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1987 (238)
1Author    Z. NaturforschRequires cookie*
 Title    X-Ray Structure Analysis and Spectroscopic Data of the Antibiotic 8-(Dichloroacetyl)-5-hydroxy-2,7-dimethyl-l,4-naphthoquinone from the Fungus M ollisia sp.  
 Abstract    D edicated to P rofessor E. H ecker on the occasion o f his 60th birthday N aphthoquinone, X -R ay Structure Analysis, Mass Spectra, 13C, 'H NM R M ass spectra, X-ray data and high resolution 13C and 'H NM R spectra o f 8-(dichloroacetyl)-5-hydroxy-2,7-dim ethyl-l,4-naphthoquinone are reported. The antibiotic active com pound was isolated from the fungus M ollisia sp. 
  Reference    Z. Naturforsch. 42c, 1—3 (1987); received July 2/Septem ber 26 1986 
  Published    1987 
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 Identifier    ZNC-1987-42c-0001 
 Volume    42 
2Author    H. Einz, R. Em Bold, H. Ans, F. O. Rste, S. OnnenbichlerRequires cookie*
 Title    Structure of Azadirachtin B  
 Abstract    D edicated to Professor Erich H ecker on the occasion o f his 60th birthday N eem , Azadirachtin, Insect Growth Inhibitor, A llelochem ical, N M R , 2-D -C O SY 
  Reference    Z. Naturforsch. 42c, 4 (1987); received July 14/Decem ber 24 1986 
  Published    1987 
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 TEI-XML for    default:Reihe_C/42/ZNC-1987-42c-0004.pdf 
 Identifier    ZNC-1987-42c-0004 
 Volume    42 
3Author    Z. NaturforschRequires cookie*
 Title    /i-Alkylphenole aus Schinus terebinthifolius RADDI (Anacardiaceae)  
 Abstract    « -A lkylphenols from Schinus terebinthifolius R A D D I (A n acard iaceae) G. S kopp3, H .-J. O pferk u ch b u n d G . S chw enker3 a Pharm .-ehem . Institut der U niversität H eidelberg, Im N euenheim er Feld 364, D-6900 H eidelberg b Institut für B ioch em ie, D eutsches Krebsforschungszentrum, D edicated to P rofessor Erich H ecker on the occasion o f his 60th birthday Schinus terebinthifolius R A D D I, «-A lkylph en ols, 'H -N M R Experimental and Calculated Spec­ tra, Skin Irritation The isolation o f n-alkylphenols from the drupes o f Schinus terebinthifolius is described for the first time. The 'H -N M R param eters, especially those for the triolefinic and m onoolefinic com po­ nents, are presented. Structures were proved by calculated spectra. Skin irritating activities were determine^ 
  Reference    Z. Naturforsch. 42c, 7 (1987); received July 3 1986 
  Published    1987 
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 Identifier    ZNC-1987-42c-0007 
 Volume    42 
4Author    SalahM A D Z Ay E D, F. Athya, M. M. AhdiRequires cookie*
 Title    Methylation of Guanine in vivo by the Organophosphorus Insecticide Methamidophos  
 Abstract    D edicated to P rofessor Erich H ecker on the occasion o f his 60th birthday D N A , R N A , A lkylation, Insecticides, M ethamidophos The m ethylating capability of m etham idophos, assayed by the formation o f [7-14C]m ethyl-guanine in m ouse liver, was investigated using a 14C-insecticide labelled at the O —C H 3 group. F ollow ing i.p. administration of the toxicant, [7-14C]methylguanine could be isolated from liver nucleic acids o f treated mice. The amount o f 14C-label reached its maximum 6 h follow ing ad­ ministration o f the insecticide. At maximum l4C-labelling, the am ount o f 7-m ethylguanine calcu­ lated as fraction o f applied dose, was 20—22 x 10-4 and 98 —104 x _4, for D N A and R N A , respec­ tively. The results obtained indicate also, that an appreciable amount o f I4C-activity is incorpo­ rated via the C -l pool. 
