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Facet   section ZfN Section C:Volume 034  [X]
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1979 (244)
41Author    A.Hlert Schmidt, Ursula ChristenRequires cookie*
 Title    A PAPS-Dependent Sulfotransferase in Cyanophora paradoxa Inhibited by 5 -AMP, 5 -ADP, and APS  
 Abstract    Cyanophora paradoxa, Sulfate Reduction, PA PS-Sulfotransferase, 3'-Phosphoadenosine-5'-phosphosulfate A 3'-phosphoadenosine-5'-phosphosulfate (PA PS) dependent sulfotransferase was partly purified from Cyanophora paradoxa. T his enzyme has a pH-optimum of 8.5 and was found to be specific for P A P S ; the K m for this nucleotide was determ ined to be 8.3 juu. In crude extracts monothiols like glutathione, m ercaptoethanol, or cysteine catalyzed a rate of about 80% of the rate obtained w ith dithioerythritol; however after purification of the PA PS-sulfotransferase the m onothiol activity decreased to about 20% of the rate obtained with dithiols. Addition of heated, crude extract partly restored the monothiol activity. This PA PS-sulfotransferase was inhibited by 5'-AD P and 5'-AM P, and especially by adenosine-5'-phosphosulfate (A PS). Practically no inhibition was found with 2'-AM P, 3'-AM P, c-AMP, 3'-5'-ADP and 2'-5'-ADP. The inhibitor constants have been determ ined to be 430 /uu for 5'-A M P; 290 /um for 5'-A D P; and 0.3 fiu for APS. These inhibitions seem to be competetive to PA PS. The role of these inhibitors for a possible regulation of assim i­ latory sulfate reduction are discussed. 
  Reference    Z. Naturforsch. 34c, 222 (1979); received January 17 1979 
  Published    1979 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0222.pdf 
 Identifier    ZNC-1979-34c-0222 
 Volume    34 
42Author    HansWerner Miiller, Margareta BaltscheffskyRequires cookie*
 Title    On the Oligomycin-Sensitivity and Subunit Composition of the ATPase Complex from Rhodospirillum rubrum  
 Abstract    Two alternative procedures for isolation of the oligomycin-sensitive ATPase complex (E. C. 3.6.1.3) from R. rubrum chromatophores are compared with respect to ease, rapidity, and yield. The inhibitory effect of oligomycin on the membrane-bound Ca2+ -ATPase activity is increased during storage of the chromatophores, whereas the effect of oligomycin on the membrane-bound Mg2+ -ATPase activity does not change within a week. Oligomycin-sensitivity of the solubilized ATPase complex depends on the isolation procedure. The enzyme complex consists of at least nine different polypeptides with the apparent molec­ ular weights of (. The polypeptides 2 — 4, 7, and 8 represent subunits of coupling factor 1. 
  Reference    Z. Naturforsch. 34c, 229 (1979); received January 4 1979 
  Published    1979 
  Keywords    Rhodospirillum rubrum, ATPase, Subunits, Oligomycin 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0229.pdf 
 Identifier    ZNC-1979-34c-0229 
 Volume    34 
43Author    B. Demmig, H. GimmlerRequires cookie*
 Title    Effect of Divalent Cations on Cation Fluxes Across the Chloroplast Envelope and on Photosynthesis of Intact Chloroplasts  
 Abstract    The effect of divalent cations on cation fluxes across the chloroplast envelope and on photo­ synthetic reactions of intact spinach chloroplasts was investigated. In the absence of EDTA, divalent cations inhibited photosynthetic C 0 2-fixation and PGA-reduction at low PGA concentrations, but had almost no effect on the reduction of OAA, BQ, and on PGA-reduction at high PGA concentrations. The inhibitory effect of Ca2+ was greater than that of M g2+. Inhibition of photosynthesis was greater when the divalent cations were added in the dark than when added in the light. In spite of its inhibitory effect, Mg2+ partially restored the Ca2+ inhibited photosynthesis, indicating the involvement of a Mg2+ /Ca2+ antagonism in the inhibitory effect. The inhibitory effect of divalent cations is stronger in a medium with low concentrations of K + than in the presence of 20 — 50 m M KC1. Mg2+ induced a release of plastidal K + and an in­ crease of stromal H + concentration. The results indicate that external Mg2+ in the absence of EDTA does not influence neither photosynthetic electron transport nor photophosphorylation, but inhibits the light activation of some enzymes of the carbon reduction cycle. The latter is assumed to be due to an acidification of the stroma pH and the decrease of endogenous K + level. Since the chloroplast envelope has only a very low permeability towards Mg2+, possible mechanisms are discussed by which M g2+ changes the properties of the chloroplast envelope and thus secondarily induces the observed effects. 
