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Facet   Publication Year 1996  [X]
Facet   section ZfN Section C:Volume 051  [X]
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1996[X]
21Author    Eugene Ucciani3, Alain Debal3, MichelG. Ruberb, R. Obert, L. WolffcRequires cookie*
 Title    Fatty Acid Composition of Some Ranunculaceae Seed Oils  
 Abstract    The fatty acid composition of seed oils of eight Ranunculaceae was determinated in order to characterize new sources of gamma-linolenic acid. Fatty acids were identified as fatty acid methyl esters (FAME) by capillary gas-liquid chromatography (GC) and capillary GC-Fourier transform infrared spectroscopy (FTIR). For trienic FAME the use of a cyanopropyl-polysiloxane stationary phase (CP-Sil 88) allowed the separation with high resolution of methyl ester of columbinic acid (trans-5,c/s-9,ds-12 18:3) and gamma-linolenic acid (cis-6,cis-9,cis-12 18:3). The results confirmed the presence of columbinic acid in Thalictrum seed oils, and that of gamma-linolenic acid in A n e m o n e and related species seed oils. The taxonomic subdivision of Ranunculaceae into sub-families and tribes, which resulted from morphological considera­ tions, did not account for the above results. 
  Reference    Z. Naturforsch. 51c, 151—154 (1996); received November 11 1995/January 5 1996 
  Published    1996 
  Keywords    Ranunculaceae, Fatty Acid Composition, Columbinic Acid, Gamma-Linolenic Acid, Taxonomy 
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 TEI-XML for    default:Reihe_C/51/ZNC-1996-51c-0151.pdf 
 Identifier    ZNC-1996-51c-0151 
 Volume    51 
22Author    Kimiaki Yamano3, Hiroshi Saitoa, Yukie Ogasawara3, Shoko Fujiia, Hiroko Yamada3, Haruhisa Shirahama3, Hiroshi KawaibRequires cookie*
 Title    The Autofluorescent Substance in the Posterior Flagellum of Swarmers of the Brown Alga Scytosiphon lomentaria  
 Abstract    S cyto sip h o n lom en taria, Brown Alga, Flagellar Autofluorescent Substance in Swarmer, Phototaxis, 4',5'-Cyclic FMN The flagellar autofluorescent substance of the brown alga S cyto sip h o n lo m en ta ria , which is probably involved in the photoreception of the phototaxis of flagellate cells, was investi­ gated. 4',5'-Cyclic FMN (1) was isolated from the extract of whole mature plants for the first time as a natural product. Since the concentration of 4',5'-cyclic FMN (1) was considerably low in vegetative plants, which do not contain fluorescent flagella, this substance is consid­ ered to correspond to the flagellar fluorescent substance. 
  Reference    Z. Naturforsch. 51c, 155—159 (1996); received October 16/January 15 1996 
  Published    1996 
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 TEI-XML for    default:Reihe_C/51/ZNC-1996-51c-0155.pdf 
 Identifier    ZNC-1996-51c-0155 
 Volume    51 
23Author    M. Auro, Sola-Pennaa, JoseR. Oberto, M. Eyer-FernandesbRequires cookie*
 Title    Trehalose Protects Yeast Pyrophosphatase against Structural and Functional Damage Induced by Guanidinium Chloride  
 Abstract    Trehalose is accumulated at very high concentrations in yeasts when this organism is sub­ mitted to a stress condition. This report approaches the question on the protective effect of trehalose and its degradation product, glucose, against structural and functional damage promoted by guanidinium on yeast cytosolic pyrophosphatase. Here it is shown that both, 1 m trehalose or 2 m glucose, are able to attenuate at almost the same extent the conforma­ tional changes promoted by guanidinium chloride on the pyrophosphatase structure. On the other hand, while 1 m trehalose increases 3.8 times the K x (from 0.15 to 0.57 m) for guanidi­ nium chloride inhibition of pyrophosphatase activity, 2 m glucose did not even duplicate this parameter (from 0.15 to 0.25 m). These data support evidences for a functional reason for the accumulation by yeasts of trehalose, and not other compound, during stress conditions. 