  Reference    Z. Naturforsch. 42c, 17 (1987); received July 2/Septem ber 22 1986 
  Published    1987 
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 Identifier    ZNC-1987-42c-0017 
 Volume    42 
5Author    M. Er, M. Agdy, A. SayedRequires cookie*
 Title    Cytogenetic Effects of the Insecticide Methamidophos in Mouse Bone Marrow and Cultured Mouse Spleen Cells  
 Abstract    Insecticides, M etham idophos, Cytogenetic Effect The cytogenetic effect o f the insecticide m etham idophos (0,S-dim ethylphosphoroam idothiol-ate) was studied in m ouse bone marrow and m ouse spleen cells in culture. In vivo the ability of m etham idophos to induce micronuclei and sisterchromatid exchange in m ouse bone marrow was investigated. In vitro m ouse spleen cells in culture were used to assess the ability o f the insecticide to induce chrom osom al aberrations and sister chromatid exchange. Three different routes o f application for the pure insecticide were tested so as to cover the different possibilities for human exposure to the insecticide. Intraperitoneal, oral and dermal treatment with m etham idophos caused toxicity to marrow as indicated bv a significant increase in the percentage of polychrom atic erythrocytes (P E s) over that of the control. M etham idophos showed m utagenic potential as evidenced by a positive response in the micro­ nucleus and chrom osom al aberrations assays. Thus, single and multiple i.p. injections at 6 and 4.5 mg metham idophos/kg body w t., oral administration o f the insecticide for 14 consecutive days at a dietary level of 100 ppm and multiple dermal treatm ents (total 4) with 24 mg/kg body wt. induced a statistically significant increase in the frequency o f PEs with m icronuclei in mouse bone marrow. M oreover, the tested concentrations o f m etham idophos as low as 0.25 ng/ml induced a high percentage o f m etaphases with chrom osom al aberrations in cultured m ouse spleen cells. M etham idophos is a w eak inducer o f SCEs in m ouse bone marrow and cultured mouse spleen cells. 
  Reference    Z. Naturforsch. 42c, 21 (1987); received July 2 1986 
  Published    1987 
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 TEI-XML for    default:Reihe_C/42/ZNC-1987-42c-0021.pdf 
 Identifier    ZNC-1987-42c-0021 
 Volume    42 
6Author    H. Erm, Ann Schildknecht, Es LuboschRequires cookie*
 Title    Pflanzenabwehrstoffe XXXI [1] Molluskizide Eigenschaften von Chinonen M olluscicidal P rop erties of Q uinones  
 Abstract    Q uinones, M olluscicides, Schistosom iasis, Prim ula obconica The quinone 2-m ethoxy-6-«-pentyl-2,5-cyclohexadien-l,4-dion (Primin) was found to possess molluscicidal activity against the snail Planorbis corneus. The synthesis o f hom ologous and iso­ mers and the results in the biotests are reported. 
  Reference    Z. Naturforsch. 42c, 31 (1987); received August 6/O ctober 1 1986 
  Published    1987 
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 TEI-XML for    default:Reihe_C/42/ZNC-1987-42c-0031.pdf 
 Identifier    ZNC-1987-42c-0031 
 Volume    42 
7Author    W. Olfram, F. Ö. Rster, H. Ans, B. EckerRequires cookie*
 Title    Effects of Plant Bioregulators on the Production of Iridoid Derived Terpenoids in Valeriana wallichii and Fedia comucopiae Cell Suspension Cultures  
 Abstract    Plant Bioregulators, V alepotriates, Suspension Cultures, Valeriana wallichii, Fedia cornucopiae Four plant bioregulators were tested for their effects on production o f valepotriates in Valeriana wallichii and Fedia cornucopiae cell suspension cultures. Concentrations o f more than 10 ppm reduced valepotriate yield. A t lower concentrations production was increased. For optimal activi­ ty, bioregulators had to be applied during early exponential growth, up to day 8 of the growth cycle. A t equimolar concentrations dim ethylm orpholinium bromide (4 ppm) and dimethyl-piperidinium chloride (3 ppm) significantly im proved total valepotriates in V. wallichii (up to a n d i n F r n r n u r n n i n e (lin t o 5 0 %) 4 -r l i r h l n r n n h p n n Y v) -t r i > t h y l a m i n f ' (6 n n m) a n r l 2-(3,5-diisopropylphenoxy)-triethylam ine (6.4 ppm) increased valepotriate production in both cell cultures up to 40% . With dim ethylpiperidinium chloride and dimethylmorpholinium bromide the ratio o f m onoene to diene valepotriates in both cell systems was significantly shifted to the m onoene com pounds. A general use o f these bioregulators to increase production of terpenoid secondary m etabolites in plant tissue cultures is indicated. 