  Reference    Z. Naturforsch. 34c, 233—241 (1979); received December 28 1978 
  Published    1979 
  Keywords    Intact Chloroplasts, Chloroplast Envelope, Assimilation of C 0 2, Magnesium, Light Activation 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0233.pdf 
 Identifier    ZNC-1979-34c-0233 
 Volume    34 
44Author    Walter OettmeierRequires cookie*
 Title    A Radioactive 1,4-Benzoquinone as Inhibitor of the DBMIB-Type in Photosynthetic Electron Transport  
 Abstract    Radioactive 1.4-Benzoquinone, Photosynthetic Electron Transport, Inhibitor An 125iodine labelled 1,4-benzoquinone, an analogue of DBMIB, was studied for its interaction with the photosynthetic system of chloroplasts. It is not covalently bound to the thylakoid mem­ brane. Instead, chloroplasts slowly decompose it under release of 125iodine and formation of a quinone of only small inhibitory potency, i. e. photosynthetic activity is restored after a few minutes. The inhibition in photosynthetic electron transport caused by the 125iodine labelled 1,4-benzoquinone can also be neutralized by bovine serum albumin to which it binds covalently. 
  Reference    Z. Naturforsch. 34c, 242—249 (1979); received January 22 1979 
  Published    1979 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0242.pdf 
 Identifier    ZNC-1979-34c-0242 
 Volume    34 
45Author    Okan GurelRequires cookie*
 Title    On the Dynamic Model of tR N A : Recent Experimental Findings  
 Abstract    Recent experimental findings are shown to support various aspects of the dynamic model of tRNA proposed earlier and the interaction between tRNA, mRNA and the ribosomal RNA's. Extra loop of tRNA molecule is suggested to play a role in recognizing the corresponding amino acids and a correlation is presented between the tRNA molecules and the corresponding amino acids as tabulated by the genetic tableau. The Features of the Dynamic Model The functional role played by various parts of the tRNA molecule has been reported as: 1 . Interaction between the anticodon portion (in loop II) and the mRNA. 2 . Coupling between the 3r-end and the amino acid residue. 
