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1990 (204)
1Author    Joachim Schröder, D. Ru, N. SchröderRequires cookie*
 Title    Stilbene and Chalcone Synthases: Related Enzymes with Key Functions in Plant-Specific Pathways  
 Abstract    N o v em b er 7, 1989 Several years o f extensive research using the new pow erful techniques o f m olecular biology have enabled the direct co m p ariso n o f functionally o r evolutionarily related genes and their products at the nucleotide an d am in o acid sequence levels. Tw o types o f synthase w ith sim ilar functions are discussed as an interesting exam ple. Stilbene synthases, e.g. resveratrol synthase, produce the stilbene b ackbone as a key reactio n in the biosynthesis o f stilbene-type p h y to ­ alexins. C halcone synthase is a key enzym e in the biosynthesis o f flavonoids, including certain phytoalexins derived from a 6'-d eoxychalcone w hich is synthesized by coo p eratio n o f chalcone synthase w ith a reductase. R esveratrol and chalcone synthases utilize the sam e substrates (4-coum aroyl-C oA and 3 m olecules o f m alonyl-C oA) and catalyze the sam e condensing type of enzym e reaction (resulting in sequential ad d itio n o f acetate units via m alonyl-C oA), b u t the products differ in the newly form ed ring system s (resveratrol an d naringenin chalcone). A com parative analysis o f cloned D N A sequences an d o f the reaction m echanism s indicates th a t the tw o enzymes are closely related. It seems likely th a t the p roteins possess a com m on scaffold for substrate recognition and for the condensing reaction, an d th a t the different folding o f an enzym e-bound interm ediate p rio r to closure o f th e new aro m atic ring is responsible for the form ation o f the different p ro d u cts. The sam e type o f condensing reaction is utilized by the 2-ketoacyl-A C P synthases o f fatty-acid biosynthesis. H ow ever, the available d a ta indicate th a t these enzymes share little overall hom ology w ith eith er resveratrol o r chalcone synthase. One exception m ay be a short am in o acid sequence w hich co rresp o n d s to the active center o f the condensing reaction in 2-ketoacyl-A C P synthases. 
  Reference    Z. Naturforsch. 45c, 1—8 (1990); received 
  Published    1990 
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 Identifier    ZNC-1990-45c-0001 
 Volume    45 
2Author    Requires cookie*
 Title    Ascorbinsäure-Radikale als Indikator für Waldschäden - EPR-spektroskopische Untersuchungen an Fichtennadeln in vivo  
 Abstract    A scorbic Acid R adicals as Ind icato r o f D am age to F o rest -E P R Investigations o f Spruce N eedles in vivo H a rtm u t B. Stegm ann, Paul Schuler 
  Reference    Z. Naturforsch. 45c, 9 (1990) 
  Published    1990 
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 TEI-XML for    default:Reihe_C/45/ZNC-1990-45c-0009.pdf 
 Identifier    ZNC-1990-45c-0009 
 Volume    45 
3Author    Requires cookie*
 Title    Growth and Terpenoid Production of an Axenic Culture from the Liverwort Ricciocarpos natans  
 Abstract    G erald W urzel and H ans Becker Fachbereich 14.3, Pharm ak o g n osie und A nalytische Phytochem ie, U niversität des Saarlandes, D-6600 S aarbrücken 11, B undesrepublik D eutschland Z. N aturforsch. 45c, 1 3 -1 8 (1990); received S eptem ber 2 6 /O ctober 30, 1989 Ricciocarpos natans, L iverw ort, H epaticae, in vitro C u ltu re, T erpenoid Production Ricciocarpos natans grow s best on G am b o rg B5 m edium supplem ented w ith 2% sucrose. Six oth er m ineral m edia were less effective on the g row th. D uring a culture period, pH o f the m edia changed depending on the am m o n iu m a n d n itra te co nten t o f the respective m edia. In m edia o f a m olar ratio o f am m o n iu m to n itra te o f 1:1 o r 1:1.9, pH first d ro p p ed from 6.0 to ab o u t 3.5 and then rose again. In m edia w here n itra te is the only nitrogen source o r w here there is a ratio o f am m onium to n itrate o f 1:12.5, p H rose during the first two weeks and after this dropped. Differences in the light intensity (2000 an d 6000 lx) and light regime (14 h light/ 10 h dark) had no effects on the grow th. T he cu ltu res pro d u ced a variety o f terpenoids. The same p attern o f com pounds was seen in field-collected m aterial as well as in cultivated m ate­ rial. The content o f the terpenoids decreased on n itro g en and phosphorous-deficient m edia. 