  Reference    Z. Naturforsch. 51c, 160—164 (1996); received September 15/November 15 1995 
  Published    1996 
  Keywords    Trehalose, Yeast, Carbohydrate, Protection, Guanidinium Chloride 
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 TEI-XML for    default:Reihe_C/51/ZNC-1996-51c-0160.pdf 
 Identifier    ZNC-1996-51c-0160 
 Volume    51 
24Author    Gabriele Scholten-BeckRequires cookie*
 Title    Magnesium Starved Cells of Euglena gracilis - a Possible Model System for Studying Mg2+ Influx?  
 Abstract    This p a p e r is d e d ic a te d to P rofessor H ans G eorg R a p p e l on the occasion o f his 60th birth day E uglena gracilis, Nutrition, Magnesium Deficiency, Magnesium Transport In order to obtain a model which allows to directly study Mg2+ influx into the cell, Mg2+ deficiency was induced in the unicellular photoautotrophic flagellate E uglena gracilis. Lack of Mg2+ in the culture medium leads to a number of morphological, biochemical, and physio­ logical changes in E uglena gracilis. The rate of cell division was reduced under Mg2+-free conditions. Subsequently an enlargement of the cells was observed and they changed from spindle-like to oval shape. The Mg2+-starved cells were well filled with paramylon granules, while their motility and vitality was not impaired. Concurrently with the larger cell size the protein-, carbohydrate-, and chlorophyll content of the cells increased. Further changes were observed in the surface carbohydrates. The proportion of cells with galactose, N-acetyl-galac-tosamine and mannose on the cell surface rose in the Mg2+-starved cultures, shown in a lectin-binding assay. Fucose was found on the pellicle of Mg2+-starved cells only. Cultivation of E uglena gracilis in Mg2+-free medium induced a drastic reduction of the intracellular Mg2+ concentration already after 24 h (from 233 nmol/106 cells to 82 nmol/106 cells). When Mg2+ was made available again, the Mg2+-starved cells took them up rapidly and the intracellular concentration of free Mg2+ rose. As Mg2+ depletion could be induced in E uglena gracilis easily by manipulating the culture conditions and as the cells remained viable, it was con­ cluded that this flagellate can be used as a model organism for studying the Mg2+ uptake of eukaryotic cells. 
  Reference    Z. Naturforsch. 51c, 165—173 (1996); received November 11/December 12 1995 
  Published    1996 
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 TEI-XML for    default:Reihe_C/51/ZNC-1996-51c-0165.pdf 
 Identifier    ZNC-1996-51c-0165 
 Volume    51 
25Author    M. Otoo Sumidaa, Shinjiro Niwata3, Takaharu Tanaka3, Tadahide Furunob, M. Amoru, Nakanishib, Ko Wakabayashic, Peter BögerdRequires cookie*
 Title    Accumulation of Protoporphyrinogen IX Induced by Acifluorfen Methyl  
 Abstract    Confocal fluorescence microscopic images were used to investigate the accumulation site of protoporphyrin IX (PPIX) within liverwort cells (Marchantia polymorpha L.) treated with peroxidizing herbicides as acifluorfen methyl (AFM). A high level of PPIX accumulation was observed in the cells during 12 h to 24 h after the addition of AFM . The results obtained from confocal fluorescence microscopic images gave clear evidence that the accumulation of PPIX occurred only in the chloroplasts, but was not observed in the cytosol or at the plasma membrane. The presence of PPIX in the chloroplasts strongly suggests that protoporphyrino­ gen (Protogen) accumulates by inhibition of protoporphyrinogen oxidase (Protox) which is the target enzyme for peroxidizing herbicides. The plastidic occurrence of PPIX provides evidence of either the presence of an additional herbicide-resistant Protox or of a non-enzy-matic Protogen-oxidation system in the Marchantia chloroplast. 
