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1979 (244)
81Author    J. Šonka, H. SchraudolfRequires cookie*
 Title    Incorporation of 5-Bromo-2-deoxyuridine into the Chloroplast D N A of Anemia phyllitidis  
 Abstract    5-Bromo-2-deoxyuridine, DNA, Chloroplasts, Fern 5-Bromo-2-deoxyuridine (BUdR) is incorporated into chloroplast DNA of Anemia phyllitidis. A possible connection between previously published effects of BUdR and on chloroplast ultrastructure and transitions caused by the base analogue in the DNA is referred to. 
  Reference    Z. Naturforsch. 34c, 449 (1979); received March 6 1979 
  Published    1979 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0449.pdf 
 Identifier    ZNC-1979-34c-0449 
 Volume    34 
82Author    H. Rainer, M. Aurer, Reinhard MaschlerRequires cookie*
 Title    The Influence of Spermine, Spermidine and Various Sera on T-Lymphocyte and Granulocyte Colony Growth in vitro  
 Abstract    Using T-lymphocyte (T-LC) and granulocyte colony (GC) assays with truly proliferating cells, the dose-response relationships of spermine and spermidine, of fetal calf (FCS), calf (CS), horse (HS) and human AB serum (A B S), and of the polyamines in the presence of selected does of the sera were analyzed. In contrast to earlier observations using [3H] thymidine uptake measure­ ments it was found that the polyamines as well as the sera themselves inhibit T-LC and GC growth in the presence of autologous HS and ABS, respectively. The polyamines also inhibit in the presence of additional HS and ABS, yet most effectively with added FCS and CS. Thus the previously reported species-specificity of the serum-dependent polyamine inhibition has here been shown to be more quantitative than qualitative. These studies stress the significance of assays utilizing truly dividing cells and broad dose-response relationships in order to assess specific biological effects in vitro. 
  Reference    Z. Naturforsch. 34c, 452 (1979); received January 16 1979 
  Published    1979 
  Keywords    Spermine, Spermidine, Human Serum, Lymphocyte, Granulocyte Colony Growth 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0452.pdf 
 Identifier    ZNC-1979-34c-0452 
 Volume    34 
83Author    Toshiyuki Nagata, H. »+, Ansjörg Eibl, Georg MelchersRequires cookie*
 Title    Fusion of Plant Protoplasts Induced by a Positively Charged Synthetic Phospholipid  
 Abstract    l,2-0-Dipentadecylmethylidene-glycerol-3-phosphoryl-(N-ethylamino) -ethanolamine, Dioxolane ring, ^-Potential A newly synthesized phospholipid, l,2-0-Dipentadecylmethylidene-glycerol-3-phosphoryl-(N-ethyl-amino) -ethanolamine, having positive charge was found to exercise fusion activity on plant proto­ plasts. This phospholipid is not found in cells and has an unusal chemical structure with a dioxo­ lane ring. Some properties of this compound are discussed in relation to phospholipid-induced cell fusion. 
  Reference    Z. Naturforsch. 34c, 460 (1979); received February 23 1979 
  Published    1979 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0460.pdf 
 Identifier    ZNC-1979-34c-0460 
 Volume    34 
84Author    DavidS. WilliamsRequires cookie*
 Title    The Physiological Optics of a Nocturnal Semi-Aquatic Spider, Dolomedes aquaticus (Pisauridae)  
 Abstract    The optics of the posterior eyes of the amphibious spider, Dolomedes aquaticus, are described. The lenses have strongly-curved spherical front and rear surfaces, and therefore, a fairly high light capturing ability — they have F-numbers of 0.9 — yet, the large amount of spherical aberration predicted by their shape is mostly corrected. The receptive segments of the receptors are arranged in rows, each row separated by pigmented glial cells, but within a row, rhabdomeres from neigh­ bouring cells are contiguous so that coupling between cells from the same row seems a possible way of increasing absolute sensitivity for night vision. When the spider submerges, an air-layer is held over the comeal surfaces — as with a diver's face mask — so that light is focused on to the receptive layer of the retina under water as well as above. These features are considered as adaptations for the nocturnal and semi-aquatic habits of the spider. 
