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Facet   section ZfN Section C:Volume 029  [X]
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1974 (184)
1Author    Uwe Murawski, Heinz Egge, Paul György, Fritz ZillikenRequires cookie*
 Title    Nicht methylenunterbrochene «V/y-Octadecadiensäuren Minor Constituents of Human Milk, VI Non Methylene Interrupted cz's,cw-Octadecadienoic Acids  
 Abstract    Human M ilk, Octadecadienoic Acids, Hydroxylation, Trimethylsilylation The non methylene interrupted cis,cis-octadecadienoic acids from human milk fat were pre­ pared by thin layer chromatography on A g N 0 3 impregnated silicagel plates. The position of double bonds was determined by hydroxylation of the double bonds with 0 s 0 4 followed by silylation. The corresponding O-trimethylsilylether derivatives of the fatty acid methyl esters were analyzed by gaschromatography mass spectrometry. 14 isomers of the following series could be identified: 
  Reference    (Z. Naturforsch. 29c, 1 [1974]; eingegangen am 5. Juli 1973) 
  Published    1974 
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 TEI-XML for    default:Reihe_C/29/ZNC-1974-29c-0001.pdf 
 Identifier    ZNC-1974-29c-0001 
 Volume    29 
2Author    W. H. Bannister, J. V. BannisterRequires cookie*
 Title    Evidence for the Validity of Three-Component Fitting of Protein Circular Dichroism Spectra  
 Abstract    The validity of three-component fitting of protein circular dichroism (CD) spectra in the peptide absorption region with basis spectra for helical, /?-sheet and unordered structure is examined by matrix rank analysis of protein CD spectra. It is shown that a three-component basis can be justified for several proteins within the limits of experimental error of the spectra. It is suggested that the fitting of protein CD spectra with basis spectra derived from reference proteins should be preceded by matrix rank analysis to show that the CD spectra of the reference and unknown proteins have the same basis. 
  Reference    (Z. Naturforsch. 29c, 9 [1974]; received September 5 1973) 
  Published    1974 
  Keywords    Protein Circular Dichroism Spectra, Three-Component Basis, Matrix Rank Analysis, Curve Fitting 
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 TEI-XML for    default:Reihe_C/29/ZNC-1974-29c-0009.pdf 
 Identifier    ZNC-1974-29c-0009 
 Volume    29 
3Author    M. Polverelli, R. TeouleRequires cookie*
 Title    Identification of Radiolysis Products of Cytosine Resulting from the Deamination Pathway  
 Abstract    Gamma Irradiation, Cytosine, Radioproducts, Identification Radiolysis products resulting from the deamination pathway were isolated and identified after irradiation of cytosine by gamma rays in neutral aqueous solutions. They were identical to those identified in uracil radiolysis i. e. 5,6-dihydroxy-5,6-dihydro-uracil (cis and trans forms), iso-dialuric acid, alloxan, 5-hydroxy-hydantoin, parabanic acid and oxaluric acid. The absence of 4,4'-di-iso-barbituric acid, alloxantin or iso-barbituric acid could be explained by the good stability of uracil glycols. The identification of buiret proof of potential degradation mechanisms different from these involving of the free radical attack on the 5,6 double bond. Gamma Irradiation of Cytosine in an Aerated Aqueous Solution, I 
  Reference    (Z. Naturforsch. 29c, 12 [1974]; received July 9 1973) 
  Published    1974 
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 TEI-XML for    default:Reihe_C/29/ZNC-1974-29c-0012.pdf 
 Identifier    ZNC-1974-29c-0012 
 Volume    29 
4Author    M. Polverelli, R. TeouleRequires cookie*
 Title    Kinetic Study of the Formation of the Radiolysis Products of Cytosine Resulting from the Deamination Pathway  
 Abstract    Gamma irradiation, cytosine, kinetic 
  Reference    (Z. Naturforsch. 29c, 16 [1974]; received July 9 1973) 
  Published    1974 
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 TEI-XML for    default:Reihe_C/29/ZNC-1974-29c-0016.pdf 
 Identifier    ZNC-1974-29c-0016 
 Volume    29 
5Author    KlausP. Hofmann, Georg ZundelRequires cookie*
 Title    Stepwise Protonation of P 0 43 , ADP and ATP Salts, IR Investigations  
 Abstract    ATP. Energy-Rich Phosphate, Hydrolysis Products of ATP, Proton Addition, Hydrogen Bonds Polarizable IR spectra of aqueous solutions of P 0 43-, A D P 3-, ATP4-, Mg AD P-and (MgADP~ + P 0 43_) (in two cases hydrated layers) were plotted. The parameter for the investigation was the mole percent of protons relative to the anions. The other cations were sodium or potassium. The purpose of our work was to obtain a basis for understanding the results regarding the changes to the interactions in ATP systems on hydrolysis. The addition of protons to the different acceptors is discussed on the basis of the bands of the phosphate groups and of the base ring vibrations. The formation of easily polarizable hydrogen bonds could be demonstrated with the aid of an IR continuum. Such 0 H +-*, 0 bonds between hydrogen phosphate ions as well as N H+>,-N bonds form in the systems investigated. Thus the conditions for forming such bonds and the resulting intermolecular interactions will become evident in this work. 