  Reference    Z. Naturforsch. 51c, 174 (1996); received D ecem ber 8/Decem ber 20 1995 
  Published    1996 
  Keywords    Protoporphyrinogen, Protoporphyrinogen Localization, Protoporphyrinogen Oxidation, Liverwort Cell Culture, Confocal Fluorescence Microscopy 
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 TEI-XML for    default:Reihe_C/51/ZNC-1996-51c-0174.pdf 
 Identifier    ZNC-1996-51c-0174 
 Volume    51 
26Author    SurendraC. Handra SabatRequires cookie*
 Title    Copper Ion Inhibition of Electron Transport Activity in Sodium Chloride Washed Photosystem II Particle Is Partially Prevented by Calcium Ion  
 Abstract    The inhibitory effects of copper ion (Cu2+) on the photosynthetic electron transport func­ tion was investigated both in NaCl washed (depleted in 17 and 23 kDa polypeptides) and native (unwashed) photosystem II membrane preparations from spinach (Beta vulgaris) chlo-roplasts. Copper in the range of 2.0 to 15 j im strongly inhibited the electron flow from water to 2,6-dichlorobenzoquinone in NaCl washed particles in a concentration dependent manner. Com plete inhibition was noticed at 15 [.im Cu2+. Oppositely in native membranes, 15 (.im C u2+ inhibited only 10-12% o f control activity. It was found that calcium ion (Ca2+) significantly reduced the Cu2+ inhibition o f electron transport activity. The Ca2+ supported prevention of Cu2+ toxicity was specific to Ca2+. Further analysis indicated that both Cu2+ and Ca2+ act competitively. Since Ca2+ is known to have stimulating/stabilizing effect at the donor side of photosystem II, it is therefore suggested that Cu2+ in NaCl washed particles exerts its inhibi­ tory effect(s) at the oxidizing side of photosystem stim ulates/stabilizes the oxygen evolution. 
  Reference    Z. Naturforsch. 51c, 179—1 (1996); received September 26 1995 
  Published    1996 
  Keywords    Calcium Ion Copper Inhibition, Electron Transport, Photosystem II Particles, Spinach (Beta vulgaris) 
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 TEI-XML for    default:Reihe_C/51/ZNC-1996-51c-0179.pdf 
 Identifier    ZNC-1996-51c-0179 
 Volume    51 
27Author    Verena Scheumann, Michael Helfrich, Siegrid Schoch, Wolfhart RüdigerRequires cookie*
 Title    Reduction of the Formyl Group of Zinc Pheophorbide b in vitro and in vivo: a Model for the Chlorophyll b to a Transformation  
 Abstract    The chemical reduction of the formyl group of pheophorbide b with sodium cyanoborohy­ dride in methanol leads to 7 '-methoxy-and 7 1-hydroxy-pheophorbide a. The same reaction with zinc pheophorbide b yields in addition zinc pheophorbide a. This was characterized by mass and 'H -NM R spectroscopy. Infiltration of zinc pheophorbides a and b and of zinc 7 1-hydroxy-pheophorbide a into etiolated oat leaves yielded phytylated products. The best yield in the esterification was obtained with 7 1-hydroxy-pheophorbide a. Analysis of the products revealed the formation of zinc pheophytin a from all infiltrated compounds. The significance for the transformation of chlorophyll b into chlorophyll a is discussed. 