  Reference    Z. Naturforsch. 34c, 463 (1979); received February 12 1979 
  Published    1979 
  Keywords    Spider, Visual Optics, Single-Lens Eye, Night Vision, Under-Water Vision 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0463.pdf 
 Identifier    ZNC-1979-34c-0463 
 Volume    34 
85Author    A. Johnsson, W. Engelmann, W. Klemke, AudTveito EkseRequires cookie*
 Title    Free-Running Human Circadian Rhythms in Svalbard  
 Abstract    The body temperature, activity-rest time, electrolytes of urine samples and mood was measured in two persons during a 19 day period under continuous light conditions in the arctic (vicinity of Ny Alesund, Svalbard-Spitsbergen). For temperature recording a new thermoprobe and a portable printer was used. Possible week Zeitgeber of the 24 hour day did not synchronize the circadian system, since circadian rhythms of about 26 hours were found. These results open up the pos­ sibility to study effects of drugs on the circadian system of humans under Svalbard conditions. In tro d u c tio n 
  Reference    Z. Naturforsch. 34c, 470 (1979); received March 13 1979 
  Published    1979 
  Keywords    Circadian Rhythms, Body Temperature, Activity, Urine Electrolytes, Humans, Svalbard-Spitsbergen 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0470.pdf 
 Identifier    ZNC-1979-34c-0470 
 Volume    34 
86Author    K. T. RindierRequires cookie*
 Title    Die Temperaturabhängigkeit biologischer Prozesse Temperature-Dependence of Biological Process  
 Abstract    Temperature dependence of biological processes is described by the modified Arrhenius equa­ tion, in which the constant is replaced by a temperature-variable coefficient. The theoretical foun­ dation for this replacement is given and experimentally verified. 1. Die Tem peraturabhängigkeit der Geschwindig­ keit chemischer Reaktionen wird durch die A rrhe­ niusgleichung beschrieben: v = v0 -e~ ElR T . 
  Reference    Z. Naturforsch. 34c, 474 (1979); eingegangen am 11. April 1978 
  Published    1979 
  Keywords    Arrhenius Equation, Phase-Change, Water, Mitochondria, Metabolizing Cell 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0474.pdf 
 Identifier    ZNC-1979-34c-0474 
 Volume    34 
87Author    José Tohá, M. Aría, Angélica Soto, M. Aría PieberRequires cookie*
 Title    On the Construction of a Phylogenetic Tree  
 Abstract    Phylogenetic Dendrogram, Molecular Evolution An algorithm for phylogenetic trees' construction is analyzed. During the past years, a great deal of inform ation on protein and nucleic acid sequence data has been obtained. These data have been very useful to throw some light on the ancestral evolution of the species. As it is well known, phylogenetic trees have been constructed considering that m utations have occurred at random and periodically. In order to determine a node in a dendrogram, the two closest species are selected. This node is now calculated as an average of the values obtained from successive comparisons of these two species with each one of the rest of the species [ 1 ]. In each comparison a system of equations of three dependent variables is solved; thereby obtaining a unique solution. For instance for Node 1 
  Reference    Z. Naturforsch. 34c, 478 (1979); received January 19 1979 
  Published    1979 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0478.pdf 
 Identifier    ZNC-1979-34c-0478 
 Volume    34 
88Author    Dieter Müller-Enoch, Helmut Thomas, Heinrich OckenfelsRequires cookie*
 Title    Eine fluorometrische Bestim m ungsm ethode für m ikrosom ale M onooxygenase-A ktivität der R attenleber m it Scoparon als Substrat A Fluorom etric Test for Microsomal Monooxygenase Activity in the Rat Liver with Scoparone as Substrate  
 Abstract    A sensitive fluorometric test for the determ ination of m onooxygenase activity in liver microsomes from rats is described. The assay is based on the O -demethylation of 6,7-dim ethoxycoum arin (scoparone) to 7-hydroxy-6-methoxy-coum arin and 6-hydroxy-7-methoxycoumarin. Pretreatm ent of rats w ith phenobarbital or polycyclic hydrocarbons (benzo[a]p yren e, 3-methylcholanthrene) causes a significant increase in the am ount of microsomal dealkylation activity. 
  Reference    Z. Naturforsch. 34c, 481 (1979); eingegangen am 27. Februar 1979 
  Published    1979 
  Keywords    Fluorom etric Test, M onooxygenases, Liver M icrosomes, Rats 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0481_n.pdf 
 Identifier    ZNC-1979-34c-0481_n 
 Volume    34 
89Author    Hartm Ut KayserRequires cookie*
 Title    Presence and B iosynthetic Im plications of /?,/?-Carotene-2-one in the M oth C erura vinula  
 Abstract    Carotenoids, /?,/?-Carotene-2one, M etabolism , In sects, Cerura /?,/?-Carotene-2-one was isolated in m inute am ounts from Cerura vinula and identified on the basis of spectra, hydride reduction, and chromatography. T he im plications on the biosynthesis of 2-hydroxylated carotenoids in in sects are discussed. 
