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1983 (194)
1Author    W. EstgasseRequires cookie*
 Title    Eine Röntgenkleinwinkelkamera ohne Spaltlängenverschmierung, II  
 Abstract    A more detailed description o f the small-angle-X-ray camera without slithigh-error is given together with a calculation o f the beam condition and its influence on different scattering functions. Also the proceeding to measure molecular weights is described and som e scattering curves are figured as an exam ple o f the equipments efficiency. 
  Reference    Z. Naturforsch. 38c, 1—6 (1983); received June 11/A ugust 16 1982 
  Published    1983 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0001.pdf 
 Identifier    ZNC-1983-38c-0001 
 Volume    38 
2Author    SiegmUnd Lang, H.Anna Beyer, P. Eter, Sistig, F. Ritz, W. Ag, N. ErRequires cookie*
 Title    A New Red Dipyrromethene Pigment from Candida boidinii  
 Abstract    The yeast Candida boidinii ATCC 32195 produces a cell-bound red pigment during growth on 1% ethanol in the presence o f 0.05% monofluoracetate. Its isolation and purification are described. The structure was elucidated by chemical degradation and physical m ethods, m ainly mass and nuclear magnetic resonance spectroscopy. 
  Reference    Z. Naturforsch. 38c, 7 (1983); received September 15/O ctober 28 1982 
  Published    1983 
  Keywords    Candida boidinii, Growth on Ethanol, Presence M onofluoracetate, Red Dipyrrom ethene Pigment, Structure Elucidation 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0007.pdf 
 Identifier    ZNC-1983-38c-0007 
 Volume    38 
3Author    Z.Requires cookie*
 Title    Ellagitannine aus den Blättern von Vitis vinifera  
 Abstract    E lla g ita n n in s from th e L e a v e s o f Vitis vinifera C h r istia n K arl, G er h a r d M ü lle r un d P eter A lste d P e d e r se n W eleda A G , From m ethanolic extracts o f dried leaves o f Vitis vinifera 3 ellagitannins were isolated: brevila-gin 1, l,3-digalloyl-4,6-dehydrohexahydroxydiphenoylglucose (= vitilagin), 3,4-d igalloyl-l,6-de-hydrohexahydroxydiphenoylglucose (= iso-vitilagin). 
  Reference    Z. Naturforsch. 38c, 13 (1983); received September 28 1982 
  Published    1983 
  Keywords    Vitis vinifera L, Vitaceae, Tannins, Fast-Atom-Bombardement-M S 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0013.pdf 
 Identifier    ZNC-1983-38c-0013 
 Volume    38 
4Author    Z. NaturforschRequires cookie*
 Title    2-Methoxystypandron  
 Abstract    , ein neues Naphthochinon aus Rhamnus fallax B. 2-M ethoxystypandrone, a N ew N ap h th o q u in o n e from R ham nus fa lla x B. H ans-W . R au w ald und H olger M iething R ham nus fa lla x Boiss. (Oreoherzogia fa lla x Boiss.; R ha m nus alpinus L. ssp. fa lla x), 6-Acetyl-5-hydroxy-2-m ethoxy-7-m ethyl-l,4-naphthoquinone, 2-M ethoxystypandrone, Alatem in, 2-H ydroxyem odin 
  Reference    Z. Naturforsch. 38c, 17 (1983); received August 12 1982 
  Published    1983 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0017.pdf 
 Identifier    ZNC-1983-38c-0017 
 Volume    38 
5Author    Z. NaturforschRequires cookie*
 Title    Enzymatic Synthesis of Sinapine from l-O-Sinapoyl-ß-D-glucose and Choline by a Cell-Free System from Developing Seeds of Red Radish (Raphanus sativus L. var. sativus)  
 Abstract    1-O-Acyl G lucoside, H ydroxycinnamic Acid Conjugates, R aphanus, Sinapine, l-0-Sinapoyl-/?-D-G lucose: C holine Sinapoyltransferase A cell-free system from developing seeds o f red radish (R aphanus sativus L. var. sativus) catalyzed the transfer o f the sinapoyl m oiety o f l-0-sinapoyl-/?-D-glucose to choline to form O -sinapoylcholine (sinapine). A wide range o f proportionality with respect to incubation time and protein concentration was found in the determ ination o f this enzymatic activity using a high performance liquid chrom atographic (HPLC) method. The transacylase activity showed an absolute acceptor specificity towards choline and a high hydroxycinnamoyl donor specificity with 1-sinapoylglucose. M aximal rate o f sinapine formation was found to be around pH 7.0 in 0.08 M potassium phosphate buffer. The apparent K m values were 0.30 mM for 1-sinapoylglucose and 7.64 mM for choline. Change in extractable transacylase activities from different stages o f seed developm ent correlated well with the in vivo accum ulation kinetics o f sinapine. Maximal activity around 36 pkat seed-1 and 129 jakat kg-1 protein was extracted from dark-green immature seeds, which are in the process o f rapid sinapine accumulation. A bout 20% o f this activity still has been found in dark-brown mature seeds. This enzym atic acitivity refers to an enzyme which can be classified as 1-sinapoylglucose: choline sinapoyltransferase (SCT). 
