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1971 (381)
41Author    Jürgen KieferRequires cookie*
 Title    The Sparing Effect of UV-and X-ray-dose-fractionation in Starved Diploid Yeast  
 Abstract    The sparing effect of UV-and X-ray dose fractionation and the influence of pre-exposure star-vation were investigated in diploid yeast, Saccharomyces cerevisiae. A sparing effect could be demonstrated in starved cells if they were incubated in fresh nutrient medium during the fractio-nation interval. The time necessary for complete recovery, however, was greatly increased as com-pared to unstarved stationary phase cells. The possible role of cell progression and partial syn-chronization is discussed. Since the first experiments of JACOBSON 1 and ELKIND 2 the technique of split-dose exposure has been widely used to study recovery phenomena in irradiated cells. Generally, the effect of irradiation on colony forming ability is reduced if the total dose is given in two fractions separated by a time interval comparable to the intermitotic cycle time of the cells under study. This finding has recently been termed "the sparing effect of dose-fractiona-tion" 3 to avoid the somewhat misleading expression "recovery from sublethal damage". The effect described appears to be well established for a number of systems after exposure with ionizing 
  Reference    (Z. Naturforsch. 26b, 129—133 [1971]; eingegangen am 2. September 1970) 
  Published    1971 
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 Identifier    ZNB-1971-26b-0129 
 Volume    26 
42Author    Seymour Steven, Brody Photobiol, LabRequires cookie*
 Title    Interactions of Chlorophyll Monolayers with Electron Donors and Acceptors  
 Abstract    Photoreactions and complexation reactions between monolayers of chloroplyll a, at a water-air interface, and various electron donors and acceptor in a W il h e 1 m y film balance. When phenazine methosulfate (PMS), ascorbate, benzyl viologen (BV), or dehydroascorbic acid are present in the aqueous subphase, the area/molecule increases with substrate concentration in accordance with a Langmuir -type adsorption isotherm. The increase in area is attributed to a reorientation of chlorophyll upon complex formation. The surface potentials and areas/molecule (at zero pressure) are 350 mV and 179 Ä 2 for PMS + ascorbate in the subphase, 300 mV and 175 A 2 for PMS, 310 mV and 146 A 2 for BV, 310 mV and 156 Ä 2 for ascorbate, 230 mV and 146 Ä 2 for dehydroascorbic acid. The equilibrium constants are 170X 10 3 M~ 1 for PMS + ascor-bate, 64 x 10 3 M _1 for PMS, 41 x 10 3 M -1 for BV, and 3.9 x 10 3 M _1 for ascorbate. Formation of a complex decreases the slope of the isotherm, thereby giving the film a more liquid nature. Chloro-phyll does not complex with oxidized PMS. Illumination of the chlorophyll-BV complex in a nitrogen atmosphere results in an oxidation of chlorophyll; the photoproduct (probably Chl®-BV e) has a larger area/molecule, of 165 A 2 , and a lower surface potential, of 280 mV. Illumination of the chlorophyll • ascorbate complex or the chlorophyll • PMS • ascorbate complex results in a reduction of chlorophyll. The photoproduct of the former reaction (probably Chi®-oxidized ascorbate) has a larger area/molecule, of 169 A 2 , and a lower surface potential, of 290 mV. The photoproduct of the latter reaction (probably Chi°-PMS-oxidized ascorbate) has a larger area/molecule, of 211 A 2 , and a larger surface poten-tial, of 380 mV. No evidence is observed for a photoreaction with the Chi • PMS complex or the Chi • dehydroascorbic acid complex. A value of 0.03 is determined as a lower limit for the quantum yield of these reactions. In all cases the absorption spectrum of the chlorophyll recovered from surface after irradiation shows no significant differences from the control. In studies of photosynthetic processes electron donors (ascorbate, phenazine methosulfate) and ac-ceptors (benzyl viologen) are frequently added to maintain the photoactivity of the reaction centers 1 . To gain a better understanding of the action of these materials in vivo, their reactions are studied in a model system consisting of a monolayer of chloro-phyll (Chi). The parallels between Chi monolayers at an air-water interface and the orientation of Chi in the chloroplast have been recognized for a long time 2 ' 3 . The physical properties of Chi films are well documented and have been reviewed by KE 2 . Early reports of the action of light on Chi mono-layers were made by COLMANO 4 and BELLAMY et al. 5 . 