  Reference    (Z. Naturforsch. 29c, 19 [1974]; received July 10 1973) 
  Published    1974 
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 TEI-XML for    default:Reihe_C/29/ZNC-1974-29c-0019.pdf 
 Identifier    ZNC-1974-29c-0019 
 Volume    29 
6Author    KlausP. Hofmann, Georg ZundelRequires cookie*
 Title    Effect of the Protons Arising in Aqueous Hydrolysing ATP Solutions, IR Investigations  
 Abstract    IR spectra of 0.3 m aqueous solutions of Mgo.5K3_ nH w ATP were plotted. The dependence of the spectra on the hydrolysis was investigated for three different K/H ratios of the nonhydrolysed system. Changes to bands provide information as to the protons added to the phosphate groups and base residues as a function of the initial protonation and degree of hydrolysis. More and more proton addition to the — P 0 3__ groups is observed as the initial protonation increases and as the degree of hydrolysis rises. The same applies as far as the addition of protons to the base residues is concerned to the systems with a higher initial protonation. At low degrees of hydrolysis the P 0 43' ions which occur do not bind the two hydrolysis protons completely. 150% protonated P 0 43" ions first form which cross-like via 0 H +--'0 hydrogen bonds, which only become more strongly protonated in the systems with a higher initial protonation at large degrees of hydrolysis. The 0 H +---0 bonds between the 150% protonated hydrogen phosphate ions as well as the N H +,--N hydrogen bonds between the base residues cause an IR continuum and are thus easily polarizable. An extremely large change to the hydrate structure within narrow hydrolysis ranges is indicated by changes to the bands of the water molecules. The biological relevance of these findings is briefly discussed in the conclusions. Intermolecular Interaction in Systems with Energy-Rich Phosphates, II 
  Reference    (Z. Naturforsch. 29c, 29—35 [1974]; received July 10 1973) 
  Published    1974 
  Keywords    Energy-Rich Phosphate, ATP Hydrolysis, Hydrogen Bonds Polarizable, Intermolecular Interaction, Proton Addition 
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 TEI-XML for    default:Reihe_C/29/ZNC-1974-29c-0029.pdf 
 Identifier    ZNC-1974-29c-0029 
 Volume    29 
7Author    Albrecht Maurer, Wolfgang EngelmannRequires cookie*
 Title    Effect of D 20 on the Circadian Rhythm of Petal Movement of Kalanchoe  
 Abstract    Heavy Water, Circadian Rhythm, Petal Movement, Kalanchoe D 20 lengthens the free running period of the circadian petal movement of isolated Kalanchoe flowers by 1.6% per 10% heavy water. This corresponds to findings reported in the literature for other organisms. 100% D 20 administered in the form of 4 hour pulses at various phases of the circadian cycle lead to phase responses zero to maximally 1.5 hour delays. No advancing phase shifts occur. Possible ways in which lengthening of the period occurs are discussed. 
