| | 1 | Author
| Uwe Murawski, Heinz Egge, Paul György, Fritz Zilliken | Requires cookie* | | | Title
| Nicht methylenunterbrochene «V/y-Octadecadiensäuren Minor Constituents of Human Milk, VI Non Methylene Interrupted cz's,cw-Octadecadienoic Acids  | | | | Abstract
| Human M ilk, Octadecadienoic Acids, Hydroxylation, Trimethylsilylation The non methylene interrupted cis,cis-octadecadienoic acids from human milk fat were pre pared by thin layer chromatography on A g N 0 3 impregnated silicagel plates. The position of double bonds was determined by hydroxylation of the double bonds with 0 s 0 4 followed by silylation. The corresponding O-trimethylsilylether derivatives of the fatty acid methyl esters were analyzed by gaschromatography mass spectrometry. 14 isomers of the following series could be identified: | | | |
Reference
| (Z. Naturforsch. 29c, 1 [1974]; eingegangen am 5. Juli 1973) | | | |
Published
| 1974 | | | |
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| default:Reihe_C/29/ZNC-1974-29c-0001.pdf | | | | Identifier
| ZNC-1974-29c-0001 | | | | Volume
| 29 | |
| 3 | Author
| M. Polverelli, R. Teoule | Requires cookie* | | | Title
| Identification of Radiolysis Products of Cytosine Resulting from the Deamination Pathway | | | | Abstract
| Gamma Irradiation, Cytosine, Radioproducts, Identification Radiolysis products resulting from the deamination pathway were isolated and identified after irradiation of cytosine by gamma rays in neutral aqueous solutions. They were identical to those identified in uracil radiolysis i. e. 5,6-dihydroxy-5,6-dihydro-uracil (cis and trans forms), iso-dialuric acid, alloxan, 5-hydroxy-hydantoin, parabanic acid and oxaluric acid. The absence of 4,4'-di-iso-barbituric acid, alloxantin or iso-barbituric acid could be explained by the good stability of uracil glycols. The identification of buiret proof of potential degradation mechanisms different from these involving of the free radical attack on the 5,6 double bond. Gamma Irradiation of Cytosine in an Aerated Aqueous Solution, I | | | |
Reference
| (Z. Naturforsch. 29c, 12 [1974]; received July 9 1973) | | | |
Published
| 1974 | | | |
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| default:Reihe_C/29/ZNC-1974-29c-0012.pdf | | | | Identifier
| ZNC-1974-29c-0012 | | | | Volume
| 29 | |
| 5 | Author
| KlausP. Hofmann, Georg Zundel | Requires cookie* | | | Title
| Stepwise Protonation of P 0 43 , ADP and ATP Salts, IR Investigations | | | | Abstract
| ATP. Energy-Rich Phosphate, Hydrolysis Products of ATP, Proton Addition, Hydrogen Bonds Polarizable IR spectra of aqueous solutions of P 0 43-, A D P 3-, ATP4-, Mg AD P-and (MgADP~ + P 0 43_) (in two cases hydrated layers) were plotted. The parameter for the investigation was the mole percent of protons relative to the anions. The other cations were sodium or potassium. The purpose of our work was to obtain a basis for understanding the results regarding the changes to the interactions in ATP systems on hydrolysis. The addition of protons to the different acceptors is discussed on the basis of the bands of the phosphate groups and of the base ring vibrations. The formation of easily polarizable hydrogen bonds could be demonstrated with the aid of an IR continuum. Such 0 H +-*, 0 bonds between hydrogen phosphate ions as well as N H+>,-N bonds form in the systems investigated. Thus the conditions for forming such bonds and the resulting intermolecular interactions will become evident in this work. | | | |
Reference
| (Z. Naturforsch. 29c, 19 [1974]; received July 10 1973) | | | |
Published
| 1974 | | | |
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| default:Reihe_C/29/ZNC-1974-29c-0019.pdf | | | | Identifier
| ZNC-1974-29c-0019 | | | | Volume
| 29 | |
| 6 | Author
| KlausP. Hofmann, Georg Zundel | Requires cookie* | | | Title
| Effect of the Protons Arising in Aqueous Hydrolysing ATP Solutions, IR Investigations | | | | Abstract