  Reference    Z. Naturforsch. 42c, 33—40 (1987); received A ugust 6 1986 D edicated to 
  Published    1987 
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 Identifier    ZNC-1987-42c-0033 
 Volume    42 
8Author    G. Eorge, F. K. Olar, M. Ichael, S. ChendzielorzRequires cookie*
 Title    Reactions of Substituted Arenediazonium Chlorides with Methylamine- Formaldehyde Premix Revisited: Reactivity and Transformations of Methylolamine Intermediates and Their Biological Significance  
 Abstract    A renediazonium Iones, M ethylolam ine Interm ediates M odulation o f the N -azo coupling betw een ring-substituted arenediazonium chlorides and prem ixed m ethylam ine-form aldehyde leads not only to l-aryl-3-hydroxym ethyl-3-m ethyltriazenes and their dim ers, but also to unexpected cyclic and com plex products. The syntheses comprise reactions with arenediazonium chlorides bearing both — M and + M substituents at para and ortho/ para positions o f the phenyl ring. O ne o f the major constituents isolated from a mixture o f products is the U -acetate ot 3-hydroxym ethyl-3-m ethyl-l-(Z,4,t>-trichlorophenyl)triazene. th is product was obtained from the reaction o f 2,4,6-trichlorobenzenediazonium chloride and m ethyl­ am ine-form aldehyde mixture which was then stabilized by acetylation. The structures o f the isolated products could be derived from reactive m ethylolam ines and electrophilic interm ediates that possibly occur in vivo and thereby offer a plausible m echanistic explanation for the carcinogenic and tumour-inhibitory activity associated with the open-chain triazene com pounds in the living cell. 
  Reference    Z. Naturforsch. 42c, 41 (1987); received O ctober 3 1986 D edicated to 
  Published    1987 
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 TEI-XML for    default:Reihe_C/42/ZNC-1987-42c-0041.pdf 
 Identifier    ZNC-1987-42c-0041 
 Volume    42 
9Author    P. E. Te, T. Ra, G. Eorg, P. Erides, SiegfriedK. Ühn, A., Em Arie, S. Ch, Erb ArthRequires cookie*
 Title    Interaction in vitro of Non-Epithelial Intermediate Filament Proteins with Histones  
 Abstract    Interm ediate Filam ent Proteins, H istones, Intermediate Filament Protein-H istone Interaction N on-epithelial interm ediate filament (IF) subunit proteins show a high and specific affinity for core histones at physiological ionic strength. W hen IF proteins are titrated with a mixture o f core histones and linker histone H I, in general the latter is totally excluded from com plexation and in the adducts form ed the moderately-arginine-rich histones H 2A and H 2B are progressively re­ placed by the very-arginine-rich histones H3 and H4. A t histone saturation, 2 m olecules o f non­ neuronal IF protein bind 1 histone H I m olecule or 8 core histone m olecules, w hereas due to its glutam ic acid rich, C terminal extensions one dimer o f thp 68 WD npnrofilament nrotein associ­ ates with 3 m olecules o f histone H I or 24 m olecules o f core histones. The salt stability o f the insoluble association products is dependent on the amount and arginine content o f the constituent histone species. Rem oval o f the non-a-helical N-and C-terminal polypeptides from IF proteins by partial chymotryptic digestion does not affect their histone-binding characteristics. Since core histones are only partially inactivated by limited tryptic digestion, they also appear to react through their a-helix-rich central domains; the limit peptide derived from histone H I is com plete­ ly inactive at physiological ionic strength. Affinity chromatography o f rod dom ains o f IF proteins on core histone-Sepharose 4B and o f histones and their limit peptides on vim entin-Sepharose 4B has shown that the interactions involving fractions o f histones H3 and H 4 are extrem ely resistant to salt and can be dissociated only with arginine or salt under denaturing conditions. In general, the experim ental results revealed close parallels betw een the association o f histones with IF proteins and their interaction with D N A . 