  Reference    Z. Naturforsch. 45c, 13 (1990) 
  Published    1990 
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 TEI-XML for    default:Reihe_C/45/ZNC-1990-45c-0013.pdf 
 Identifier    ZNC-1990-45c-0013 
 Volume    45 
4Author    Requires cookie*
 Title    Superoxide Scavenging Properties of Flavonoids in a Non-Enzymic System  
 Abstract    A na I. H ug u et, Salvador M á ñ ez, and Marí a Jo sé A lcaraz D e p artam en t de Farm aco lo g ia i F arm acotecnia, F a cu ltat de F arm acia, A vda. Blasco Ibänez 13, 46010 V alencia, Spain Z. N a tu rfo rsch . 45c, 1 9 -2 4 (1 9 9 0); received July 17/O ctober 12, 1989 F lavonoids, S uperoxide, Free R adicals Scavengers, N itro b lu e T etrazolium T he superoxide an io n scavenging activity o f 38 flavonoids, som e o f them isolated from Side-ritis mugronensis, Sideritis javalam brensis and Cayaponia tayuya were investigated by m easu re­ m ent o f their inhibitio n o f n itro b lu e tetrazolium reduction. Isoorientin, orientin, am en to fla-vone, leucocyanidol, eriodictyol, datiscetin and robinetin behaved as p o te n t scavengers and structure-activity relationships were established. 
  Reference    Z. Naturforsch. 45c, 19 (1990) 
  Published    1990 
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 Identifier    ZNC-1990-45c-0019 
 Volume    45 
5Author    W. Olfgang SteinRequires cookie*
 Title    New Flavonoids from the Moss Bryum pseudotriquetrum  
 Abstract    ietm ar Z insm eister FB 13, B otanik, U niversität des S aarlandes, D-6600 Saarbrücken, B undesrepublik D eutschland Z. N aturforsch. 45c, 2 5 -3 1 (1990); received July 10/N ovem ber 10, 1989 Dedicated to Professor Robert Hegnauer on the occasion o f his 70th hirthdav Bryum pseudotriquetrum, B ryaceae, M usci, F lavone-O -glycosides, Flavone-C -glycosides Fifteen flavonoids have been isolated from the m oss Bryum pseudotriquetrum . The new natural products kaem pferol 3 -0 -g alacto sid e-4 '-0 -g lu co sid e, scutellarein 7-0-glucoside-6"-m alonyl ester, apigenin 7 -0 -neohesperidoside-6"-m alonyl ester and luteolin 7-O -neohesperi-doside-6"-m alonyl ester were am ongst them . F lavonols were found for the first tim e in mosses. 