| IR spectra of 0.3 m aqueous solutions of Mgo.5K3_ nH w ATP were plotted. The dependence of the spectra on the hydrolysis was investigated for three different K/H ratios of the nonhydrolysed system. Changes to bands provide information as to the protons added to the phosphate groups and base residues as a function of the initial protonation and degree of hydrolysis. More and more proton addition to the — P 0 3__ groups is observed as the initial protonation increases and as the degree of hydrolysis rises. The same applies as far as the addition of protons to the base residues is concerned to the systems with a higher initial protonation. At low degrees of hydrolysis the P 0 43' ions which occur do not bind the two hydrolysis protons completely. 150% protonated P 0 43" ions first form which cross-like via 0 H +--'0 hydrogen bonds, which only become more strongly protonated in the systems with a higher initial protonation at large degrees of hydrolysis. The 0 H +---0 bonds between the 150% protonated hydrogen phosphate ions as well as the N H +,--N hydrogen bonds between the base residues cause an IR continuum and are thus easily polarizable. An extremely large change to the hydrate structure within narrow hydrolysis ranges is indicated by changes to the bands of the water molecules. The biological relevance of these findings is briefly discussed in the conclusions. Intermolecular Interaction in Systems with Energy-Rich Phosphates, II | | | |
Reference
| (Z. Naturforsch. 29c, 29—35 [1974]; received July 10 1973) | | | |
Published
| 1974 | | | |
Keywords
| Energy-Rich Phosphate, ATP Hydrolysis, Hydrogen Bonds Polarizable, Intermolecular Interaction, Proton Addition | | | |
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| default:Reihe_C/29/ZNC-1974-29c-0029.pdf | | | | Identifier
| ZNC-1974-29c-0029 | | | | Volume
| 29 | |
| 7 | Author
| Albrecht Maurer, Wolfgang Engelmann | Requires cookie* | | | Title
| Effect of D 20 on the Circadian Rhythm of Petal Movement of Kalanchoe | | | | Abstract
| Heavy Water, Circadian Rhythm, Petal Movement, Kalanchoe D 20 lengthens the free running period of the circadian petal movement of isolated Kalanchoe flowers by 1.6% per 10% heavy water. This corresponds to findings reported in the literature for other organisms. 100% D 20 administered in the form of 4 hour pulses at various phases of the circadian cycle lead to phase responses zero to maximally 1.5 hour delays. No advancing phase shifts occur. Possible ways in which lengthening of the period occurs are discussed. | | | |
Reference
| (Z. Naturforsch. 29c, 36 [1974]; received October 23 1973) | | | |
Published
| 1974 | | | |
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| default:Reihe_C/29/ZNC-1974-29c-0036.pdf | | | | Identifier
| ZNC-1974-29c-0036 | | | | Volume
| 29 | |
| 9 | Author
| Dieter Plesse, Manfred Liidicke | Requires cookie* | | | Title
| Die radioaktive Markierung des Melaninpigments der Fischhaut mit [35S]Natriumsulfat Radioactive Labelling of the Melanin Pigment in Fish Skin with [35S] Sodium Sulphate | | | | Abstract
| Melanins, Melanomas, [35S] Sodium Sulphate [35S] Sodium sulphate was intraperitoneally injected into adult fishes and its incorporation into melanins examined. Relevant incorporation of the tracer into normal skin and peritoneal melano cytes of Poecilia reticulata (Peters) and Puntius tetrazona tetrazona (Hamilton) could be detected only in rare cases. Melanocytes of malignant melanomas in the hybrids of Xiphophorus helleri (Heckel) and Platypoecilus maculatus (Guenther) exhibited a different degree of radioactivity ac cording to their degree of differentiation. The isolation of the melano-protein complex from melanocytes of these melanomas and its subsequent hydrolysis resulted in the following: During melanin synthesis, [35S] sulphate had been incorporated into the chromophore group as an ester-sulphate, whereas no significant incorporation of the tracer was found in the protein matrix. | | | |
Reference
| (Z. Naturforsch. 29c, 42 [1974]; eingegangen am 30. Juli/12. November 1973) | | | |
Published
| 1974 | | | |
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| default:Reihe_C/29/ZNC-1974-29c-0042.pdf | | | | Identifier
| ZNC-1974-29c-0042 | | | | Volume
| 29 | |