  Reference    (Z. Naturforsch. 29c, 36 [1974]; received October 23 1973) 
  Published    1974 
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 TEI-XML for    default:Reihe_C/29/ZNC-1974-29c-0036.pdf 
 Identifier    ZNC-1974-29c-0036 
 Volume    29 
8Author    H. VeenRequires cookie*
 Title    Specificity of Phospholipid Binding to Indole Acetic Acid and Other Auxins  
 Abstract    Auxins, Phospholipids Studies in vitro on the binding of phospholipids with IAA, a-NAA and some of their biologi­ cally inactive analogues demonstrate that the observed interaction between IA A and lecithin is not related to the primary action of the hormone in plant growth. 
  Reference    (Z. Naturforsch. 29c, 39—41 [1974]; received November 5 1973) 
  Published    1974 
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 TEI-XML for    default:Reihe_C/29/ZNC-1974-29c-0039.pdf 
 Identifier    ZNC-1974-29c-0039 
 Volume    29 
9Author    Dieter Plesse, Manfred LiidickeRequires cookie*
 Title    Die radioaktive Markierung des Melaninpigments der Fischhaut mit [35S]Natriumsulfat Radioactive Labelling of the Melanin Pigment in Fish Skin with [35S] Sodium Sulphate  
 Abstract    Melanins, Melanomas, [35S] Sodium Sulphate [35S] Sodium sulphate was intraperitoneally injected into adult fishes and its incorporation into melanins examined. Relevant incorporation of the tracer into normal skin and peritoneal melano­ cytes of Poecilia reticulata (Peters) and Puntius tetrazona tetrazona (Hamilton) could be detected only in rare cases. Melanocytes of malignant melanomas in the hybrids of Xiphophorus helleri (Heckel) and Platypoecilus maculatus (Guenther) exhibited a different degree of radioactivity ac­ cording to their degree of differentiation. The isolation of the melano-protein complex from melanocytes of these melanomas and its subsequent hydrolysis resulted in the following: During melanin synthesis, [35S] sulphate had been incorporated into the chromophore group as an ester-sulphate, whereas no significant incorporation of the tracer was found in the protein matrix. 
  Reference    (Z. Naturforsch. 29c, 42 [1974]; eingegangen am 30. Juli/12. November 1973) 
  Published    1974 
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 TEI-XML for    default:Reihe_C/29/ZNC-1974-29c-0042.pdf 
 Identifier    ZNC-1974-29c-0042 
 Volume    29 
10Author    Hans EcksteinRequires cookie*
 Title    DNA Turnover in Growing Yeast after X-Irradiation  
 Abstract    DNA Turnover, X-Irradiation, Cycloheximide, Yeast Growing yeast cells incorporate radioactivity from labeled nucleotides, deoxynucleotides, deoxy-adenosine, and deoxycytidine into acid-insoluble material. Most of these compounds are in­ corporated predominantly into RNA, only dTMP and dTTP serve as effective and selective DNA precursors. When yeast is prelabeled with dTMP, "specific" radioactivity of D N A decreases in the growing cells due to DNA augmentation. In X-irradiated yeast, "specific" radioactivity of DNA again de­ creases remarkably, though DNA augmentation of the growing giant cells in delayed. Simultaneous­ ly, radioactivity is rendered acid-soluble. Vice versa, X-irradiated unlabeled yeast incorporates radioactivity from labeled precursors into DNA, even if the DNA content of the cells does not increase. When growing yeast cells are treated with cycloheximide, protein synthesis and DNA augmen­ tation are inhibited completely with 5-10~"6 m cycloheximide. The decrease of "specific" radio­ activity of DNA in X-irradiated cells, however, proves independent on an intact protein synthesis, even with 1 • 10—4 M cycloheximide. The data provide clear evidence on DNA turnover in growing yeast after X-irradiation. 