  Reference    Z. Naturforsch. 51c, 185—1 (1996); received Novem ber 17/D ecem ber 18 1995 
  Published    1996 
  Keywords    Avena sativa L, Infiltration into Etiolated Leaves, Chemical Reduction, Cyanoborohydride, Phytylation 
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 TEI-XML for    default:Reihe_C/51/ZNC-1996-51c-0185.pdf 
 Identifier    ZNC-1996-51c-0185 
 Volume    51 
28Author    Petra Jekow3, Wolf-Dieter Schubert3, Petra From, M. Eb, Jochen Kruipc, ParagR. Chitnisd, Matthias Rögnerc, Wolfram Saenger3Requires cookie*
 Title    Crystallization of Intact and Subunit L-Deficient Monomers from Synechocystis PCC 6803 Photosystem I  
 Abstract    Photosystem I, PS I Monomers, Crystallization, Mesophilic Cyanobacterium Photosystem I monomers from wildtype cells o f Synechocystis PCC 6803 and from a mu­ tant deficient in the psaL gene were crystallized. PsaL encodes for the hydrophobic subunit L, which has been proposed to constitute the trimerization domain in the PS I trimer. The absence of subunit L facilitated crystallization of the PS I monomer. The unit cell dimensions and the space group for the crystals from this preparation could be determined to be a = b = 132 A , c -525 Ä, a = ß = 90°, y = 120°, the space group is Pöj or P65. The results show the potential of using specifically designed deletion mutants of an integral membrane protein for the systematic improvement o f crystal structure data. 
  Reference    Z. Naturforsch. 51c, 195—199 (1996); received October 2 4 /D ecem ber 13 1995 
  Published    1996 
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 TEI-XML for    default:Reihe_C/51/ZNC-1996-51c-0195.pdf 
 Identifier    ZNC-1996-51c-0195 
 Volume    51 
29Author    Aloysius Wild, Peter Sabel, Lucia Wild-Peters, Ursula SchmiedenRequires cookie*
 Title    Photosynthesis and Transpiration in Damaged and Undamaged Spruce Trees  
 Abstract    The investigations presented here focus on the C 0 2/H 20 gas exchange in damaged and undamaged spruce trees while using open-air measurements as well as measurements under defined conditions in the laboratory. The studies were performed at two different sites in the Hunsrück and the Westerwald mountains. In the laboratory the C 0 2/H 20 gas exchange was measured on detached branches under controlled conditions in the course of two years. C 0 2 saturation curves were also generated. In addition C 0 2 compensation points were deter­ mined employing a closed system. In the natural habitat diurnal course measurements of photosynthesis and transpiration as well as light-saturation curves for photosynthesis were performed. In parallel with the photosynthesis and transpiration measurements, measure­ ments of the water potential were taken at both locations. The photosynthetic capacity and transpiration rate show a typical annual course with pro­ nounced maxima in spring and late summer and minima in summer and winter. The needles of the damaged trees exhibit higher transpiration rates and a distinct reduction in photosyn­ thesis than the needles of the undamaged trees during two seasons. The diurnal course mea­ surements of net photosynthesis and transpiration show a maximum in photosynthesis and transpiration in the afternoon in May and September, but a characteristic midday depression in July. Photosynthesis was markedly lower and transpiration higher in the needles of the damaged trees. The damaged trees show a lower increase in the light and C 0 2 saturation curves and higher C 0 2 compensation points as compared to the undamaged trees. The water potential reaches much lower values during the course of the day in needles of the dam­ aged trees. The reduction of the photosynthetic rate on one hand and the increase in transpiration on the other hand result in an extrem e lowering of the water use efficiency in photosynthesis. The damage to the thylakoid membranes and to the guard cells obviously results in a pro­ found disturbance of the physiological hom eostasis o f the needles and could thus lead to premature needle loss. 