  Reference    (Z. Naturforsch. 26b, 134—141 [1971]; received September 21 1970) 
  Published    1971 
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 TEI-XML for    default:Reihe_B/26/ZNB-1971-26b-0134.pdf 
 Identifier    ZNB-1971-26b-0134 
 Volume    26 
43Author    E. Cabela, H. AltmannRequires cookie*
 Title    Bestimmung der Strahlenwirkung auf die Thymidinkinaseaktivität innerhalb eines Zellzyklus synchroner Chlorella Determination of Irradiation Effects on the Thymidine Kinase Activities Within a Cell-Cycle of Synchronously Growing Chlorella  
 Abstract    Ingestigations on the thymidine kinase activities within a cell-cycle of synchronously growing Chlorella cells showed a marked increase of the enzyme-activity towards the end of the DNA-reduplication. Immediately after y-irradiation with doses of 15 krad and 35 krad, no significant changes in activity could be found; three hours after irradiation a remarkable decrease of the enzyme-activity was observed. Thymidinnukleotide werden entweder durch Ein-führung einer Methylgruppe in dUMP unter Beteili-gung der Thymidylatsynthetase, oder durch Verwer-tung von Thymidin mittels Thymidinkinase gebildet. Dieses ATP-abhängige Enzym bewirkt die Phospho-rylierung von Thymidin zu Thymidin-5'-Mono-phosphat und konnte in zahlreichen Mikroorganis-men und Geweben nachgewiesen werden 1-4 . Die Bedeutung der Thymidinkinase für die DNS-Synthese ist nicht völlig geklärt. Möglicherweise spielt sie lediglich die Rolle eines Hilfsenzyms, das 
  Reference    (Z. Naturforsch. 26b, 141—143 [1971]; eingegangen am 7. August 1970) 
  Published    1971 
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 Identifier    ZNB-1971-26b-0141 
 Volume    26 
44Author    Klaus Dose, Laila ZakiRequires cookie*
 Title    Hämoproteinoide mit peroxidatischer und katalatischer Aktivität The Peroxidatic and Catalatic Activity of Hemoproteinoids  
 Abstract    Hemoproteinoids related to contemporary porphyrin-dependent peroxidases were synthesized under simple conditions. The peroxidative activity of hematin increased by a factor of 50 if the hematin was bound to proteinoids whereas the catalatic activity of hematin decreased rather under the same conditions. The peroxidative activity of hemoproteinoids particularly increased with their lysine content whereas the catalatic activity especially decreased in proteinoids with high phenyl-alanine content. The isoelectric points of the lysine-rich peroxidic hemoproteinoids were about 8. Their relatively broad pH-activity optimum was about pH 7.0. The molecular weights were a little below 20 000. Hematin content and amino acid composition of the synthetic materials were varied greatly. The substrate specificity appeared as broad as that of biogenous peroxidases, e. g., horseradish peroxidase. Among the many substrates was NADH. The possible importance of the peroxidative oxidation of NADH-type coenzymes by primitive heterotrophic organisms or pre-biological systems in an anaerobic environment is discussed. Proteinoide sind thermisch synthetisierte Poly-mere von Aminosäuren 1 . Die thermische Konden-sation wird in der Regel durch Erhitzen trockener Aminosäurengemische über den Siedepunkt des Wassers durchgeführt. Audi geochemisch plausible Kondensationsmittel wie Polyphosphate oder Di-cyandiamid 2 ' 3 sind in Simulationsexperimenten zur präbiotischen Bildung von "Urproteinen" verwandt worden. Die so synthetisierten Polymere haben in der Tat eine bemerkenswerte Ähnlichkeit mit Pro-teinen 2 . Auch schwache katalytisdie Aktivitäten konnten ihnen zugeschrieben werden. Man hat ge-funden, daß sie hydrolytische, decarboxylierende 
  Reference    (Z. Naturforsch. 26b, 144—14 [1971]; eingegangen am 2. November 1970) 
  Published    1971 
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 Identifier    ZNB-1971-26b-0144 
 Volume    26 
45Author    G. RengerRequires cookie*
 Title    II. The Acceleration of the Deactivation Reactions in the Watersplitting System by Certain Chemicals  
 Abstract    It is shown by the use of the repetitive double flash group polarography that CCCP acts as a deactivator of the active states in the water splitting system. The other electron transport reactions in system II are not influenced by CCCP. Therefore CCCP can be used both to label and to modify the properties of the water splitting system of photosynthesis. In the photosynthetic electron transport pathway electrons are transferred from H20 to NADP® ini-tiated by the photoreactive centers Chi ai and Chi an (s. Fig. 1). It was found by JOLIOT that within this electron transport chain the enzyme system Y mediating the oxidation of water is charac-» artificial e-donors X<730nm X<700nm 