| 10 | Author
| Hans Eckstein | Requires cookie* | | | Title
| DNA Turnover in Growing Yeast after X-Irradiation | | | | Abstract
| DNA Turnover, X-Irradiation, Cycloheximide, Yeast Growing yeast cells incorporate radioactivity from labeled nucleotides, deoxynucleotides, deoxy-adenosine, and deoxycytidine into acid-insoluble material. Most of these compounds are in corporated predominantly into RNA, only dTMP and dTTP serve as effective and selective DNA precursors. When yeast is prelabeled with dTMP, "specific" radioactivity of D N A decreases in the growing cells due to DNA augmentation. In X-irradiated yeast, "specific" radioactivity of DNA again de creases remarkably, though DNA augmentation of the growing giant cells in delayed. Simultaneous ly, radioactivity is rendered acid-soluble. Vice versa, X-irradiated unlabeled yeast incorporates radioactivity from labeled precursors into DNA, even if the DNA content of the cells does not increase. When growing yeast cells are treated with cycloheximide, protein synthesis and DNA augmen tation are inhibited completely with 5-10~"6 m cycloheximide. The decrease of "specific" radio activity of DNA in X-irradiated cells, however, proves independent on an intact protein synthesis, even with 1 • 10—4 M cycloheximide. The data provide clear evidence on DNA turnover in growing yeast after X-irradiation. | | | |
Reference
| (Z. Naturforsch. 29c, 48 [1974]; received October 8 1973) | | | |
Published
| 1974 | | | |
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| default:Reihe_C/29/ZNC-1974-29c-0048.pdf | | | | Identifier
| ZNC-1974-29c-0048 | | | | Volume
| 29 | |
| 11 | Author
| Theodor Günther, Wolfgang Pellnitz, Gert Mariß | Requires cookie* | | | Title
| Effect of Salts on the Activity and Inhibition of E. coli Membrane ATPase by Ethacrynic Acid and Inhibitors | | | | Abstract
| Chaotropic Anions, E. coli ATPase, Ethacrynic Acid The [Mg, Ca] -ATPase activity of E. coli depends on the anion present and follows the chao tropic sequence: Acetate" > H C 0 3~ > Cl-> I " > N 0 3~ > SCN". There are only small differences between the different alkali chlorides. The [Mg, Ca]-ATPase was inhibited by all these salts when the ratio of Mg or Ca to ATP was 1: 5 at pH 9.1. At pH 9.1 or 7.5 and a Mg to ATP ratio of 1 some salts activate and others inhibit the enzyme according to their position in the chaotropic sequence. W ith Ca the ATPase was activated under these conditions only by acetate. The contradictory activation or inhibition by NaCl or KC1 reported by various authors are due to differences in the Mg: ATP ratios used. The inhibition of ATPase by ethacrynic acid, NEM, pCMB. DCCD and azide is much greater at pH 9.1 than at pH 7.5 and is drastically affected by NaCl or KC1. In E. coli an ATPase which was slightly activated | | | |
Reference
| (Z. Naturforsch. 29c, 54 [1974]; received September 25 1973) | | | |
Published
| 1974 | | | |
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| default:Reihe_C/29/ZNC-1974-29c-0054.pdf | | | | Identifier
| ZNC-1974-29c-0054 | | | | Volume
| 29 | |
| 12 | Author
| Theodor Günther, Gert Mariß | Requires cookie* | | | Title
| The Membrane ATPase of the (Mg, Ca)-ATPase-Mutant 12, strain AN 120 | | | | Abstract
| (Mg, Ca) -ATPase-Mutant The activity of the membrane ATPase of the (Mg, C a)-ATPase-mutant E. coli K 12 strain AN 120 is reduced to about 5 — 50% of the wildtype ATPase. The reduction is greatest at pH 9.1 and a ratio of Mg, or Ca to ATP of 1 :5 , and smallest at pH 7.5 and 9.1 and a ratio of Mg or Ca to ATP of 1 or more. | | | |
Reference
| (Z. Naturforsch. 29c, 60 [1974]; received September 25 1973) | | | |
Published
| 1974 | | | |
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| default:Reihe_C/29/ZNC-1974-29c-0060.pdf | | | | Identifier
| ZNC-1974-29c-0060 | | | | Volume
| 29 | |
| 13 | Author
| Wolfgang Köhnlein | Requires cookie* | | | Title
| Transforming Activity in Both Complementary Strands of Bacillus subtilis DNA | | | | Abstract