  Reference    Z. Naturforsch. 34c, 483 (1979); received January 29 1979 
  Published    1979 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0483_n.pdf 
 Identifier    ZNC-1979-34c-0483_n 
 Volume    34 
90Author    Ivan Lacko, Ferdinand Devínsky, Ludovít Krasnec, Dušan MlynarčikRequires cookie*
 Title      
 Abstract    N,N-Dialkyl Ammonium Salts of Saturated Heterocyclic Amines, Antimicrobial Activity Antimicrobial activity of N-alkyl-N-dodecylpiperidinium bromides and N-ethyl-N-dodecylheterocycloalkyl ammonium bromides (pyrrolidine, morpholine, perhydroazepine) deter­ mined on grampositive and gramnegative bacteria, yeasts and moulds, presented as minimum inhibition concentration (MIC). Comparison of the effect of change of structure: lenghtening of alkyl chain, change of heterocyclic ring. Change in the lenght of alkyl chain markedly affects the antimicrobial activity, change of heterocyclic ring has no substantial effect. The most active compounds were N-heptyl-and N-hexyl-N-dodecylpiperidinium bromides. 
  Reference    Z. Naturforsch. 34c, 485 (1979); received February 22 1979 
  Published    1979 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0485_n.pdf 
 Identifier    ZNC-1979-34c-0485_n 
 Volume    34 
91Author    ChristopherJ Branford WhiteRequires cookie*
 Title    Some Characteristics o f Sulphate U ptake into Synaptosom es  
 Abstract    Incorporation of Na235S 0 4 by ovine brain Ficoll-prepared synaptosomes were studied as a function of time, temperature and substrate concentration. The rate of uptake obeyed Michaelis-Menten kinetics and apparent K m value of 4.4 mM Na2S04 was determined. In the presence of p-hydroxymer-curibenzoate a reduction in sulphate uptake was noted which suggests that thiol groups may possibly be implicated in the uptake mechanism. 
  Reference    Z. Naturforsch. 34c, 487 (1979); received December 22 1978/February 19 1979 
  Published    1979 
  Keywords    Synaptosomes, Sulphate Uptake, Sulphated Glycoproteins 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0487_n.pdf 
 Identifier    ZNC-1979-34c-0487_n 
 Volume    34 
92Author    Hagan BayleyRequires cookie*
 Title    Inhibitors of Photosynthetic E lectron Transport. The Properties of D iazidodialkylbenzoquinones  
 Abstract    The potential photoaffinity reagents 2,5-diazido-3-methyl-6-isopropyl-l,4-benzoquinone (DAZMIB) and 2,5-diazido-3,6-dimethyl-l,4-benzoquinone (DAZDMQ), which are analogs of the well-known inhibitor 2,5-dibromo-3-methyl-6-isopropyl-1,4-benzoquinone (DBMIB), block photosynthetic electron transport in spinach chloroplasts. On irradiation, at relatively high concentrations, DAZMlB inhibits electron flow irrevers­ ibly. However, when [3H] DAZDMQ was photolyzed with chloroplasts, no labeling of the membrane proteins was ob­ served. Rearrangements of the nitrenes derived from the oxidized or reduced azidoquinones that might account for this behaviour are discussed. 
  Reference    Z. Naturforsch. 34c, 490 (1979); received February 6 1979 
  Published    1979 
  Keywords    Photosynthetic Electron Transport, Azidoquinones, Photoaffinity Labels 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0490_n.pdf 
 Identifier    ZNC-1979-34c-0490_n 
 Volume    34 
93Author    Yngve Solberg, N.L H NorwayRequires cookie*
 Title    Studies on the Chemistry of Lichens, XIX. New Amino Compounds from Anaptychia fusca and Several Stereocaulon Species  
 Abstract    The lichen Anaptychia fusca and several Stereocaulon species have been chem ically investigat­ ed with regard to their content o f unknown ninhydrin-positive com pounds. N ew aliphatic and alicyclic, am ino com ponents isolated from water extracts o f these lichen species have been charac­ terised as aminopentitol, am inohexitol and aminocyclitetrol. To our knowledge, this is the first time that com pounds such as these have been found in lichen material. A m inopentitol and am ino­ cyclitetrol appear to be characteristic constituents o f Anaptychia fusca, whereas am inohexitol has been found in several species o f Stereocaulaceae. 