  Reference    Z. Naturforsch. 34c, 248 (1979); received December 30 1978 
  Published    1979 
  Keywords    tRNA-rRNA Interactions, mRNA-16S rRNA Interactions, Genetic Tablau, tRNA Base Sequences, Extra Loop 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0248.pdf 
 Identifier    ZNC-1979-34c-0248 
 Volume    34 
46Author    Ursula Seitz, Ulrich SeitzRequires cookie*
 Title    The Molecular Weight of rRNA Precursor Molecules and Their Processing in Higher Plant Cells  
 Abstract    Actively dividing callus cells of higher plants (Petroselinum crispum, Daucus carota, Acer pseudoplatanus) were used to detect the primary gene product of rDNA in vivo. Parsley and carrot cells were labelled with [32P] orthophosphate. Under non-denaturing conditions, in both cases only one high molecular weight rRNA precursor was present on polyacrylamide gels. Its molecular weight did not exceed 2.5X10® dalton. Under denaturing conditions, 2.0 — 2.1X10® dalton were determined on formamide gels. This rRNA precursor was already present after a labelling period of 5 —10 min. In parsley cells labelled mature rRNA (25S and 18S) arrived in the cytoplasm 45 min after onset of incubation. In Acer pseudoplatanus incubated with [3H] uridine two rapidly labelled components did emerge from polyacrylamide gels without formamide; their molecular weights were 2.3 and 3.2 — 3.4X10® dalton. After electrophoresis in formamide, the larger component disappeared, thus indicating that it would be an intermolecular aggregate of different for the existence of rRNA precursors exceeding the 
  Reference    Z. Naturforsch. 34c, 253 (1979); received January 23 1979 
  Published    1979 
  Keywords    Petroselinum crispum, Daucus carota, Acer pseudoplatanus, Callus Cells, rRNA Precursor 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0253.pdf 
 Identifier    ZNC-1979-34c-0253 
 Volume    34 
47Author    MichaelS. Clegg, DieterW. GruenwedelRequires cookie*
 Title    Circular Dichroism and Ultraviolet Absorbance of Calf Thymus D NA in Presence of C H 3HgOH  
 Abstract    The changes that one observes upon the addition of CH3HgOH in the circular dichroism spec­ trum and ultraviolet absorbance spectrum of native calf thymus DNA, dissolved in buffered (pH 6.8) solutions of Na2S04 at pNa 2.0, 1.5, 1.0, and 0.0, respectively (p N a = —log [Na+ ]), are shown to be due to denaturation brought about by the organomercurial interacting with the base moieties of the polymer. The changes are characterized by an extensive shift of both spectra to longer wavelengths, by a decrease of the rotational strength of the long-wavelength positive dichroic absorption band, and by an increase in the UV absorbance at l max • Both the hyperchromicity H * of calf thymus DNA and the normalized decrease of the rotational strength of its long-wavelength positive dichromic band, Q , display cooperativity when plotted against the methylmercury concentra­ tion pM (p M = — log [CH3HgOH] a dde d) at a given salt strength. Rotational strength data, eval­ uated by integration of the area under the positive and negative dichroic absorption bands, have been tabulated for selected values of pNa and pM. They are compared with data available from the literature. In absence of CH3HgOH, and with varying salt strength, native calf thymus DNA exhibits alterations in the long-wavelength positive dichroic absorption band that are interpreted as representing B — * ■ C transitions in agreement with currently held views regarding their origin. Similar salt-induced alterations have been noted in the case of denatured DNA; their meaning in terms of DNA geometry remains unclear at this point in time. 
  Reference    Z. Naturforsch. 34c, 259—265 (1979); received November 27 1978 
  Published    1979 
  Keywords    Circular Dichroism, Ultraviolet Absorbance, Calf Thymus DNA, Methylmercury Complexes, Denaturation 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0259.pdf 
 Identifier    ZNC-1979-34c-0259 
 Volume    34 
48Author    L. Mishra, M. A. BaludaRequires cookie*
 Title    Sequences Homologous to BAV Component of HL23V in Baboon Tissues  
 Abstract    The BAV component of HL23V virus (HL23V-BAB) hybridized to DNA from different Baboon tissues and appears to be an endogenous Baboon virus. Competition hybridization studies show that HL23V-BAB is closely related to viruses isolated from various Baboon species: P. hamadrys, P. papio, and P. cynocephalus. Competition hybridization studies also show that normal Baboon tissues contain more copies of proviral DNA than cells infected in vitro. 