  Reference    (Z. Naturforsch. 26b, 149—153 [1971]; eingegangen am 3. Oktober 1970) 
  Published    1971 
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 Identifier    ZNB-1971-26b-0149 
 Volume    26 
46Author    Ch Hintze-PodufalRequires cookie*
 Title    The Juvenile Hormone-like Effect of Farnesol Derivatives on the Pupal Development of Cerura vinula L  
 Abstract    The two synthetic farnesol derivatives, farnesyl methyl ether and the dihydrochloride of ethyl-farnesoate were found to be morphogenetically effective in a distinguishable manner in the last larval instar of Cerura vinula L. by topical application assays. The juvenile hormone-like activity of the two substances correlates with the amount of applied substances and a critical time interval to the prepupal processes. In spite of the deficiency of some prepupal processes, it is possible, that the pupal moult occurs after a long period of delay. The time lapse of the pupal development is suggested to be dependent upon some balance of the hormones of the prothoracic glands, ecdysone, and the juvenile hormone of the corpora allata. The obvious effect of the juvenile hormone during the larval development is the maintenance of larval characteristics which make possible the continuous growth of the larval form. Likewise, the behaviour of the larvae may be under direct control of the juvenile hormone. There is a close relation between behaviour and growth. For instance larvae of Galleria spin a light flat web before larval, moults but before the pupal moult they spin a tough cocoon. Implantations of active corpora allata into the last instar will cause a supernumerary lar-val moult and the spinning of a larval type of web or an intermediate type 1 . In the Sphingid Mimas tiliae there is no intermediate type of behaviour. Above a certain threshold of juvenile hormone con-centration the larvae are feeding and show the be-haviour before each larval moult, whereas below this threshold they migrate down the tree trunk to the soil for pupation 2 . The discovery that the synthetic substances farne-sol and its derivatives will reproduce the effects of the juvenile hormone, secreted by the living cor-pora allata, has provided an easy means for physio-logical studies. The purpose of the present paper is to test the effect of some farnesol derivatives upon the lapse of the pupation processes in the second part of the last larval instar of Cerura vinula L. 
  Reference    (Z. Naturforsch. 26b, 154—157 [1971]; received September 29 1970) 
  Published    1971 
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 Identifier    ZNB-1971-26b-0154 
 Volume    26 
47Author    U. Lüttge, Erika BallRequires cookie*
 Title    Light-Independent Uncoupler-Sensitive Ion Uptake by Green and by Pale Cells of Variegated Leaves of Higher Plants in Relation to Protein Content and Chloroplast Integrity  
  Reference    (Z. Naturforsch. 26b, 158—161 [1971]; eingegangen am 1. Oktober 1970) 
  Published    1971 
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 Identifier    ZNB-1971-26b-0158 
 Volume    26 
48Author    Requires cookie*
 Title    /Jk 2 k 5 (F) syn syn CH3 0 CH3  
  Reference    (Z. Naturforsch. 26b, 162 [1971]) 
  Published    1971 
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 Identifier    ZNB-1971-26b-0162_n 
 Volume    26 
49Author    E. Lindner, U. KunzeRequires cookie*
 Title    Das Verhalten von Tetraorganostannanen gegenüber wasserhaltigem Schwefeldioxid The Behaviour of Tetraorganostannanes towards aqueous Sulfurdioxide  
  Reference    (Z. Naturforsch. 26b, 164—165 [1971]; eingeg. am 16. Dezember 1970) 
  Published    1971 
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 Identifier    ZNB-1971-26b-0164_n 
 Volume    26 
50Author    A. Stuhr, H. JacobsRequires cookie*
 Title    H20  
 Abstract    umgesetzt. Die Reaktionsprodukte nimmt man mit Äther auf, filtriert (G 3) und wäscht den Rückstand mit Äther und viel Wasser. Nach dem Trocknen im Hochvakuum beträgt die Ausbeute ca. 50 Prozent. C4H10O3SSn (256,88) Ber. C 18 Man setzt 2,3 g (6,6 mMol) (ra-C4H9)4Sn mit der unter 1. angegebenen Menge S02 und H20 um. Das ölige Produkt wird durch Zugabe von Äther zur Kri-Die Krisallstruktur des Na[Yb(NH2)4] The crystal structure of Na[Yb(NH2)4] 
  Reference    (Z. Naturforsch. 26b, 165 [1971]) 
  Published    1971 
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 Identifier    ZNB-1971-26b-0165_n 
 Volume    26 
51Author    Requires cookie*
 Title    2,80  
 Abstract    2 NH, (4) 2 NHÖ (3) 2 NH, (4) 2 NHÖ (4) 2,28 2,44 2,56 2.52 (3) (3) (5) (5) Tab. 2. Yb-und Na-NH2-Abstände (in Ä). 