  Reference    Z. Naturforsch. 34c, 493—4 (1979); received May 15 1979 
  Published    1979 
  Keywords    Lichens, Am inopentitol, Am inohexitol, Aminocyclitetrol 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0493.pdf 
 Identifier    ZNC-1979-34c-0493 
 Volume    34 
94Author    Jürgen Jacob, Hans-Peter HanssenRequires cookie*
 Title    The Chemical Composition of Cuticular Lipids from Dragonflies (Odonata) A h rensburg/Holst, and  
  Reference    Z. Naturforsch. 34c, 498—502 (1979); received March 20 1979 
  Published    1979 
  Keywords    Cuticular Lipids, Branched Hydrocarbons, Waxes, Triglycerides, Free Fatty Acids, Insects, D ra­ gonflies, Chem otaxonom y 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0498.pdf 
 Identifier    ZNC-1979-34c-0498 
 Volume    34 
95Author    H. Sterk, H. Kopp, H. EsterbauerRequires cookie*
 Title    Messungen der Spin-Gitter-Relaxationszeit T1 an Nucleosiden Spin-Lattice-Relaxationtime 7\ M easurements o f Nucleosides  
 Abstract    Spin Lattice Relaxationtim e , N ucleosides, Nucleotides, Association Phenom ena, Temperature Dependence Nucleosides and nucleotides have been investigated by means o f the spin-lattice-relaxationtim e 7\ , the measurements carried out in CDC13 on the nucleosides adenosine (A), uridine (U) and ino­ sine (I) indicate that the strength o f H -bonding is highest in the basepair (A)-(U). The systems (A)-(A), (U) -(U) , (I)-(A) ana (I) -(U) however, show only a low tendency to associate. Plotting the spin-lattice-relaxationtime, o f the nucleotide protons in fragments (m ol weight below 40,000) originated from D N A or RNA, against increasing temperature gives a curve with a sigmoide shape and a Tm value o f 80 °C. The average o f the acüvation energy for the H -bonding is 18.02 K J/m ol. The investigations clearly show that the spin-lattice-relaxationtim e m easurem ents open first o f all a new method for research on H -bonding, secondly it is even in the case o f nucleosides and oli­ gonucleotides, where most o f the com m on m ethods fail, a way to get inform ation about H -bond-mg effects. 
  Reference    Z. Naturforsch. 34c, 503 (1979); eingegangen am 29. Januar/2. April 1979 
  Published    1979 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0503.pdf 
 Identifier    ZNC-1979-34c-0503 
 Volume    34 
96Author    H. Hofm, Ann, O. Schmut, H. Sterk, H. KoppRequires cookie*
 Title    Spin-Gitter-Relaxationszeit-Ji-Untersuchungen an Hyaluronsäure. Spin-Lattice-R elaxationtim e-r! M easurements o f Hyaluronic Acid  
 Abstract    Spin-Lattice-Relaxationtime T j , H yaluronicacid, M ethylhyaluronate, D ipol-D ipol-R elaxation-mechanism, Aggregation Reductions with [3H ]N aBH 4 proof that the decrease in viscosity o f hyaluronic acid solutions caused by lowering the pH does not depend on a depolym erisation o f hyaluronic acid. At the same time investigations at different pH -values, show a sigm oide increase o f the Spin-Lattice-R e­ laxationtime Tx. This increase depends on a progressive aggregation o f the hyaluronic acid m ole­ cule. The effect seems to be induced by the decrease o f ionization o f the carboxylgroups, by acidi­ fication o f the solution. 
  Reference    Z. Naturforsch. 34c, 508 (1979); eingegangen am 16. Februar/21. März 1979 
  Published    1979 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0508.pdf 
 Identifier    ZNC-1979-34c-0508 
 Volume    34 
97Author    Hans-Joachim BodeRequires cookie*
 Title    A Viscosity Model of Polyacrylamide Gel Electrophoresis  
 Abstract    In current theories o f polyacrylam ide gel electrophoresis, the idea prevails that molecular siev­ ing relies on different accessibility o f volum e fractions and o f cross-sectional area fractions (denot­ ed "pores") to different-sized ions due to the effect o f "geom etric exclusion". This correlates with the assum ption that all elements o f a polyacrylam ide network occupy fixed and unchangeable po­ sitions thus forcing colliding macro-ions to diffuse laterally in order to find an "accessible pore" and to resume m otion in direction o f the electrical field. H owever, the alternative conception would be equally well justified, i. e. the assum ption that polyacrylam ide chains represent smooth obstacles cleared aside under the electrokinetic pressure o f a macro-ion. This explanation would even be preferable with respect to the m olecular sieving effects occuring in solutions o f "liquid polyacrylam ide". Yet no theory exists as to describe such effects in quantitative terms. In the present article, a parameter is defined and discussed, w hich can be estim ated by experi­ ment, and which seems to be apt to characterize local resistivity o f polymer structures against dis­ location and deformation: the "fractional specific resistance" . D efinition o f this parameter is bas­ ed on the m odel o f a "viscosity-em ulsion" com posed o f two interpenetrating liquid compartments which are characterized by different levels o f hydrodynam ic friction and the spatial dimensions o f which are inferred from Ogston's theory. This concept o f "localized viscosity" m ay also serve as a link between theories o f molecular sieving and o f "m acroscopic viscosity" o f flexible polymers. The data o f Morris, formerly taken as verifications o f the "rigid-pore" concept, are now interpret­ ed in terms o f four factors responsible for sizediscrimination: collision frequency, duration o f sin­ gle contacts, size-dependent frictional force, and the extent o f cooperation am ong fibres, due to crosslinking and to simultanous contacts o f several fibres to a single macro-ion. Som e functions rel­ evant for problem s o f molecular weight determ ination by gel electrophoresis are discussed in rela­ tion to the suggested model. 