  Reference    Z. Naturforsch. 34c, 266 (1979); received November 2 1978 
  Published    1979 
  Keywords    HL23V-BAB, Sequence Homology, Competition Hybridization 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0266.pdf 
 Identifier    ZNC-1979-34c-0266 
 Volume    34 
49Author    Jürgen Van Der Bosch, Heinz MaierRequires cookie*
 Title    Density-Dependent Growth Inhibiting Interactions between 3T3 and SV40-3T3 Cells in Mixed Cultures  
 Abstract    3T3 cells are shown to reduce SV40-3T3 cell population growth in a density-dependent manner in co-cultures of 3T3 and SV40-3T3 cells. The development of this inhibitory activity roughly parallels the development of density-dependent inhibition of growth in homogeneous 3T3 control cultures. The extent of reduction of SV40-3T3 growth can be manipulated by pretreatment of 3T3 cells with a high serum concentration. SV40-3T3 growth rates are reduced by factors between 10 and 20 under optimum inhibitory conditions as compared to SV40-3T3 growth in control cultures. 
  Reference    Z. Naturforsch. 34c, 272 (1979); received December 19 1978 
  Published    1979 
  Keywords    3T3 Cells, SV40-3T3 Cells, Growth Inhibition, Cell Interactions 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0272.pdf 
 Identifier    ZNC-1979-34c-0272 
 Volume    34 
50Author    Jürgen Van Der Bosch, Ilse Sommer, Heinz Maier, Willy RahmigRequires cookie*
 Title    Density-Dependent Growth Adaptation Kinetics in 3T 3 Cell Populations Following Sudden [Ca2+] and Temperature Changes. A Comparison with SV40-3T3 Cells  
 Abstract    Lowered extracellular [Ca2+ ] causes low growth rates and low stationary cell densities in 3T3 cell cultures as compared to physiological [Ca2+ ]. Under otherwise constant conditions the extra­ cellular [Ca2+ ] determines a stable stationary cell density, which can be readied by increase of net cell number or decrease of net cell number, depending on cell density at the time of [Ca2+ ] adjustment. SV40-3T3 cells do not show this [Ca2+] dependency. At 39 °C 3T3 and SV40-3T3 cell populations show an increased growth rate at low cell densities as compared to cell popula­ tions at 35 °C. Approaching the stationary density the growth rate of both cell sorts is reduced faster at 39 °C than at 35 °C, leading to lower stationary cell densities at 39 °C than at 35 °C. A temperature change from 39 °C to 35 °C or in the opposite direction can affect the stationary cell density of 3T3 cell populations only if applied before reduction of growth rate by density-dependent growth-inhibiting principles has taken place. 
  Reference    Z. Naturforsch. 34c, 279 (1979); received December 19 1978 
  Published    1979 
  Keywords    Ca2+, Temperature, Growth Control, 3T3 Cell, SV40-3T3 Cell 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0279.pdf 
 Identifier    ZNC-1979-34c-0279 
 Volume    34 
51Author    W. Illi, A. RibiRequires cookie*
 Title    Structural Differences in the Tracheal Tapetum of Diurnal Butterflies  
 Abstract    Insect Eye, Diurnal Butterfly, Tracheal Tapetum lucidum The three-dimensional structure of the tracheal tapetum lucidum, its reflection properties and the resulting eye glow hue were studied in members of the diurnal butterfly families Pieridae, Nymphalidae, Satyridae and Lycaenidae. Two main groups of tapeta can be distinguished by structural and physiological differences. Whereas in pierids the main tracheal trunk at the bottom of the rhabdom bifurcates into two side branches before bifurcating again more distally, the tracheal trunk in the members of the family Nymphalidae, Satyridae and Lycaenidae investigated first divides into four side brandies. A second bifurcation shortly after the first results in eight subbranches which are regularly arranged between adjoining receptor cells. The broad banded reflection colour from incidently illuminated tapetal structures (at the level of the first bifurca­ tion) varies between and within families but does not change significantly within the same eye. Whereas in nymphalids, satyrids and lycaenids the eye glow hue corresponds with the colour of the tapetal reflection, in pierids it is dominated by the coloured receptor screening pigment. 