  Reference    Z. Naturforsch. 38c, 21 (1983); received Septem ber 20 1982 
  Published    1983 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0021.pdf 
 Identifier    ZNC-1983-38c-0021 
 Volume    38 
6Author    E. Dith, E. Bert, W. In, M. Olfgang, K. Uecke, R. Laus, C. Am Steiner, Hristian VogelRequires cookie*
 Title    Inhibition of Ergosterol Biosynthesis by Etaconazole in Ustilago maydis  
 Abstract    The triazole fungicide etaconazole (C G A 6 4 251) interferes with the ergosterol biosynthesis in Ustilago m aydis by inhibiting the C-14 dem ethylation o f the sterol nucleus. During the late log growth phase o f U. m aydis a novel endogenous sterol m etabolite (14x-m ethyl-ergosta-8,24(28)-dien-3/?,6a-diol) was discovered and analyzed, which accumulates under the influence o f the fungicide. The structure o f this m etabolite points to a hydroxylation-dehydration m echanism for the introduction o f the double bond at C-5 during the ergosterol biosynthesis. In troduction 
  Reference    Z. Naturforsch. 38c, 28 (1983); received July 22 1982 
  Published    1983 
  Keywords    Fungal Lipids, Ergosterol, Inhibition, Etaconazole, Fungicide 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0028.pdf 
 Identifier    ZNC-1983-38c-0028 
 Volume    38 
7Author    E. M. Ilio, F. Ern, Á. Ndez, JacoboC.Requires cookie*
 Title    Isoelectric Focusing of the NAD(P)H-Cytochrome c Reductase Subunit of Chlamydomonas reinhardii Nitrate Reductase  
 Abstract    Isoelectric Focusing, Nitrate Reductase, Diaphorase, C hlam ydom onas reinhardii Chlam ydom onas reinhardii 104 mutant strain cells contain many N A D (P)H -cytochrom e c reductase activities unrelated to nitrate reduction in addition to an N A D (P)H -cytochrom e c reductase related to N A D (P)H -nitrate reductase, which have m olecular weights from 37000 to 124000. By com bining chromatographic procedures and preparative isoelectric focusing, three major fractions o f N A D (P)H -cytochrom e c reductase were isolated with pi at 7.1, 5.5 and 5.05, respectively. O f these fractions, only the last one was identified as the diaphorase subunit o f the NA D(P)H -nitrate reductase by its ability to reconstitute in vitro the nitrate reductase native com plex upon com plem entation with active terminal nitrate reductase o f 305 mutant o f C. reinhardii. In tro d uction 
  Reference    Z. Naturforsch. 38c, 35 (1983); received August 2/O ctober 7 1982 
  Published    1983 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0035.pdf 
 Identifier    ZNC-1983-38c-0035 
 Volume    38 
8Author    V. Iktor, P. B. Aryshok, D. Evard, I. Stom, D. Ietrich, W. Erner, M. Ichail, G. V. OronkovRequires cookie*
 Title    Wirkung von Silatranen auf Wachstum, Sauerstoffentwicklung und Dunkelatmung von Cyclotella cryptica (Diatomeae)  