  Reference    Z. Naturforsch. 51c, 200 (1996); received Decem ber 27 1995/January 29 1996 D edicated to 
  Published    1996 
  Keywords    birthday C 0 2/H 20 Gas Exchange C 0 2 Compensation Point, Photosynthesis, Picea abies, Transpiration, Water Potential, Forest D isease 
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 TEI-XML for    default:Reihe_C/51/ZNC-1996-51c-0200.pdf 
 Identifier    ZNC-1996-51c-0200 
 Volume    51 
30Author    Karlheinz TempelRequires cookie*
 Title    Radioadapted Chicken Embryo Cells: Challenge Specificity and Alterations in Higher-Order D N A Structure  
 Abstract    Radioadapted chicken embryo cells (X-irradiation in ovo with 10 cGy at the 14th day of developm ent with priming periods of 24 h) were treated in vitro by challenge doses of 14 different D N A -and/or chromatin-interactive agents, including X-rays. A decrease in the cel­ lular damage, as measured by scheduled D N A synthesis, was only observed with X-irradia­ tion. Sedimentation of nucleoids as well as viscosity of alkaline lysates from ethidium bro­ mide (0 .3 5 -4 0 0 (ig/ml)-, novobiocin (1 2 5 -1 8 0 0 ^g/ml)-, and hyperthermia (30 min at 43° and 45°)-treated cells suggest a higher tendency of radioadapted cells to undergo positive D N A supercoiling. When D N A from adapted and non-adapted chicken embryo cells was used as substrate, neither its digestion by D N ase I nor the inhibition of DNase I activity by various DNA-interactive agents was changed in primed cells. From the previous investigations as well as from the present results it is concluded that an increase of tightening of protein-DNA interactions within the nuclear matrix is a molecular determinant of the elevated radiation resistance in radioadapted chicken embryo cells. 
  Reference    Z. Naturforsch. 51c, 211 (1996); received N ovem ber 29 1995/January 5 1996 
  Published    1996 
  Keywords    Chicken Embryo, Radioadaptation, Challenge Specificity, Nucleoids 
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 TEI-XML for    default:Reihe_C/51/ZNC-1996-51c-0211.pdf 
 Identifier    ZNC-1996-51c-0211 
 Volume    51 
31Author    Janina Kuczera3, Henryk Chojnackib, TeresaE. Kral3, Stanislaw Przestalski3Requires cookie*
 Title    Effect of Amphiphilic Cationic Compounds on Calcium Ion Desorption from Lecithin Liposome Membranes. Kinetic Studies and Quantum Chemical Calculations  
 Abstract    The effect o f four amphiphilic cationic compounds that differ in their polar head properties on the kinetics of calcium ion desorption from unilamellar lecithin liposom e membranes has been studied. The compounds under investigation were: N-benzyl-N,N-dimethyl-N-tetradecy-lammonium chloride (B D T A), N-methyl-N-tetradecylmorpholinium bromide (M TM), N-methyl-N-dodecyloxym ethylenem orpholinium chloride (M D O M) and N,N,N-trimethyl-N-te-tradecylammonium bromide (TM TA). Kinetic constants were determined using the three-compartmental analysis for isotopic labels. For the above m entioned compounds electronic structure calculations were performed and point charges and dipole m om ents of the molecules determined by using quantum chemis­ try methods. It was found that the rate of desorption increases with increasing concentration of the compounds studied, and effectiveness of the compounds follows the sequence: BDTA >M TM >TM TA >M DO M . For dipole moments the sequence obtained is somewhat different, namely: BDTA>M TM >M DO M >TM TA. Apparently, the differences in the effec­ tiveness of action of the compounds in the calcium desorption process follow not only from the values of dipole m om ents o f their polar heads but also from the values of point charges on som e atoms and from steric properties. 