  Reference    Z. Naturforsch. 42c, 47 (1987); received June 18 1986 
  Published    1987 
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 Identifier    ZNC-1987-42c-0047 
 Volume    42 
10Author    Irene UrbaschRequires cookie*
 Title    Transformationen von trans-2-Hexenal durch Botrytis cinerea PERS. als Entgiftungsmechanismen Transformations of trans-2-H exenal by Botrytis cinerea PERS. as Detoxification M echanisms  
 Abstract    The m etabolization of /rö«5-2-hexenal — one of the main components of plant wound gases with antibiotic activity — was investigated for 5 different isolates of Botrytis cinerea PERS. The transform ation products as well as the kinetics of their formation were analyzed. Isolates exclusively mycelium forming (Be 1 and Be 13) transform ed rr-2-hexenal into tr-2-hexenol, while sporulating isolates (Be 3, Be 9 and Be 10) converted rr-2-hexenal to hexanol-1. Basically the m etabolization of rr-2-hexenal proceeded in the same way via the aqueous phase as in the gas phase. The transform ation products fr-2-hexenol and hexanol-1 showed significantly lower toxicity against the tested B. cinerea isolates than ?/--2-hexenal. In each isolate the end product of tr-2-hexenal conversion had the weakest inhibitory activity. The transform ation reactions thus repre­ sent detoxification mechanisms for these fungi. 
  Reference    Z. Naturforsch. 42c, 64—68 (1987); received August 26/November 12 1986 
  Published    1987 
  Keywords    Botrytis cinerea, fr-2-Hexenal Conversion, Biological Activity, Detoxification 
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 Identifier    ZNC-1987-42c-0064 
 Volume    42 
11Author    M. Ichael Wink, Ludger WitteRequires cookie*
 Title    Alkaloids in Stem Roots of Nicotiana tabacum and Spartium junceum Transformed by Agrobacterium rhizogenes  
  Reference    Z. Naturforsch. 42c, 69—72 (1987); received August 21/September 22 1986 
  Published    1987 
  Keywords    Agrobacterium rhizogenes, A lkaloids, N icotine, Cytisine, Nicotiana, Spartium 
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 Identifier    ZNC-1987-42c-0069 
 Volume    42 
12Author    Dagmar Hradetzky, EckhardW. Ollenweber, JamesN. RoitmanRequires cookie*
 Title    Flavonoids from the Leaf Resin of Snakeweed, Gutierrezia sarothrae  
 Abstract    Gutierrezia sarothrae, A steraceae, Leaf and Stem Exudate, Flavonoid Aglycones, 6,8-Dim ethoxy Flavones The resinous m aterial excreted by aerial parts of snakeweed, Gutierrezia sarothrae, was ana­ lyzed for flavonoid aglycones. Six m ajor flavonoids were characterized by their spectral data; nine m inor flavonoids were identified by direct comparisons with m arkers. The m ajor flavones form three pairs of isomeric products, five of which are 6,8-methoxylated. 
  Reference    Z. Naturforsch. 42c, 73 (1987); received August 20 1986 
  Published    1987 
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 Identifier    ZNC-1987-42c-0073 
 Volume    42 
13Author    M. Onica, E. D. Eluca, AliciaM. Seldes, EduardoG. GrosRequires cookie*
 Title    The 14ß-HydroxyIation in the Biosynthesis of Cardenolides in D igitalis purpurea. The Role of 3ß-Hydroxy-5ß-pregn-8(14)-en-20-one  
  Reference    Z. Naturforsch. 42c, 77 (1987) 
  Published    1987 
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 Identifier    ZNC-1987-42c-0077 
 Volume    42 
14Author    W. Olf-, Rainer Abraham, Burghard Stumpf, G. Bf, —. Gesellschaft, Biotechnologische Forschung, M. Bh, M. Ascheroder WegRequires cookie*
 Title    Biotransformation of Humulene by Fungi and Enantioselectivity of the Strains Used  
 Abstract    B iotransform ation, Sesquiterpene, H um ulene, Enantioselectivity, Kinetic Resolution H um ulene was transform ed by strains of Chaetomium cochlioides and Diplodia gossypina ATCC 10936 affording 33 new hum ulanes. The first step is a non-enantioselective epoxidation of the 1,2-double bond, whereas the subsequent hydroxylations were perform ed preferably at the lR ,2R -epoxide. To proof these results the anti-1,2;8,9-diepoxide was synthesized and used as substrate. While the lR ,2R ,8S,9S-antipode of this diepoxide was hydroxylated the other one was recovered unchanged. Diplodia gossypina A TCC 10936 gave the same products but differing in relative and absolute configurations. 