  Reference    Z. Naturforsch. 34c, 284 (1979); received January 15 1979 
  Published    1979 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0284.pdf 
 Identifier    ZNC-1979-34c-0284 
 Volume    34 
52Author    M. M. El-01emy, J. ReischRequires cookie*
 Title    Isolation of Batatasin I from  
 Abstract    Batatasin I was shown to occur in the fresh rhizomes of poisonous yam (Dioscorea dumetorum Pax.,). It was isolated by column chromatography and identified by chromatogra­ phic and spectral evidence. Two other unknown phenanthre-nes were shown to occur by thin-layer chromatography. 
  Reference    Z. Naturforsch. 34c, 288—289 (1979); received December 19 1978 
  Published    1979 
  Keywords    Dioscorea dumetorum, Pax, Dioscoreaceae, Poisonous Yam, Yam Batatasin I 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0288_n.pdf 
 Identifier    ZNC-1979-34c-0288_n 
 Volume    34 
53Author    JohnL. InghamRequires cookie*
 Title    Isoflavonoid Phytoalexins of P arochetus co m m u n is and F a ctorovskya aschersoniana  
 Abstract    Medicarpin has been isolated as a phytoalexin from the fungus-inoculated leaflets of Parochetus communis and Fac­ torovskya aschersoniana; a second isoflavonoid derivative, vestitol, is also produced by F. aschersoniana. Some taxono­ mic aspects of phytoalexin formation by the title species are briefly discussed. 
  Reference    Z. Naturforsch. 34c, 290—292 (1979); received December 4 1978 
  Published    1979 
  Keywords    Leguminosae, Parochetus, Factorovskya, Isoflavonoids, Phytoalexins 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0290_n.pdf 
 Identifier    ZNC-1979-34c-0290_n 
 Volume    34 
54Author    JohnL. InghamRequires cookie*
 Title    of the Genus Caragana  
 Abstract    The fungus-inoculated leaflets of 39 Caragana species have been found to variously produce five isoflavonoid phytoalexins including the known pterocarpan derivatives, medicarpin, maackiain, variabilin and pisatin. Small quan­ tities of a previously undescribed C-methylated pterocarpan were obtained from leaflets of C. acanthophylla. 
  Reference    Z. Naturforsch. 34c, 293 (1979); received December 4 1978 
  Published    1979 
  Keywords    Leguminosae, Caragana, Isoflavonoids, Pterocarpans, Phytoalexins 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0293_n.pdf 
 Identifier    ZNC-1979-34c-0293_n 
 Volume    34 
55Author    JohnL. InghamRequires cookie*
 Title    Phytoalexin Production by Flowers of Garden Pea (P isu m sa tivu m )  
 Abstract    The isoflavonoid phytoalexin pisatin has been isolated from fungus-inoculated petals of 16 Pisum sativum cultivars. Pisatin was usually accompanied by trace quantities of maackiain. No other fungitoxic compounds were detected. 
  Reference    Z. Naturforsch. 34c, 296 (1979); received December 4 1978 
  Published    1979 
  Keywords    Leguminosae, Pisum, Isoflavonoids, Pterocarpans, Phytoalexin 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0296_n.pdf 
 Identifier    ZNC-1979-34c-0296_n 
 Volume    34 
56Author    OndinaD. Colman, JuanC. StockertRequires cookie*
 Title    Electron Microscopy of Synaptonemal Complexes in Semithin Sections  
 Abstract    The technique of osmium tetroxide fixation followed by a treatment with p-phenylenediamine was applied to mouse testes in order to analyze pachytene nuclei. Observation of Epon semithin sections (0.25 — 0.5 um) in conventional elec­ tron microscopy revealed the presence of positively stained synaptonemal complexes surrounded by unstained chromatin. The present results show that by using this method a suitable contrast and resolution can be achieved for semithin sections, which facilitates the topographical study of syn­ aptonemal complexes. 