  Reference    Z. Naturforsch. 51c, 219 (1996); received November 7 1995/January 3 1996 
  Published    1996 
  Keywords    Cationic Amphiphiles, Liposom es, Calcium Ion Desorption, Quantum Chemical Calculations 
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 TEI-XML for    default:Reihe_C/51/ZNC-1996-51c-0219.pdf 
 Identifier    ZNC-1996-51c-0219 
 Volume    51 
32Author    Janusz BlasiakRequires cookie*
 Title    Comparison of the Action of an Organophosphorus Insecticide and Its Metabolite on Chloride and Sulfate Transport in Erythrocyte Membrane  
 Abstract    A nion Transport, Erythrocyte Membrane. Organophosphorus Insecticides, M ethylparathion, Pesticides The effect of the organophosphorus insecticide methylparathion and its main metabolite methylparaoxon on chloride and sulfate equilibrium exchange in pig erythrocytes was investi­ gated using an isotope labelling technique. Efflux of both radioactive isotopes with time follow ed a single exponential. Methylparathion and methylparaoxon inhibited the chloride equilibrium exchange in erythrocyte ghosts in a dose-and time-dependent manner. There was no difference between effects evoked by these two compounds. Methylparathion and methylparaoxon inhibited sulfate efflux from resealed ghosts. The effect was also dose-and time-dependent. Again, there was no difference between the action of both agents. Dixon analysis revealed a non-com petitive character of the inhibition of the exchange of both anions with apparent K, values 183 and 184 (im for methylparathion and methylparaoxon, respec­ tively in the case o f chloride transport; for sulfate exchange these values were 675 and 648 H ,m. It was suggested that structural similarity between the parent agent and its m etabolite accounts for their identical effects. Methylparathion and methylparaoxon might inhibit the anion exchange indirectly by changing the fluidity of the erythrocyte membrane or directly by binding to the band 3 protein and evoking conformational changes that lead to the inhibi­ tion of the anion transport. The insecticides, due to their ability to phosphorylate, might also disturb som e regulation processes in the band 3 protein and affect anion transport in this way. 
  Reference    Z. Naturforsch. 51c, 226 (1996); received July 18/August 1 1995 
  Published    1996 
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 TEI-XML for    default:Reihe_C/51/ZNC-1996-51c-0226.pdf 
 Identifier    ZNC-1996-51c-0226 
 Volume    51 
33Author    Akio Kobayashi3, Mohd Ilham, Adenarr-*, Shin-Ichiro Kajiyama3, Hiroshi Kanzakic, Kazuyoshi KawazucRequires cookie*
 Title    A Cytotoxic Principle of Tamarindus indica, Di-w-butyl Malate and the Structure-Activity Relationship of Its Analogues  
 Abstract    D i-/2-butyl Malate, Tamarindus indica, Cytotoxic Compound, Sea Urchin Eggs, Structure-Activity Relationship B ioassay-gu id ed fractionation o f the m ethanolic extract o f Tamarindus indica fruits led to the isolation o f L -(-)-di-/?-b utyl m alate which exhibited a p ronou nced cytotoxic activity against sea urchin em bryo cells. In order to study structure-activity relationships, close-struc-ture relatives o f di-rc-butyl m alate w ere synthesized using d -(+) -and L -(-)-m a lic acid as starting m aterials, and their cytotoxic activities were exam in ed for the sea urchin em bryo assay. L -(-)-D i-« -p e n ty l m alate was the m ost effective inhibitor to the d ev elo p m en t o f the fertilized eggs. Significant inhibitory activity was not seen in the esters o f D -(-)-iso m e r . 
  Reference    Z. Naturforsch. 51c, 233 (1996); received November 27 1995/January 16 1996 
  Published    1996 
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 TEI-XML for    default:Reihe_C/51/ZNC-1996-51c-0233.pdf 
 Identifier    ZNC-1996-51c-0233 
 Volume    51 
34Author    M. Suwalsky3, F. Villenab, F. Aguilarc, C. P. SotomRequires cookie*
 Title    Interaction of Penicillin G with the Human Erythrocyte Membrane and Models  
 Abstract    Penicillin G, Phospholipid Bilayer, Vesicles, Erythrocyte Membrane Penicillin G (PE N) is a widely used antibiotic whose mechanism of action is related to the interference with the synthesis of bacteria cell wall. In order to evaluate its perturbing effect upon human cell membranes PEN was made to interact with human erythrocytes, isolated resealed human erythrocyte membranes and molecular models. The latter were multibilayers of the phospholipids dimyristoylphosphatidylcholine (D M PC) and dimyristoylphosphatidyl-ethanolamine (D M PE) as well as DM PC large unilamellar vesicles. These studies were per­ formed by scanning electron microscopy, fluorescence spectroscopy and X-ray diffraction methods. The observed results coincide in that PEN did not exert any significant effect upon the structures of the red cell membrane neither on its molecular models. This is in agreement with its reported lack of major toxicity and hem atological reactions. 