| Transforming Activity, Ultracentrifugation, BrUdR-Sensitization A Bacillus subtilis mutant (strain 168 try" thy-) with a thymine-adenine bias in complementary DNA strands was found. After BrU-incorporation into the DNA it was possible to separate physi cally hybrid DNA-molecules BrU-labeled in different complementary strands using CsCl density gradient centrifugation. These hybrid DNA preparations were used to investigate whether both strands can be informative during transformation. Taking advantage of the selective degradation of BrU-labeled DNA strands by long wavelength UV it was found that the loss of transforming acti vity was the same for both hybrid DNA preparations. Thus indicating that both complementary DNA strands can be genetically active during transformation. These results are supported by trans formation experiments with denatured and reannealed DNA-molecules and heteroduplex DNA-molecules. | | | |
Reference
| (Z. Naturforsch. 29c, 63 [1974]; received October 29 1973) | | | |
Published
| 1974 | | | |
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| default:Reihe_C/29/ZNC-1974-29c-0063.pdf | | | | Identifier
| ZNC-1974-29c-0063 | | | | Volume
| 29 | |
| 15 | Author
| B. Dietzschold, 0. R. Kaaden, S. Ueberschaer, F. Weiland, 0. C. Straub | Requires cookie* | | | Title
| Suggestive Evidence for an Oncorna-Virus-Specific DNA Polymerase from C-Type Particles of Bovine Leukosis | | | | Abstract
| Bovine Leukosis, C-Particles, DNA-Polymerase Typical C-type oncorna virus particles as shown by electron microscopy have been purified from the supernatant of cultured lymphocytes from bovine leukosis. In the purified C-particle fraction a DNA-polymerase activity was detected. Using several synthetic RNA-or DNA-homopolymers and 70S Friend virus R N A the template response of this bovine leukosis cell particle DNA polymerase was compared with those of feline leukaemia virus DNA polymerase and DNA polymerase from normal bovine lymphocytes. The DNA polymerase detected in the viral preparation of bovine leukosis is suggested to be an oncorna-virus-specific enzyme. | | | |
Reference
| (Z. Naturforsch. 29c, 72 [1974]; received September 25/November 16 1973) | | | |
Published
| 1974 | | | |
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| default:Reihe_C/29/ZNC-1974-29c-0072.pdf | | | | Identifier
| ZNC-1974-29c-0072 | | | | Volume
| 29 | |
| 16 | Author
| V. Dostal, E. Schauenstein, P. Kulnigg, E. Schmeller | Requires cookie* | | | Title
| Der Einfluß von 4-Hydroxy-2.3-pentenal (HPE) auf die Vermehrung von Vaccinia-Virus in Hühnerfibroblastenkulturen Influence of 4-Hydroxy-2,3-pentenale (HPE) on the Multiplication of Vaccinia-virus in Chick-embryo Fibroblast Cultures | | | | Abstract
| Vaccinia-virus, Inhibition of DNA Synthesis, 4-Hydroxy-2,3-pentenale The influence of 4-hydroxy-2,3-/rans-pentenale (HPE) on multiplication of Vaccinia virus in chick fibroblast cultures has been investigated. The cytotoxicity of HPE has been estimated by in corporation of [3H] thymidine, [3H] uridine, [3H] leucine and by morphological tests. The greatest influence of H PE was seen after incorporation of [3H] thymidine. Chick-embryo cultures infected with Vaccinia virus showed a marked increase of DNA synthesis in contrast to the controls. This increase averaged 180% after 16 to 14 hours. HPE 0.05-10_ 3 M caused a reduction of 72% in [3H] thymidine incorporation in virus infected cultures. At the same time virus concentration decreased by 76%. HPE 0.1-10~3 M, a concentration which inhibits the normal cell DNA synthesis by 50%, caused about 90% inhibition in infected cultures; virus m ulti plication was 99% inhibited. These findings have been attributed to a selective inhibition of virus DNA synthesis by HPE. and possible mechanisms are discussed. | | | |
Reference
| (Z. Naturforsch. 29c, 76 [1974]; eingegangen am 2. Januar/30. Juli 1973) | | | |
Published
| 1974 | | | |
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| default:Reihe_C/29/ZNC-1974-29c-0076.pdf | | | | Identifier
| ZNC-1974-29c-0076 | | | | Volume
| 29 | |
| 17 | Author
| J. Hölzl | Requires cookie* | | | Title
| Bildung und [14C]-Markierung der | | | | Abstract