  Reference    Z. Naturforsch. 45c, 25 (1990) 
  Published    1990 
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 TEI-XML for    default:Reihe_C/45/ZNC-1990-45c-0025.pdf 
 Identifier    ZNC-1990-45c-0025 
 Volume    45 
6Author    N., Jingkai Ayashi, Ding, ZhihuiD. Ing, Z. Onglian, Chen, Y. Uanfen, YiRequires cookie*
 Title    Volatile Components of the Essential Oils of Four Chinese Species in the Genus Asarum (Aristolochiaceae)  
 Abstract    , and H isashi K om ae S tudy o f E n v iro n m en tal Sciences, F aculty o f Integrated A rts an d Sciences, H iroshim a U niversity, H iroshim a 730, Jap an * K unm in g In stitu te o f B otany, T he A cadem y o f Science o f C hina, K unm ing, Y u n n an , C hina Z. N atu rfo rsch . 45c, 3 2 -3 6 (1990); received F ebruary 20/Septem ber 18, 1989 E ssential Oil, T erpene, Phenol E ther, C hem osystem atics T he volatile co m p o n en ts o f the essential oils o f four C hinese species in the genus Asarum were investigated by m eans o f G C -M S . Large am o u nts o f elem icin, safrole, an d 2-undecane to g eth er with several m ono-and sesquiterpenes were identified in the essential oils. It was fou n d th a t C hinese A sarum species is sim ilar phytochem ically to the genus H eterotropa in Jap an . 
  Reference    Z. Naturforsch. 45c, 32 (1990) 
  Published    1990 
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 Identifier    ZNC-1990-45c-0032 
 Volume    45 
7Author    G. Eo, G. G. Ross, K. Laus, D. EnzelRequires cookie*
 Title    Enzymatic Synthesis of Di-O-Phenylcarboxyl-ß-D-glucose Esters by an Acyltransferase from Oak Leaves  
  Reference    Z. Naturforsch. 45c, 37 (1990) 
  Published    1990 
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 Identifier    ZNC-1990-45c-0037 
 Volume    45 
8Author    LeszekA K Leczkow Ski, G. Erald, E. E. Dw ArdsRequires cookie*
 Title    Hysteresis and Reversible Cold Inactivation of Maize Phosphoenolpyruvate Carboxylase  
 Abstract    C4-P hotosynthesis, C old L ability, H ysteresis, P h osphoenolpyruvate C arboxylase M aize (Z e a m ays L.) leaf p h o sp h o en o lp y ru v ate (PE P) carboxylase (PE PC ase) (EC 4.1.1.31) show ed a lag in activity w hen assayed after storage at 0 -4 °C. The lag was pro m o ted by high pH on storage (7.8 -8 .5) an d was observed over a range o f assay pH (7.1 -8 .5). T herm al reacti­ vation o f the cold-stored enzym e by assay tem p eratu re (18 °C) accounted for m ost o f the hys-teretic effect, b u t presence o f P E P in the reaction m ixture was required to com pletely elim inate the lag. Based on steady-state rates after the lag, stability o f PE PC ase in the cold was inde­ pendent o f p ro tein co n cen tratio n . It is suggested th a t low tem perature and high pH induce a change in the oligom erization state o f P E PC ase, resulting in a less active b u t relatively stable form o f the enzyme. T he lag p ro b ab ly reflects a reversal o f this process, p ro m o ted by assay tem peratu re an d presence o f PEP. 