 Abstract    T he Effect o f S ilatranes on G row th, P hotosynthetic O xygen E volution an d D ark R espiration o f Cyclotella cryptica (D iatom eae) R ichard R oth The effect o f 5 silatranes RSi (OCH 2 CH2)3N , where R = C 2 H 50 ; C6H 5; C1CH2 and R Si[O C H (C H 3)C H 2 ]3N , where R = C6H5, C1CH2 and tetraethoxysilane were studied on the growth, photosynthetic oxygen evolution and dark respiration o f the marine centric diatom C yclotella cryptica. Both silica-free and Si(O H) 4 supplem ented m edia were used. Only 1-ethoxy-silatrane was hydrolyzed by the diatom , giving sufficient quantities o f Si(O H) 4 to m aintain growth without exogenous Si(O H)4. In this case the diatom s m ultiplied by a factor o f 18 within 48 h. W ith silica in the m edium , 1-phenylsilatrane, 1 -phenyl-3,7,10-trim ethylsilatrane, 1 -chlormethylsilatrane and 1-chlorm ethyl-3,7 ,10-trimethylsilatrane exhibited only slightly inhibitory activity. In contrast, however, tetraethoxysilane (5 • 10-4 mol/1) inhibited growth almost com pletely. Respiration was unaffected by all the silatranes tested, but net photosynthetic oxygen evolution was reduced by approx. 50% by 1-chlorm ethylsilatrane and 1-chlormethyl-3,7,10-trimethylsilatrane and almost com pletely by 1-phenylsilatrane and 1 -phenyl-3,7,10-tri-methylsilatrane. In the absence o f silica as nutrient in the m edium , the silatranes hardly affected dark respiration within 24 h. Conversely, the same samples showed no photosynthetic oxygen evolution after 24 h, and 24 h later again a negative 0 2 balance was produced in the light. Different silatranes produced significant differences in the final oxygen balance. 
  Reference    Z. Naturforsch. 38c, 39 (1983); received September 3 1982 
  Published    1983 
  Keywords    Cyclotella cryptica, Silatranes, Inhibition o f Photosynthesis, Inhibition o f Dark Respiration 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0039.pdf 
 Identifier    ZNC-1983-38c-0039 
 Volume    38 
9Author    S. Achio, M. Iyairi, N. Th, H. SchatzRequires cookie*
 Title    Oxygen-Evolving Extracts from a Thermophilic Cyanobacterium Synechococcus sp  
 Abstract    Spheroplast membranes o f a thermophilic cyanobacterium Synechococcus sp. have been treated with the detergent lauryldim ethylam ine oxide (LD A O). The resulting extracts show (1) light-induced 0 2 evolution with artificial electron acceptors, (2) four-fold enhancement o f the 0 2 evolution relative to chlorophyll, (3) parallel increase o f both the molar ratios o f PS 2/C hl and cyt b559/C hl in the extract, (4) dissociation o f the auxiliary pigm ent phycocyanin upon treatment with LDAO, but still tight association o f allophycocyanin to the photosystem 2 preparation. 