  Reference    (Z. Naturforsch. 29c, 48 [1974]; received October 8 1973) 
  Published    1974 
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 TEI-XML for    default:Reihe_C/29/ZNC-1974-29c-0048.pdf 
 Identifier    ZNC-1974-29c-0048 
 Volume    29 
11Author    Theodor Günther, Wolfgang Pellnitz, Gert MarißRequires cookie*
 Title    Effect of Salts on the Activity and Inhibition of E. coli Membrane ATPase by Ethacrynic Acid and Inhibitors  
 Abstract    Chaotropic Anions, E. coli ATPase, Ethacrynic Acid The [Mg, Ca] -ATPase activity of E. coli depends on the anion present and follows the chao­ tropic sequence: Acetate" > H C 0 3~ > Cl-> I " > N 0 3~ > SCN". There are only small differences between the different alkali chlorides. The [Mg, Ca]-ATPase was inhibited by all these salts when the ratio of Mg or Ca to ATP was 1: 5 at pH 9.1. At pH 9.1 or 7.5 and a Mg to ATP ratio of 1 some salts activate and others inhibit the enzyme according to their position in the chaotropic sequence. W ith Ca the ATPase was activated under these conditions only by acetate. The contradictory activation or inhibition by NaCl or KC1 reported by various authors are due to differences in the Mg: ATP ratios used. The inhibition of ATPase by ethacrynic acid, NEM, pCMB. DCCD and azide is much greater at pH 9.1 than at pH 7.5 and is drastically affected by NaCl or KC1. In E. coli an ATPase which was slightly activated 
  Reference    (Z. Naturforsch. 29c, 54 [1974]; received September 25 1973) 
  Published    1974 
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 TEI-XML for    default:Reihe_C/29/ZNC-1974-29c-0054.pdf 
 Identifier    ZNC-1974-29c-0054 
 Volume    29 
12Author    Theodor Günther, Gert MarißRequires cookie*
 Title    The Membrane ATPase of the (Mg, Ca)-ATPase-Mutant 12, strain AN 120  
 Abstract    (Mg, Ca) -ATPase-Mutant The activity of the membrane ATPase of the (Mg, C a)-ATPase-mutant E. coli K 12 strain AN 120 is reduced to about 5 — 50% of the wildtype ATPase. The reduction is greatest at pH 9.1 and a ratio of Mg, or Ca to ATP of 1 :5 , and smallest at pH 7.5 and 9.1 and a ratio of Mg or Ca to ATP of 1 or more. 
  Reference    (Z. Naturforsch. 29c, 60 [1974]; received September 25 1973) 
  Published    1974 
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 TEI-XML for    default:Reihe_C/29/ZNC-1974-29c-0060.pdf 
 Identifier    ZNC-1974-29c-0060 
 Volume    29 
13Author    Wolfgang KöhnleinRequires cookie*
 Title    Transforming Activity in Both Complementary Strands of Bacillus subtilis DNA  
 Abstract    Transforming Activity, Ultracentrifugation, BrUdR-Sensitization A Bacillus subtilis mutant (strain 168 try" thy-) with a thymine-adenine bias in complementary DNA strands was found. After BrU-incorporation into the DNA it was possible to separate physi cally hybrid DNA-molecules BrU-labeled in different complementary strands using CsCl density gradient centrifugation. These hybrid DNA preparations were used to investigate whether both strands can be informative during transformation. Taking advantage of the selective degradation of BrU-labeled DNA strands by long wavelength UV it was found that the loss of transforming acti­ vity was the same for both hybrid DNA preparations. Thus indicating that both complementary DNA strands can be genetically active during transformation. These results are supported by trans­ formation experiments with denatured and reannealed DNA-molecules and heteroduplex DNA-molecules. 
  Reference    (Z. Naturforsch. 29c, 63 [1974]; received October 29 1973) 
  Published    1974 
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 TEI-XML for    default:Reihe_C/29/ZNC-1974-29c-0063.pdf 
 Identifier    ZNC-1974-29c-0063 
 Volume    29 
14Author    Wolfgang KöhnleinRequires cookie*
 Title    The Influence of Hydrogen Donors on Breakage of Parental DNA Strands and on Biological Activity of Transforming BrU-DNA of B. subtilis after 302/313 nm Radiation  
 Abstract    Long wavelength UV irradiation (302/313 nm) of hybrid and bifilarly labeled BrU-DNA of B. subtilis results in a degradation of the molecular weight of BrU and of parental thymine con­ taining DNA strands. Hydrogen donors present during UV irradiation are able to prevent these "primary" and "secondary" strand breaks. The protection factors are especially large for secondary breaks in parental normal DNA. Through the action of protective agents the biological activity could not be restored. The generation of these primary and secondary strand breaks due to BrU incorporation and the action of hydrogen donors discussed. 