  Reference    Z. Naturforsch. 45c, 42 (1990); received July 11 1989 
  Published    1990 
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 Identifier    ZNC-1990-45c-0042 
 Volume    45 
9Author    K.Requires cookie*
 Title    The SO 4 -induced Oxidation of 2'-Deoxyuridine-5'-phosphate, Uridine-5-phosphate and Thymidine-5-phosphate. An ESR Study in Aqueous Solution  
 Abstract    n u t H ild en b ran d M ax -P lan ck -In stitu t fü r Strahlenchem ie, S tiftstraße 34, D -4330 M ülheim a. d. R u h r, B undesrepublik D eutschland Z. N atu rfo rsch . 45c, 4 7 -5 8 (1990); received A ugust 9, 1989 In m em oriam Professor Dr. O. E. P olansky Free R adicals, E SR , P yrim idine-5'-nucleotides, R adical C ation R eactions o f photolytically generated SOJ w ith 2'-deoxyuridine-5'-phosphate (5 '-d U M P), uridine-5'-phosphate (5 '-U M P) an d thym id in e-5 '-p h o sp h ate (5'-dT M P) were studied by ESR spectroscopy in aq u eo u s solution u n d er anoxic conditions. F rom 5'-d U M P and 5'-U M P the 5',5-cyclic p h o sp h a te -6-yl radicals 10 an d 11 were generated (pH 2 -1 1) w hereas from 5 '-dT M P at .pH 3 -8 the 5,6-dihydro-6-hydroxy-5-yl radical 14 and at pH 7 -1 1 the 5 -m ethylene-2'-deoxyuridine-5'-phosphate radical 15 was produced. In the experim ents w ith 5 '-U M P in ad d itio n to radical 11 the signals o f sugar radicals 12 and 13 were detected. It is assum ed th a t the base radical c atio n s act as interm ediates in the SO ^-induced radical reac­ tions. The 5 '-phosp h ate g ro u p a d d s intram olecularly to the C (5)-C (6) b ond o f the uraclilyl radical catio n w hereas the thym idyl radical cation o f 5'-dT M P reacts with H 20 at pH < 8 to yield the 6-O H -5-yl a d d u ct 14 a n d d e p ro to n ate s a t pH > 7 thus form ing the allyl-type radical 15. In 5'-U M P tran sfer o f the radical site from the base to the sugar m oiety com petes with in tram olecular p h o sp h a te a d d itio n . 
  Reference    Z. Naturforsch. 45c, 47 (1990) 
  Published    1990 
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 Identifier    ZNC-1990-45c-0047 
 Volume    45 
10Author    Agnieszka Bzowska, Ewa Kulikowska, David ShugarRequires cookie*
 Title    Properties of Purine Nucleoside Phosphorylase (PNP) of Mammalian and Bacterial Origin  
 Abstract    D ep artm en t o f B iop h ysics, In stitute o f Experim ental Physics, U niversity o f W arsaw, Zwirki i W igury 93, 02-089 W arsaw , P oland ** In stitute o f B iochem istry and B ioph ysics, P olish A cadem y o f Sciences, R ak ow ieck a 36, 02-532 W arsaw , P oland 
  Reference    Z. Naturforsch. 45c, 59 (1990) 
  Published    1990 
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 Identifier    ZNC-1990-45c-0059 
 Volume    45 
11Author    Kiriakos Kotzabasis, Horst SengerRequires cookie*
 Title    The Influence of 5-Aminolevulinic Acid on Protochlorophyllide and Protochlorophyll Accumulation in Dark-Grown Scenedesmus  
 Abstract    Fachbereich B iolo g ie/B o ta n ik , P h ilip p s-U n iversität M arburg, Lahnberge, D -3550 M arburg, B undesrepublik D eu tsch lan d 
  Reference    Z. Naturforsch. 45c, 71 (1990) 
  Published    1990 
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 Identifier    ZNC-1990-45c-0071 
 Volume    45 
12Author    Jobst-Heinrich Klemme, Gisela Laakm Ann-Ditges, Jutta MertschuweitRequires cookie*
 Title    Cellular Amino Acid Concentrations and Regulation of Aspartate Kinase in the Thermophilic Phototrophic Prokaryote Chloroflexus aurantiacus  
 Abstract    für M ik ro b io lo g ie und B iotech n ologie der U n iversität Bonn, M eckenheim er A llee 168, D -5300 B onn 1, Bundesrepublik D eutsch lan d 
  Reference    Z. Naturforsch. 45c, 74 (1990) 
  Published    1990 
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 Identifier    ZNC-1990-45c-0074 
 Volume    45 
13Author    Tibor CserháRequires cookie*
 Title    Hydrophilic Interactions between Charged Amino Acids and the Effect of Ions on the Strength of Interaction  
  Reference    Z. Naturforsch. 45c, 79 (1990) 
  Published    1990 
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 Identifier    ZNC-1990-45c-0079 
 Volume    45 
14Author    K. Kobek, H. K. LichtenthalerRequires cookie*