  Reference    Z. Naturforsch. 34c, 299 (1979); received December 11 1978 
  Published    1979 
  Keywords    Synaptonemal Complex, Osmium/p-Phenylenediamine, Mouse Spermatocytes, Semithin Sections, Electron Microscopy 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0299_n.pdf 
 Identifier    ZNC-1979-34c-0299_n 
 Volume    34 
57Author    Christian SteffenRequires cookie*
 Title    Absence of Microsomal Lipid Peroxidation in Acatalasemic Mice  
 Abstract    Catalase (E. C. 1.11.1.6) activity and NADPH-depen-dent lipid peroxidation have been measured in liver micro­ somes from normal and acatalasemic mice. The absence of lipid peroxidation in acatalatic microsomes is not restituted by exogenous catalase as is microsomal methanol oxidation nor is it inhibited by sodium azide, thus suggesting an additional abnormality in these mice. 
  Reference    Z. Naturforsch. 34c, 301—303 (1979); received November 27 1978 
  Published    1979 
  Keywords    Acatalasemia, Microsomes, Lipid Peroxidation, Tissue Catalase, Catalase Inhibitors 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0301_n.pdf 
 Identifier    ZNC-1979-34c-0301_n 
 Volume    34 
58Author    MichaelM. Bentley, JohnH. WilliamsonRequires cookie*
 Title    D rosophila m ela n og a ster  
 Abstract    A new locus, Aldox-2, which affects the activity and heat stability of aldehyde oxidase in D. melanogaster is described. The Aldox-2 locus is localized to map position 86 on chro­ mosome 2, between c and px. Aldehyde oxidase activity in Aldox-2 homozygotes is approximately 25 — 30% that of the Oregon-R wild-type control strain. The enzyme from the mutant stock is much more heat labile than is the enzyme from the wild-type strain. Both the activity and heat pheno­ types are completely recessive. 
  Reference    Z. Naturforsch. 34c, 304—305 (1979); received November 27 1978/January 16 1979 
  Published    1979 
  Keywords    Aldehyde Oxidase, Drosophila melanogaster, Molybdenum Hydroxylases, Aldox-2 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0304_n.pdf 
 Identifier    ZNC-1979-34c-0304_n 
 Volume    34 
59Author    W. SchäferRequires cookie*
 Title    Der Mäuse-Inzuchtstamm STU. Entwicklung und Eigenschaften The Inbred Mouse Strain STU. Development and Properties  
 Abstract    The highly inbred STU mouse strain (up to 100 inbred generations) was developed from a German white mouse colony. Its properties are described with special emphasis on the occurrence of tumors, occurrence of C-type oncorna­ viruses and on its immunological reactivity. The predominant tumor is a fibrosarcoma (~23% incidence). 
  Reference    Z. Naturforsch. 34c, 306—309 (1979); eingegangen am 7. Februar 1979 
  Published    1979 
  Keywords    Inbred Mice, Tumors, Oncorna Viruses, Immunological Reactivity 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0306_n.pdf 
 Identifier    ZNC-1979-34c-0306_n 
 Volume    34 
60Author    Horst Rimpler, Bernd SchäferRequires cookie*
 Title    Hastatosid, ein neues Iridoid aus Verbena hastata L. und Verbena officinalis L. [1] Hastatoside, a New Iridoid from Verbena hastata L. and Verbena officinalis L. [1]  
 Abstract    Verbenalin and a new iridoid, hastatoside (2) have been isolated from Verbena hastata and V. officinalis. The structure and absolute configuration of hastatoside has been determined by spectroscopical methods, and by correlating 2 chemically with verbenaline and the lactone 6. The lactone 7 has been synthesized from verbenaline. The name brasoside-tetraacetate is proposed for 7, since brasoside has been found in V. brasiliensis as a natural product. 
  Reference    Z. Naturforsch. 34c, 311 (1979); eingegangen am 14. Februar 1979 
  Published    1979 
  Keywords    Iridoid, Hastatoside, Brasoside-tetraacetat, Verbena hastata, Verbena officinalis 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0311.pdf 
 Identifier    ZNC-1979-34c-0311 
 Volume    34 
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