compressible spherical shell separating two differ­ ent media, subjected to an external, alternating, homogeneous electric field is considered. The function relating shape deformation of the shell to the shear stress in its plane is presented. Shear stress develops as a result of Maxwell stress acting on both shell surfaces. All media are considered as lossy. The theoretical model predicts that the shear stress reaches its maximal value at the shell "equator" in relation to the field direction. The magnitude of the shear stress depends on the strength and frequency of the electric field, on the geometric parameters of the shell, and on the electric parameters of the system studied. The foregoing was applied to an analysis of cell deformation, e.g., an erythrocyte in an elec­ tric field. The analysis predicts that the maximal shear stress in the membrane develops at field frequencies close to f = 107 Hz. This is comparable to the electrocompression stress of the same membrane, maximal at the cell "pole". At lower frequencies, compression predominates over shear stress. 
  Reference    Z. Naturforsch. 46c, 487—494 (1991); received March 6 1990/ 
  Published    1991 
  Keywords    Periodic Electric Field, Spherical Shell Rheology, Shear Deformation, Shear Elastic Modulus, Electrical Parameters 
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 TEI-XML for    default:Reihe_C/46/ZNC-1991-46c-0487.pdf 
 Identifier    ZNC-1991-46c-0487 
 Volume    46 
71Author    Eckhard Wollenweber, Karin Mann, JamesN. RoitmanRequires cookie*
 Title    Flavonoid Aglycones from the Bud Exudates of Three Betulaceae  
 Abstract    Ainus koelmei, Betula nigra, O strva carpinifolia, Bud Exudates, Flavonoid Aglycones Bud Exudates of three Betulaceae were analyzed for minor flavonoid aglycones. Three flavonoids not pre­ viously reported were found in Alnus koehnei, four in Betula nigra. A novel flavonol, morin-3,2',4'-trimethyl ether, is described for O strya carpinifolia. 
  Reference    Z. Naturforsch. 46c, 495—497 (1991); received February 11 1991 
  Published    1991 
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 TEI-XML for    default:Reihe_C/46/ZNC-1991-46c-0495_n.pdf 
 Identifier    ZNC-1991-46c-0495_n 
 Volume    46 
72Author    K.-H Glüsenkamp, W. Drosdziok, G. Eberle, E. Jähde, M. F. RajewskyRequires cookie*
 Title    Squaric Acid Diethylester: A Simple and Convenient Coupling Reagent  
 Abstract    A new method for the covalent modification of poly­ mers in a two-step process is described. Squaric acid di­ ethylester reacts with primary or secondary aliphatic amines selectively to ester amides. It is shown for the first time that this class of compounds react with poly­ meric systems containing amine functionalities (e.g. g-amino groups of proteins) in aqueous solution, form­ ing stable squaric acid diamide linkages. Three different monoamides were synthesized and the reaction rates of diamide formation versus hydrolysis were determined. Detailed kinetic studies revealed that unwanted hydroly­ tic side-reactions are more than ten times slower than the formation of squaric acid diamides. 
  Reference    Z. Naturforsch. 46c, 498—501 (1991); received March 61991 
  Published    1991 
  Keywords    Squaric Acid Diethylester, Squaric Acid Monoamides, Squaric Acid Diamides, Bifunctional Coupling Reagent, Covalent Modification 
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 Identifier    ZNC-1991-46c-0498_n 
 Volume    46 
73Author    Hartwig Hohlfeld1 ', Cornelius Lützb, Dieter StrackaRequires cookie*
 Title    Phosphoenolpyruvate Carboxylase Activity in Norway Spruce Needles: Effects of Air Pollutants under Controlled Conditions  
 Abstract    The effect of mixtures of air pollutants (S 02, N 0 2, O,) on phosphoenolpyruvate carboxylase (PEPCase; EC 4.1.1.31) activities from needles of four-year-old Norway spruce (Picea abies [L.] Karst.) in closed fumi­ gation chambers were analyzed. Three months exposure to high concentrations of 0 3 (1030 |ig/m3) together with S 0 2 (1030 |ig/m3) or N 0 2 (615 |ig/m?) resulted in a dra­ matic increase in PEPCase activities. 