 Title    Effect of Different C y c l o h e x a n e -l ,3 -d i o n e Derivatives on the de novo Fatty-Acid Biosynthesis in Isolated Oat Chloroplasts  
  Reference    Z. Naturforsch. 45c, 84 (1990) 
  Published    1990 
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 Identifier    ZNC-1990-45c-0084 
 Volume    45 
15Author    Ryo Shimizu, Hajime Iwamura, Toshio FujitaRequires cookie*
 Title    Anticytokinin Activity of N-Phenyl-and N-Pyridylcarbamates  
 Abstract    D epartm ent o f A gricultural C hem istry, F acu lty o f A griculture, K y o to U n iversity, K y o to 606, Japan 
  Reference    Z. Naturforsch. 45c, 89 (1990) 
  Published    1990 
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 Identifier    ZNC-1990-45c-0089 
 Volume    45 
16Author    SamuelP. Yenne, K. Riton, K. HatziosRequires cookie*
 Title    Influence of Oxime Ether Safeners on Glutathione Content and Glutathione-Related Enzyme Activity in Seeds and Seedlings of Grain Sorghum*'*  
  Reference    Z. Naturforsch. 45c, 96 (1990) 
  Published    1990 
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 Identifier    ZNC-1990-45c-0096 
 Volume    45 
17Author    R.Alf Birkhan, Helmut KindiRequires cookie*
 Title    Re-Activation of the Expression of Glyoxysomal Genes in Green Plant Tissue  
 Abstract    B iochem ie, F ach bereich C h em ie, U n iversität M arburg, H ans-M eerw ein-Straße, D -3 5 5 0 M arburg, B undesrepublik D eu tsch lan d Z. N atu rforsch. 45c, Isocitrate L yase c D N A , M alate Syn thase c D N A , G ly o x y so m e, L eaf P eroxisom e, T ransition o f O rganelles G ly o x y so m es are being replaced by leaf-typ e peroxisom es during the greening o f dark-grow n cucum ber co ty led o n s. L ight fu n ction s in this process as negative m od u lator o f the gene expression o f glyo x y la te cycle enzym es but as p ositive regulator for the activation o f glycollate oxid ase form ation . T he differential gene exp ression w as investigated at the level o f m R N A am ou n ts using c D N A prob es hyb rid izin g w ith m alate synthase m R N A , isocitrate lyase m R N A , and g lycollate o xid ase m R N A . H yb rid ization probes were obtained from a c D N A library com p lem en tary to the germ in ation -sp ecific m R N A s o f cucum ber cotyled on s. The process o f replacem ent o f g ly o x y so m a l protein s by lea f peroxisom al proteins w as reversed to a certain extend w h en greened co ty le d o n s w ere brought back in the dark. H ybrid ization on N orthern b lots provided evid en ces that in greened c o tyled on s the am oun t o f m alate synthase m R N A and isocitrate lyase m R N A starts to increase up on dark treatm ent. 
  Reference    Z. Naturforsch. 45c, 107—111 (1990); received Septem ber 12 1989 
  Published    1990 
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 Identifier    ZNC-1990-45c-0107 
 Volume    45 
18Author    KarlG. GrellRequires cookie*
 Title    Anzeichen sexueller Fortpflanzung bei dem plasmodialen Protisten Chlorarachnion reptans Geitler* Indications of Sexual Reproduction in the Plasmodial Protist Chlorarachnion reptans Geitler  
 Abstract    The algal protists Reticulosphaera socialis Grell and Chlorarachnion reptans Geitler are "meroplasmodia": They consist o f separate cell bodies and a comm on reticulopodial network by means o f which they capture and digest food organisms, for instance diatoms. When food becomes exhausted, the meroplasmodium disintegrates sooner or later into single cells. In Chlorarachnion reptans shortage o f food may lead directly to globular resting cells with a distinct cell wall. In other cases disintegration is delayed. The plasmodial cells, still connected by reticulopodial strands, move about and join with single resting cells to form pairs. After pair formation they lose the reticulopodial connections, round o ff and form a cell wall o f their own. The cells which rested from the beginning on start growing, whereas the cells which searched for them do not. It is probable that the nuclei o f the latter are transferred into the growing cells so that synkarya are formed. If this the case, pair formation is a fertilization process and the growing cells are zygotes. A single zygote, isolated and transferred on a "lawn" o f diatoms, gives rise to a plasmodium whose cells display both sexual types again. 