  Reference    Z. Naturforsch. 38c, 44 (1983); received July 2/Septem ber 13 1982 
  Published    1983 
  Keywords    Cyanobacteria, Oxygen Evolution, Photosystem 2, Allophycocyanin, Energy Transfer 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0044.pdf 
 Identifier    ZNC-1983-38c-0044 
 Volume    38 
10Author    G. O., G. S. Chäfer, H. S. Tro, Tm AnRequires cookie*
 Title    Synthesis of 2'-/3'-0-Acylated Adenine Nucleotide Analogs and Their Interactions in Photophosphorylation  
 Abstract    By mono esterification o f 3'(2')-hydroxyl residues o f adenine nucleotides with various carb-oxylic acids a series o f nucleotide analogs is available including fluorescent and photoaffinity labels. Their chemical synthesis is described. The equilibrium between 2' and 3' esters is determined by N M R spectroscopy, stability o f the esters and their tendency o f acyl migration is discussed. The interaction o f the A D P derivatives with the chloroplast ATP synthesizing system is in­ vestigated. Actually, the analogs are typical energy transfer inhibitors, strongly inhibiting photo­ phosphorylation and concom itant coupled electron transport (ci50 values ranging from 0.3 to 85 |iM). On the basis o f inhibitory activities o f analogs bearing varying 3'-(2')-substituents, struc-ture-activity relationships are discussed. The inhibitory properties o f the em ployed A D P analogs are based on their specific interaction with the catalytic AD P binding site o f CF] and their extrem ely slow phosphorylation on the enzyme (rate 0.25% or less com pared to A D P phosphorylation). Inhibition is com petitive to AD P but non-com petitive with regard to P; . It is specific for the A D P derivatives, whereas the corresponding ATP analogs are only weak inhibitors in phosphorylation and the AMP derivatives are com pletely inactive. In light-triggered ATP hydrolysis, however, the ATP analogs exhibit an even stronger com petitive inhibition than the A D P derivatives. The results suggest that a conformational change o f ATPase takes place when the chloroplasts are transferred from energized to de-energized conditions which greatly affects the properties o f the active site with respect to nucleotide binding. In tro d u c tio n 
  Reference    Z. Naturforsch. 38c, 49 (1983); received October 7 1982 
  Published    1983 
  Keywords    N ucleotide Analogs, Photophosphorylation, Energy Transfer Inhibition 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0049.pdf 
 Identifier    ZNC-1983-38c-0049 
 Volume    38 
11Author    GeorgH. Schmid, Pierre ThibaultRequires cookie*
 Title    Studies on the S-State Distribution in Euglena gracilis  
 Abstract    When Euglena gracilis is dark adapted for 10 min or more, oxygen evolution as the conse­ quence of short (5 usee) saturating light flashes does not show the picture of a damped oscillation with a periodicity o f 4, as known from the literature. The overall picture of this flash pattern is given by the fact that 0 2-evolution in the First two flashes is practically zero and rises from there onward in a continuous manner to the steady state with barely any visible oscillation at all. However, a second flash sequence fired one to two minutes after this first sequence induces an oxygen evolution pattern which is barely distinguishable from the well known usual Chlorella vulgaris pattern. The phenomenon is not influenced by changes in the oxygen tension nor do additions of chemicals like CCCP, sodium azide, or reducing agents like hydroxylamine or hydrogen peroxide substantially alter the described behavior. Deactivation experiments give the overall impression that the deactivation of the S-states is slower than with Chlorella. Hydroxylamine strongly accelerates the deactivation. The analysis of the S-state distribution in a four and five state Kok-model suggests that dark adapted Euglena is in a more reduced condition than dark adapted Chlorella. It looks as if dark adapted Euglena were in a condition which would correspond to 60 percent S_|, 30 percent S0 and 10 percent S). The experimental flash sequence of such dark adapted cells fits best a synthetic sequence when the misses are in the region of 2 0 -2 5 percent, with double hitting playing practically no role at all (the first two flashes are zero!). The impression that dark adapted Euglena starts its oxygen evolution from a more reduced state is strengthened by the analysis of room temperature fluorescence induction (Kautsky effect). It can be shown that the fluorescence induction curve o f Euglena corresponds to that of Chlorella cells provided the latter have been briefly treated with a strong reductant such as sodium dithionite. 