  Reference    (Z. Naturforsch. 29c, 66 [1974]; received October 3/November 23 1973) 
  Published    1974 
  Keywords    BrU-DNA, Hydrogen-Donors, Strand Breaks, Transforming Activity, Radiation 
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 TEI-XML for    default:Reihe_C/29/ZNC-1974-29c-0066.pdf 
 Identifier    ZNC-1974-29c-0066 
 Volume    29 
15Author    B. Dietzschold, 0. R. Kaaden, S. Ueberschaer, F. Weiland, 0. C. StraubRequires cookie*
 Title    Suggestive Evidence for an Oncorna-Virus-Specific DNA Polymerase from C-Type Particles of Bovine Leukosis  
 Abstract    Bovine Leukosis, C-Particles, DNA-Polymerase Typical C-type oncorna virus particles as shown by electron microscopy have been purified from the supernatant of cultured lymphocytes from bovine leukosis. In the purified C-particle fraction a DNA-polymerase activity was detected. Using several synthetic RNA-or DNA-homopolymers and 70S Friend virus R N A the template response of this bovine leukosis cell particle DNA polymerase was compared with those of feline leukaemia virus DNA polymerase and DNA polymerase from normal bovine lymphocytes. The DNA polymerase detected in the viral preparation of bovine leukosis is suggested to be an oncorna-virus-specific enzyme. 
  Reference    (Z. Naturforsch. 29c, 72 [1974]; received September 25/November 16 1973) 
  Published    1974 
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 TEI-XML for    default:Reihe_C/29/ZNC-1974-29c-0072.pdf 
 Identifier    ZNC-1974-29c-0072 
 Volume    29 
16Author    V. Dostal, E. Schauenstein, P. Kulnigg, E. SchmellerRequires cookie*
 Title    Der Einfluß von 4-Hydroxy-2.3-pentenal (HPE) auf die Vermehrung von Vaccinia-Virus in Hühnerfibroblastenkulturen Influence of 4-Hydroxy-2,3-pentenale (HPE) on the Multiplication of Vaccinia-virus in Chick-embryo Fibroblast Cultures  
 Abstract    Vaccinia-virus, Inhibition of DNA Synthesis, 4-Hydroxy-2,3-pentenale The influence of 4-hydroxy-2,3-/rans-pentenale (HPE) on multiplication of Vaccinia virus in chick fibroblast cultures has been investigated. The cytotoxicity of HPE has been estimated by in ­ corporation of [3H] thymidine, [3H] uridine, [3H] leucine and by morphological tests. The greatest influence of H PE was seen after incorporation of [3H] thymidine. Chick-embryo cultures infected with Vaccinia virus showed a marked increase of DNA synthesis in contrast to the controls. This increase averaged 180% after 16 to 14 hours. HPE 0.05-10_ 3 M caused a reduction of 72% in [3H] thymidine incorporation in virus infected cultures. At the same time virus concentration decreased by 76%. HPE 0.1-10~3 M, a concentration which inhibits the normal cell DNA synthesis by 50%, caused about 90% inhibition in infected cultures; virus m ulti­ plication was 99% inhibited. These findings have been attributed to a selective inhibition of virus DNA synthesis by HPE. and possible mechanisms are discussed. 