  Reference    Z. Naturforsch. 42c, 79—86 (1987); received Septem ber 2 1986 
  Published    1987 
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 Identifier    ZNC-1987-42c-0079 
 Volume    42 
15Author    StephanW. Schmidt, KlausD. Enzel, Gerhard Schilling, GeorgG. GrossRequires cookie*
 Title    Enzymatic Synthesis of 1,6-Digalloylglucose from ß-Glucogallin by ß-Glucogallin: ß-Glucogallin 6-0-Galloyltransferase from Oak Leaves  
 Abstract    ß-Glucogallin (1 -O-galloyl-ß-D-glucopyranose), 1,6-Di-O-galloyl-ß-D-glucopyranose, Acyltransfer-ase, G allotannin Biosynthesis, Quercus robur Cell-free extracts from oak (Quercus robur) leaves catalyze the transfer of the galloyl-moiety of ß-glucogallin (1-O-galloyl-ß-D-glucopyranose) specifically to the 6-position of the same com pound, yielding 1,6-di-O-galloyl-ß-D-glucopyranose, an interm ediate of gallotannin biosynthesis, ß-Glucogallin thus functions as both donor and acceptor molecule in this reaction. The partial purification and some general properties of this new acyltransferase are reported. 
  Reference    Z. Naturforsch. 42c, 87 (1987); received July 27 1986 
  Published    1987 
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 Identifier    ZNC-1987-42c-0087 
 Volume    42 
16Author    JensD. Schwenn, Ulrich SchriekRequires cookie*
 Title    PAPS-Reductase from Escherichia coli: Characterization of the Enzyme as Probe for Thioredoxins  
 Abstract    29/O ctober 8, 1986 Thioredoxins, Specificity as C osubstrate, PA PS-R eductase, Cross-Reactivity, Sulphate R educ­ tion PA PS-reductase from Escherichia coli was em ployed to detect thioredoxins from pro-and eukaryotic organisms. A simple m ethod for the isolation of this enzyme and properties of the enzymatic assay were described. A comparison betw een thioredoxins detected by the PAPS-reductase and the Fructose-bisphosphatase or N A D P m alate dehydrogenase was used to assess the validity of the test. The high cross-reactivity of the bacterial enzyme was useful in the purifica­ tion of heterologous thioredoxins from spinach, Synechococcus, and Saccharomyces cerevisiae. 
  Reference    Z. Naturforsch. 42c, 93 (1987); received July 
  Published    1987 
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 Identifier    ZNC-1987-42c-0093 
 Volume    42 
17Author    Christine Schindler, Ruth Hracky, Jürgen SollRequires cookie*
 Title    Protein Transport in Chloroplasts: ATP is Prerequisit  
 Abstract    The energy requirem ent for protein transport into chloroplast was assayed under conditions that perm it to distinguish whether the posttranslational translocation is dependent on A TP or w hether a m em brane potential across the chloroplast envelope can drive this transport event. A m em brane potential is not required for translocation. A TP can support protein transport in the presence of protonophores and ionophores. Non-hydrolyzable A TP analogues and G TP, CTP, U T P cannot serve as ATP substitutes. Translocation could be observed when an A TP generating system was used to supply ATP. In contrast ATP degrading systems completely abolished translocation. The inner envelope mem brane localized ATP-ase is probably not involved in the transport event. The results suggest that ATP is needed at the outer chloroplast envelope. Inhibition of protein transport by A D P , pyrophosphate and NaF is studied and its con­ sequences discussed. 