  Reference    (Z. Naturforsch. 26b, 166 [1971]) 
  Published    1971 
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 TEI-XML for    default:Reihe_B/26/ZNB-1971-26b-0166_n.pdf 
 Identifier    ZNB-1971-26b-0166_n 
 Volume    26 
52Author    A. S. Sideropoulos, D. M. Shankel, J. Bacterid, 96Requires cookie*
 Title    suspension  
 Abstract    After incubation at 37 °C overight, cell counts were made to calculate the percentage survivors as shown in the Figure. 120 160 240 300 Fig. 1. Dark repair of u.v.-inactivated E. coli 15T" in the presence of caffeine and cAMP. Survival of E. coli 15T in the presence of caffeine (A -A), cAMP (o — o), caffeine and cAMP (A —A) and the control (H f-). Or-dinate: Percent survival; Abscissa: UV-dose (ergs/mm 2). As shown in the Fig. 1, caffeine strongly inhibits the excision repair part of the dark reactivation in E. coli 15T~ at the u.v. doses used. This inhibition can be reversed by cAMP. It is to be noted that in the presence of cAMP alone, the dark-reactivation directs no significant influence in the survival rates. Recently it was reported that, in vivo 8 as well as in vitro 9 , caffeine depresses the excision of pyrimidine dimers, presumably by binding to the excision enzyme involved in dark repair. The reversal with cAMP of the inhibition by caffeine in our experiments indicates that both compounds compete with the phosphodiesterase-like enzymes necessary for the removal of u.v. photo-products, whereby cAMP binds to the enzyme with a greater affinity than caffeine. 
  Reference    (Z. Naturforsch. 26b, 167 [1971]) 
  Published    1971 
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 Identifier    ZNB-1971-26b-0167 
 Volume    26 
53Author    Winfried Krüger, Stephan Schnitzler, Forschungsinstitut Manfred Von Ardenne, Dresden-Weißer HirschRequires cookie*
 Title    In-vivo-Wachstumsverhalten von Zajdela- Asciteshepatomzellen nach in-vitro-Behandlung mit Trypsin und Pronase Growth of Zajdela ascites hepatoma cells in vivo following treatment with trypsin and pronase in vitro  
  Reference    (Z. Naturforsch. 26b, 169 [1971]) 
  Published    1971 
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 Identifier    ZNB-1971-26b-0169 
 Volume    26 
54Author    I. Ishiyama, A. Takatsu, G. Uhlenbruck, U. Reifenberg, St Schnitzler, 0. ProkopRequires cookie*
 Title    Helix pomatia  
 Abstract    Recently, ISHIYAMA and TAKATSU 1 suceeded in con-verting saline agglutinating anti-A from the albumin gland of the snail Helix pomatia into "incomplete", non-agglutinating anti-A by reductive cleavage and subsequent alkylation according to PORTER 2 . However it is known 3 , that this antibody-like anti-A is not only directed against the non-reducing terminal a-linked iV-Acetyl-D-galactosamine (GalNAc) of blood group A substance and other glycoproteins 4 , but also reacts with the terminal yS-anomer of GalNAc, although the affinity is much greater to the a-glycosidically bound