  Reference    Z. Naturforsch. 45c, 112 (1990); received September 28 1989 
  Published    1990 
  Keywords    Chlorarachnion reptans Geitler, M eroplasmodium Resting Cells, Fertilization Zygotes 
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 Identifier    ZNC-1990-45c-0112 
 Volume    45 
19Author    A. Egelhaaf, K. Cölln, B. Schmitz, M. Buck, M. Wink, D. SchneiderRequires cookie*
 Title    Organ Specific Storage of Dietary Pyrrolizidine Alkaloids in the Arctiid Moth Creatonotos transiens  
 Abstract    Larvae o f the arctiid moth Creatonotos transiens obtained each 5 mg o f heliotrine, a pyrroli­ zidine alkaloid, via an artificial diet. 7 S-H eliotrine is converted into its enantiomer, 7 Ä-heliotrine, and some minor metabolites, such as callimorphine. I S -and 7 /?-heliotrine are present in the insect predominantly (more than 97%) as their N-oxides. The distribution o f heliotrine in the organs and tissues o f larvae, prepupae, pupae and imagines was analyzed by capillary gas-liquid chromatography. A large proportion o f the alkaloid is stored in the integu­ ment o f all developm ental stages, where it probably serves as a chemical defence compound against predators. Female imagines had transferred substantial amounts o f heliotrine to their ovaries and subsequently to their eggs; males partly directed it to their pheromone biosyn­ thesis. 
  Reference    Z. Naturforsch. 45c, 115 (1990); received September 25 1989 
  Published    1990 
  Keywords    Creatonotos, Arctiidae, Pyrrolizidine A lkaloids, Heliotrine M etabolism, Storage 
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 Identifier    ZNC-1990-45c-0115 
 Volume    45 
20Author    ChristophH. Artl, Sabine Fischer, Wilhelm HasselbachRequires cookie*
 Title    Closure o f a Rapidly Exchanging Calcium Compartment in Rat Cardiac Myocytes by Lanthanum  
 Abstract    Enzymatically isolated myocytes from adult rats were used to measure 45C a-uptake from salt media o f reduced Ca2+-content (0.1 m M) with normal (4 m M) or elevated (20 m M) potas­ sium concentrations. Ca2+-uptake was interrupted by filtration followed by rapid chasing the filter with salt solutions containing no Ca2+, 2 m M Ca2+ or 2 m M La3+. Rate and extent o f 45C a-uptake o f resting cells were found to be 3-fold enhanced when chas­ ing was performed with La3+-containing media. In contrast La3+ does not affect Ca2+-ex-change o f depolarized cells, the fluxes o f which approximate rates sufficiently high for contrac­ tion activation and that are sensitive to Ca2+-channel blockers. The effect o f La3+ on resting cells suggests the existence o f a small rapidly exchanging La3+-sensitive Ca2+-compartment lo­ cated adjacent to the plasma membrane. This compartment is though to be either closed or has become La3+-insensitive in activated cells. The subsarcolemmal cysternae o f the SR, the so-called "peripheral couplings" are most likely the morphological substrate o f this com part­ ment. 
  Reference    Z. Naturforsch. 45c, 121—124 (1990); received October 27 1989 
  Published    1990 
  Keywords    M yocytes, Calcium, Calcium Exchange, Lanthanum, Calcium-Channel Blockers 
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 TEI-XML for    default:Reihe_C/45/ZNC-1990-45c-0121.pdf 
 Identifier    ZNC-1990-45c-0121 
 Volume    45 
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