  Reference    Z. Naturforsch. 46c, 502—505 (1991); received January 25/March 51991 
  Published    1991 
  Keywords    Picea abies, Phosphoenolpyruvate Carboxylase (PEPCase), Forest Decline, Air Pollutants, Phytotron 
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 Identifier    ZNC-1991-46c-0502_n 
 Volume    46 
74Author    F. J. Arriaga-Giner, J. Rullkötter, T. M. Peakman, E. WollenweberRequires cookie*
 Title    New Triterpenes from the Frond Exudate of the Fern Notholaena vigida  
 Abstract    Two new triterpenoids, isolated from the frond exudate of the fern Notholaena rigida, have been identified as 3 ß, 12 ß,25-trihydroxy-(20/?-24/?)-epoxy-dammarane-12-acetate (1), and 24/?,25-diO-isopropyliden-9(19)-cyclolanostan-3 ß-ol (5), based on N M R spectroscopic studies. 
  Reference    Z. Naturforsch. 46c, 507—512 (1991); received December 28 1990/April 4 1991 
  Published    1991 
  Keywords    Notholaena rigida, Pteridaceae, Farinose Frond Exudate, Triterpenes, Novel Natural Products 
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 Identifier    ZNC-1991-46c-0507 
 Volume    46 
75Author    Yusuke Shibuya, Satoshi Tahara, Yutaka Kimura, Junya MizutaniRequires cookie*
 Title    New Isoflavone Glucosides from White Lupin (Lupinus albus L.)  
 Abstract    Further investigation of polar constituents in white lupin roots revealed the presence of genistein 7-O-ß-glucoside (la), genistein 4'-0-ß-glucoside (lb), 2'-hydroxygenistein 7-0-ß-glucoside (2a) and 2'-hydroxygenistein 4'-0-ß-glucoside (2b) as simple isoflavone gluco­ sides; luteone 7-O-ß-glucoside (3 a), licoisoflavone A 7-O-ß-glucoside (4 a) and licoisoflavone A 4'-0-ß-glucoside (4b) as prenylated (= 3,3-dimethylallyl-substituted) isoflavone glucosides; and genistein 7-0-ß-(6"-0-malonyl)glucoside (lam) and 2'-hydroxygenistein 7-0-ß-(6''-0-malonyl)glueoside (2 am) as malonylated isoflavone glucosides. Six compounds (2 a, 2 b, 3 a, 4a, 4b, and 2am) are new naturally occurring isoflavone glucosides. 
  Reference    Z. Naturforsch. 46c, 513—518 (1991); received February 12/May 2 1991 
  Published    1991 
  Keywords    Isoflavonoids, Malonylglucosides, White Lupin, Lupinus albus, Leguminosae 
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 Identifier    ZNC-1991-46c-0513 
 Volume    46 
76Author    Katja Doerk3, Ludger Witteb, A.Wilhelm Alfermann3Requires cookie*
 Title    Identification of Tropane Alkaloids in Hairy Root Cultures of Hyoscyamus albus  
 Abstract    D edicated to Professor Ernst Reinhard on the occasion o f his 65 th birthday GC-MS, Hairy Roots, Hyoscyamus albus, Littorine, Tropane Alkaloids Tropane alkaloids are typical natural products of solanaceous plants. The patterns of these alkaloids from hairy roots of Hyoscyamus albus were determined by GC-MS analysis. 18 alka-loidal compounds were detected, six of them only in trace amounts. Some of these alkaloids, namely hygrine, 3a-acetoxytropane, 3 ß-acetoxytropane and 6-hydroxylittorine as well as the trace compounds N-methylpyrrolidinylhygrine, phenylacetoxytropane and two isomers of feruloyloxytropane were not previously reported to be components of this species. The major compounds formed were found to be hyoscyamine and littorine. 