  Reference    (Z. Naturforsch. 29c, 76 [1974]; eingegangen am 2. Januar/30. Juli 1973) 
  Published    1974 
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 TEI-XML for    default:Reihe_C/29/ZNC-1974-29c-0076.pdf 
 Identifier    ZNC-1974-29c-0076 
 Volume    29 
17Author    J. HölzlRequires cookie*
 Title    Bildung und [14C]-Markierung der  
 Abstract    Flavono­ lignane (Silymarin) bei S ily b u m m a r ia n u m (Haller) Biosynthesis and [14C] Labelling of Flavonolignans (Silymarin) by Silybum marianum (Haller) Flavonolignans, Silymarin Die Früchte von Silybum marianum (Haller) ent­ halten ein Flavonoidgemisch, aus dem bisher neben Taxifolin die drei Flavonolignane Silybin1, Sily-dianin2 und Silychristin3 isoliert und in ihrer Struktur aufgeklärt wurden. Flavonolignane sollen entsprechend ihrer Struktur durch Kondensation aus Taxifolin und Coniferylalkohol entstehen. Pro­ blematisch für Biosyntheseuntersuchungen was bis­ her die Pflanzengröße von 2 bis 3 m. Durch Kulti­ vierung unter besonderen Gewächshausbedingungen erhielt man Pflanzen zwischen 30 und 50 cm Höhe. Obwohl bei den Gewächshauspflanzen die Zeitdauer der Fruchtbildung gegenüber Pflanzen im Freiland verlängert und das Gesamtgewicht der Früchte pro Blütenstand vermindert war, war der Gehalt der Flavonolignane und ihre prozentuale Zusammen­ setzung nicht beeinträchtigt. 
  Reference    (Z. Naturforsch. 29c, 82 [1974]; eingegangen am 28. November 1973) 
  Published    1974 
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 TEI-XML for    default:Reihe_C/29/ZNC-1974-29c-0082_n.pdf 
 Identifier    ZNC-1974-29c-0082_n 
 Volume    29 
18Author    KrishnaC. Joshi, R. K. Bansal, Pahup SinghRequires cookie*
 Title      
 Abstract    We have earlier reported the isolation of lapachol, dehydrotectol, dehydro-a-lapachone, /Mapachone, tectol, /5-sitosterol and paulownin along with d-sesamin from the petroleum ether extract of the heartwood of Phyllarthron comorense DC 1. Paulow­ 
  Reference    (Z. Naturforsch. 29c, 84 [1974]; received July 9 1973) 
  Published    1974 
  Keywords    Paulownin, Phyllarthron Comorense, Mass Spectrum 
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 TEI-XML for    default:Reihe_C/29/ZNC-1974-29c-0084_n.pdf 
 Identifier    ZNC-1974-29c-0084_n 
 Volume    29 
19Author    Heinz Faulstich, Dionysos Georgopoulos, Maria Bloching, Theodor WielandRequires cookie*
 Title    Analysis of the Toxins of Amanitin-Containing Mushrooms  
 Abstract    We have recently developed a method for the analysis of toxins in single specimens of the mush­ room Amanita phalloides (Fr.) Seer.1. Separation of the different toxins was achieved by column chro­ matography, on Sephadex LH20 in water, of a methanolic extract of fresh or dried mushrooms, followed by further purification on thinlayers of silica gel, spectrophotometric evaluation, and, final­ ly, identification of the toxins by amino acid analy­ ses of their y-hydroxylated leucines or isoleucines as lactones. This procedure afforded the quantitative determination of up to 10 toxins in 2 samples of A. phalloides from different sources. 
  Reference    (Z. Naturforsch. 29c, 86 [1974]; received October 25 1973) 
  Published    1974 
  Keywords    Amanitins, Phallotoxins, Toxic Mushrooms, Chromatography 
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 TEI-XML for    default:Reihe_C/29/ZNC-1974-29c-0086_n.pdf 
 Identifier    ZNC-1974-29c-0086_n 
 Volume    29 
20Author    0. Motl, L. DolejšRequires cookie*
 Title    Laserpitium siler L  
 Abstract    Laserpitium siler, 12-Nonacosanone, Mass Spectra During our study on the seed constituents of Laserpitium siler L. we have isolated some time ago a crystalline compound of m.p. 74.5 °C showing the frequency of 1706 cm" 1 in its infrared spec­ trum We have now identified this substance as 12-nonacosanone by mass spectrometry. The high resolution measurement gave for the molecular ion a value of 422.4493 which corresponded to the com­ position C29H580 (calculated 422.4487). The cleav­ age of the ionized molecule in the neighborhood of 
  Reference    (Z. Naturforsch. 29c, 89 [1974]; received September 17 1973) 
  Published    1974 
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 Identifier    ZNC-1974-29c-0089_n 
 Volume    29 
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