  Reference    Z. Naturforsch. 38c, 60—6 (1983); received September 1982 
  Published    1983 
  Keywords    Flash Yield, Oxygen, Euglena, Fluorescence Induction 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0060.pdf 
 Identifier    ZNC-1983-38c-0060 
 Volume    38 
12Author    Jacek Wierzchowski, David ShugarRequires cookie*
 Title    Sensitive Fluorimetric Assay for Adenosine Deaminase with Formycin as Substrate; and Substrate and Inhibitor Properties of some Pyrazolopyrimidine and Related Analogues  
 Abstract    The nucleoside antibiotic formycin, 7-amino-3-(/?-D-ribofuranosyl)pyrazolo(4,3-d)pyrimidine, a structural analogue of adenosine, is deaminated about 10-fold faster by adenosine deaminase than adenosine itself, and is therefore a superior substrate for both routine assays and kinetic studies with the purified enzyme. The luminescence properties o f formycin have been profited from to develop a fluorimetric assay for adenosine deaminase which is considerably more sensi­ tive than the spectrophotometric procedure widely employed with adenosine as substrate. Exam­ ples are presented of its application to routine assays of adenosine deaminase levels in cellular extracts, as well as to kinetic studies with the purified enzyme, including the properties o f some pyrazolopyrimidine and purine substrates and inhibitors. 
  Reference    Z. Naturforsch. 38c, 67—7 (1983); received August 23 1982 
  Published    1983 
  Keywords    Formycin, Pyrazolopyrimidines, Adenosine Deaminase, Fluorimetric Assay, Inhibitors 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0067.pdf 
 Identifier    ZNC-1983-38c-0067 
 Volume    38 
13Author    R. Maschler, C. J. Smith, J. C. Allen, H. R. MaurerRequires cookie*
 Title    Elucidation of the Different Effects of Polyamines and Other Naturally-Occurring Inhibitors of Cell Proliferation (Chalones) on T-Lymphocyte and Granulocyte Colony Growth in vitro  
 Abstract    Using T-lymphocyte and granulocyte colony assays with truly proliferating cells the effects of the polyamine spermine and o f other naturally-occurring inhibitors of cell proliferation have been differentiated. It has been confirmed that spermine, in the presence o f fetal calf serum, is a potent inhibitor of cell proliferation. This inhibition could be reversed by the addition o f either 3-hydroxybenzyl-oxyamine or 4-bromo-3-hydroxybenzyl-oxyamine, both of which are inhibitors of the polyamine oxidase. In comparison, fractions isolated from calf thymus were shown to inhibit lymphocyte, but not granulocyte colony growth, indicating their tissue specificity and lymphocyte chalone activity. Further this inhibition was not reversed by polyamine oxidase inhibitors demonstrating that polyamines were not the inhibitory principles in this preparation. 
  Reference    Z. Naturforsch. 38c, 74—7 (1983); received February 24/August 24 1982 
  Published    1983 
  Keywords    Polyamines, Lymphocyte Responsiveness, Polyamine Oxidase Inhibitors, Lymphocyte Chalone 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0074.pdf 
 Identifier    ZNC-1983-38c-0074 
 Volume    38 
14Author    Minoru Kumakura, Isao KaetsuRequires cookie*
 Title    Effect of Organic Solvents on Enzymatic Hydrolysis of Celluloses  
 Abstract    Effect of organic solvents on enzymatic hydrolysis of celluloses was studied. It was found that toluene and xylene can accelerate the rate of the enzymatic hydrolysis reaction of filter paper and cellulose powder. The addition o f N,N-dimethylformamide, acetone, and alcohols was negative effect for the enzymatic hydrolysis o f solid celluloses. In the addition o f alcohols and glycols, the relative glucose yields increased with increasing the carbon number o f their solvents. The addition o f organic solvents on the enzymatic hydrolysis of celluloses was discussed with swelling effect. It was found that the glucose yield increased as the dipole moment o f the solvents decreased. 
  Reference    Z. Naturforsch. 38c, 79 (1983); received June 8/August 23 1982 
  Published    1983 
  Keywords    Organic solvent, Enzymatic Hydrolysis, Cellulose, Acceleration Effect, Glucose Yield 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0079.pdf 
 Identifier    ZNC-1983-38c-0079 
 Volume    38 
15Author    A. Andreoni, R. Cubeddu, S. De Silvestri, G. Jori, P. Laporta, E. ReddiRequires cookie*
 Title    Time-Resolved Fluorescence Studies of Hematoporphyrin in Different Solvent Systems  
 Abstract    Time-Resolved Fluorescence, Hematoporphyrin, Porphyrin Aggregates Time-resolved fluorescence studies o f hematoporphyrin in aqueous solution and in different organic solvents are presented. The observation of two exponential components in the fluores­ cence decay in aqueous solution reveals the presence o f a monomeric and aggregated (probably dimeric) form of the molecule, as confirmed by absorption and fluorescence measurements. The slow component (~ 15 ns in aqueous solution) and the fast one (~ 3.8 ns in aqueous solution) are attributed to monomers and dimers, respectively. Higher aggregated species of hematoporphyrin are also present, which are essentially devoid o f fluorescence properties. 