  Reference    Z. Naturforsch. 42c, 103 (1987); received August 26 1986 
  Published    1987 
  Keywords    Protein T ransport, ATP-Hydrolysis, Chloroplasts, Envelope, Pisum sativum 
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 Identifier    ZNC-1987-42c-0103 
 Volume    42 
18Author    Joachim Peschke, Helmuth MöhwaldRequires cookie*
 Title    Lipid Influence on the Structure of the Light Harvesting B 800—850 Proteins  
 Abstract    Lipid/Protein Interaction, A ntenna, Photosynthesis, M em brane Structure Interaction of the antenna protein B 800—850 with the lipid environm ent and with the reaction center of the photosynthetic bacterium Rhodopseudomonas sphaeroides is studied by fluorescence spectroscopy, transient absorption techniques, light scattering and electron microscopy. Using vesicles of synthetic phospholipids it is shown that solidification of the m em brane causes a struc­ tural protein change evident from a reduction in fluorescence quantum yield. The change occurs at a tem perature up to 5 °C below that corresponding to the gel/fluid transition tem perature and indicates local melting. The structural change is not specific for the lipid head group nor chain length (investigated for lengths of 12 to 16 CH2 groups) and can be understood applying a simple e i a s u c m u d e i. I i c a n a is u u c in d u c e d ls u ilic iiiia lly b y c h a n g i n g ilic lu in e m ilie u a n d Luus v a iy n ig the lipid phase state. Energy transfer LH C P—»RC is proven to be highly efficient in model m em branes and is not affected by the existence of a phase transition. This indicates two LH CP fractions one tightly -and one non-bound to the RC. 
  Reference    Z. Naturforsch. 42c, 109 (1987); received Septem ber 4 1986 
  Published    1987 
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 Identifier    ZNC-1987-42c-0109 
 Volume    42 
19Author    PaulH. Ansm AnnRequires cookie*
 Title    Daffodil Chromoplast DNA: Comparison with Chloroplast D NA, Physi­ cal Map, and Gene Localization  
 Abstract    In daffodil, developm ent of chloroplasts or chromoplasts is not accompanied by plastid D NA modification. This has been shown by comparing the fragment pattern of different restriction endonucleases, and by the lack of m ethylation of CCGG sequences. A physical map has been constructed for the plastom e using the restriction endonucleases Sal I, Pst I, and Bgl I. The fragments containing the genes for the large subunit of ribulose bisphosphate carboxylase/oxygen­ ase (rbcL), the alpha, beta, and epsilon subunits of the A TP synthase complex (atpA , atpB , atpE), c y to c h ro m e /(p e tA), and for the 51 kDa chlorophyll apoprotein of photosystem II (psbB) have been identified. The respective gene locations correspond to those on spinach chloroplast DNA. 
  Reference    Z. Naturforsch. 42c, 118 (1987); received August 14 1986 
  Published    1987 
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 Identifier    ZNC-1987-42c-0118 
 Volume    42 
20Author    ThorB. MeløRequires cookie*
 Title    Uptake of Protoporphyrin and Violet Light Photodestruction of Propionibacterium acnes  
 Abstract    The uptake of protoporphyrin IX by Propionibacterium acnes in suspension has been studied by fluorescence spectroscopy. Protoporphyrin, after it was injected into a cell suspension, was firstly bound to receptors on the cell surface and in this state protoporphyrin was non-fluorescent. Subsequently, probably as a result of lateral diffusion in the cell wall, these protoporphyrin-receptor complexes formed dimers. The final step in the overall uptake process of protoporphyrin by the cells from the surroundings consisted in a jum p of such dim ers from w aterlike to lipidlike com partm ents in the cell m em brane w here protoporphyrin becam e fluorescent. The lipidlike com partm ents in the cells had a limited binding capacity of protoporphyrin. The fiaciiuu ui suiviving ceils veisus iigin uuse has aiso been studied for varying am ounts of protoporphyrin added to the cell suspensions. The survival curves were exponentially decaying with the irradiation time and there was a direct proportionality betw een the inverse slope of the survival curves and the intensity of protoporphyrin fluorescence from the lipidlike com partm ents. The relevance of these results to the therapy of Acne vulgaris is also discussed. 
  Reference    Z. Naturforsch. 42c, 123 (1987); received June 18/September 15 1986 
  Published    1987 
  Keywords    Propionibacterium acnes, Protoporphyrin, Photoinactivation Photosensitization, Protoporphyrin U ptake 
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 TEI-XML for    default:Reihe_C/42/ZNC-1987-42c-0123.pdf 
 Identifier    ZNC-1987-42c-0123 
 Volume    42 
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