  Reference    Z. Naturforsch. 46c, 519—521 (1991); received December 13 1990/April 9 1991 
  Published    1991 
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 Identifier    ZNC-1991-46c-0519 
 Volume    46 
77Author    K. Taraz, D. Seinsche, H. BudzikiewiczRequires cookie*
 Title    Pseudobactin-und Pseudobactin A-Varianten: Neue Peptidsiderophore vom Pyoverdin-Typ aus Pseudomonas fluovescens "E2" [1] Variants of Pseudobactin and Pseudobactin A: New Pyoverdin Type Peptide Siderophores from Pseudomonas fluorescens "E 2"  
 Abstract    From a strain of Pseudomonas fluorescens pseudobactin and several related compounds were isolated and their structures were elucidated. In this way a reference compound (5) could be obtained for the unambiguous determination of the absolute configuration of C-l of the pyoverdin chromophore in newly isolated representatives of this class. 
  Reference    Z. Naturforsch. 46c, 522—526 (1991); received February 20/April 9 1991 
  Published    1991 
  Keywords    Pseudomonas fluorescens, Siderophores, Pyoverdins, Pseudobactin, Structure Elucidation 
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 TEI-XML for    default:Reihe_C/46/ZNC-1991-46c-0522.pdf 
 Identifier    ZNC-1991-46c-0522 
 Volume    46 
78Author    K. Taraz, R. Tappe, H. Schröder, U. Hohlneicher, I. Gwose, H. Budzikiewicz, G. Mohn, J. F. LefèvreRequires cookie*
 Title    Ferribactins -the Biogenetic Precursors of Pyoverdins [1]  
 Abstract    From ferribactins which accompany pyoverdins in iron deficient cultures of fluorescent pseudomonads a subunit has been obtained formed by condensation of D-tyrosin with L-2,4-diaminobutyric acid to give 2-(l-./?-amino-2-/?-hydroxyphenylethyl)-1,4,5,6-tetrahydro-pyrimidine-4-S'-carboxylic acid (1). Evidence is presented that 1 is the precursor of the typical pyoverdin chromophore 7 a. 
  Reference    Z. Naturforsch. 46c, 527—533 (1991); received February 28 1991 
  Published    1991 
  Keywords    Pyoverdins, Ferribactins, Biogenesis, N M R Analysis 
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 Identifier    ZNC-1991-46c-0527 
 Volume    46 
79Author    S. Gipp, J. Hahn, K. Taraz, H. BudzikiewiczRequires cookie*
 Title    Zwei Pyoverdine aus Pseudomonas aeruginosa R. [1] Two Pyoverdins from Pseudomonas aeruginosa R  
 Abstract    Pseudomonas aeruginosa, Pyoverdins The structure elucidation of two pyoverdins from the strain R. of Pseudomonas aeruginosa is described. 
  Reference    Z. Naturforsch. 46c, 534—541 (1991); received April 10 1991 
  Published    1991 
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 Identifier    ZNC-1991-46c-0534 
 Volume    46 
80Author    F. López-FigueroaRequires cookie*
 Title    Control by Light Quality of Chlorophyll Synthesis in the Brown Alga Desmarestia aculeata  
 Abstract    The chlorophyll synthesis in the brown algae D esm arestia aculeata is affected by light quali­ ty and by the nutrient state in the medium before the illumination. Pulses of 5 min of red, green and blue light together with 200 |iM nitrate in plants growing under natural conditions deter­ mined similar induction of chlorophyll synthesis. However, when the plants were incubated previously under starvation conditions the light effect was different. The induction of chloro­ phyll synthesis was greater after blue and green light than after red light pulses. Red-light pho­ toreceptor was only involved in the chlorophyll synthesis under no nutrient limitations and under starvation conditions after previous illumination with blue light followed by far-red light. The induction of chlorophyll synthesis by green and blue light pulses applied together with nitrate was greater when the algae were incubated in starvation conditions than in natural conditions (normal nutrient state). Because all light effects were partially reversed by far-red light the involvement of a phyto-chrome-like photoreceptor is proposed. In addition, a coaction between blue-and a green-light photoreceptors and phytochrome is suggested. 
  Reference    Z. Naturforsch. 46c, 542—548 (1991); received February 13 1991 
  Published    1991 
  Keywords    Blue-Light Photoreceptor, Chlorophyll Synthesis, D esm arestia aculeata, Green-Light Photo­ receptor, Nitrate 
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 TEI-XML for    default:Reihe_C/46/ZNC-1991-46c-0542.pdf 
 Identifier    ZNC-1991-46c-0542 
 Volume    46 
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