  Reference    Z. Naturforsch. 38c, 83 (1983); received June 3/September 24 1982 
  Published    1983 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0083.pdf 
 Identifier    ZNC-1983-38c-0083 
 Volume    38 
16Author    W. Lohmann, D. Holz, B. Kiefer, D. SchmidtRequires cookie*
 Title    On the Origin of the Non-Haemic Iron Transferrin ESR Signal: ESR Investigations on Histidine-Iron-Ascorbic Acid Systems  
 Abstract    The nature of the ferric high spin iron complex located at g = 4.3 has been investigated by means of electron spin resonance spectroscopy. It could be shown that the iron is bound to two histidines, three ascorbic acids, and one bicarbonate. This agrees well with previous Findings according to which the ligand field o f iron is composed mainly of oxygen and nitrogen atoms. Another low-field signal located at g = 9.5 appears always concomitantly with the g = 4.3 signal. It should be due, therefore, to a transition between the two sublevels o f the low-lying Kramers doublet in one principal direction. 
  Reference    Z. Naturforsch. 38c, 90—93 (1983); received June 28/October 1 1982 
  Published    1983 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0090.pdf 
 Identifier    ZNC-1983-38c-0090 
 Volume    38 
17Author    Enrico Boccù, Roberta Largajolli, FrancescoM. VeroneseRequires cookie*
 Title    Coupling of Monomethoxypolyethyleneglycols to Proteins via Active Esters  
 Abstract    Two alternative methods for the attachment of monomethoxypolyethyleneglycols (PEG) to proteins are proposed; they are based upon the replacement of the hydroxy terminal function of PEG to carboxylate followed by its activation with dicyclohexylcarbodiimide and N-hydroxy-succinimide. The methods, which give more homogeneous product than that employing trichloro-s-triazine as coupling reagent, may also be used for the modification of essential — SH containing enzymes. The attachment of PEG activated via esters was tested with several model proteins and the influence of the extent o f modification i. on the biological activity o f various enzymes, ii. on the binding capacity for albumin and iii. on the clearance time in rats using superoxide dismutase as model tracer was evaluated. It was also demonstrated that the extent of PEG attachment varies greatly according to the different proteins used. 
  Reference    Z. Naturforsch. 38c, 94 (1983); received July 7/September 29 1982 
  Published    1983 
  Keywords    Protein Surface Modification, Monomethoxypolyethyleneglycols Activation, PEG, Enzyme Therapy, Active Esters 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0094.pdf 
 Identifier    ZNC-1983-38c-0094 
 Volume    38 
18Author    Komei Washino, Otmar Denk, Wolfram SchnabelRequires cookie*
 Title    O H Radical-Induced Main-Chain Scission of Poly(Ribonucleic Acids) under Anoxic Conditions  
 Abstract    Poly(ribonucleic acids), poly A, poly C and poly U, were irradiated in 0 2-free dilute aqueous solution at pH 7 to 8 with single pulses (50 to 200 ns) of 16MeV electrons. With the aid of Rayleigh light scattering measurements main-chain scission, induced by OH radicals, was observed with the three polynucleotides. From the time dependence of the decrease o f the light scattering intensity (LSI), the existence of two modes of decrease was inferred, indicating at least two different chemical mechanisms were operative in main-chain degradation. Evidence for the assignment o f the slow mode of LSI decrease to the lifetime of a free radical was obtained from quenching experiments with cysteamine. It is noteworthy, that the extent and the lifetime o f LSI decrease are not the same for the three polynucleotides. The differences indicate the influence of the chemical nature o f the bases on main-chain scission. Consequently, it is concluded that OH attack at carbons in 1' and/or 2' position of the ribose moiety contributes essentially to the degradation mechanism. 