hexos-amine 3 ' 4 . It was therefore of interest, to investigate the reac-tion of this "incomplete" anti-A with human 0 or B red blood cells (rbc), which had been treated with neuraminidase (RDE) and, as a consequence, had developed terminal /Minked GalNAc in their super-ficial glycoprotein layer, thus rendering them ag-glutinable by the normal snail agglutinin too. As these new uncovered receptors, which can be partly removed by proteolytic enzymes, are located in the outermost relief structure of the rbc membrane — whereas the blood group A specific antigens are exposed more in the deeper clefts or valleys of the surface archi-tecture — we expected, that the "incomplete" snail agglutinin, while not agglutinating A erythrocytes, would still react with RDE-treated 0 and B rbc and also agglutinate other rbc (pigeon, dude, chicken horse) known to show superficial glycoprotein bound /Minked GalNAc after enzymatic removal of neuraminic acid. In confirming our view 5 , that the question of "in-complete" agglutinins is also a membrane problem, we found (Table I), that A rbc treated with pronase were agglutinated directly in saline by the "incom-plete" anti-A, or, without treatment, were clumped by an immune serum to the heterophile snail anti-A, blocking the A receptors. In addition, A rbc "incomple-tely" coated with snail anti-A and subsequently incubated with blood group A substance (peptone) were not agglutinated by the immune serum to the snail agglu-tinin anymore, demonstrating the presence of free Reprints request to Prof. Dr. G. UHLENBRUCK, Abt. Immun-biologie der Mediz. Klinik, D-5000 Köln 41, Kerpenerstr. 15. 
  Reference    (Z. Naturforsch. 26b, 171 [1971]; received November 4 1970) 
  Published    1971 
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 Identifier    ZNB-1971-26b-0171_n 
 Volume    26 
55Author    A. M. Leseney, J. Font, R. BourrillonRequires cookie*
 Title    einiger mitogener Lektine Erythroagglutinating Properties of some mitogenic Lectins  
 Abstract    Bestimmte Agglutinine aus Pflanzen (Lektine) be-anspruchen deswegen besonderes Interesse, weil sie neben erythrozytenagglutinierenden Eigenschaften auch die Fähigkeit besitzen, Leukozyten oder Organ-und Tumorzellen erfassen zu können und außerdem noch mitogene und cytotoxische Wirkung entfalten. Diese Eigenschaften können Bestandteil eines einzigen Ag-glutininmoleküls sein, es ist aber auch durchaus mög-lich, daß sie sich auf verschiedenen (Agglutinin-) Mole-külen befinden. Deshalb haben es sich zahlreiche Ar-beitsgruppen zur Aufgabe gemacht, die Assoziation oder Dissoziation dieser biologischen Eigenschaften zu ver-folgen 1_12 . Darüber hinaus ist von Bedeutung, ob an der Zelloberfläche getrennte Rezeptoren oder ein einzi-ger Rezeptor zur Ausprägung der oben erwähnten biologischen Effekte erforderlich ist, eine Fragestellung, die ebenfalls noch bearbeitet wird 12_14 > 21 ohne daß sich bisher endgültige Schlußfolgerungen schon ziehen ließen. 