| Flavono lignane (Silymarin) bei S ily b u m m a r ia n u m (Haller) Biosynthesis and [14C] Labelling of Flavonolignans (Silymarin) by Silybum marianum (Haller) Flavonolignans, Silymarin Die Früchte von Silybum marianum (Haller) ent halten ein Flavonoidgemisch, aus dem bisher neben Taxifolin die drei Flavonolignane Silybin1, Sily-dianin2 und Silychristin3 isoliert und in ihrer Struktur aufgeklärt wurden. Flavonolignane sollen entsprechend ihrer Struktur durch Kondensation aus Taxifolin und Coniferylalkohol entstehen. Pro blematisch für Biosyntheseuntersuchungen was bis her die Pflanzengröße von 2 bis 3 m. Durch Kulti vierung unter besonderen Gewächshausbedingungen erhielt man Pflanzen zwischen 30 und 50 cm Höhe. Obwohl bei den Gewächshauspflanzen die Zeitdauer der Fruchtbildung gegenüber Pflanzen im Freiland verlängert und das Gesamtgewicht der Früchte pro Blütenstand vermindert war, war der Gehalt der Flavonolignane und ihre prozentuale Zusammen setzung nicht beeinträchtigt. | | | |
Reference
| (Z. Naturforsch. 29c, 82 [1974]; eingegangen am 28. November 1973) | | | |
Published
| 1974 | | | |
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| default:Reihe_C/29/ZNC-1974-29c-0082_n.pdf | | | | Identifier
| ZNC-1974-29c-0082_n | | | | Volume
| 29 | |
| 18 | Author
| KrishnaC. Joshi, R. K. Bansal, Pahup Singh | Requires cookie* | | | Title
| | | | | Abstract
| We have earlier reported the isolation of lapachol, dehydrotectol, dehydro-a-lapachone, /Mapachone, tectol, /5-sitosterol and paulownin along with d-sesamin from the petroleum ether extract of the heartwood of Phyllarthron comorense DC 1. Paulow | | | |
Reference
| (Z. Naturforsch. 29c, 84 [1974]; received July 9 1973) | | | |
Published
| 1974 | | | |
Keywords
| Paulownin, Phyllarthron Comorense, Mass Spectrum | | | |
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| default:Reihe_C/29/ZNC-1974-29c-0084_n.pdf | | | | Identifier
| ZNC-1974-29c-0084_n | | | | Volume
| 29 | |
| 19 | Author
| Heinz Faulstich, Dionysos Georgopoulos, Maria Bloching, Theodor Wieland | Requires cookie* | | | Title
| Analysis of the Toxins of Amanitin-Containing Mushrooms | | | | Abstract
| We have recently developed a method for the analysis of toxins in single specimens of the mush room Amanita phalloides (Fr.) Seer.1. Separation of the different toxins was achieved by column chro matography, on Sephadex LH20 in water, of a methanolic extract of fresh or dried mushrooms, followed by further purification on thinlayers of silica gel, spectrophotometric evaluation, and, final ly, identification of the toxins by amino acid analy ses of their y-hydroxylated leucines or isoleucines as lactones. This procedure afforded the quantitative determination of up to 10 toxins in 2 samples of A. phalloides from different sources. | | | |
Reference
| (Z. Naturforsch. 29c, 86 [1974]; received October 25 1973) | | | |
Published
| 1974 | | | |
Keywords
| Amanitins, Phallotoxins, Toxic Mushrooms, Chromatography | | | |
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| default:Reihe_C/29/ZNC-1974-29c-0086_n.pdf | | | | Identifier
| ZNC-1974-29c-0086_n | | | | Volume
| 29 | |
| 20 | Author
| 0. Motl, L. Dolejš | Requires cookie* | | | Title
| Laserpitium siler L | | | | Abstract
| Laserpitium siler, 12-Nonacosanone, Mass Spectra During our study on the seed constituents of Laserpitium siler L. we have isolated some time ago a crystalline compound of m.p. 74.5 °C showing the frequency of 1706 cm" 1 in its infrared spec trum We have now identified this substance as 12-nonacosanone by mass spectrometry. The high resolution measurement gave for the molecular ion a value of 422.4493 which corresponded to the com position C29H580 (calculated 422.4487). The cleav age of the ionized molecule in the neighborhood of | | | |
Reference
| (Z. Naturforsch. 29c, 89 [1974]; received September 17 1973) | | | |
Published
| 1974 | | | |
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| default:Reihe_C/29/ZNC-1974-29c-0089_n.pdf | | | | Identifier
| ZNC-1974-29c-0089_n | | | | Volume
| 29 | |
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