  Reference    Z. Naturforsch. 38c, 100 (1983); received August 17/November 11 1982 
  Published    1983 
  Keywords    Poly(Ribonucleic Acids), OH Attack, Chain Breakage, Free Radicals, Time-Resolved Light Scattering 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0100.pdf 
 Identifier    ZNC-1983-38c-0100 
 Volume    38 
19Author    Akira Taketo, Yoriko TaketoRequires cookie*
 Title    Effect of Streptococcal Extracellular Nuclease on the Carrier Activity of RNA for Streptolysin S  
 Abstract    Upon digestion with a streptococcal extracellular nuclease, yeast RNA yielded acid-insoluble core having increased carrier activity for streptolysin S. The carrier activity was found in minor fractions of the core which were eluted from a DEAE-cellulose column at higher salt concentrations. Upon gel filtration through a Sephadex G-75 column, the effective component (Fr. I) was eluted earlier than bulk oligonucleotides (Fr. II). Nucleotide composition (in mol %) of Fr. I was AMP: 21.8; GMP: 55.1; CMP: 8.2; UMP: 14.9, whereas that o f Fr. II was AMP: 38.0; GMP: 33.1; CMP: 8.0; UMP: 20.9. Chromatographic patterns of SLS complex induced by Fr. I were similar to those of the toxin formed in the presence o f active fraction prepared from RNase I core. Hemolytic activity of the latter complex was, like the former, unaffected by streptococcal nuclease treatment. The carrier activity o f DNA digested with the nuclease was also investigated. 
  Reference    Z. Naturforsch. 38c, 107 (1983); received October 1 1982 
  Published    1983 
  Keywords    Streptolysin S, RNA Effect, Extracellular Nuclease, Streptococcus hemolyticus 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0107.pdf 
 Identifier    ZNC-1983-38c-0107 
 Volume    38 
20Author    Franz Römer, Ilona EisenbeisRequires cookie*
 Title    DNA Content and Synthesis in Several Tissues and Variation of Moulting Hormone-Level in Gryllus bimaculatus DEG (Ensifera, Insecta)  
 Abstract    The mode of growth o f several tissues in Gryllus bimaculatus was investigated during postembryonic development by cytophotometric methods. In contrast to the situation in holo-metabolous insects, the tissues growing by endomitosis reach only moderate levels of polyploidy. In this case the growth o f tissues is achieved by mitotic divisions o f small cells with subsequent polyploidization. The time courses of D NA synthesis were measured within the 3rd and, for comparison, the 8th larval instar by incorporation o f labelled thymidine followed by autoradiography. Hemocytes, cells of the regeneration crypts of the midgut, gonads and nervous tissue showed a continuous incorporation rate; by contrast, DNA synthesis in other tissues was confined to a given time within the moulting cycle. The changes in moulting hormone titre o f the 3rd larval instar were investigated. The quantities of ecdysone and 20-C)H-ecdysone were estimated by radioimmunoassay, and the hormones were identified by high pressure liquid chromatography (HPLC). The titre changes in time with at least 2 distinct maxima. The DNA synthesis periods are correlated with the hormone peaks, that o f epidermis and tracheae with the first peak, and that of pylorus, ileum, rectum, Malpighian tubules and fat body with the second. DNA synthesis in prothoracic glands and oenocytes exhibits a time course that is the inverse of the hormone-secretion time course. The question whether moulting hormones have an influence on D NA synthesis is discussed. 
  Reference    Z. Naturforsch. 38c, 112 (1983); received June 28/September 16 1982 
  Published    1983 
  Keywords    Polyploidy Levels, DNA Synthesis Development, Ecdysteroids, Gryllus bimaculatus 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0112.pdf 
 Identifier    ZNC-1983-38c-0112 
 Volume    38 
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