  Reference    (Z. Naturforsch. 26b, 172—173 [1971]; eingegangen am 28. Oktober 1970) 
  Published    1971 
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 Identifier    ZNB-1971-26b-0172_n 
 Volume    26 
56Author    P. L. SchellRequires cookie*
 Title    Mikroorganismen Construction of a Stopped-Flow Apparatus for Short Time Pulse-Incubation of Microorganisms and Cell Suspensions  
 Abstract    Die Aufnahme von RNS durch lebende Ehrlich-Ascites-Tumorzellen verläuft schon bei 0° derart schnell, daß mit einer normalen Inkubationstechnik keine Null-Werte erhalten werden konnten *> 2 . Deshalb wurde eine einfache Misch-Apparatur gebaut, die Inku-bationen von derartigen Zellsuspensionen bis herunter 
  Reference    (Z. Naturforsch. 26b, 174—175 [1971]; eingegangen am 1. Dezember 1970) 
  Published    1971 
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 Identifier    ZNB-1971-26b-0174_n 
 Volume    26 
57Author    U. H. EhlingRequires cookie*
 Title    Reproductive Capacity of Female Mice after Inhibition of DNA Synthesis  
 Abstract    Es mußte sichergestellt werden, daß durch hydro-dynamische Scherkräfte die Zellen nicht zerstört wur-den. Es zeigte sich, daß bei den verwendeten Materia-lien bis zu einer Einspritzzeit von ca. 0,8 sec (ca. 10 msec Inkubationszeit) eine derartige Zerstörung nicht in Erscheinung trat. Das wurde durch Zählen der Zellen in einer Neubaur -Kammer vor und nach der Passage durch die Apparatur gezeigt. Außerdem wurden unter dem Mikroskop keine Zelltrümmer ge-funden. Die Vitalität derartig behandelter Zellen konnte durch erfolgreiche Implantation sowie durch spezielle Färbung mit Eosin 6 gezeigt werden. 2. Um sicher zu sein, daß in der beschriebenen An-ordnung eine gleichmäßige, maximal gute Mischung stattfand, wurde das System in üblicher Weise entleert und die strömende Mischung durch die Durchflußküvette eines Zeiss PMQ 2-Spektrometers geleitet und die Licht-Reproduktive Leistung weiblicher Mäuse nach Blockierung der DNS-Synthese 
  Reference    (Z. Naturforsch. 26b, 175 [1971]) 
  Published    1971 
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 Identifier    ZNB-1971-26b-0175_n 
 Volume    26 
58Author    Th Günther, F. DornRequires cookie*
 Title    Die intrazelluläre Mg-Ionenaktivität in verschiedenen Säugetierzellen The intracellular Mg ion activity in different mammal cells  
  Reference    (Z. Naturforsch. 26b, 176—177 [1971]; eingegangen am 5. November 1970) 
  Published    1971 
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 Identifier    ZNB-1971-26b-0176_n 
 Volume    26 
59Author    G. ThromRequires cookie*
 Title    Elektrische Leitfähigkeit und Aktionspotential im relativen Refraktärstadium Electrical Conductance and Action Potential in the Relative Refractory Period  
  Reference    (Z. Naturforsch. 26b, 178 [1971]; eingegangen am 2. Dezember 1970) 
  Published    1971 
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 Identifier    ZNB-1971-26b-0178_n 
 Volume    26 
60Author    H.-P StockRequires cookie*
 Title    Zur Komplexbildung zwischen Molybdat-und Perjodat-Ionen in wäßrigen Lösungen Formation of Complexes of Molybdate IV with Periodate-Ions in Aqueous Solutions  
 Abstract    The existence of two presumed Mo6-and Mo7-periodate-complexes in aqueous solutions of various acidity was checked by ultracentrifugation and pH measurements. The experiments showed that only one complex exists and that the extent of protonation is but small. Die Arbeiten über die Komplexbildung zwischen Molybdationen und Per jodsäure von JAHR, FUCHS und WIESE 1 sowie weitere bisher nicht veröffent-lichte Arbeiten führten WIESE 2 zu der Annahme, daß der Komplex Na5JMo6024 der erste stabile Polymolybdat-Komplex ist, und seine Protonierung durch die Anlagerung von H2Mo04 und darauffol-gende Abspaltung von HMoO4 0 erfolgt. Er kam da-durch zu der Aufstellung des untenstehenden Sche-mas für die Reaktionsabläufe dieser Substanzen in wäßrigen Lösungen: Nr. 1 6 Na,Mo04 + 8 HCl 2 7 Na,Mo04 + 10 HCl 3 6 Na.,Mo04 -j. 9 HCl 4 7 Na2Mo04 + 11 HCl 5 6 Na2Mo04. + 10 HCl 6 7 Na,Mo04 + 12 HCl 7 6 Na"Mo04 + 11 HCl 8 7 Na2Mo04 + 13 HCl 9 6 Na2Mo04 + 12 HCl 10 7 Na2Mo04 + 14 HCl 11 6 Na.,Mo04 + 13 HCl 12 7 Na2Mo04 + 15 HCl 
  Reference    (Z. Naturforsch. 26b, 179—183 [1971]; eingegangen am 24. November 1970) 
  Published    1971 
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 Identifier    ZNB-1971-26b-0179 
 Volume    26 
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