  Reference    Z. Naturforsch. 51c, 243 (1996); received October lO /O ctober 30 1995 
  Published    1996 
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 TEI-XML for    default:Reihe_C/51/ZNC-1996-51c-0243.pdf 
 Identifier    ZNC-1996-51c-0243 
 Volume    51 
35Author    Arno Kunze3, Ludger Witteb, M. Anuel Aregullinc, Eloy Rodriguez0, Peter Proksch3Requires cookie*
 Title    Anthraquinones in the Leaf Beetle Trirhabda geminata (Chrysomelidae)  
 Abstract    Hydroxylated anthraquinones have been found to accumulate in different developm ental stages of the chrysomelid beetle Trirhabda geminata. Eggs, larvae and adults were analyzed by HPLC and GC-MS. Each developm ental stage analyzed contained l,8-dihydroxy-3-methyl-anthraquinone (chrysophanol) and 1,8-dihydroxyanthraquinone (chrysazin). No anthraquinones were detected in the faeces of T. geminata. The level of stored anthraqui­ nones did not change during starvation. In the host plant of this specialist herbivore, the brittlebush Encelia farinosa (A steraceae), anthraquinones were not detected. Possible biolog­ ical functions of anthraquinones stored in T. geminata are discussed. 
  Reference    Z. Naturforsch. 51c, 249 (1996); received D ecem ber 4 1995/January 26 1996 
  Published    1996 
  Keywords    Trirhabda gem inata, Chrysomelidae, Anthraquinones, Chrysazin, Chrysophanol 
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 TEI-XML for    default:Reihe_C/51/ZNC-1996-51c-0249.pdf 
 Identifier    ZNC-1996-51c-0249 
 Volume    51 
36Author    M. Arkus, D. Urm, Frank-M Artin, H. Aar3, Michael Hausm, Horst Ludwigbx, ChristophC. RemRequires cookie*
 Title    Optimization of Fast-Fluorescence in situ Hybridization with Repetitive a-Satellite Probes  
 Abstract    A rapid FISH (fluorescence in situ hybridization) technique (Fast-FISH) for quantitative microscopy has been recently introduced. For highly repetitive D N A probes the hybridization (renaturation) time and the number of necessary washing steps were reduced considerably by omitting formamide or equivalent denaturing chemical agents. Due to low stringency conditions major and minor binding sites of the probes used showed visible FISH signals well suited for quantitative image-microscopy. The discrimination of minor and major binding sites was possible by automated image-processing. Here, a further, quantitative optimization of the Fast-FISH technique is described that allows to clearly discriminate major and minor binding sites of a-satellite probes by an easy image classification parameter. With respect to the optimization it was necessary to verify two sensitive parameters (hybridization time and temperature) of the given rapid FISH protocol. A s examples the systematic optimization for the two probes D 1 2 Z 2 (major binding site on the centromere of chromosome 12) and D 8 Z 2 (major binding site on the centromere of chrom osom e 8) are shown. The optimal hybridiza­ tion conditions concerning rapidness and quality o f chromosome morphology were obtained using a hybridization temperature of 70 °C and a hybridization time of 60 min. For these conditions major and minor binding sites were clearly discriminated by the intensity maxi­ mum Smax of the corresponding FISH-spots. 