  Reference    Z. Naturforsch. 34c, 512—528 (1979); received March 22 1979 
  Published    1979 
  Keywords    Polyacrylamide, M olecular Sieve, Electrophoresis, Viscosity, Frictional Coefficient, Partition C oefficient 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0512.pdf 
 Identifier    ZNC-1979-34c-0512 
 Volume    34 
98Author    N. Jawali, J. Ram, J. K. Sainis, P. V. SaneRequires cookie*
 Title    Inhibition of the Nitrate Reductase Complex by Dibromothymoquinone  
 Abstract    Nitrate Reductase Complex, D ibrom othym oquinone, Inhibition, Electron Acceptors The plastoquinone antagonist 2,5-dibrom othym oquinone was found to inhibit N O j reduction from N A D H by the nitrate reductase com plex from wheat. It accepts electrons from N A D H through the N A D H dehydrogenase activity o f the nitrate reductase. However, it does not inhibit the reduction o f 2,6-dichlorophenol-indophenol by the enzym e. This suggests that the two com ­ pounds may be accepting electrons at different places from the enzyme. Further it was observed that reduced DCIP could be oxidized by DBM IB in the absence o f N A D H indicating that the electron flow in the nitrate reductase com plex may take place in a unidirectional way. 
  Reference    Z. Naturforsch. 34c, 529—532 (1979); received April 18 1979 
  Published    1979 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0529.pdf 
 Identifier    ZNC-1979-34c-0529 
 Volume    34 
99Author    Helm Ut, D. Uchm, Ann, Lothar TrägerRequires cookie*
 Title    Purification and Characterization of a 3,17 ß-Hydroxysteroid Dehydrogenase from Streptomyces hydrogenans  
 Abstract    Dedicated to Professor A d o lf Wacker on the Occasion o f H is 60th Birthday 3,17 ß-H ydroxysteroid Dehydrogenase, Streptom yces hydrogenans, 20 /^-Activity 3,17 ß-H ydroxysteroid dehydrogenase has been enriched and purified from cytosol o f S trepto­ myces hydrogenans. After am m onium sulfate precipitation and filtration on Sephadex G-100 the enzyme was finally purified by preparative gel electrophoresis and DEA E-Sephadex A-50 chro­ matography. P olyaciylam ide gel electrophoresis in the presence o f sodium dodecylsulfate gave a 
  Reference    Z. Naturforsch. 34c, 533—540 (1979); received March 29 1979 
  Published    1979 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0533.pdf 
 Identifier    ZNC-1979-34c-0533 
 Volume    34 
100Author    Jürgen Schallenberg, Jochen BerlinRequires cookie*
 Title    5-Methyltryptophan Resistant Cells of Catharanthus roseus  
 Abstract    Catharanthus roseus, amino acid analog resistance, indole alkaloids, tryptamine, tryptophan de­ carboxylation Several cell lines resistant to 5-methyltryptophan were selected from wild type cells of different Catharanthus roseus suspension cultures. The resistant cells had up to 30 times the normal levels of free tryptophan. Despite the increased pool size of tryptophan anthranilate synthetase activity of resistant cells was as sensitive to inhibition by L-tryptophan as wild type cells. The overproduction of tryptophan did not lead to intensified accumulation of tryptamine nor of indole alkaloids. This was supported by a low conversion of tryptophan to tryptamine in vivo and in vitro. The overpro­ duction of one of the primary precursors was evidently not sufficient to stimulate the rate of indole alkaloid synthesis in Catharanthus cells. 
  Reference    Z. Naturforsch. 34c, 541—545 (1979); received April 11 1979 
  Published    1979 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0541.pdf 
 Identifier    ZNC-1979-34c-0541 
 Volume    34 
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