  Reference    Z. Naturforsch. 51c, 253 (1996); received N ovem ber 9 1995/January 5 1996 
  Published    1996 
  Keywords    Rapid Fluorescence in situ Hybridization, Fast-FISH, Non-Formamide Protocol, Quantitative Microscopy, a-Satellite Probes 
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 TEI-XML for    default:Reihe_C/51/ZNC-1996-51c-0253.pdf 
 Identifier    ZNC-1996-51c-0253 
 Volume    51 
37Author    G. Ünter, F. Wildner3, Jürgen Schlitterh, M. Atthias, M. ÜllerbRequires cookie*
 Title    Rubisco*, an Old Challenge with New Perspectives  
  Reference    Z. Naturforsch. 51c, 263 (1996) 
  Published    1996 
  Keywords    Rubisco, Photoassimilation, Specificity, M D Simulation, Protein Engineering Invited Trends A rticle: 
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 TEI-XML for    default:Reihe_C/51/ZNC-1996-51c-0263.pdf 
 Identifier    ZNC-1996-51c-0263 
 Volume    51 
38Author    V. Bankova3, M. C. Marcuccib, S. Simova3, N. Nikolova3, A. Kujumgievc, S. Popov3Requires cookie*
 Title    Antibacterial Diterpenic Acids from Brazilian Propolis  
 Abstract    Four labdane-type diterpenic acids and syringaldehyde were isolated and identified from Brazilian propolis. A ll the com pounds exhibit antibacterial activity. The diterpenes, found for the first time in propolis, are typical for som e Araucaria species and thus indicate a possible plant source of Brazilian propolis. 
  Reference    Z. Naturforsch. 51c, 277 (1996); received Novem ber 23 1995/January 24 1996 
  Published    1996 
  Keywords    Propolis, Diterpenic Acids, Antibacterial Activity, Propolis Plant Sources 
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 TEI-XML for    default:Reihe_C/51/ZNC-1996-51c-0277.pdf 
 Identifier    ZNC-1996-51c-0277 
 Volume    51 
39Author    M. Adolphs, K. Taraz, H. BudzikiewiczRequires cookie*
 Title    Catecholate Siderophores from Chryseomonas luteola  
 Abstract    Two catecholate siderophores (chrysobactin and chryseomonin) were isolated from an iron-deficient culture medium of Chryseom onas luteola. Their structures were elucidated by chemical degradation studies and spectroscopic methods, especially 2D -N M R techniques, and confirmed by synthesis. Chryseomonin constitutes a novel type of catecholate siderophore. 
  Reference    Z. Naturforsch. 51c, 281 (1996); received January 22/February 9 1996 
  Published    1996 
  Keywords    Catecholate Siderophore, Chryseom onas luteola, Chrysobactin, Chryseomonin, Structure Elucidation 
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 TEI-XML for    default:Reihe_C/51/ZNC-1996-51c-0281.pdf 
 Identifier    ZNC-1996-51c-0281 
 Volume    51 
40Author    Maja Iv, NedjalkaV. Mitova3, E. Handjieva3-Mincho, SimeonS. Anchevb, Popov3Requires cookie*
 Title    Indoid Glucosides from Four Balkan Endemics of the Galium incurvum Group (Rubiaceae)  
 Abstract    The Balkan endemics G. mirum, Galium macedonicum, G. rhodopeum and G. aegeum, from the G. incurvum group, were screened for iridoid glucosides. Eleven known iridoid glucosides were isolated, identified and analysed for the first time in the investigated plants. The main components appeared to be asperuloside and/or the non-acetylated iridoid acids -deacetylasperulosidic acid, scandoside, monotropein. Phylogenetic relationships are discussed. 
  Reference    Z. Naturforsch. 51c, 286—2 (1996); received December 12 1995/February 12 1996 
  Published    1996 
  Keywords    Rubiaceae, Galium, Morphology, Iridoids, Phylogenetic Relationships 
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 TEI-XML for    default:Reihe_C/51/ZNC-1996-51c-0286.pdf 
 Identifier    ZNC-1996-51c-0286 
 Volume    51 
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